Abstract: Techniques are described to characterize motion patterns of a group of agents engaging in an activity. An analysis system receives input data associated with spatial and temporal information of at least one element of interest associated with the activity, where the object of interest may be a ball, person, animal or any other object in motion. The analysis system partitions the input data into a plurality of spatiotemporal segments and generates one or more representations of one or more sets of segments of the plurality of spatiotemporal segments based on one or more criteria. The analysis system computes a metric, such as an entropy value, for each of the one or more representations. Partial tracing data, such as ball movements in a sporting event, may be created using an inexpensive input device, such as a tablet computer, making the disclosed techniques available for a wide range of events and activities.

Abstract: In a method for generating a long nucleic acid molecule, nucleic acids immobilized on a surface and having overlapping complementary sequences is released into solution. The overlapping complementary sequences are hybridized to form hybridized nucleic acids, followed by extension or ligation of the hybridized nucleic acids to synthesize the long nucleic acid molecule. The nucleic acids may comprise first and second series of nucleic acids having redundant overlapping sequences, wherein nucleic acids from the first and second series are complementary to each other. The complementary nucleic acids are hybridized to form the hybridized nucleic acids. The generated long nucleic acid molecule may have a predetermined sequence element, and it may be introduced into a system wherein the predetermined sequence element is required for replication, such that replication of the synthesized long nucleic acid molecule is indicative of the presence of the predetermined sequence element in the long nucleic acid molecule.

Abstract: In a method for synthesizing a pool of nucleic acid molecules, a first nucleic acid has a first 5? region and a first 3? region and a second nucleic acid has a second 5? region and a second 3? region. The second 3? region and the first 5? region have identical nucleic acid sequences. The first 3? region is hybridized with an oligonucleotide, extending the hybridized oligonucleotide and producing a first extension product having a 3? region complementary to the first 5? region. The second nucleic acid is hybridized with the first extension product to hybridize the 3? region of the first extension product to the second 3? region, extending the 3? region of the first extension product and producing a second extension product having a 3? region complementary to the second 5? region. Error-containing molecules are separated from error-free molecules by a component that selects for a sequence error.

Abstract: The invention concerns image processing that includes the estimation of the optical depths of points in image data in order to enhance the image data. In particular but not limited to, from the optical depth noise present in the image due to aerosol in the scene, such as fog or haze, can be reduced. The invention concerns determining.a first cost for each point independently based on a determined metric (60) and then a second cost based on an expected correlation in neighbouring points (70). The two costs are then optimized (80) to determine an estimated optical depth for each point (80) that is then used to reduce the effect of aerosol in the image (84). Applications of the invention include, but is not limited to, surveillance systems so that objects in the scene of the image can be better identified once the image data has been enhanced to reduce the estimated noise. Aspects of the invention include a method, software, computer apparatus and a field programmable gate array.

Abstract: A method for synthesizing a nucleic acid having a desired sequence and length comprises providing a solid support having an immobilized nucleic acid, performing a nucleic acid addition reaction to elongate the immobilized nucleic acid by adding a nucleotide or an oligonucleotide attached to a protecting group to the nucleic acid, determining whether the nucleotide or the oligonucleotide is added to the nucleic acid, removing the protecting group, and continuing until the immobilized nucleic acid has a desired sequence and length.

Abstract: In a method for synthesizing a pool of nucleic acid molecules, a first nucleic acid has a first 5? region and a first 3? region and a second nucleic acid has a second 5? region and a second 3? region. The second 3? region and the first 5? region have identical nucleic acid sequences. The first 3? region is hybridized with an oligonucleotide, extending the hybridized oligonucleotide and producing a first extension product having a 3? region complementary to the first 5? region. The second nucleic acid is hybridized with the first extension product to hybridize the 3? region of the first extension product to the second 3? region, extending the 3? region of the first extension product and producing a second extension product having a 3? region complementary to the second 5? region. Error-containing molecules are separated from error-free molecules by a component that selects for a sequence error.

Abstract: A method for synthesizing a nucleic acid having a desired sequence and length comprises providing a solid support having an immobilized nucleic acid, performing a nucleic acid addition reaction to elongate the immobilized nucleic acid by adding a nucleotide or an oligonucleotide to the nucleic acid, determining whether the nucleotide or the oligonucleotide is added to the nucleic acid by detecting whether there is an increase in electrophoretic force applied to the solid support when an electric field and a magnetic field gradient are applied to the support, wherein the increase in electrophoretic force applied to the support is caused by adding the nucleotide or the oligonucleotide to the nucleic acid, repeating the addition reaction and determination steps if the nucleotide or the oligonucleotide is not added to the nucleic acid, and continuing until the immobilized nucleic acid has a desired sequence and length.

