Abstract: Methods for performing a dynamic analysis of complex systems are described. The dynamic analysis of complex systems is performed by sub-structuring of the system into a plurality of sub-structures. The sub-structures and the interfaces between the sub-structures are modelled using a generalized eigenvector analysis of a discretized model of a full system or a subassembly thereof comprising at least two sub-structures. The sub-structures then are combined at the interfaces into a combined system. The combined system then is solved. Optionally, selected sub-structures may be reduced. Performing such a dynamic analysis allows studying characteristics such as e.g. vibration and/or acoustical effects in a computational efficient way. Such methods can be applied for optimizing designs of such complex systems.

Abstract: A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.

Abstract: A matchline sense circuit for detecting a rising voltage on a matchline of a CAM array is disclosed. The circuit initially precharges a matchline to ground before turning on a current source to supply current to the matchline and raise the voltage of the matchline. A reference matchline sense circuit generates a self-timed control signal to keep the current supply turned on for a predetermined duration of time. Sensed data on the matchlines are latched after the current source is turned off and the matchlines are precharged to ground. Because the matchline sense circuit of the present invention precharges the matchlines to ground instead of the supply voltage, VDD, less power is consumed. By sensing the rise of the matchline voltage to an n-channel transistor threshold potential, the matchline sensing operation speed is increased.

Abstract: A method for forming a porous three-dimensional (3-D) object includes creating a mold from a negative replica of the 3-D object, the 3-D object having a first size and at least one predetermined feature, and then casting a flowable material into and/or onto the mold. The method further includes forming pores of a second size and/or a third size in the flowable material, thereby forming the porous 3-D object.

Abstract: A multiple matchline sense circuit for detecting a single, more than one, or no match conditions during a search-and-compare operation of a content addressable memory is disclosed. The circuit compares the rising voltage rate of a multiple matchline to the rising voltage rate of a reference multiple matchline in order to generate a multibit result representing one of the three conditions. The circuit generates a self-timed control signal to end the search-and-compare operation, and to set the circuit to a precharge state.

Abstract: Methods for performing a dynamic analysis of complex systems are described. The dynamic analysis of complex systems is performed by sub-structuring of the system into a plurality of sub-structures. The sub-structures and the interfaces between the sub-structures are modelled using a generalised eigenvector analysis of a discretised model of a full system or a subassembly thereof comprising at least two sub-structures. The sub-structures then are combined at the interfaces into a combined system. The combined system then is solved. Optionally, selected sub-structures may be reduced. Performing such a dynamic analysis allows studying characteristics such as e.g. vibration and/or acoustical effects in a computational efficient way. Such methods can be applied for optimising designs of such complex systems.

Abstract: Porous materials and methods for forming them are disclosed. One method for immobilizing micro-particles and/or nano-particles onto internal pore surfaces and/or external pore surfaces of porous materials includes suspending the micro-particles and/or nano-particles in a liquid adapted to swell, soften, and/or deform either the porous materials and/or the particles, thereby forming a liquid-particle suspension. The method further includes adding the suspension to the porous materials; and removing the liquid, thereby forming the porous materials having the micro-particles and/or nano-particles immobilized on the internal pore surfaces and/or the external pore surfaces.

Abstract: In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.

Abstract: Modified porous materials are disclosed having interconnected, complexly shaped three-dimensional surfaces. The modification is accomplished by crosslinking the three-dimensional surfaces and/or by coating the three-dimensional surfaces with a layer of a predetermined material. The porous materials are macro structures including at least one of nano-features, micro-features, and combinations thereof. The modifying accomplishes changing surface properties of the porous materials, changing the three-dimensional surfaces, and/or rendering the porous materials substantially stable in a predetermined environment.

Abstract: A multiple matchline sense circuit for detecting a single, more than one, or no match conditions during a search-and-compare operation of a content addressable memory is disclosed. The circuit compares the rising voltage rate of a multiple matchline to the rising voltage rate of a reference multiple matchline in order to generate a multibit result representing one of the three conditions. The circuit generates a self-timed control signal to end the search-and-compare operation, and to set the circuit to a precharge state.

Abstract: A matchline sense circuit for detecting a rising voltage on a matchline of a CAM array is disclosed. The circuit initially precharges a matchline to ground before turning on a current source to supply current to the matchline and raise the voltage of the matchline. A reference matchline sense circuit generates a self-timed control signal to keep the current supply turned on for a predetermined duration of time. Sensed data on the matchlines are latched after the current source is turned off and the matchlines are precharged to ground. Because the matchline sense circuit of the present invention precharges the matchlines to ground instead of the supply voltage, VDD, less power is consumed. By sensing the rise of the matchline voltage to an n-channel transistor threshold potential, the matchline sensing operation speed is increased.

Abstract: A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.

Abstract: A bifurcated vascular graft having a tubular trunk with an inner diameter. Tubular limbs having respective inner diameters extend longitudinally from the trunk, and the diameter of at least one of the limbs is unequal to half the trunk diameter. In one embodiment, the graft is flared such that the combined diameters of the limbs exceed the diameter of the trunk. In another embodiment, the graft is tapered such that the diameter of the trunk exceeds the combined diameters of the limbs. In a third embodiment, the limb diameters are unequal to one another.