Patents by Inventor Ralf Lenigk
Ralf Lenigk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180327811Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific binding element. This results in the protamine-specific binding element binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: ApplicationFiled: July 23, 2018Publication date: November 15, 2018Inventors: Patrick McCoy SPOONER, Peter James TATNELL, Jeffrey Kenneth HORTON, John Richard NELSON, Michael John GERDES, Suzana KIEL, Ralf LENIGK, Alexander SCHENK, Wei SUN, Thomas HANSEN
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Publication number: 20180257077Abstract: Instruments, devices and methods of analysis are provided which fully integrate a significant number of process steps in a continuous operation. Accurate positioning and full contact between components is also provided by the relative movement the designs allow. An effect interface between a low cost disposable cartridge or device and the instrument to process it is also detailed.Type: ApplicationFiled: January 10, 2018Publication date: September 13, 2018Inventors: Frederic ZENHAUSERN, Alan NORDQUIST, Ralf LENIGK, Cedric HURTH, Jianing YANG, Xiaojia CHEN, John LEE-EDGHILL, Nina MORAN, Andrew HOPWOOD, Pieris KOUMI
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Patent number: 10033910Abstract: The present approach relates to the synchronization of frame acquisition by a camera with an external event or trigger despite the camera lacking external control or synchronization capabilities. For example, inexpensive and/or consumer grade camera typically lack a control interface to explicitly synchronize with an external trigger event or external device. The present approach allows synchronization of such a camera lacking external synchronization capabilities with an external event or device.Type: GrantFiled: April 15, 2016Date of Patent: July 24, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Ralf Lenigk, Mark Marshall Meyers, Victor Petrovich Ostroverkhov, Timothy Toepfer, Keith Michael Looney
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Patent number: 10030241Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: GrantFiled: May 10, 2016Date of Patent: July 24, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Patrick McCoy Spooner, Peter James Tatnell, Jeffrey Kenneth Horton, John Richard Nelson, Michael John Gerdes, Suzana Kiel, Ralf Lenigk, Alexander Schenk, Wei Sun
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Publication number: 20180185843Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface; and an outlet for driving out the sample fluid. The device further comprises a third layer comprising an adhesive material such as a patterned adhesive material and a flow path, wherein the third layer is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer and the outlet is in contact with the flow path of the third layer for allowing the sample fluid out from the integrated device.Type: ApplicationFiled: February 26, 2018Publication date: July 5, 2018Inventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Patent number: 9999856Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: GrantFiled: July 26, 2013Date of Patent: June 19, 2018Assignee: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Patent number: 9957548Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.Type: GrantFiled: March 30, 2015Date of Patent: May 1, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Michael John Gerdes, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Patent number: 9901922Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface; and an outlet for driving out the sample fluid. The device further comprises a third layer comprising an adhesive material such as a patterned adhesive material, and a flow path, wherein the third layer is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer and the outlet is in contact with the flow path of the third layer for allowing the sample fluid out from the integrated device.Type: GrantFiled: July 25, 2014Date of Patent: February 27, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Publication number: 20170363561Abstract: The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.Type: ApplicationFiled: December 3, 2015Publication date: December 21, 2017Inventors: Andrea Jeannine Peters, Thomas Elliot Stecher, Craig Patrick Galligan, Christopher Michael Puleo, Ralf Lenigk
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Publication number: 20170302826Abstract: The present approach relates to the synchronization of frame acquisition by a camera with an external event or trigger despite the camera lacking external control or synchronization capabilities. For example, inexpensive and/or consumer grade camera typically lack a control interface to explicitly synchronize with an external trigger event or external device. The present approach allows synchronization of such a camera lacking external synchronization capabilities with an external event or device.Type: ApplicationFiled: April 15, 2016Publication date: October 19, 2017Inventors: Ralf Lenigk, Mark Marshall Meyers, Victor Petrovich Ostroverkhov, Timothy Toepfer, Keith Michael Looney
