Abstract: A liquid discharging device includes a plurality of liquid discharge sections. Each liquid discharge section includes a reservoir, a nozzle that discharges a solution supplied from the reservoir, and discharge energy generating means that generates energy to discharge the solution from the nozzle. The number of the liquid discharge sections corresponds to the number of probe types to be formed. The nozzles are two-dimensionally arranged. Using this liquid discharging device, probe liquids are discharged from the corresponding reservoirs onto a solid-phase substrate to form a predetermined two-dimensional probe array of high-purity probes on the substrate. This process exhibits high reproducibility and processability, and the resulting probe array has high array density.

Abstract: A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of plural base sequences expected to be the unknown base sequence, and fluorescence pattern of a sample on the probe array is compared with template patterns to know the base sequence of the sample.

Abstract: Provided is a method of spotting a probe densely and efficiently on a surface of a solid support. A liquid containing a probe is attached to a solid support as droplets to form spots containing the probe on the solid support by an ink jet method.

Abstract: A reaction site array to be used in conducting a plurality of reactions between two or more kinds of substances in a liquid medium with a trace amount is provided. The reaction site array comprises a plurality of reaction sites separated each other, each of the reaction sites is composed of a first region and a second region, the second region is raised from the first region to separate the first regions each other, and the first region has an affinity to the liquid medium and the second region has an affinity to the liquid medium lower than that of the first region. A preparation process of the reaction site and a reaction process using the reaction site array are also provided.

Abstract: A flat plate-shaped probe carrier for carrying probes such as single stranded DNAs, proteins, etc. which are reactive specifically with target substances comprises a plurality of ring bodies arranged substantially in parallel and the external space among the ring bodies is filled with a filler such that the openings of the ring bodies are oriented to the surface of the probe carrier. Each ring body has a region for fixing a probe adapted to be bonded specifically to a target substance on its inner wall. The probe carrier is produced by bundling a plurality of hollow tubular members in parallel, then filling the external space among the bundled hollow members with a filler, and cutting the bundle along a plane intersecting the axial direction of the tubular members. Probes are fixed to the fixing region before or after filling the external space.

Abstract: A probe carrier of a DNA array or the like is formed by arranging regions fixing different probes in the axial direction of a substrate having an oblong profile which is string-shaped, tape-shaped, string-shaped, tube-shaped or rod-shaped. With this arrangement, the efficiency of manufacturing a probe carrier and handling it in different steps of specimen analysis is improved.

Abstract: Provided is a method of spotting a probe densely and efficiently on a surface of a solid support. A liquid containing a probe is attached to a solid support as droplets to form spots containing the probe on the solid support by an ink jet method.

Abstract: The present invention provides a process for detecting or quantifying a target nucleic acid in a sample, the process comprising the steps of associating a chemiluminescent compound, capable of being associated with a double-stranded nucleic acid, with a double-stranded nucleic acid including the target nucleic acid, and detecting or measuring chemiluminescence derived from the chemiluminescent compound associated with the double-stranded nucleic acid. According to the process, the target nucleic acid in the sample can be highly sensitively detected, or precisely quantified.

Abstract: A probe bound substrate allowing us to quickly detect or quantify a target substance or sequence a target nucleic acid at a lower cost is provided. Specifically, there is provided a probe bound substrate in which a probe capable of specifically attaching to a target substance is bound at the first site on its surface, characterized in that a marker is bound at the second site where the first site may be specified.

Abstract: A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

Abstract: A method for dry detection/quantification of targeted nucleotide chains, comprising the steps of:

Abstract: A novel pyrylium compound is represented by the general formula (I): wherein X is oxygen or sulfur, Y− is a monovalent anion, n is an integer of 2 or 3, and M is hydrogen or an alkali metal. This pyrylium compound functions as a labeling agent by intermolecular bonding to nucleic acids, and as a fluorescence label having a chemical bond with the nucleic acids and an excitation wavelength in a visible light region.

Abstract: DNA extracted from a blood sample by a DNA extractor is provided to a reactor. The reactor makes a hybridization reaction between a DNA microarray on which a plurality of different types of DNA probes are arranged in a predetermined order, and the extracted DNA. A hybridization pattern is formed on the reacted DNA microarray. An authentication certificate is issued by directly attaching the reacted DNA microarray obtained in this way. Alternatively, an authentication certificate is issued by reading the hybridization pattern by a reader, and recording the read pattern as magnetic/digital information.

Abstract: To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light.

Abstract: The present invention provides a process for detecting or quantifying a target nucleic acid in a sample, the process comprising the steps of associating a chemiluminescent compound, capable of being associated with a double-stranded nucleic acid, with a double-stranded nucleic acid including the target nucleic acid, and detecting or measuring chemiluminescence derived from the chemiluminescent compound associated with the double-stranded nucleic acid. According to the process, the target nucleic acid in the sample can be highly sensitively detected, or precisely quantified.

Abstract: The present invention provides pharmaceutical compositions containing a pyrylium compound, a thiopyrylium compound, a selenopyrylium compound or a telluropyrylium compound or a salt of any of the aforesaid compounds as active ingredient. The compounds are selectively absorbed by cancer cells or similar growths in the human or animal body and can be used to bring about distruction of the unwanted growth an irradiation with light of wavelength 600 nm to 1000 nm. The invention also provides a method for the treatment of the human or animal body which comprises administering the compound to a human or animal and irradiating a locus in said animal where the compound is absorbed in order to kill cells at that locus. It further comprises the use of the aforesaid compound for the making of a medicament for use in the treatment of cancer in humans and animals.

Abstract: A method for detecting a target substance in a sample comprises the steps of providing at least two reagents which can form a reaction system for causing changes as the result of an interaction therebetween the interaction being caused only when the target substance is present in the sample, reacting the reagents with the target substance, and measuring the resulting changes based on the interaction, wherein at least one of the reagents forming the reaction system is selected from specific pyrylium compounds.

Abstract: The present invention provide pharmaceutical compositions containing a pyrylium compound, a thiopyrylium compound, a selenopyrylium compound or a telluropyrylium compound or a salt of any of the aforesaid compounds as active ingredient. The compounds are selectively absorbed by cancer cells or similar growths in the human or animal body and can be used to bring about distruction of the unwanted growth on irradiation with light of wavelength 600 nm to 1000 nm. The invention also provides a method for the treatment of the human or animal body which comprises administering the compound to a human or animal and irradiating a locus in said animal where the compound is absorbed in order to kill cells at that locus. It further comprises the use of the aforesaid compound for the making of a medicament for use in the treatment of cancer in humans and animals.

Abstract: A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R.sup.1, R.sup.2 and R.sup.3, wherein each of R.sup.1 and R.sup.2 is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R.sup.3 is -A or -L-A, L is -L.sup.1 -, -L.sup.2 -L.sup.3 - or -L.sup.4 -L.sup.5 -L.sup.6 -, and each of L.sup.1 to L.sup.6 is independently --(CH.dbd.CH)--, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y.sup.- is an anion.

Abstract: A method for detecting a nucleic acid hybrid comprises steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid, wherein the step for detecting the double helical structure comprises incorporating, into the sample solution, two or more kinds of reagents which are capable of causing a detectable change by interaction therebetween through the double helical structure and measuring the change caused by the interaction of the reagents; and at least one of the two or more kinds of reagents is joined to the probe.