Abstract: Vectors that encode Adeno-Associated Virus (AAV) Rep and Cap proteins of different serotypes and Adenovirus transcription products that provide helper functions were used to produce pseudotyped recombinant AAV (rAAV) virions. Purification methods generated pseudotyped rAAV virion stocks that were 99% pure with titers of 1×1012?1×1013 vector genomes/ml.

Abstract: A simultaneously molded plurality of composite shingles or double walled shingles or other substantially planar exterior building products are provided, each being comprised of two or more layers of thermoplastic shingle material, such that a weatherable capstock material is provided outside a core material, in the form of a composite parison that is then blow molded in a hollow mold, to produce novel shingles. Ventilation and/or insulation can be provided to a hollow interior between spaced apart walls of a double walled shingle.

Abstract: A method for performing performance prediction with respect to a vertical flight plan. A system performs performance prediction with respect to a vertical flight plan of an aircraft that includes determining which vertical flight plan rules of a plurality of vertical flight rules that have been loaded in a predictions processor are active by monitoring for criteria that are used to initiate or terminate one or more of the vertical flight plan rules. Aircraft state is predicted at waypoints along a lateral flight path in view of active vertical flight plan rules, and the predicted aircraft state is updated within an integrated flight plan database that stores prediction performance data associated with the aircraft's flight.

Abstract: An impact resistant shingle is provided, wherein the base mat is impregnated with an asphaltic material, with an asphaltic material on the upper surface of the shingle, and wherein another asphaltic material is disposed on the rear surface of the shingle, which other asphaltic material is softer with greater elongation than the asphaltic material used elsewhere in the shingle, such that crack resistance is afforded because energy from impact on the shingle is dissipated.

Abstract: A method of dynamically allocating usage of a shared resource between user A and user B includes the steps of establishing an initial allocation percentage for user B for using the shared resource and establishing a threshold allocation percentage for user B. The allocation percentage is modified for user B based on the availability of the shared resource to user A. If the shared resource is unavailable to user A, the allocation percentage for user B is decreased. If the shared resource is available to user A, the allocation percentage for user B is increased. Usage of the shared resource by user B is allocated in accordance with the modified allocation percentage provided that the modified allocation percentage is less than a pre-set threshold level. The method is particularly useful in dynamically allocating telephone calls into call centers.

Abstract: A process of simultaneously molding either a plurality of composite shingles or double walled shingles or other substantially planar exterior building products is provided, each being comprised of two or more layers of thermoplastic shingle material, such that a weatherable capstock material is provided outside a core material, in the form of a composite parison that is then blow molded in a hollow mold, to produce novel shingles according to the process. Ventilation and/or insulation can be provided to a hollow interior between spaced apart walls of a double walled shingle.

Abstract: A process of simultaneously molding either a plurality of shingles or double walled shingles or other substantially planar exterior building products is provided, such that a blow moldable thermoplastic material is provided, of a composite parison that is then blow molded in a hollow mold, to produce novel shingles according to the process. Ventilation and/or insulation can be provided to a hollow interior between spaced apart walls of a double walled shingle.

Abstract: Systems and methods for improving transducer response sensitivity in an ultrasound-imaging system are disclosed. One method includes selecting a desired transmit spectrum, quantifying the transmit channel impulse response, calculating a drive signal that when applied to the transmit channel will produce the desired transmit spectrum and applying the drive signal to the transducer. The method may be applied in principle to the receive channels. An improved ultrasound-imaging system in accordance with the invention includes a transducer and a switch controlled to apply an excitation signal to the transducer in a transmit mode of the ultrasound-imaging system. A signal shaper is configured to generate an excitation signal in response to the impulse response of the transmit channel such that a desired ultrasound energy waveform is generated by the transducer. An adaptable filter responsive to a desired echo spectrum and the impulse response of the receive channel of the system may be included.

Abstract: Systems and methods for improving transducer response sensitivity in an ultrasound-imaging system are disclosed. One method includes selecting a desired transmit spectrum, quantifying the transmit channel impulse response, calculating a drive signal that when applied to the transmit channel will produce the desired transmit spectrum and applying the drive signal to the transducer. The method may be applied in principle to the receive channels. An improved ultrasound-imaging system in accordance with the invention includes a transducer and a switch controlled to apply an excitation signal to the transducer in a transmit mode of the ultrasound-imaging system. A signal shaper is configured to generate an excitation signal in response to the impulse response of the transmit channel such that a desired ultrasound energy waveform is generated by the transducer. An adaptable filter responsive to a desired echo spectrum and the impulse response of the receive channel of the system may be included.

