Patents by Inventor Ralf Lenigk
Ralf Lenigk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160168623Abstract: A method of analysing a sample includes providing a first part of the sample and a second part of the sample. A first analysis is conducted on the first part of the sample and the results of the first analysis are considered. A second analysis is conducted on the second part of the sample, the second analysis being conducted according to a procedure using a value for each of one or more characteristics of the procedure. The consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value. The second analysis is started before the results of the first analysis are obtained.Type: ApplicationFiled: July 15, 2015Publication date: June 16, 2016Inventors: Frederic ZENHAUSERN, Alan NORDQUIST, Ralf LENIGK, Cedric HURTH, Andrew HOPWOOD, Pieris KOUMI, Gill TULLY
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Publication number: 20160161437Abstract: The present disclosure relates to the manufacture and use of redox electrodes. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 V.Type: ApplicationFiled: December 3, 2014Publication date: June 9, 2016Inventors: Andrea Jeannine Peters, Thomas Elliot Stecher, Craig Patrick Galligan, Christopher Michael Puleo, Ralf Lenigk
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Patent number: 9333463Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.Type: GrantFiled: July 26, 2013Date of Patent: May 10, 2016Assignee: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Patent number: 9255348Abstract: Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures.Type: GrantFiled: August 24, 2007Date of Patent: February 9, 2016Assignee: The Trustees of Columbia University in the City of New YorkInventors: Frederic Zenhausern, Christine Orozco, Mark Richards, Carl Yamashiro, Sally A. Amundson, Ralf Lenigk, Michael L. Bittner, Yoganand Balagurunathan
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Publication number: 20160023209Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface; and an outlet for driving out the sample fluid. The device further comprises a third layer comprising an adhesive material such as a patterned adhesive material, and a flow path, wherein the third layer is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer and the outlet is in contact with the flow path of the third layer for allowing the sample fluid out from the integrated device.Type: ApplicationFiled: July 25, 2014Publication date: January 28, 2016Inventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Publication number: 20160023206Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.Type: ApplicationFiled: July 25, 2014Publication date: January 28, 2016Inventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
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Patent number: 9199201Abstract: A pump comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes; and a plurality of electrodes comprising one or more cathodes and one or more anodes. The electrodes are pre-charged, chargeable, rechargeable or combinations thereof and the cathode and anode are operatively coupled to each other. The positive electroosmotic membranes and negative electroosmotic membranes are disposed alternatively and at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane, and wherein at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane. The pump is an electroosmotic pump.Type: GrantFiled: March 26, 2012Date of Patent: December 1, 2015Assignee: General Electric CompanyInventors: Christopher Michael Puleo, Ralf Lenigk
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Patent number: 9176094Abstract: An apparatus (11) for processing a sample, circuit board (8008) suited to use in such an apparatus and a method of processing are provided in which the apparatus (11) is provided with alignment features to assist in provided the circuit board and a sample cartridge (9) in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge (9) are positioned against a plurality of elements, such as a heater (4) for heating the sample cartridge (9) or an electrical contact (EP1-EP4) for powering features on the sample cartridge (9), correctly and can operate independently of one another.Type: GrantFiled: August 25, 2011Date of Patent: November 3, 2015Assignees: WHITESPACE ENTERPRISE CORPORATION, THE ARIZONA BOARD OF REGENTSInventors: Stan Smith, Brett Duane, Cedric Hurth, David Nguyen, Amol Surve, Ralf Lenigk, Frederic Zenhausern
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Publication number: 20150259671Abstract: A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided.Type: ApplicationFiled: July 31, 2012Publication date: September 17, 2015Applicant: GENERAL ELECTRIC COMPANYInventors: Christopher Michael Puleo, Ralf Lenigk, John Richard Nelson, Xiaohui Chen, Li Zhu, Erin Jean Finehout, Patrick McCoy Spooner
