Abstract: The present invention provides methods for diagnosing and treating cancer, and in particular methods for determining the susceptibility of subjects suspected of having breast cancer (or known to have breast cancer) to treatment with topoisomerase II inhibitors. The present invention also provides in situ hybridization probes for specifically detecting topoII&agr; gene sequences.

Abstract: A method of enhancing heat transfer and cooling efficiency in a cooling passage includes forming a plurality of turbulator rings in the passage, the rings projecting inwardly, substantially perpendicular to a cooling flow direction in the passage; and using a patterned electrode, forming at least one gap in one or more of the turbulator rings, extending substantially parallel to the flow direction.

Abstract: The present invention relates to chromogenic (colorimetric) in situ hybridization (CISH) and nucleic acid probes useful for in situ hybridization. Specifically, the present invention provides methods, kits, and compositions for performing bright field cancer diagnostics employing chromogenic in situ hybridization (e.g. to detect gene amplifications, gene translocations, and chromosome polysomy). In preferred embodiments, the present invention provides CISH methods, kits and compositions for detecting HER2 gene status.

Abstract: A steam turbine wheel has a male dovetail projecting about its peripheral margin and a gap in the margin. Buckets having bases with complementary female dovetails are disposed in the gap and register with the male dovetail of the wheel, enabling the buckets to slide about the wheel into positions stacking the bases of the buckets against one another. The final three buckets including a closure bucket are disposed about the wheel margin with two buckets straddling the closure bucket secured to the wheel margin by the dovetails. The closure bucket is secured to the straddling buckets by axially extending pins. The closure and straddling buckets, as well as the pins, are formed of materials having a higher creep rupture strength than the materials forming the remaining buckets, enhancing the capability of the closure bucket to withstand high temperature and stresses over time without creep.

Abstract: A multiple hook dovetail connection for connecting a rotor wheel and a bucket for a turbine rotor that permits the use of wider vanes at the ends of the buckets without changing the size of the wheel and the other existing components of the turbine. The dovetail connection comprises a male dovetail component and a female dovetail component. The male dovetail component includes a plurality of hooks. Each of the hooks includes a crush surface, a neck and an angle formed between the crush surface and the neck. These books are dimensioned in accordance with at least one of the included tables.

Abstract: A multiple hook dovetail connection for connecting a rotor wheel and a bucket of a turbine rotor that permits the use of wider vanes at the ends of the buckets without changing the size of the wheel and the other existing components of the turbine. The dovetail connection comprises a male dovetail component and a female dovetail component. The male dovetail component includes first and second hooks. Each of the hooks includes a crush surface, a neck and an angle formed between the crush surface and the neck. These hooks are dimensioned in accordance with at least one of the included tables.

Abstract: An annular turbine shroud separates a hot gas path from a cooling plenum containing a cooling medium. Bumps are cast in the surface on the cooling side of the shroud. A surface coating overlies the cooling side surface of the shroud, including the bumps, and contains cooling enhancement material. The surface area ratio of the cooling side of the shroud with the bumps and coating is in excess of a surface area ratio of the cooling side surface with bumps without the coating to afford increased heat transfer across the element relative to the heat transfer across the element without the coating.

Abstract: A method of enhancing heat transfer and cooling efficiency in a cooling passage includes forming a plurality of turbulator rings in the passage, the rings projecting inwardly, substantially perpendicular to a cooling flow direction in the passage; and using a patterned electrode, forming at least one gap in one or more of the turbulator rings, extending substantially parallel to the flow direction.

Abstract: The embodiments of the invention are multiple antibody immunoassay systems, and preferably immunohistochemical staining systems. In these systems the detection antibody system that detects the association of a targeting antibody (that binds to the target moiety to be detected) may also cross-react with endogenous immunoglobulins in the specimen in which the target moiety is to be detected, yielding an unreliable assay with high background levels. This problem has been overcome by a process that includes cross-linking a monovalent antibody to the endogenous antigenic determinants prior to exposure to the targeting antibody and detecting antibody system. The embodiments of the invention also include kits for use in these multiple antibody immunoassay and histochemical staining systems.

Abstract: A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.

Abstract: A method of immunologically staining a formalin-fixed tissue preparation, which comprises (a) subjecting a formalin-fixed tissue preparation to microwave energy while the tissue preparation is submersed in water for a time sufficient to increase immunostaining efficiency; (b) removing the tissue preparation from the water and cooling; and (c) contacting the tissue preparation with an immunological staining reagent.