Patents by Inventor René Verwaal

René Verwaal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • Patent number: 11149288
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Patent number: 11118193
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: September 14, 2021
    Assignee: DSM IP ASSESTS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 11078504
    Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: August 3, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Zheng Zhao, René Verwaal, Jacobus Thomas Pronk, Rolf Poldermans
  • Publication number: 20210071174
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.
    Type: Application
    Filed: May 6, 2019
    Publication date: March 11, 2021
    Inventors: Johannes Andries ROUBOS, Rene VERWAAL, Brenda VONK
  • Publication number: 20200392513
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a genome editing system.
    Type: Application
    Filed: November 20, 2018
    Publication date: December 17, 2020
    Inventors: Rene VERWAAL, Paulus Petrus DE WAAL, Francine Maruschka Johanna DE LEEUW-VAN LOON
  • Publication number: 20200199599
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: June 25, 2020
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • Publication number: 20200181657
    Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Application
    Filed: December 22, 2016
    Publication date: June 11, 2020
    Inventors: Alrik Pieter LOS, Zheng ZHAO, René VERWAAL, Jacobus Thomas PRONK, Rolf POLDERMANS
  • Publication number: 20200172934
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: June 4, 2020
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20200165637
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: May 28, 2020
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS
  • Publication number: 20200131511
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention trelates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Application
    Filed: July 13, 2017
    Publication date: April 30, 2020
    Inventors: James Casey LIPPMEIER, Yelena BETZ, Johannes Andries ROUBOS, René VERWAAL
  • Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 10590436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: March 17, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Publication number: 20200032252
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
    Type: Application
    Filed: April 4, 2018
    Publication date: January 30, 2020
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • Publication number: 20190185843
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Application
    Filed: December 15, 2016
    Publication date: June 20, 2019
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Blance Elisabeth Maria GIELESEN, Brenda VONK
  • Patent number: 10280439
    Abstract: The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: May 7, 2019
    Assignees: DSM IP ASSESTS B.V., ROQUETTE FRERES SA
    Inventors: Mickel Leonardus August Jansen, René Verwaal, Laurent Segueilha, Mélanie Louchart, Tania Veiga Dos Inocentes
  • Publication number: 20190055544
    Abstract: The present invention relates relates to a CRISPR-CAS system for a host cell, in particular to a method to produce a circular vector comprising one or more guide-polynucleotide expression cassettes, wherein said one or more guide-polynucleotide expression cassettes comprise a polynucleotide encoding a guide-polynucleotide operably linked to one or more control sequences which direct the expression of said guide-polynucleotide in a host cell, wherein said guide-polynucleotide comprises a guide-sequence that essentially is the reverse complement of a target-polynucleotide in a host cell and wherein the guide-polynucleotide can direct binding of a Cas protein at a target-polynucleotide in a host cell to form a CRISPR-Cas complex, in vivo.
    Type: Application
    Filed: July 6, 2016
    Publication date: February 21, 2019
    Inventors: Paulus Petrus DE WAAL, Johannes Andries ROUBOS, Paul KLAASSEN, René VERWAAL
  • Publication number: 20180023096
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 25, 2018
    Applicant: DSM IP ASSETS B.V.
    Inventors: BERNARD MEIJRINK, RENÉ VERWAAL, BIANCA ELISABETH MARIA GIELESEN, JOHANNES ANDRIES ROUBOS
  • Publication number: 20180010151
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 11, 2018
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS