Abstract: The present invention relates to stem cell-derived microvesicles with enhanced efficacy, a use thereof, and a method for enhancing efficacy, and more particularly, to a use of stem cell-derived microvesicles with an enhanced expression level of microRNAs for the prevention or treatment of stroke, and a method for promoting the production of microRNAs of stem cell-derived microvesicles and enhancing efficacy, and a method for promoting the production of stem cell-derived microvesicles and microRNAs within the microvesicles and enhancing the efficacy of stem cells and microvesicles thereof by 3-dimensionally culturing or ischemically stimulating stem cells.

Abstract: A method for manufacturing a cylinder head includes: preparing a cylinder head casting having an intake passage, an exhaust passage, and a combustion chamber by casting using a mold and a plurality of cores; machining an intake port, an intake valve seat, an intake valve guide bore, an exhaust port, an exhaust valve seat, and an exhaust valve guide bore in the cylinder head casting by a first cylindrical tool; and forming a tapered surface on a portion of edge of the intake port by a second cylindrical tool. In particular, the second cylindrical tool moves along a predetermined trajectory at the edge portion of the intake port and rotates around an axis simultaneously to machine the tapered surface.

Abstract: Disclosed herein is an injectable anticancer composition for local administration, which contains a suspension of quinine hydrochloride. The anticancer composition for local administration according to the present invention shows an IC50 value against MKN-45 cells, which is about 10 times lower than Paclitaxel, as determined by an MTT assay in vitro, suggesting that the anticancer composition has an excellent cytotoxic effect. The anticancer composition can be administered as a safe anticancer agent in clinical applications, and also shows an anticancer effect by inducing the necrosis and detachment of solid cancer cells. Particularly, the anticancer composition has an anticancer mechanism by which the composition acts locally in a tumor tissue administered with the composition and shows a rapid antitumor effect (1-2 weeks after administration).

Abstract: The present invention relates to a composition including stem cell-derived microvesicles as an active ingredient for promoting neurogenesis. The stem cell-derived microvesicles according to the present invention can promote neurogenesis and migration of nerves and also promote angiogenesis in vascular endothelial cells, and thus can be usefully used in treatment of neurological damage.

Abstract: Provided are a method for producing stem cell-derived extracellular vesicles by using a three-dimensional cell culture process, use of three-dimensional cell aggregates of stem cells in producing extracellular vesicles, a culture of three-dimensional cell aggregates of stem cells comprising a high concentration of extracellular vesicles, and a pharmaceutical composition comprising the culture.

Abstract: The present invention relates to a composition for topically delivering SDF-1 into the brain, in which the composition comprises a dual ionic pH-sensitive copolymer and a nerve regeneration and protective factor. In the present invention, when a dual ionic pH-sensitive copolymer containing SDF-1 as a nerve regeneration and protective factor is applied to a patient suffering from ischemic stroke as a drug carrier, it induces the effective delivery of the treatment factor to a topical lesion site, and moreover, the risk factors for adverse effects may be cancelled out, so that it can be effectively used as a novel therapeutic agent for ischemic brain diseases.

Abstract: The present invention relates to a composition comprising an ischemic serum for promoting the activation of a stem cell, to a method for promoting the activation of a stem cell and to a stem cell, the activation of which is promoted by the composition or the method. The ischemic serum according to the present invention may stimulate the stem cell to facilitate the secretion of a growth factor, improve survival rate, improve mobility to an injured part, improve proliferation rate and maintain characteristics of the stem cell, thus promoting an activation of the stem cell such that the stem cell becomes suitable for transplantation of the stem cell to a patient having a brain injury.