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Publication number: 20150233802Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.Type: ApplicationFiled: April 15, 2015Publication date: August 20, 2015Inventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Patent number: 9103331Abstract: An electroosmotic pump comprises a plurality of membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes, a plurality of electrodes comprising cathodes and anodes, and a power source. Each of the positive electroosmotic membranes and negative electroosmotic membranes are disposed alternatively and wherein at least one of the cathodes is disposed on one side of one of the membranes and at least one of the anodes is disposed on other side of the membrane. At least one of the cathodes or anodes is disposed between a positive electroosmotic membrane and negative electroosmotic membrane.Type: GrantFiled: December 15, 2011Date of Patent: August 11, 2015Assignee: General Electric CompanyInventors: Christopher Michael Puleo, Christopher Fred Keimel, Xiaohui Chen, Ralf Lenigk, Craig Patrick Galligan, Todd Frederick Miller
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Patent number: 9063041Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.Type: GrantFiled: November 30, 2012Date of Patent: June 23, 2015Assignee: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Publication number: 20150167052Abstract: A sample storage and extraction device is provided. The sample storage and extraction device includes a substrate frame and a substrate cover. The substrate frame includes a substrate region configured to receive a sample substrate. The sample storage and extraction device further includes a compression assembly configured to provide an isolation zone in a portion of the sample substrate. Moreover, the sample storage and extraction device includes a fluidic channel configured to flow elution fluid to the isolation zone.Type: ApplicationFiled: December 13, 2013Publication date: June 18, 2015Applicant: General Electric CompanyInventors: Weston Blaine Griffin, Erin Jean Finehout, Ying Mao, Ralf Lenigk, Daniel Jason Erno
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Publication number: 20150050654Abstract: The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.Type: ApplicationFiled: August 19, 2013Publication date: February 19, 2015Inventors: Erin Jean Finehout, Christopher Michael Puleo, Ralf Lenigk, Jason M. Nichols, John Nelson
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Publication number: 20150047413Abstract: A method of verifying the performance of a size standard, formed of a plurality of size standard elements, used in a nucleic acid analysis method is provided. The verification is achieved by introducing the size standard to an analysis stage and performing a size based separation on the size standard. The size based separation defines an experimental position for each size standard element. A comparison is made as to whether or not the experimental position for the size standard element corresponds with expected position. The comparison allows a decision to be made on whether or not to use the size standard, which is dependent upon whether or not the experimental position of the size standard element corresponds with the expected position.Type: ApplicationFiled: July 21, 2014Publication date: February 19, 2015Inventors: Frederic ZENHAUSERN, Ralf LENIGK, Andrew HOPWOOD, Pieris KOUMI
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Publication number: 20150027891Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: ApplicationFiled: July 26, 2013Publication date: January 29, 2015Applicant: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Publication number: 20150027894Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.Type: ApplicationFiled: July 26, 2013Publication date: January 29, 2015Applicant: General Electric CompanySchenectadyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Publication number: 20140186841Abstract: A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying,Type: ApplicationFiled: September 23, 2013Publication date: July 3, 2014Inventors: Frederic ZENHAUSERN, Ralf LENIGK, Jianing YANG, Zhi CAI, Alan NORDQUIST, Stanley D. SMITH, David MAGGIANO, Glen MCCARTY, Mrinalini PRASAD, Karem LINAN, Baiju THOMAS, Edward OLAYA, Cedric HURTH, Darryl COX, Mark RICHARD
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Publication number: 20140178938Abstract: A method and device structure are provided which enable an archive sample to be collected and detached relative to a device within which a series of processes, such as PCR are being provided. A chamber structure and method of use are provided in which a controlled and precise volume is obtained by control of the relative resistance to flow through various channels.Type: ApplicationFiled: July 8, 2013Publication date: June 26, 2014Inventors: Frederic ZENHAUSERN, Alan NORDQUIST, Ralf LENIGK, Cedric HURTH, Jianing YANG, Xiaojia CHEN, Matthew ESTES, John LEE-EDGHILL, Nina MORAN, Andrew HOPWOOD, Pieris KOUMI, Zhi CAI
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Publication number: 20140154736Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.Type: ApplicationFiled: November 30, 2012Publication date: June 5, 2014Applicant: GENERAL ELECTRIC COMPANYInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk