Abstract: It is a main object of the present invention to provide a process for producing a neuron-like cell from a somatic cell, a neuron-like cell obtained thereby, and a composition comprising a combination of chemical substances that can be used for said process without performing artificial gene transfer. The invention can include, for example, a process for producing neuron-like cells, characterized by comprising a step of culturing somatic cells in the presence of two kinds of inhibitors, i.e., a TGF-? inhibitor and a BMP inhibitor, as well as any three or more selected from the group consisting of four kinds, i.e., a cAMP inducer, a GSK3 inhibitor, an Erk-inhibitor, and a p53-inhibitor, wherein the TGF-? inhibitor is a selective ALK5 inhibitor, and a neuron-like cell obtained by said process. The neuron-like cells obtained according to the present invention are useful in regenerative medicine and the like.

Abstract: The present invention chiefly aims to provide a process for directly inducing insulin-producing cells from somatic cells without performing artificial gene transfer. The present invention can include a process for producing an insulin-producing cell by inducing differentiation directly from a somatic cell, the process comprising a step of culturing the somatic cell in the presence of a cAMP inducer, and six members selected from the group consisting of a GSK3 inhibitor, a TGF-? inhibitor, a BMP inhibitor, a p53 inhibitor, a PI3K inhibitor, a Notch inhibitor and a RAR agonist, or all members thereof. The insulin-producing cells obtained by the present invention are useful in regenerative medicine and the like.

Abstract: Disclosed herein are methods, systems, and apparatus, including computer programs encoded on computer storage media, for blockchain transaction processing. One of the methods includes: obtaining, by a blockchain node, a current blockchain transaction generated based on performing a service; obtaining, by the blockchain node based on executing a smart contract, a transaction identifier of a previous blockchain transaction; recording, by the blockchain node, the transaction identifier into a transaction log so that a previous blockchain transaction is identifiable from the transaction log based on the transaction identifier, wherein the transaction log stores blockchain transactions of the current block; and recording the blockchain transaction of the current block and the transaction log into the blockchain.

Abstract: Disclosed herein are methods, systems, and apparatus, including computer programs encoded on computer storage media, for processing blockchain transactions. One of the methods includes: obtaining, by a blockchain node of a consortium blockchain network, a blockchain transaction associated with a service; invoking a smart contract corresponding to the service; determining presentation information based on the smart contract and the service, wherein the presentation information is presented to a user through a client device in response to receiving a query from the client device; recording the presentation information into a blockchain transaction log based on the smart contract; and recording the blockchain transaction and the blockchain transaction log of the blockchain transaction into a blockchain.

Abstract: The present invention chiefly aims to provide a process for directly inducing cardiomyocytes from somatic cells without performing artificial gene transfer, a cardiomyocyte obtained thereby, and a composition comprising a combination of chemical compounds capable of using for the said process. The present invention can include a process for producing a cardiomyocyte by inducing differentiation directly from a somatic cell, the process comprising a step of culturing the somatic cell in the presence of a MEK inhibitor and a cAMP inducer, and a cardiomyocyte obtained thereby, and then a composition for producing a cardiomyocyte by inducing differentiation directly from a somatic cell, the composition comprising a MEK inhibitor and a cAMP inducer. The cardiomyocytes obtained according to the present invention are useful in regenerative medicine and the like.

Abstract: Disclosed herein are methods, systems, and apparatus, including computer programs encoded on computer storage media, for blockchain transaction querying. One of the methods includes: detecting, by a server of a consortium blockchain network, that a blockchain transaction query interface is invoked by a client device of the consortium blockchain network to query a blockchain transaction; obtaining, by the server, the blockchain transaction corresponding to the blockchain transaction query interface from the consortium blockchain network; and sending, by the server, the blockchain transaction to the client device.

Abstract: Disclosed herein are methods, systems, and apparatus, including computer programs encoded on computer storage media, for blockchain transaction processing. One of the methods includes: obtaining, by a blockchain node, a current blockchain transaction generated based on performing a service; obtaining, by the blockchain node based on executing a smart contract, a transaction identifier of a previous blockchain transaction; recording, by the blockchain node, the transaction identifier into a transaction log so that a previous blockchain transaction is identifiable from the transaction log based on the transaction identifier, wherein the transaction log stores blockchain transactions of the current block; and recording the blockchain transaction of the current block and the transaction log into the blockchain.

