Abstract: Mass spectrometry techniques for determining the status of sepsis in an individual are provided. A biomarker profile resolved from a biological sample, taken from the individual, using a mass spectrometry technique is compared to a reference biomarker profile. A single such comparison classifies the individual as belonging to or not belonging to a reference population. The individual's biomarker profile and the reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons. The plurality of ions can be detected by electrospray ionization mass spectrometry in positive mode. The comparison uses a decision rule, such as a classification tree, that determines the status of sepsis in the individual without requiring knowledge of the identity of the biomarkers in the biomarker profile from the individual and without requiring knowledge of the identity of the biomarkers in the reference biomarker profile.

Abstract: The present invention provides methods and compositions useful for the diagnosis or prognosis of a systemic inflammatory condition such as sepsis.

Abstract: The present invention pertains to methods of quantifying the levels of at least one analyte in a sample or extract comprising adding a known quantity of at least one internal standard to the sample or extract. The present invention also relates to internal standards used in mass spectrometry, as well as compositions thereof. Internal standards for mass spectrometry according to the invention can be used, for example, to assist aligning mass spectra obtained from two different samples, each of which comprises the internal standard. In one aspect of the invention, the internal standard is a dendrimer. A labile internal standard may be used in conjunction with the dendrimer.

Abstract: This invention relates to the improvement of arrays of porous polymer pads on solid supports used in biological assays. The invention involves freeze drying the porous polymer pads to increase pore size. The increased pore size results in an enhanced ability of the porous polymer pads to bind specific binding substances such as DNA, RNA and polypeptides.

Abstract: The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished by comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population who develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated at the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms.

Abstract: The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished using a molecular diagnostics approach, involving comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population who develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated at the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms.

Abstract: The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished by comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population that develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated from the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms of sepsis.

Abstract: The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished using a molecular diagnostics approach, involving comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population that develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated at the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms of sepsis.

Abstract: This invention relates to the detection of molecular interactions between biological molecules. Specifically, the invention relates to electrical detection of interactions such as hybridization between nucleic acids or peptide antigen-antibody interaction using arrays of peptides or oligonucleotides. In particular, the invention relates to an apparatus and methods for detecting nucleic acid hybridization or peptide binding using electronic methods including AC impedance. In some embodiments, no electrochemical or other label moieties are used. In others, electrochemically active labels are used to detect reactions on hydrogel arrays, including genotyping reactions such as the single base extension reaction.

Abstract: The present invention provides an apparatus and methods for efficient, high-throughput electrical or electrochemical detection of biomolecules. More specifically, the invention provides an apparatus in which an independent set of electrodes is used to increase the occurrence of a desired bio-conjugation event at a test site.

Abstract: This invention relates to apparatus and methods for detecting single base extension to an oligonucleotide array using electrochemical labels.

Abstract: This invention relates to apparatus and methods for detecting single base extension to an oligonucleotide array using electrochemical labels.

Abstract: This invention relates to the detection of biomolecules. Specifically, the invention relates to electronic or electrochemical detection of biomolecules using biochip arrays. In particular, the invention provides an apparatus comprising a platform for a column-and-row addressable, high-density, enhanced-sensitivity biochip array, and methods of use thereof. The devices and methods of the invention can be used to detect molecular interactions such as nucleic acid hybridization or protein binding.

Abstract: This invention relates to apparatus and methods for detecting single base extension to an oligonucleotide array using electrochemical labels.

Abstract: The present invention provides an apparatus and methods for detecting cation interactions associated with molecular interactions using AC impedance, but without the use of electrochemical or other reporters to obtain measurable signals. The methods can be used for electrical detection of molecular interactions between probe molecules bound to defined regions of an array and target molecules which are permitted to interact with the probe molecules.

Abstract: The present invention provides a method to characterize, optimize, and control the quality and production of hydrogel media. Additionally, the present invention provides a method for determining the size, size distribution, and spatial distribution of the pores of a porous substrate. More particularly, the invention provides a method for determining the size, size distribution, and spatial distribution of the pores of a polymeric hydrogel-based substrate.

Abstract: An organic electroluminescent display device (10) includes a plurality of organic layers (16, 18, 20, 22 and 24) disposed between opposing electrodes (14 and 26). The hole transporting layer (22) comprises a hole transporting material characterized by the general formula: ##STR1## where n, m, and p each independently take the value of 0, 1 or 2; A1 and A2 each represents:(a) an alkyl group; or(b) an alkyl substituted with aryl, hydroxy, cyano, alkoxy, halo, or amino group(s); or(c) an aryl group; or(d) an aryl group substituted with alkyl, aryl, hydroxy, cyano, alkoxy, phenyl, halo, or amino group(s).

Abstract: An organic electroluminescent display device (10) includes a plurality of organic layers (16, 18, 20, 22 and 24) disposed between opposing electrodes (14 and 26). The hole transporting layer (22) is composed of a hole transporting material stabilized by a polymeric network.

Abstract: An organic electroluminescent display device (10) includes a first display region (12) and a second display region (14), each region adapted to provide a different type of information. For example, first display region (12) may be adapted to display icon information, while second display region (14) may be adapted to provide dynamic or changing information such as video or alpha numeric information. The display device (10) may include an organic electroluminescent display module (62) having a plurality of picture elements, said picture elements optionally having corresponding light influencing elements (58 and 102-110) for providing full color icon or dynamic information.

Abstract: An organic electroluminescent display device (10) includes a plurality of organic layers (16, 18, and 20) disposed between opposing electrode assemblies (12 and 14). One electrode assembly (12), the anode assembly, is fabricated of a substrate (22) and electrode (24), and a layer of a compensating material (26) disposed between electrode (24) and the overlying layers of light emitting materials (16, 18, 20). Layer (26) compensates for physical mismatches between the inorganic electrode layer (24) and the organic light emitting layers (16, 18, 20).