Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Abstract: A device for coupling to a fluid connector of an IV administration set to resist contamination of the fluid connector and IV administration set and permit priming of the IV administration set. The device including a cover body having a cavity for receiving the fluid connector, a priming passage for priming fluid from the IV administration set through the fluid connector, and a coupling tab extending from the cover body, the coupling tab permitting the cover body to be coupled to a length of an IV line or other portion of the IV administration set to resist contamination and permit priming of the IV administration set.

Abstract: The present invention relates to cytolytic bi-component protein complexes consisting of a plurality of molecules of a member of the aegerolysin family and a plurality of molecules of a member of the MACPF superfamily, and particularly to their use for controlling a plant pest, such as for controlling Colorado potato beetle (Leptinotarsa decemlineata) or Western corn rootworm (Diabrotica virgifera virgifera). More specifically, the invention relates to cytolytic bi-component protein complexes formed by a plurality of molecules of one of the aegerolysins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) with a plurality of molecules of pleurotolysin B (PlyB) or similar proteins, which have been shown to be toxic for the aforementioned agricultural pest insects.

Abstract: A device for coupling to a fluid connector of an IV administration set to resist contamination of the fluid connector and IV administration set and permit priming of the IV administration set. The device including a cover body having a cavity for receiving the fluid connector, a priming passage for priming fluid from the IV administration set through the fluid connector, and a coupling tab extending from the cover body, the coupling tab permitting the cover body to be coupled to a length of an IV line or other portion of the IV administration set to resist contamination and permit priming of the IV administration set.

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Abstract: A device for coupling to a fluid connector of an IV administration set to resist contamination of the fluid connector and IV administration set and permit priming of the IV administration set. The device including a cover body having a cavity for receiving the fluid connector, a priming passage for priming fluid from the IV administration set through the fluid connector, and a coupling tab extending from the cover body, the coupling tab permitting the cover body to be coupled to a length of an IV line or other portion of the IV administration set to resist contamination and permit priming of the IV administration set.

Abstract: A device for coupling to a fluid connector of an IV administration set to resist contamination of the fluid connector and IV administration set and permit priming of the IV administration set. The device including a cover body having a cavity for receiving the fluid connector, a priming passage for priming fluid from the IV administration set through the fluid connector, and a coupling tab extending from the cover body, the coupling tab permitting the cover body to be coupled to a length of an IV line or other portion of the IV administration set to resist contamination and permit priming of the IV administration set.

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract: The technology relates to a neural network dual in-line memory module (NN-DIMM), a microelectronic system comprising a CPU and a plurality of the NN-DIMMs, and a method of transferring information between the CPU and the plurality of the NN-DIMMS. The NN-DIMM may include a module card having a plurality of parallel edge contacts adjacent to an edge of a slot connector thereof and configured to have the same command and signal interface as a standard dual in-line memory module (DIMM). The NN-DIMM may also include a deep neural network (DNN) accelerator affixed to the module card, and a bridge configured to transfer information between the DNN accelerator and the plurality of parallel edge contacts via a DIMM external interface.

Abstract: Method and apparatus for detecting vibrational and/or acoustic transfers in a mechanical system A method and apparatus for detecting vibro-acoustic transfers in a mechanical system are provided. The method comprises: while operating the mechanical system, acquiring, at each of multiple input points, an input signal indicative of a mechanical load acting on the input point, and acquiring, at a response point, a response signal indicative of a mechanical response; training a neural network device using the input signals acquired at the input points and using the response signal acquired at the response point; and, for each of the input points: providing only the input signal acquired at the respective input point to the trained neural network device and obtaining, from the neural network device, a contribution signal indicative of a predicted contribution of the respective input signal to the response signal.

Abstract: A non-spray-dried, dry-granulated ceramic particulate mixture including at least 40 wt % coal combustion fly ash and from 4 wt % to 9 wt % water. At least 90 wt % of the particles have a particle size of from 80 ?m to 600 ?m.

Abstract: A non-spray-drying, dry-granulation process for making a ceramic particulate mixture including from 4 wt % to 9 wt % water. At least 90 wt % of the particles have a particle size of from 80 ?m to 600 ?m. The process includes the steps of: (a) forming a precursor material; (b) subjecting the precursor material to a compaction step to form a compacted precursor material; (c) subjecting the compacted precursor material to a crushing step to form a crushed precursor material; and (d) subjecting the crushed precursor material to at least two air classification steps. One air classification step removes at least a portion of the particles having a particle size of greater than 600 ?m from the crushed precursor material, and the other air classification step removes at least a portion of the particles having a particle size of less than 80 ?m from the crushed precursor material.

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract: Method and apparatus for detecting vibrational and/or acoustic transfers in a mechanical system A method and apparatus for detecting vibro-acoustic transfers in a mechanical system are provided. The method comprises: while operating the mechanical system, acquiring, at each of multiple input points, an input signal indicative of a mechanical load acting on the input point, and acquiring, at a response point, a response signal indicative of a mechanical response; training a neural network device using the input signals acquired at the input points and using the response signal acquired at the response point; and, for each of the input points: providing only the input signal acquired at the respective input point to the trained neural network device and obtaining, from the neural network device, a contribution signal indicative of a predicted contribution of the respective input signal to the response signal.