Abstract: This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Abstract: A method of separating propylene oxide from a mixture (M) comprising propylene oxide and methanol, said method comprising: (i) introducing said mixture (M) into an extractive distillation column; (ii) additionally introducing an extracting solvent into said extractive distillation column; (iii) distilling propylene oxide overhead from said extractive distillation column as top stream; (iv) withdrawing a bottoms stream from said extractive distillation column; (v) compressing the top stream obtained overhead in (iii) by means of at least one compressor to give a compressed vapor.

Abstract: The invention relates to a process for the epoxidation of an olefin comprising (a) reacting the olefin with hydrogen peroxide in the presence of methanol as solvent in at least two reaction stages to obtain a mixture (M-a) comprising olefin oxide, unreacted olefin, methanol and water, wherein between at least two reaction stages, olefin oxide is separated by distillation; (b) separating unreacted olefin from the mixture (M-a) by distillation to obtain a mixture (M-bi) comprising at least 80 wt.-% of olefin and a mixture (M-bii) comprising methanol, water and at least 7 wt.-% of olefin oxide; (c) separating olefin oxide from the mixture (M-bii) in at least one distillation stage to obtain a mixture (M-ci) comprising at least 99 wt.-% of olefin oxide and a mixture (M-cii) comprising water and at least 55 wt.-% of methanol; (d) separating methanol from the mixture (M-cii) in at least one distillation stage to obtain a mixture (M-di) comprising at least 85 wt.-% of methanol and up to 10 wt.

Abstract: The present invention generally relates to optical films useful in the manufacture of polarizer plates, an improved method for producing polarizing plates, and a Liquid Crystal Display employing the same. More particularly, the invention relates to an optical film composite comprising a temporary carrier substrate having a center area and an edge area, said substrate having coated thereon an optical film wherein the adhesive strength between the optical film and the substrate is greater at the edge area than at the center area of the substrate.

Abstract: A method of controlling a spray dryer apparatus having a spray dryer chamber (1), an air inlet (2), an inlet air control system (11) including a fan (13) and an air heater (14), a feed inlet (3), and an exhaust air outlet (4), comprising determining a content (Y) of moisture of inlet air, determining an inlet air temperature set value (Tinlet) in dependence of the inlet air moisture content (Y), determining an inlet air temperature measured value of inlet air between the air heater (14) and the spray dryer chamber (1), and regulating the inlet air temperature in dependence of the inlet air temperature set value and the inlet air temperature measured value, and determining an outlet air temperature of the exhaust air and regulating an inlet air flow rate in dependence of the outlet air temperature.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such asp-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.

Abstract: A method of separating propylene oxide from a mixture (M) comprising propylene oxide and methanol, said method comprising: (i) introducing said mixture (M) into an extractive distillation column; (ii) additionally introducing an extracting solvent into said extractive distillation column; (iii) distilling propylene oxide overhead from said extractive distillation column as top stream; (iv) withdrawing a bottoms stream from said extractive distillation column; (v) compressing the top stream obtained overhead in (iii) by means of at least one compressor to give a compressed vapor.

Abstract: The invention relates to an agglomeration apparatus comprising a fluid bed, a source of descending particles, one or more nozzles for atomizing an agglomeration fluid and an outlet for discharging the agglomerated particles. In the fluid bed, the particles are guided towards the outlet and pass an outer and an inner zone. The apparatus provides for the production of an improved agglomerated particulate product with a low content of fine particles.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: Methods and compositions for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid having a N-acetylgalactosamine moiety into a protein; optionally, the N-acetylgalactosamine-containing unnatural amino acid can be further modified with additional sugars.

Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

Abstract: A method of separating propylene oxide from a mixture (M) comprising 5 to 50 percent by weight propylene oxide and 50 to 85 percent by weight methanol, said method comprising (i) introducing said mixture (M) into an extractive distillation column; (ii) additionally introducing a polar solvent into said extractive distillation column; (iii) distilling propylene oxide overhead from said extractive distillation column at a bottoms temperature of from 40 to 70° C. and at a pressure of from 300 to 750 mbar.