Abstract: Methods and systems for single molecule sequencing using concatemers of copies of sense and antisense strands. Concatemers are provided, for example, by carrying out rolling circle amplification on a circular molecule having sense and antisense regions to produce repeated copies of the sense and antisense regions connected by linking regions. The circular molecules can be produced by ligating hairpin adapters to each end of a double-stranded nucleic acid having a sense and antisense strand. The ligations can be carried out, for example using blunt end ligation. In some cases, a single molecule consensus sequence for a single template molecule is obtained. A single read from each template molecule can be obtained by comparing the sequence information of the sense and antisense regions.

Abstract: Disclosed is a virtual wireless base station that can dynamically scale its capacity to meet changes in demand for connectivity. The virtual wireless base station includes a plurality of virtual baseband modules, a plurality of interface/router modules, an orchestrator module and a fabric mapper module. Each of the plurality of virtual baseband modules is coupled to the interface/router modules by a low latency switch fabric. The orchestrator determines current and near future demand for connectivity within the virtual wireless base station and either instantiates and connects new virtual baseband processors to meet a rise in demand, or shuts down underutilized virtual baseband processors in case of insufficient demand.

Abstract: Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract: Optical analytical devices and their methods of use are provided. The devices are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices include integrated illumination elements and optical waveguides for illumination of the optical reactions. The devices further provide for the efficient coupling of optical excitation energy from the waveguides to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices of the invention are well suited for miniaturization and high throughput.

Abstract: Optical analytical devices and their methods of use are provided. The devices are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices include optical waveguides for illumination of the optical reactions. The devices further provide for the efficient coupling of optical excitation energy from the waveguides to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices of the invention are well suited for miniaturization and high throughput.

Abstract: Disclosed is a method for processing code blocks as implemented by a baseband processor. The method involves performing a cyclic redundancy check on decoded and deinterleaved code blocks until one fails its CRC check. On first failure the baseband processor requests a retransmission of the code blocks and resumes CRC checks on the retransmitted code blocks, beginning at the code block that had failed. In the event of subsequent failures, the baseband processor performs a soft combine on the failed retransmitted block with its original transmitted counterpart. Only if the soft combined code block fails does the baseband processor request another retransmission. In this case, subsequent CRC failures result in soft combines of three corresponding code words, making the process more robust. The method reduces the number of retransmissions as well as the computing resources needed for processing incoming code blocks.

Abstract: A control system for a perfusion system (1), the control system being configured to control a plurality of blood flow rates in the perfusion system during a weaning phase. The perfusion system comprises: a first blood line (26) in which blood is permitted to flow at a first flow rate; a second blood line (34) in which blood is permitted to flow at a second flow rate; an arterial blood line (22) in which blood is permitted to flow at an arterial flow rate; and an arterial pump (20) configured to circulate blood at the arterial flow rate in the arterial blood line. The control system comprises a controller configured to determine the first flow rate and the second flow rate and to process the first and second flow rates to determine a desired arterial flow rate. The controller is configured to operate in a first mode in which the controller modulates operation of the arterial pump (20) to adjust the arterial flow rate so that the arterial flow rate matches the desired arterial flow rate.

Abstract: Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of the initial population enriched for a target region, which is typically located within one or more target fragments. The methods are particularly useful for analyzing populations having a high degree of complexity, e.g., chromosomal-derived DNA, whole genomic DNA, or mRNA populations.

Abstract: Disclosed is a mobile edge computing system that provides localized emergency responses and warning exclusively to UEs within a venue or area, and provides location-specific warning within the venue or area. The system includes a component that aggregates the S1-mme interface between the MME and a plurality of baseband units and provides read and write access to the S1-mme interface. The disclosed mobile edge computing system can issue cell-specific public warning system (PWS) messages that are customized for and transmitted exclusively to each individual cell within the venue.

