Abstract: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

Abstract: Methods of using pharmaceutical tetrahydroisoquinoline compounds for learning and/or memory enhancement, and for treatment of dementia, cocaine dependence, depression, eating disorders, anxiety, or attention deficit disorder with hyperactivity (ADHD).

Abstract: This invention related to constructs comprising mutant HIV genomes having an alteration in a nucleotide sequence which is critical for genomic RNA packaging and non-infectious, immunogenic HIV particles produced by expression of these constructs in mammalian cells. Cell lines which stably produce non-infectious, immunogenic HIV particles are also included. Prophylactic and therapeutic vaccines, diagnostic reagents, and related methods are further described.

Abstract: A video processing system includes but structure, signal flow, and components to process and combine audio and video signals with improved power, cost-effectiveness, and flexibility. The system includes one or more input modules to convert input video signals to a predetermined format. A router routes converted video signals to selected ones of upstream processing cards, which perform video processing such as digital video effects, frame synchronization, non-linear editing, cascading, filtering, and the like. Signals from the upstream processing cards are directed to a switcher, which selectively mixes the upstream-processed signals. A switcher preview module may be provided to intermediately view signals provided by the switcher. The switcher is also attached to a series of downstream video processing cards, which may insert logos, add overlaying, and other suitable post-mixing functions.

Abstract: A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacterial DNA useful as a genetic marker.

Abstract: This invention related to constructs comprising mutant HIV genomes having an alteration in a nucleotide sequence which is critical for genomic RNA packaging and non-infectious, immunogenic HIV particles produced by expression of these constructs in mammalian cells. Cell lines which stably produce non-infectious, immunogenic HIV particles are also included. Prophylactic and therapeutic vaccines, diagnostic reagents, and related methods are further described.

Abstract: A printer blank for a computer system printer is disclosed. The blank has a contiguous pressure adhesive backed label section and may also have a larger plain paper section separated from the label section by a perforation line. The label section is comprised of a plurality of adhesive backed labels and a backing sheet, including a main label portion and at least one smaller auxiliary label. A preferred blank comprises a plurality of auxiliary labels. In use, at least one, but preferably not all, of the auxiliary labels on such a preferred blank are simultaneously removed from the backing sheet with the main label portion. Any auxiliary label(s) simultaneously removed with the main label but not printed upon are readily separated from the main label.

Abstract: Recombinant mycobacterial vaccine vehicles capable of expressing DNA of interest which encodes at least one protein antigen for at least one pathogen against which an immune response is desired and which can be incorporated into the mycobacteria or stably integrated into the mycobacterial genome. The vaccine vehicles are useful for administration to mammalian hosts for purposes of immunization. A recombinant vector which replicates in E. coli but not in mycobacteria is also disclosed. The recombinant vector includes 1) a mycobacterial gene or portions thereof, necessary for recombination with homologous sequences in the genome of mycobacteria transformed with the recombinant plasmid; 2) all or a portion of a gene which encodes a polypeptide or protein whose expression is desired in mycobacteria transformed with the recombinant plasmid; 3) DNA sequences necessary for replication and selection in E. coli; and 4) DNA sequences necessary for selection in mycobacteria (e.g., drug resistance).

Abstract: The present invention relates to recombinant mycobacteria, particularly recombinant M. bovis BCG, which express heterologous DNA encoding a product (protein or polypeptide) of interest, such a protein or polypeptide (e.g., an antigen) against which an immune response is desired, or a cytokine.

Abstract: A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacterial DNA useful as a genetic marker.

Abstract: A roller bracket apparatus includes a roller assembly which includes a carriage portion and a link portion projecting from the carriage portion. A push/pull assembly is provided, and a pivot assembly is connected between the link portion of the roller assembly and the push/pull assembly. The carriage portion includes a wheel-support portion and a plurality of axle/wheel assemblies supported by the wheel-support portion. Each of the axle/wheel assemblies includes an axle and a roller wheel connected to the axle. The link portion extends from the carriage portion at an oblique extension angle. The push/pull assembly includes a first member connected to the pivot assembly, and a second member is connected to the first member. The push/pull assembly also includes a third member connected between the first member and the second member.

Abstract: The present invention relates to recombinant mycobacteria, particularly recombinant M. bovis BCG, which express heterologous DNA encoding a product (protein or polypeptide) of interest, such a protein or polypeptide (e.g., an antigen) against which an immune response is desired, or a cytokine.

Abstract: A eukaryotic, global negative regulator of class II transcription, DNA sequences encoding the negative regulator and results demonstrating the activity of the purified negative regulator protein to repress class II transcription are described.

Abstract: A multiple position work is disclosed caddy seat or stool which has a base and at least two rest surfaces to support a worker. The stool and rest surfaces are constructed and arranged to properly balance the worker's weight and relieve stress and strain on a worker's neck, upper body, and back and to provide comfort to a worker. The work stool also includes a storage compartment for caddying tools and which is easily accessible when used in any of the positions. Additionally, the stool may have limb rests for when the worker is working in a kneeling position. Cushions are provided on the rest surfaces to provide maximum comfort for the worker. The work stool is designed for use by heating, ventilating, and air conditioning technicians, but is readily adaptable to for use by plumbers, electricians, masons, mechanics, janitors, gardeners, and the like.

Abstract: A method for packaging an acoustic wave filter (102). The method includes a step of providing a first wafer (100). The first wafer (100) supports acoustic wave transduction and propagation. The method also includes steps of processing the wafer (100) to provide transducer patterns (18, 18') thereon and disposing a seal ring (25) on the wafer (100). The seal ring (25) completely encloses active areas allowing portions of each bond pad to extend outside of the seal ring. The method further includes steps of disposing a second wafer (40) atop the seal ring (25) and the first wafer (100), sealing the second wafer (40) to the first wafer (100), dicing the second wafer (40) with a saw that provides a first kerf width, whereby portions of the second wafer (40) overlying bonding pads (20) of the transducer patterns (18, 18') are removed and dicing the first wafer (100) with a saw that provides a second kerf width narrower than the first kerf width to provide a packaged SAW die.

Abstract: A printer blank for a computer system printer is disclosed. The blank has a contiguous pressure adhesive backed label section and may also have a larger plain paper section separated from the label section by a perforation line. The label section is comprised of a plurality of adhesive backed labels and a backing sheet, including a main label portion and at least one smaller auxiliary label. A preferred blank comprises a plurality of auxiliary labels. In use, at least one, but preferably not all, of the auxiliary labels on such a preferred blank are simultaneously removed from the backing sheet with the main label portion. Any auxiliary label(s) simultaneously removed with the main label but not printed upon are readily separated from the main label.