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Publication number: 20170211982Abstract: Disclosed is a low-cost, portable photo thermal spectroscopy (PTS) reader for use in detecting the presence of diseases in the bodily fluid of affected patients. The PTS reader is designed to be durable, easy to use and provide readings from the Lateral Flow Assay (LFA) with rapid results. Also provided are methods of use.Type: ApplicationFiled: July 22, 2016Publication date: July 27, 2017Inventors: Ralf Lenigk, Mark Marshall Meyers, Victor Petrovich Ostroverkhov, Timothy Toepfer, Keith Michael Looney, Terry Lee Saunders, Kevin Paul Flood Nichols, Matthew P. Horning, Benjamin K. Wilson, Daniel Keith Connors, David Gasperino
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Patent number: 9714447Abstract: The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.Type: GrantFiled: August 19, 2013Date of Patent: July 25, 2017Assignee: General Electric CompanyInventors: Erin Jean Finehout, Christopher Michael Puleo, Ralf Lenigk, Jason M. Nichols, John Nelson
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Publication number: 20170203292Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.Type: ApplicationFiled: July 20, 2015Publication date: July 20, 2017Applicant: General Electric CompanyInventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Publication number: 20170153223Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.Type: ApplicationFiled: December 1, 2015Publication date: June 1, 2017Inventors: Jessica Godin Karp, Xiaohui Chen, Kashan Ali Shaikh, Mengli Wang, Ralf Lenigk, Christine Lynne Surrette
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Publication number: 20170087553Abstract: A method and device structure are provided which enable an archive sample to be collected and detached relative to a device within which a series of processes, such as PCR are being provided. A chamber structure and method of use are provided in which a controlled and precise volume is obtained by control of the relative resistance to flow through various channels.Type: ApplicationFiled: September 2, 2016Publication date: March 30, 2017Inventors: Frederic ZENHAUSERN, Alan NORDQUIST, Ralf LENIGK, Cedric HURTH, Jianing YANG, Xiaojia CHEN, Matthew ESTES, John LEE-EDGHILL, Nina MORAN, Andrew HOPWOOD, Pieris KOUMI, Zhi CAI
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Patent number: 9480981Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.Type: GrantFiled: July 25, 2014Date of Patent: November 1, 2016Assignee: General Electric CompanyInventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Publication number: 20160289735Abstract: A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.Type: ApplicationFiled: March 30, 2015Publication date: October 6, 2016Inventors: Michael John Gerdes, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Publication number: 20160289666Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: ApplicationFiled: May 10, 2016Publication date: October 6, 2016Inventors: Patrick McCoy Spooner, Peter James Tatnell, Jeffrey Kenneth Horton, John Richard Nelson, Michael John Gerdes, Suzana Kiel, Ralf Lenigk, Alexander Schenk, Wei Sun
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Publication number: 20160243542Abstract: An apparatus for processing a sample, circuit board suited to use in such an apparatus and a method of processing in which the apparatus has alignment features to assist in aligning the circuit board and a sample cartridge in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge are positioned against a plurality of elements, such as a heater for heating the sample cartridge or an electrical contact (EP1-EP4) for powering features on the sample cartridge, correctly and can operate independently of one another.Type: ApplicationFiled: September 18, 2015Publication date: August 25, 2016Inventors: Stan SMITH, Brett DUANE, Cedric HURTH, David NGUYEN, Amol SURVE, Ralf LENIGK, Frederic ZENHAUSERN
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Publication number: 20160187293Abstract: An electrophoresis device where the end of the electrophoresis channel has a part that extends vertically. The electrophoresis channel includes a first access port at one end of the channel and a second port at the other end of the channel, which are in the same horizontal plane. In this way, there is a less than 0.1% hydrostatic pressure differential between two ports and the performance of the device is improved.Type: ApplicationFiled: June 11, 2012Publication date: June 30, 2016Applicants: The Arizona Board of Regents acting for and on behalf of University of Arizona, BioAccelInventors: Frederic ZENHAUSERN, Alan NORDQUIST, Ralf LENIGK, Cedric HURTH, Jianing YANG, Xiaojia CHEN, Matthew ESTES, John LEE-EDGHILL, Nina MORAN, Andrew HOPWOOD, Pieris KOUMI