Abstract: The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.

Abstract: A gas sensor is for determining at least one physical quantity of a gas, e.g., an exhaust gas of an internal combustion engine, the gas sensor having a sensor element that is fixed in a housing of the gas sensor by a seal assembly. The seal assembly includes a sealing element, which has a ceramic and/or a metallic material. After the heat treatment, the sealing element has a maximum decrease in volume of 5 percent, or an increase in volume, based on the volume of the sealing element prior to the heat treatment.

Abstract: A communication system, network, interface, and port architecture are provided for transporting different types of data across a network. The network can be arranged by connecting the ports in a daisy-chain fashion to achieve a ring architecture or topology. The network forwards data according to a specific network protocol, and nodes among the network can use a recovered clock from the bitstream to drive the transmitter and create a synchronous network even thought the network can receive Ethernet packets which are typically targeted for an asynchronous network. Non-compliant data, such as Ethernet data, can be sent as packets within a frame structure. The frame is synchronized to Ethernet coding violations used to signal the beginning of each frame, and each packet can be immediately followed by a packet coding violation. The frame and packet coding violations are represented as a sequence of 4B/5B codes that are not valid codes and, therefore, not present as Ethernet encoded data.

Abstract: A gas sensor is for determining at least one physical quantity of a gas, e.g., an exhaust gas of an internal combustion engine, the gas sensor having a sensor element that is fixed in a housing of the gas sensor by a seal assembly. The seal assembly includes a sealing element, which has a ceramic and/or a metallic material. After the heat treatment, the sealing element has a maximum decrease in volume of 5 percent, or an increase in volume, based on the volume of the sealing element prior to the heat treatment.

Abstract: The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.

Abstract: The invention concerns recombinant proteinase K. Furthermore a method for producing recombinant proteinase K is disclosed, which is characterized in that a) a host cell is transformed with a recombinant nucleic acid which codes for the zymogenic precursor of proteinase K, b) the host cell is cultured in such a manner that the zymogenic precursor of proteinase K is formed in the form of inclusion bodies in the host cell, c) the inclusion bodies are isolated and natured under conditions which result in the formation of the protease part of the zymogenic precursor in its natural conformation, d) the natured proteinase K is activated and purified.

Abstract: The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.

Abstract: A casing assembly for a turbocharger of an internal combustion engine includes a rotor casing having an intake duct and an exhaust flow passage which is connected to an exhaust flange and constructed for attachment to the rotor casing such as to allow a relative movement between the exhaust flow passage and the rotor casing. In this way, a sliding fit is provided which prevents thermal stress in the system rotor casing and exhaust flow passage. An outer housing surrounds the exhaust flow passage, rotor casing and intake duct.

Abstract: The invention concerns novel hair cleansing and conditioning compositions comprising, in a cosmetically suitable medium, (A) a washing base and (B) at least a C4–C6 carboxylic acid ester and a C12–C26 alcohol. The invention is useful for hair wash and hair care.

Abstract: A method is provided for the production of a bovine pancreatic protein with a specific desoxyribonuclease activity of at least 6,000 units per mg of protein. Methylotrophic yeast is used as a heterologous host organism. The bovine pancreatic protein is secreted into the growth medium from which it is purified.

Abstract: By deglycosylating an enzyme derived from a eukaryotic organism prior to forming a conjugate, i.e. prior to attaching the enzyme to a molecule capable of binding to a target molecule, the sensitivity of an assay for detecting the presence or determining the quantity of a target molecule, in which the conjugate is used as a labelled component, can be increased. The present invention provides conjugates, methods for providing the same, use of such conjugates as well as kits containing the conjugates.

Abstract: An electric motor, in particular an external rotor motor, comprising a stator which is attached by means of latching elements to a motor carrier using a plug-in joining operation. The elastic elements for isolating vibration are arranged in the connecting region between the stator and the motor carrier in a way that the stator is indirectly connected to the motor carrier by means of the elastic elements.

Abstract: The invention provides a method to produce a protein with carboxypeptidase B activity from a pro-carboxypeptidase B zymogen, derived from a non-animal host organism. Carboxypeptidase B is activated from the zymogen using non-denaturing conditions. Particularly, the activation is performed under conditions that avoid unwanted non-covalent binding of the propeptide to the activated carboxypeptidase B enzyme.

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.

Abstract: This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.

Abstract: An insulation arrangement includes an insulation packet that is made up of insulation material completely encased by a film, and that is arranged in an interspace between an inner trim component and an outer skin of an aircraft. The insulation packet does not completely fill the interspace, but rather leaves an air space. The film consists of a differentially gas permeable film material that provides a differentiated diffusion resistance with respect to water vapor diffusing therethrough, dependent on the direction of the diffusion through the film. Preferably the resistance to diffusion of water vapor into the insulation packet through the film is higher than the resistance to diffusion of water vapor out of the insulation packet through the film. The accumulation of moisture in the packet is avoided.

Abstract: Adhesion promoters for polymer coatings on metals are articulated into at least two head groups connected by a hydrocarbon spacer group. One head group reacts particularly well with polymer molecules. Other head groups form a durable bond with a metal surface. The length of the spacer group is such that a substance deposited in a substance layer on a metallic surface of a body or particle has hydrophobic properties. Suitable adhesion promoters can be sulfur compounds, amino compounds, hydroxyl or carbonyl compounds. A plastic composite body, a compound and a process of preparing a compound are also provided.

Abstract: The invention concerns a method for the expression of a gene coding for a soluble proteinase K in yeast e.g. in Pichia pastoris with subsequent secretion into the culture medium. In addition a method for purifying the heterologously expressed and secreted proteinase K is described.

Abstract: Process and device for treating seeds in order to combat harmful organisms. According to the invention, in an initial stage of the process, the seeds are separated and distributed evenly in a free-fall process, subjected in a vacuum to irradiation by low energy electron beams and then immediately treated with fungicides and/or microbial antagonists or metabolic products and spores thereof and/or synergists.

Abstract: The invention concerns novel hair cleansing and conditioning compositions comprising, in a cosmetically suitable medium, (A) a washing base and (B) at least a C12-C26 carboxylic acid ester and a C12-C26 alcohol. The invention is useful for hair wash and hair care.