Abstract: The present invention is directed to a pad for carrying liquid having a piece of non-woven material wherein the piece of material is capable of absorbing an amount of the liquid at least about 1.5 times the weight of the piece of material, a first printed ink on a first surface of the material, and a second printed ink on either the first or a second surface of the material, and wherein the first ink is substantially in register with the second ink. The present invention is also directed to a method for making a printed pad for carrying a liquid including the steps of feeding a web of non-woven material into a rotogravure press, wherein the material is capable of absorbing at least about 1.5 times its own weight of the liquid, printing a first ink with the press on a first surface of the web, printing a second ink with the press substantially in register with the first ink on the first surface or a second surface of the web.

Abstract: The present invention is directed to a pad for carrying liquid having a piece of non-woven material wherein the piece of material is capable of absorbing an amount of the liquid at least about 1.5 times the weight of the piece of material, a first printed ink on a first surface of the material, and a second printed ink on either the first or a second surface of the material, and wherein the first ink is substantially in register with the second ink. The present invention is also directed to a method for making a printed pad for carrying a liquid including the steps of feeding a web of non-woven material into a rotogravure press, wherein the material is capable of absorbing at least about 1.5 times its own weight of the liquid, printing a first ink with the press on a first surface of the web, printing a second ink with the press substantially in register-with the first ink on the first surface or a second surface of the web.

Abstract: A flexible manufacturing line and manufacturing process employs an overhead gantry system to transfer fixtured workpieces between machining stations served by the gantry system. The machining stations include CNC machines, turret cells, and/or dedicated machines which receive the fixtured workpieces through top-entry openings. The invention provides flexibility and adaptability to variation, improved maintenance of reference position on the fixture during manufacture, improved accessibility to machining stations during operation, and increased safety.

Abstract: Systems and methods for improving transducer response sensitivity in an ultrasound-imaging system are disclosed. A preferred method generally includes the steps of: selecting a desired transmit spectrum; quantifying the transmit channel impulse response; calculating a drive signal that when applied to the transmit channel will produce the desired transmit spectrum; and applying the drive signal to the transducer. Other methods include selecting a desired echo response spectrum; quantifying a receive channel impulse response; formulating a filter that when applied to received ultrasound echo signals will produce the desired echo response spectrum; and applying the filter.

Abstract: The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP gene at the transcriptional and post-translational level.

Abstract: The present invention is directed to a pad for carrying liquid having a piece of non-woven material wherein the piece of material is capable of absorbing an amount of the liquid at least about 1.5 times the weight of the piece of material, a first printed ink on a first surface of the material, and a second printed ink on either the first or a second surface of the material, and wherein the first ink is substantially in register with the second ink. The present invention is also directed to a method for making a printed pad for carrying a liquid including the steps of feeding a web of non-woven material into a rotogravure press, wherein the material is capable of absorbing at least about 1.5 times its own weight of the liquid, printing a first ink with the press on a first surface of the web, printing a second ink with the press substantially in register with the first ink on the first surface or a second surface of the web.

Abstract: The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP gene at the transcriptional and post-translational level.

Abstract: The instant invention provides methods and materials for expressing a polypeptide with factor VIII activity comprising administering an rAAV vector encoding a truncated version of human factor VIII, containing, for example, a 90 kD heavy chain of factor VIII fused to a light chain of factor VIII.

Abstract: Apparatus and methods for manufacturing electrochromic cells. Layers of various inks are printed on substrates by high speed printing means. The electrochromic cells comprise layers of electrode, electrolyte, and counter electrode ink materials which are printed on at least one substrate. When an electrical voltage differential is introduced between the electrode and counter electrode layers, an electrochemical reaction occurs in the electrochemical cell.

Abstract: An engine filter assembly for cleaning cooling air for an internal-combustion engine for use in high-debris environment such as in roofing-removing machines includes a filter housing attached to the machine at a remote position. The filter housing houses a two-stage air filter that consists of a cylindrical-shaped filter element with an outer pre-filter covering. Both the carburetor air inlet and a fan housing leading to the cooling fan on the engine are connected to the filter housing by tubing. A shield protects the engine from direct contact with loose or air-borne materials during the operation of the machine. A thermal sensing device reads the temperature of the engine and automatically shuts down the engine if the temperature rises beyond specification, reducing the chance of engine failure.

Abstract: An ultrasound imaging system includes an array of transducer elements, a predetermined number of transducer and receiver channels less than the number of transducer elements in the array, and an electronic switch for selecting transducer elements for connection to the transmitter and receiver channels. A method using the imaging system for ultrasound imaging with a sector scan pattern includes determining, from a sector aperture size and a sector aperture location on the array, a mapping of the transducer elements to the transmitter and receiver channels. Focusing delays are determined from a sector angle and a focus for the sector scan pattern, and the focusing delays are converted to mapped focusing delays using the mapping. Ultrasound energy is transmitted and received along sector scan lines of the sector scan pattern in response to the mapped focusing delays, and signals representative of an image along the sector scan lines are provided.