Abstract: A projector system using a screen comprised of either a plurality of miniature, reflective, and concave or convex curved surfaces that are arranged in a pattern to create a screen surface for front projection, or a plurality of miniature, transparent, and curved convex or concave lenses that are arranged in a pattern to create a screen surface for rear projection, and a projector system with the pixel capacity to control the color and brightness focused onto many different subsections of each curved surface. Each curved lens or curved mirror surface is small enough so that when viewed from the viewing area, the lit and unlit areas seen within each individual curved surface blend together so that only the average brightness and color of each curved surface can be seen by a viewer.

Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.

Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.

Abstract: Provided herein are methods for amplification a target dsDNA that is impregnated within a porous matrix using endonuclease-assisted DNA amplification. The amplicons may be subsequent detected within the porous matrix or may be eluted out of the porous matrix. Methods for extracting a genetic material from a biological sample using endonuclease-assisted DNA amplification within a porous matrix are also provided.

Abstract: Disclosed are examples of systems, apparatus, methods and computer program products for maintaining workflows to manage tasks assigned to a user of a computing system. For example, first data indicating a user selection to perform a first action can be received via a computing device associated with the user. A first workflow can be identified from a plurality of unique candidate workflows based at least in part on the first action and on metadata associated with the first action. The plurality of unique candidate workflows can be stored as data objects in a database. Each candidate workflow can comprise a set of tasks assigned to the user. Each set of tasks can be related to performance of one or more aspects of business of an organization with which the user is affiliated. One or more tasks of the first workflow can be identified or generated based at least in part on the first action and on the first workflow.

Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.

Abstract: Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.

Abstract: A well treatment additive includes a siloxane surfactant, a solvent and an aqueous phase. The solvent is preferably a terpene hydrocarbon. Also disclosed is a method for using the well treatment additive to form and enhance the properties of terpene solvent based additives useful for the treatment of oil and gas wells. Methods of using the novel well treatment additives include using the additives in a variety of well treatment processes including, but not limited to, acidizing operations, hydraulic fracturing operations, well remediation operations and water removal operations.

Abstract: An electronic circuit for converting higher-frequency output signals from a sensor into lower-frequency input signals for a discrete input wireless transmitter. The circuit includes a differential amplifier, a digital comparator, a digital frequency divider, and a transistor switch. The differential amplifier amplifies the sensor signals as a function of a frequency of the sensor signals. The digital comparator generates a square wave signal by comparing the amplified sensor signals to a fixed reference voltage. The digital frequency divider generates a lower-frequency signal by dividing the square wave signal. The transistor switch generates the lower-frequency input signals for the discrete input wireless transmitter by switching as a function of the lower-frequency signal from the digital frequency divider.

Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.

Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.

Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.

Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

Abstract: Methods for developing a binding-element are provided. A mixture comprising a target molecule, a plurality of oligonucleotides and a ligase is provided, followed by binding the oligonucleotides to the target molecule to form an oligonucleotides-target molecule complex. The oligonucleotides bound to the target molecule are ligated to form the binding-element. The binding-elements are separated from the mixture.

Abstract: Methods and kits for generating circular nucleic acids in a cell-free system, and uses for the generated circular nucleic acids are provided. The methods comprise in vitro amplification of a nucleic acid template comprising a recombination site to produce tandem repeat nucleic acid sequence, and employ a recombination protein to generate the circular nucleic acids from the tandem repeat nucleic acid sequence.

Abstract: A pump for exchange of fluid between an apparatus reservoir and a reserve reservoir. The pump may have an elongated tubular body with a central bore with a reservoir end portion, an inlet end portion and an intermediate barrel portion. A piston assembly with a driving piston with a stem member with nonmagnetic properties attached and the stem member has a tubular extension member attached with all in longitudinal axis alignment slidably positioned in the central bore to position the driving piston adjacent to an electromagnetic coil assembly that is positioned around the intermediate barrel portion and to position the tubular extension member for a reservoir end to extend outwardly at the reservoir end portion opening. An output tubular member with a pressure relief valve is attached to an output port in a side wall of the reservoir end portion positioned for release of fluid through an output port.

Abstract: The present invention provides a system and method to identify unique browsers (Agents) communicating to the client. Every new browser communicating through the client is assigned a unique Agent ID that is stored in the browser's cookie cache. When the browser sends a request, the cookie may have the Agent ID to identify the browser to the client. If the cookie with the Agent ID is not present, then the client will return a re-direction response to a common URL with the domain having the cookie with the Agent ID to identify the unique browser. Another re-direction takes place back to the browser to make the request with the original URL, but this time has the cookie with the Agent ID to identify the unique browser.

Abstract: A composition includes a first probe, a first initiator component bonded to the first probe, a second probe, and a second initiator component bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte, and the first initiator component and the second initiator component are capable of forming an initiator when present in proximity to each other and when the first probe and the second probe are bonded to the analyte. An associated kit, device, and method are provided.

Abstract: A composition includes a first probe and a first initiator bonded to the first probe. The first probe is capable of binding to an analyte and the first initiator is capable of initiating a controlled polymerization reaction. An associated kit, device, and method are provided.

Abstract: The composition includes a first probe and a first initiator bonded to the first probe. The composition further includes a second probe and a second initiator bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte. An associated kit, device, and method are provided.