Abstract: The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.

Abstract: The present invention describes methods and processes for the production of proteins by animal cell or mammalian cell culture. In one aspect, the methods comprise the growth of cells in a growth factor/protein/peptide free medium. In another aspect, the methods comprise the addition of growth factors during the production phase. The methods sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product.

Abstract: Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.

Abstract: In a method of transmitting a file of an electronic device, the electronic device sets one or more borderlines of a touch screen, and generates a search signal to search a recipient electronic device to receive the file when the file has been dragged outside of one of the borderlines.

Abstract: Disclosed herein is a flooring underlayment membrane, and associated methods of manufacturing and installing such a membrane, comprising upper and lower nonwoven fiber layers. In one embodiment, the flooring underlayment membrane constructed as disclosed herein may comprise a first nonwoven fiber material, and a second nonwoven fiber material and at least one layer of extrudable thermoplastic resin disposed between and bonding the first and second nonwoven fiber materials.

Abstract: In a method for controlling an alarm function of an electronic device, an alarm time, an alarm mode, a plurality of control modes of the alarm function and a shaking frequency and an audio file corresponding to each of the control modes are set. If a current time matches the alarm time, the alarm function is started by activating the alarm mode and a monitor unit is enabled to monitor acceleration values of the electronic device. A first shaking frequency in a first predetermined time limit is recorded according to the acceleration values. The control mode corresponding to the first shaking frequency is confirmed, and the audio file corresponding to the confirmed control mode is output. The method further controls the alarm function according to the confirmed control mode.

Abstract: Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.

Abstract: The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.

Abstract: The present invention provides a method for producing iPS cells, comprising reacting cells with at least one connexin inhibitor and at least one TGF? signaling inhibitor; iPS cells comprising at least one connexin inhibitor; an iPS cell inducer comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; a medium for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; and a kit for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors.

Abstract: According to one aspect, an optical read/write device for reading and/or writing an optical disc includes a base, optical pickup head, and a driving mechanism for slidably driving the optical pickup head. The driving mechanism includes a worm gear, a motor for rotating the worm gear, and a rack disposed between the worm gear and the optical pickup head for transferring motion from the worm gear to the optical pickup head. The rack includes a fastening portion for fixing the rack to the optical pickup head, a mesh portion for engaging with the worm gear, a restricting portion connected to the mesh portion for being blocked by the gear seat when the mesh portion is pressed by the worm gear to prevent the mesh portion from disengaging from the worm gear, and a connecting portion for connecting the fastening portion with the restricting portion.

Abstract: An airflow guider includes a plate-like member having a stop portion extending transversely, a rear inclined guiding portion extending downward rearward toward a first side from a top edge of the stop portion, a front inclined guiding portion extending slantwise forward toward the first side from the stop portion, and at least one fixture for installing the airflow guider to a heat sink. In light of the structure, the airflow guider can be coordinately installed to various kinds of heat sinks and provide additional thermal dissipation.

Abstract: A portable electronic device includes a main body and a subsidiary casing. The main body includes a first side wall and a bottom wall. The first side wall defines at least one receptacle and at least one hole therein. The receptacle includes a cavity and a slot. The cavity spans from the bottom wall up into the main body. The slot is defined vertically in a part of the first side wall. The slot intercommunicates between the cavity and an exterior of the first side wall. The subsidiary casing includes a base and a cover. At least one latch and at least one connector are formed on the base. The latch is configured for being received in the receptacle and the connector is configured for being received in the hole.

Abstract: An airflow guider includes a plate-like member having a stop portion extending transversely, a rear inclined guiding portion extending downward rearward toward a first side from a top edge of the stop portion, a front inclined guiding portion extending slantwise forward toward the first side from the stop portion, and at least one fixture for installing the airflow guider to a heat sink. In light of the structure, the airflow guider can be coordinately installed to various kinds of heat sinks and provide additional thermal dissipation.

Abstract: Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.

Abstract: A heat-dissipating fin includes a sheety main body. The main body is provided with a high-temperature area located at each of two sides thereof, an airflow area located at a midsection thereof for an external airflow to pass through, at least one guide wall formed at the airflow area and having a front end facing the main body, and two inclined guide portions each extending rearward toward one side thereof from a front end thereof. In this way, the external airflow can be guided to the high-temperature areas of the fin to reach greater heat-dissipating efficiency.

Abstract: Disclosed herein is a flooring underlayment membrane, and associated methods of manufacturing and installing such a membrane, comprising upper and lower nonwoven fiber layers. In one embodiment, the flooring underlayment membrane constructed as disclosed herein may comprise a first nonwoven fiber material, and a second nonwoven fiber material and at least one layer of extrudable thermoplastic resin disposed between and bonding the first and second nonwoven fiber materials.

Abstract: The present invention relates to a treatment agent for a disease or a disorder caused by a reduction in corneal endothelial cells, comprising as an active component at least one nucleic acid molecule inhibiting the expression of a connexin 43 gene.

Abstract: According to one aspect, an optical read/write device for reading and/or writing an optical disc includes a base, optical pickup head, and a driving mechanism for slidably driving the optical pickup head. The driving mechanism includes a worm gear, a motor for rotating the worm gear, and a rack disposed between the worm gear and the optical pickup head for transferring motion from the worm gear to the optical pickup head. The rack includes a fastening portion for fixing the rack to the optical pickup head, a mesh portion for engaging with the worm gear, a restricting portion connected to the mesh portion for being blocked by the gear seat when the mesh portion is pressed by the worm gear to prevent the mesh portion from disengaging from the worm gear, and a connecting portion for connecting the fastening portion with the restricting portion.

Abstract: An opening actuator for a video disc player includes a clasp arm with a clasp hole at a distal end thereof, a press button, an elastic component for repositioning the press button and a cover. The press button includes a stop portion for limiting a stroke of the press button, a clasp portion, and at least one guide portion. The cover includes a positioning member and at least one guide member receiving the at least one guide portion. The clasp portion of the press button engages in the clasp hole of the clasp arm for holding the top lid on the cover, and the press button can actuate the clasp portion thereof to exit the clasp hole for releasing the top lid from the cover. Because the press button is located in a side of the cover of the video disc player, there is little risk of the press button being accidentally actuated.

Abstract: A portable electronic device includes a main body and a subsidiary casing. The main body includes a first side wall and a bottom wall. The first side wall defines at least one receptacle and at least one hole therein. The receptacle includes a cavity and a slot. The cavity spans from the bottom wall up into the main body. The slot is defined vertically in a part of the first side wall. The slot intercommunicates between the cavity and an exterior of the first side wall. The subsidiary casing includes a base and a cover. At least one latch and at least one connector are formed on the base. The latch is configured for being received in the receptacle and the connector is configured for being received in the hole.