Abstract: Disclosed herein are methods, systems, and apparatus, including computer programs encoded on computer storage media, for processing blockchain transactions. One of the methods includes: obtaining, by a blockchain node of a consortium blockchain network, a blockchain transaction associated with a service; invoking a smart contract corresponding to the service; determining presentation information based on the smart contract and the service, wherein the presentation information is presented to a user through a client device in response to receiving a query from the client device; recording the presentation information into a blockchain transaction log based on the smart contract; and recording the blockchain transaction and the blockchain transaction log of the blockchain transaction into a blockchain.

Abstract: The present invention addresses the problem of providing: a method for producing brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells from somatic cells without performing artificial gene transfer; brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells; or a composition including a combination of chemical substances that can be used for the aforementioned production method. An example of the present invention is a method for producing brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells including a step for culturing somatic cells in the presence or absence of an inhibitor or activator selected from the group consisting of an ALK5 inhibitor, an ALK6 inhibitor, an AMPK inhibitor, a cAMP activator, an ALK2 inhibitor, an ALK3 inhibitor, a GSK3 inhibitor, and an Erk inhibitor.

Abstract: Example data processing methods and systems are provided. A server obtains first order data submitted by a client. The first order data includes user address information. A matching operation is performed according to the user address information in a preset database to find second order data that satisfies an address matching condition. A merging operation is applied to merge second order data that satisfies the address matching condition with the first order data from the client.

Abstract: A request is received to migrate a virtual instance from a source host compute node. The source host compute node is included in a distributed storage system environment. The virtual instance is migrated from the source host compute node to a first target host compute node. The first target host compute node has access to a first storage device. The first storage device includes one or more data units associated with the virtual instance. As part of the request to migrate the virtual instance, the one or more data units are migrated to a second storage device associated with the first target host compute node.

Abstract: A request is received to migrate a virtual instance from a source host compute node. The source host compute node is included in a distributed storage system environment. The virtual instance is migrated from the source host compute node to a first target host compute node. The first target host compute node has access to a first storage device. The first storage device includes one or more data units associated with the virtual instance. As part of the request to migrate the virtual instance, the one or more data units are migrated to a second storage device associated with the first target host compute node.

Abstract: Provided herein are formulations comprising antibodies that specifically bind to Programmed Death-1 (PD-1) and methods of making such formulations.

Abstract: The present invention provides a method with which it is possible to directly induce nervous system cells efficiently and in a short amount of time. Because the method is easy to scale up and is not affected by the characteristics or background of the somatic cells used as material, the method enables the stable supply of nervous system cells. The nervous system cells obtained by the method are useful in various fields of research and healthcare.

Abstract: The present invention describes methods and processes for the production of proteins by animal cell or mammalian cell culture. In one aspect, the methods comprise the growth of cells in a growth factor/protein/peptide free medium. In another aspect, the methods comprise the addition of growth factors during the production phase. The methods sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product.

Abstract: A carrier service server, in response to determining that signals of a called user cannot be searched through a base station, queries a user ID bound to the called number of the called user to send a message indicating that there is an incoming call or a short message to a client terminal corresponding to the user ID via the Internet. The client terminal executes an operation that enables the called user to receive a call or short message content according to a preset configuration. Thus, the called user may, when a communication module for phone signals is closed or there is no mobile communication signal, timely receive the message through the client terminal connected with the Internet.

Abstract: Example data processing methods and systems are provided. A server obtains first order data submitted by a client. The first order data includes user address information. A matching operation is performed according to the user address information in a preset database to find second order data that satisfies an address matching condition. A merging operation is applied to merge second order data that satisfies the address matching condition with the first order data from the client.

Abstract: The present invention provides a method for producing iPS cells, comprising reacting cells with at least one connexin inhibitor and at least one TGF? signaling inhibitor; iPS cells comprising at least one connexin inhibitor; an iPS cell inducer comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; a medium for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; and a kit for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors.

Abstract: The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.

Abstract: In a method for controlling an alarm function of an electronic device, an alarm time, an alarm mode, a plurality of control modes of the alarm function and a shaking frequency and an audio file corresponding to each of the control modes are set. If a current time matches the alarm time, the alarm function is started by activating the alarm mode and a monitor unit is enabled to monitor acceleration values of the electronic device. A first shaking frequency in a first predetermined time limit is recorded according to the acceleration values. The control mode corresponding to the first shaking frequency is confirmed, and the audio file corresponding to the confirmed control mode is output. The method further controls the alarm function according to the confirmed control mode.