Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: Embodiments of the invention relate to an image or raster compression method that includes receiving pixel data for a raster comprising a two dimensional (2D) array of pixels where each pixel is associated with a data value. The method further includes receiving a user defined parameter defining a maximum error allowable per pixel for a compression algorithm. The raster can be divided into a number of pixel blocks where each pixel can be quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The method further includes executing the compression algorithm wherein for each pixel, where an error caused by the compression algorithm is equal to or less than the maximum error allowable, and encoding the pixel data based on the compression algorithm. In certain embodiments, the compression algorithm is a non-transform compression algorithm.

Abstract: Embodiments of the invention relate to an image or raster compression method for a multi-dimensional array of pixels. A user specifies a maximum error allowable per pixel for the compression algorithm. The raster is divided into a number of pixel blocks where each pixel is quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The size of the pixel blocks is limited to two (e.g., 8×8 and 16×16) to save processing time with little difference in compression. A Look-Up Table (LUT) is used instead for certain types of data where it is more efficient.

Abstract: Embodiments of the invention relate to an image or raster compression method that includes receiving pixel data for a raster comprising a two dimensional (2D) array of pixels where each pixel is associated with a data value. The method further includes receiving a user defined parameter defining a maximum error allowable per pixel for a compression algorithm. The raster can be divided into a number of pixel blocks where each pixel can be quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The method further includes executing the compression algorithm wherein for each pixel, where an error caused by the compression algorithm is equal to or less than the maximum error allowable, and encoding the pixel data based on the compression algorithm. In certain embodiments, the compression algorithm is a non-transform compression algorithm.

Abstract: The present invention is directed to methods for the separation of a molecule of interest from a solution containing the molecule using dual stage tangential-flow ultrafiltration (“TFF”). In particular, the methods of the invention are directed to the processing of crude feed streams such as conditioned cell culture supernatant to dramatically reduce contaminant and/or impurity levels prior to subsequent, i.e., downstream, refining unit operations. The methods of the invention may be used in the processing of a crude feed stream from biological production systems such as fermentation or other cell culture process, and may further eliminate the need for time consuming impurity precipitation (e.g., pH driven) and/or precipitate filtration processes prior to downstream processes that are sensitive to high impurity loads such as chromatographic unit operations.

Abstract: Embodiments of the invention relate to an image or raster compression method that includes receiving pixel data for a raster comprising a two dimensional (2D) array of pixels where each pixel is associated with a data value. The method further includes receiving a user defined parameter defining a maximum error allowable per pixel for a compression algorithm. The raster can be divided into a number of pixel blocks where each pixel can be quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The method further includes executing the compression algorithm wherein for each pixel, where an error caused by the compression algorithm is equal to or less than the maximum error allowable, and encoding the pixel data based on the compression algorithm. In certain embodiments, the compression algorithm is a non-transform compression algorithm.

Abstract: Systems, methods, and computing devices for building, operating and using map systems with signature-based map caches are disclosed. Based on the desired map properties included in a formatted map request, a map signature can be generated. The map signature can be used to access a signature based map cache to determine whether a map with identical map properties has been previously generated and stored in cache or otherwise available for retrieval. If the map signature exists in the map cache, the corresponding map can be delivered to a map client. If the map signature does not exist in the map cache, then a map can be generated or rendered based on the map properties in the map request. Once the map is generated, it can be saved to the map cache along with associated map signature for future retrieval.

Abstract: Systems, methods, and computing devices for building, operating and using map systems with signature-based map caches are disclosed. Based on the desired map properties included in a formatted map request, a map signature can be generated. The map signature can be used to access a signature based map cache to determine whether a map with identical map properties has been previously generated and stored in cache or otherwise available for retrieval. If the map signature exists in the map cache, the corresponding map can be delivered to a map client. If the map signature does not exist in the map cache, then a map can be generated or rendered based on the map properties in the map request. Once the map is generated, it can be saved to the map cache along with associated map signature for future retrieval.

Abstract: In a method and a device for traffic sign recognition, at least one significant feature for a traffic sign is determined which is standardized for a region. The region that corresponds to the determined feature is determined. At least one classification feature and/or at least one classification method is defined depending on the determined region for the recognition of the traffic sign and/or at least one further traffic sign. The recognition of the traffic sign and/or the at least one further traffic sign is performed by using the defined classification feature and/or the defined classification method.

