Abstract: The invention relates to integrated DNA and RNA library preparations and methods of making and uses thereof, wherein the DNA molecules are labelled with a tag identifying the molecule as being a DNA molecule and the RNA molecules are labelled with a tag identifying the molecule as being a RNA molecule. The methods do not require physical separation of DNA and RNA. The methods output two separate libraries from DNA and RNA, respectively, which helps flexible manipulation on downstream sequencing platforms. The application also claims the DNA library and the cDNA library produced by the method and the DNA tag and the RNA tag used in the method.

Abstract: Disclosed herein are methods for producing DNA libraries by incorporating dUTPs into DNA fragments and treating with uracil-DNA glycosylase and kits for preparing the DNA libraries. The DNA libraries are advantageous for next generation sequencing.

Abstract: The present invention provides a porous tricalcium phosphate material, modified from a coral bone and having a plurality of pores, wherein the average compressive strength of the porous tricalcium phosphate material is 4 kgf to 9 kgf. Besides, the present invention also provides a method for bone healing and a manufacturing method for the porous tricalcium phosphate material. The porous tricalcium phosphate material of the present invention has the advantages of biocompatibility and no immunological rejection response, and also has significantly better mechanical properties and bone healing efficacy so as to better meet the needs of the patients.

Abstract: An electronic device and a method for detecting an intensity of ambient light are provided. The method includes: sensing a light intensity of incident light, wherein the incident light includes ambient light passed through the display screen and light leaked from the display screen; obtaining an estimated light intensity of the light leaked from the display screen based on display data of a target region of the display screen; computing based on the estimated light intensity and a temperature compensation coefficient corresponding to a current temperature of the display screen, to obtain a light intensity of the light leaked from the display screen; and obtaining a light intensity of the ambient light passed through the display screen based on the light intensity of the incident light and the light intensity of the light leaked from the display screen.

Abstract: The present disclosure relates to the field of tungsten smelting, and in particular to a leaching method of scheelite, and provides a new technology that scheelite is leached in low temperature with a mixture acid of sulfuric acid and hydrochloric acid or nitric acid and hydrochloric acid, calcium is precipitated incompletely, and then the acid leaching solution is regenerated and closed-circuit recycled is provided. The new technology is able to leach scheelite sufficiently in low temperature so as to improve the recovery rate of tungstate and decrease the requirement of equipment. As well, the inner wall of the reaction kettle, the heating pipe and the temperature measuring device are made of steel lined tetrafluoro material which has high acid corrosion resistance and is able to deal with scheelite with high fluorine, resists the corrosion of HF.

Abstract: An electronic device and a method for detecting an intensity of ambient light are provided. The method includes: sensing a light intensity of incident light, wherein the incident light includes ambient light passed through the display screen and light leaked from the display screen; obtaining an estimated light intensity of the light leaked from the display screen based on display data of a target region of the display screen; computing based on the estimated light intensity and a temperature compensation coefficient corresponding to a current temperature of the display screen, to obtain a light intensity of the light leaked from the display screen; and obtaining a light intensity of the ambient light passed through the display screen based on the light intensity of the incident light and the light intensity of the light leaked from the display screen.

Abstract: The invention relates to methods of enriching for target nucleic acid molecules, More particularly, the methods of enriching for target nucleic acid molecules comprise binding target nucleic acid molecules in a sample with one or more first target endonucleases that are specific to a first locus of a target region of the target nucleic acid molecules, separating the target nucleic acid molecules from nontarget nucleic acid molecules in the sample, and binding the separated target nucleic acid molecules with one or more second target endonucleases that are specific to a second locus of the target region of the target nucleic acid molecules, and uses thereof.

Abstract: The present invention provides a porous tricalcium phosphate material, modified from a coral bone and having a plurality of pores, wherein the average compressive strength of the porous tricalcium phosphate material is 4 kgf to 9 kgf. Besides, the present invention also provides a method for bone healing and a manufacturing method for the porous tricalcium phosphate material. The porous tricalcium phosphate material of the present invention has the advantages of biocompatibility and no immunological rejection response, and also has significantly better mechanical properties and bone healing efficacy so as to better meet the needs of the patients.

Abstract: The present disclosure provides primers, primer sets, kits and methods for multiple displacement amplification, especially in combination with nucleic acid sequencing. The primers comprise self-complementary sequences at their 5? termini and random or semi-random sequences at their 3? termini. Use of such primers facilitates handling of multiple samples, increases sequence coverage uniformity, and improves sequencing error corrections.

Abstract: The present disclosure provides primers, primer sets, kits and methods for multiple displacement amplification, especially in combination with nucleic acid sequencing. The primers comprise self-complementary sequences at their 5? termini and random or semi-random sequences at their 3? termini. Use of such primers facilitates handling of multiple samples, increases sequence coverage uniformity, and improves sequencing error corrections.

Abstract: The invention relates to methods of detecting analytes in samples by generating analyte-based DNA libraries amenable for sequencing. The methods include the use of proximity probe pairs, each probe comprising an analyte binding domain and oligonucleotide domain. The methods further provide for integrated DNA and RNA library preparations and methods of making and uses thereof. The invention also provides compositions useful in the methods.

Abstract: The present disclosure provides methods and kits for highly multiplex single primer extensions using a MutS protein and Mg2+ at a concentration higher than that in a typical PCR reaction. Also disclosed is the use of such methods and kits in next generation sequencing.

Abstract: A cathode of a lithium-air battery includes a carbon nanotube composite film and a protecting layer. The carbon nanotube composite film includes a carbon nanotube network structure and a catalyst in particle form located in the carbon nanotube network structure. The carbon nanotube composite film is disposed on a surface of the protecting layer. The protecting layer allows conduction of lithium ions while preventing organic substances in an electrolyte of the lithium-air battery reaching the carbon nanotube composite film. A lithium-air battery is also disclosed.

Abstract: The present disclosure provides methods and kits for highly multiplex single primer extensions using a MutS protein and Mg2+ at a concentration higher than that in a typical PCR reaction. Also disclosed is the use of such methods and kits in next generation sequencing.

Abstract: The invention relates to integrated DNA and RNA library preparations and methods of making and uses thereof, wherein the DNA molecules are labelled with a tag identifying the molecule as being a DNA molecule and the RNA molecules are labelled with a tag identifying the molecule as being a RNA molecule. The methods do not require physical separation of DNA and RNA. The methods output two separate libraries from DNA and RNA, respectively, which helps flexible manipulation on downstream sequencing platforms. The application also claims the DNA library and the cDNA library produced by the method and the DNA tag and the RNA tag used in the method.

Abstract: The present disclosure provides methods and kits for performing high multiplex PCR using molecular barcodes. The methods disclosed herein separately extend a set of primers (BC primers) that each comprise a molecular barcode and a target-specific sequence, and amplify the resulting extension products using another set of primers (LA primers) that each comprise another target-specific sequence. Such methods avoid barcode resampling and suppress primer dimers in traditional high multiplex PCR.

Abstract: The present disclosure provides methods and kits for performing high multiplex PCR using molecular barcodes. The methods disclosed herein separately extend a set of primers (BC primers) that each comprise a target-specific sequence, a molecular barcode and a universal sequence, and amplify the resulting extension products using another set of primers (LA primers) that each comprise another target-specific sequence and a universal sequence. The methods may further comprise amplification using universal primers (preferably comprising an adapter).

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.