Abstract: In a method for synthesizing a long nucleic acid molecule, a first immobilized nucleic acid has a first 5? region and a first 3? region and a second immobilized nucleic acid has a second 5? region and a second 3? region. The second 3? region and the first 5? region have identical nucleic acid sequences. An oligonucleotide is hybridized to the first 3? region, extending the hybridized oligonucleotide and producing a first extension product having a 3? region that is complementary to the first 5? region. The 3? region of the first extension product is hybridized to the second 3? region, extending the 3? region of the first extension product and producing a synthesized nucleic acid molecule having a 3? region that is complementary to the second 5? region, wherein the synthesized nucleic acid molecule has a sequence complementary to the first and second 3? and 5? regions.

Abstract: A method for synthesizing a nucleic acid having a desired sequence and length comprises providing a solid support having an immobilized nucleic acid, performing a nucleic acid addition reaction to elongate the immobilized nucleic acid by adding a nucleotide or an oligonucleotide to the nucleic acid, determining whether the nucleotide or the oligonucleotide is added to the nucleic acid by detecting whether there is an increase in electrophoretic force applied to the solid support when an electric field and a magnetic field gradient are applied to the support, wherein the increase in electrophoretic force applied to the support is caused by adding the nucleotide or the oligonucleotide to the nucleic acid, repeating the addition reaction and determination steps if the nucleotide or the oligonucleotide is not added to the nucleic acid, and continuing until the immobilized nucleic acid has a desired sequence and length.

Abstract: Inhibitors of HIV membrane fusion and a method of identifying drugs or agents which inhibit binding of the N-helix coiled-coil and the C helix of HIV gp41 envelope protein.

Abstract: In a method for synthesizing a long nucleic acid molecule, a first immobilized nucleic acid has a first 5? region and a first 3? region and a second immobilized nucleic acid has a second 5? region and a second 3? region, wherein the second 3? region and the first 5? region have identical nucleic acid sequences. The first immobilized nucleic acid is hybridized with an oligonucleotide under conditions promoting hybridization of the oligonucleotide to the first 3? region, extending the hybridized oligonucleotide and producing a first extension product having a 3? region that is complementary to the first 5? region.

Abstract: This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.

Abstract: This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.

Abstract: A system and method of managing numerous facets of work relationships. In a preferred form, the system provides end-to-end integration of the contract resourcing process (“requisition to check”) for a multitude of clients and a multitude of service providers.

Abstract: Inventive aspects of the present disclosure generally relates to fuel cells and, in particular, to fuel cells that can use microorganisms (microbes) to oxidize fuel. Certain aspects are directed to fuel cells that operate at relatively elevated temperatures. Such temperatures, for example, can increase the metabolisms of the microorganisms within the fuel cell. The elevated temperatures may be achieved, for instance, by using a thermal insulator, such as a vacuum jacket. Microorganism metabolism may also be improved, in some aspects of the invention, by exposing the microorganisms to growth promoters such as fertilizer, nitrogen sources, biomass, etc. The microorganisms, in some embodiments of the invention, may be anaerobic or microaerophilic and can be obtained, for example, from the soil, compost, peat, sewage, bogs, wastewater, or other organic-rich matrices. Another inventive aspect relates to novel electrodes for use in fuel cells, such as microbial fuel cells.

Abstract: A read head for a scale reading apparatus, the head including a light source and an array of photodetector elements, wherein said light source and array of photodetector elements are fabricated in a lattice matched semiconductor compound.

Abstract: A method and instrument for raising finance from generating and trading identifiable plural ownership rights in assets, such as intellectual property, or other properties and any associated rights or benefits, such as profit streams. An exemplary method comprising generating a plurality of identifiable ownership rights and facilitating the buying and selling of said identifiable rights to members of the public and others. An exemplary instrument comprises a plurality of identifiable ownership rights enabling plural ownership of an asset or property and any associated rights or benefits by members of the public and other entities. The asset or property may be defined to be intellectual property or some other property, benefit or other associated right owned by a seller, member of the public or other entity and capable of generating a profit stream or some other benefit. This methods advantages include raising finance for sellers, and enabling the public to participate.

Abstract: A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol are applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

Abstract: This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.

Abstract: This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.