Abstract: Embodiments of the invention relate to an image or raster compression method that includes receiving pixel data for a raster comprising a two dimensional (2D) array of pixels where each pixel is associated with a data value. The method further includes receiving a user defined parameter defining a maximum error allowable per pixel for a compression algorithm. The raster can be divided into a number of pixel blocks where each pixel can be quantized and bit stuffed based on a number of block statistics including the maximum error allowable. The method further includes executing the compression algorithm wherein for each pixel, where an error caused by the compression algorithm is equal to or less than the maximum error allowable, and encoding the pixel data based on the compression algorithm. In certain embodiments, the compression algorithm is a non-transform compression algorithm.

Abstract: A method for reducing or substantially preventing formation of a trisulfide derivative of a polypeptide in a liquid medium containing the polypeptide ijn question comprises stripping the liquid medium with a gas, suitably a chemically unreactive gas such as nitrogen or argon.

Abstract: The invention relates to a magnetic levitation vehicle (11) and to a method for lifting said magnetic levitation vehicle (11) and to lowering said magnetic levitation vehicle onto a roadway. According to the invention, the carrier magnetic units (38) of the magnetic levitation vehicle (11) are not all activated or deactivated at the same time but at different times in order to keep the current energy requirement from an external energy supply or main power supply to a minimum.

Abstract: Systems, methods, and computing devices for building, operating and using map systems with signature-based map caches are disclosed. Based on the desired map properties included in a formatted map request, a map signature can be generated. The map signature can be used to access a signature based map cache to determine whether a map with identical map properties has been previously generated and stored in cache or otherwise available for retrieval. If the map signature exists in the map cache, the corresponding map can be delivered to a map client. If the map signature does not exist in the map cache, then a map can be generated or rendered based on the map properties in the map request. Once the map is generated, it can be saved to the map cache along with associated map signature for future retrieval.

Abstract: The invention relates to a magnetic levitation vehicle (11) and to a method for lifting said magnetic levitation vehicle (11) and to lowering said magnetic levitation vehicle onto a roadway. According to the invention, the carrier magnetic units (38) of the magnetic levitation vehicle (11) are not all activated or deactivated at the same time but at different times in order to keep the current energy requirement from an external energy supply or main power supply to a minimum.

Abstract: A device for transmitting electrical energy from the track (2, 3) to the vehicle (1) of a magnetic leviation railway is described. According to the invention a contactless operating device is provided in that the track (2, 3) has mounted thereon at least one primary line (44) connected to a voltage source (46) and being designed as an emitting coil and in that the vehicle (1) has mounted thereon at least one pick-up coil (47) applied to its magnet back box (15).

Abstract: In a method and a device for traffic sign recognition, at least one significant feature for a traffic sign is determined which is standardized for a region. The region that corresponds to the determined feature is determined. At least one classification feature and/or at least one classification method is defined depending on the determined region for the recognition of the traffic sign and/or at least one further traffic sign. The recognition of the traffic sign and/or the at least one further traffic sign is performed by using the defined classification feature and/or the defined classification method.

Abstract: A magnet arrangement for a magnetic levitation vehicle is described. The magnet arrangement comprises a plurality of magnet poles (11) being combined to form groups (11a to 11f), wherein according to the invention each group contains one magnet pole (11a to 11f) or two magnet poles (11a to 11f). The windings (12a to 12f) of each group are controlled by a control circuit (18) individually assigned to it (FIG. 8).

Abstract: A method for reducing or substantially preventing formation of a trisulfide derivative of a polypeptide in a liquid medium containing the polypeptide ijn question comprises stripping the liquid medium with a gas, suitably a chemically unreactive gas such as nitrogen or argon.

Abstract: A digital input/output unit has a series of terminal points and visual display devices assigned to the terminal points for the display of the respective switching state. It also has an interface for the exchange of process data with a controller and an electronic circuit connected between the interface and the respective terminal points for converting the process data into corresponding switching signals and vice versa. The electronic circuit has an evaluation device for terminal point related signaling data received from the interface in order to activate a respective selected visual display device regardless of the current switching state. Alternatively, or in addition, the electronic circuit can have an evaluation device for a common signaling datum, which can be received from the interface, relative to the complete input/output unit in order to be able to jointly activate all visual display devices regardless of the particular current switching state.

Abstract: A method for configuring or reconfiguring an automation device and an automation device suitable for a configuration or a reconfiguration are provided. The automation device has a programmable controller (14) with a CPU unit (14a) and decentralized peripheral devices (16, 17, 18). In this method and automation device, newly supplied configuration data can be evaluated for changes in the hardware configuration and can be integrated during a configuration or a reconfiguration interval (21) without the automation device having to switch to a STOP mode. As a result, it is not necessary for centralized and/or decentralized peripheral modules to adopt a controlled (safe) state, which means that control can be resumed immediately after the reconfiguration.