Abstract: A feed control system and related methods for a heterologous product, such as a human milk oligosaccharide, fermentation system are disclosed. The feed control system comprises a feed controller. The feed controller comprises a processor and an interface. The processor is configured to obtain a biomass content in a fermentation broth. The processor is configured to determine a carbon input parameter based on the biomass content. The processor is configured to control a carbon input to the fermentation broth according to the carbon input parameter.

Abstract: The present disclosure relates to the production of oligosaccharides, especially Human milk Oligosaccharides (HMOs) using a genetically engineered cell which has decreased or total loss of function of phosphoglycerol transferase I and II and/or phosphoethanolamine transferase and/or glucans biosynthesis protein C to reduce oligosaccharide by-products and/or increase oligosaccharide production.

Abstract: The present disclosure relates to a method for the production of a human milk oligosaccharide (HMO) blend with LNFP-I and 2?-FL as the predominant HMO's, the method comprising the steps of providing a genetically engineered cell, which comprises a heterologous ?-1,3-N-acetyl-glucosaminyltransferase protein, a heterologous ?-1,3-galactosyltransferase protein, a heterologous ?-1,2-fucosyltransferase protein, and expresses functionally the colanic acid gene cluster, comprises a native or heterologous regulatory or episomal element for controlling the expression of the proteins and optionally express a heterologous sugar transporter, and culturing the cell in a suitable cell culture medium to express said proteins and to produce an HMO blend.

Abstract: This invention relates to a method of producing mixtures of various human milk oligosaccharides (HMOs) with unique HMO blend profiles, consisting predominantly of LNFP-I and LNT and of other HMOs in less significant amounts. The less abundant HMOs might be 2?-FL, LNT-II or DFL. The strategies for achieving specific HMO blends include strain engineering and fermentation methods.

Abstract: A feed control system and related methods for a heterologous product, such as a human milk oligosaccharide, fermentation system are disclosed. The feed control system comprises a feed controller. The feed controller comprises a processor and an interface. The processor is configured to obtain a biomass content in a fermentation broth. The processor is configured to determine a carbon input parameter based on the biomass content. The processor is configured to control a carbon input to the fermentation broth according to the carbon input parameter.

Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Abstract: An odor eliminating pet bed assembly for absorbing odors associated with pet beds includes a pet bed that is positionable on a support surface for to have a pet lie thereon. The pet bed has a chamber is integrated therein for receiving an odor absorbing material. A permeable cushion is positioned within the pet bed to have the pet lie thereon when the pet is in the pet bed. The permeable cushion passes the odor absorbing material therethrough. An impermeable cushion is positioned within the pet bed to have the pet lie thereon. The impermeable cushion is comprised of a fluid impermeable material to inhibit the odor absorbing material from passing therethrough.

Abstract: An odor eliminating pet bed assembly for absorbing odors associated with pet beds includes a pet bed that is positionable on a support surface for to have a pet lie thereon. The pet bed has a chamber is integrated therein for receiving an odor absorbing material. A permeable cushion is positioned within the pet bed to have the pet lie thereon when the pet is in the pet bed. The permeable cushion passes the odor absorbing material therethrough. An impermeable cushion is positioned within the pet bed to have the pet lie thereon. The impermeable cushion is comprised of a fluid impermeable material to inhibit the odor absorbing material from passing therethrough.

Abstract: An assistance method for a light control system in a motor vehicle, having a light controller for controlling a lighting unit in the motor vehicle for lighting a motor vehicle environment, a portable unit, at least one communication interface, and an evaluation unit for evaluating signals from the portable unit received via the communication interface. The lighting unit being controllable by the light controller and in accordance with the evaluation of the signals received from the portable unit in a first assistance function, such that at least one light beam emitted by the lighting unit can be positioned as a function of the position of the portable unit. The lighting unit is controlled via the light controller, such that when the first assistance function is switched on, the light beam emitted by the lighting unit automatically tracks the portable unit in accordance with the motion trajectory of the portable unit.

Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Abstract: The present invention is directed to methods for the separation of a molecule of interest from a solution containing the molecule using dual stage tangential-flow ultrafiltration (“TFF”). In particular, the methods of the invention are directed to the processing of crude feed streams such as conditioned cell culture supernatant to dramatically reduce contaminant and/or impurity levels prior to subsequent, i.e., downstream, refining unit operations. The methods of the invention may be used in the processing of a crude feed stream from biological production systems such as fermentation or other cell culture process, and may further eliminate the need for time consuming impurity precipitation (e.g., pH driven) and/or precipitate filtration processes prior to downstream processes that are sensitive to high impurity loads such as chromatographic unit operations.

Abstract: An assistance method for a light control system in a motor vehicle, having a light controller for controlling a lighting unit in the motor vehicle for lighting a motor vehicle environment, a portable unit, at least one communication interface, and an evaluation unit for evaluating signals from the portable unit received via the communication interface. The lighting unit being controllable by the light controller and in accordance with the evaluation of the signals received from the portable unit in a first assistance function, such that at least one light beam emitted by the lighting unit can be positioned as a function of the position of the portable unit. The lighting unit is controlled via the light controller, such that when the first assistance function is switched on, the light beam emitted by the lighting unit automatically tracks the portable unit in accordance with the motion trajectory of the portable unit.

Abstract: An assistance method for a light control system in a motor vehicle, having a light controller for controlling a lighting unit in the motor vehicle for lighting a motor vehicle environment, a portable unit, at least one communication interface, and an evaluation unit for evaluating signals from the portable unit received via the communication interface. The lighting unit being controllable by the light controller and based on evaluation of the signals received from the portable unit, such that at least one light beam emitted by the lighting unit can be positioned in accordance with signals from the portable unit. A first assistance function manually or automatically switches on, the lighting unit is controlled such that when the first assistance function is switched on, the light beam moves automatically as a function of the portable unit being actuated at a time that follows the switching on of the first assistance function.

Abstract: Systems, methods, and computing devices for building, operating and using map systems with signature-based map caches are disclosed. Based on the desired map properties included in a formatted map request, a map signature can be generated. The map signature can be used to access a signature based map cache to determine whether a map with identical map properties has been previously generated and stored in cache or otherwise available for retrieval. If the map signature exists in the map cache, the corresponding map can be delivered to a map client. If the map signature does not exist in the map cache, then a map can be generated or rendered based on the map properties in the map request. Once the map is generated, it can be saved to the map cache along with associated map signature for future retrieval.

Abstract: Embodiments of the invention relate to an image or raster compression method for a multi-dimensional array of pixels. A user specifies a maximum error allowable per pixel for the compression algorithm. The raster is divided into a number of pixel blocks where each pixel is quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The size of the pixel blocks is limited to two (e.g., 8×8 and 16×16) to save processing time with little difference in compression. A Look-Up Table (LUT) is used instead for certain types of data where it is more efficient.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.