Abstract: An exhaust gas flow control system for an oxygenator of an extracorporeal ventilation system connected to an oxygenation gas supply line and to an exhaust line for removal of exhaust gas comprises a flow control path, a pressure control path, an exhaust flow regulator responsive to the controller, and an exhaust gas pressure regulator responsive to a controller configured to maintain a pre-determined pressure level in the exhaust line. This provides a better degree of control over the pressure across the oxygenator from oxygenation gas inlet to exhaust.

Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.

Abstract: Disclosed herein are classifier models, computer implemented systems, machine learning systems and methods thereof for classifying clinical variants of unknown or uncertain significance into a pathogenicity category using measured phenotype features extracted from phenotype assays of transgenic organism expressing the human clinical variant. Embodiments of the present invention relate generally to methods for generating classifier models using machine learning and use of those classifier models to predict the pathogenicity of a clinical variant for a specific human disease (e.g. genetic disease), assigning a patient clinical variant to a pathogenicity category (e.g. pathogenic or benign) for the specific human disease to determine whether that patient should be followed up with additional, more invasive diagnostic testing, or treatment.

Abstract: Disclosed are various examples for Internet of Things (IoT) device discovery and deployment. In some embodiments, a device identifier is received from an IoT device. The IoT device is determined, based on the device identifier, to be associated with a device account with a management service. An enrollment of the IoT device is performed. A capabilities declaration is received from the IoT device. IoT device instructions are determined based on the capabilities declaration. IoT device instructions are transmitted to the IoT device, causing it to perform a capability specified in the capabilities declaration.

Abstract: Various examples relate to detecting vulnerabilities in managed client devices. In some examples, a system determines whether a vulnerability scan of a computing device is required to be performed. The system installs a vulnerability detection component in the computing device in response to determining that the vulnerability scan is required to be performed. The system requests the vulnerability detection component to perform the vulnerability scan of the computing device. The system transmits a result of the vulnerability scan to a remote management service for the computing device.

Abstract: A clamping mechanism (30) for clamping a flexible tube comprises two rotatable bobbins (22, 32), each with a tube-engaging surface portion (24) defining boundaries of: a free space through which a tube may extend. The shape of: the tube-engaging surface portion (24) changes around the bobbin circumference, such that axial rotation of the bobbins (22, 32) reduces the free space and thereby squeezes the tube. This allows the flow through the tube to be altered dependent on the rotation of: the bobbins.

Abstract: Disclosed is a virtual base station capable of hosting multiple network operators and/or private networks in a single compute environment. The virtual base station includes a plurality of virtual baseband processors configured to communicate with the plurality of mobile network operators, a supervisor module, a fronthaul network interface configured to be coupled to one or more remote units, and a KPI (Key Performance Indicator) coordinator module coupled to the supervisor module and the one or more virtual baseband processors. The base station may have on or more CBRS (Citizens Broadband Radio Service) Daemons to act as a proxy for obtaining grants to CBRS channels and allocating the CBRS channels to the mobile network operators.

Abstract: Disclosed are various examples for distributed profile and key management. In one example, a client device can include an agent application and a PIV-D application. The agent application can receive a partially populated device profile generated by a management service to configure a setting on the client device. The PIV-D application can generate a derived credential and provide the derived credential to the agent application. The agent application can modify the partially populated device profile to include the credential to create a fully populated device profile and configure the client device in accordance with the fully populated device profile.

Abstract: A method of controlling thermal transfer in a perfusion system heat exchanger of an extracorporeal fluid treatment device for conditioning an extracorporeal patient fluid for administration to a patient comprises a step of providing a perfusion system heat exchanger, wherein the perfusion system heat exchanger comprises a first fluid passage for a liquid heat transfer medium and a second fluid passage for the extracorporeal patient fluid to be temperature-controlled via exchange of thermal energy with the heat transfer medium, and a step of providing the heat transfer medium through the first fluid passage. The heat transfer medium comprises a component with anti-microbial properties, such as glycol. The provision of antimicrobial fluid reduces the risk of microbe contamination of the extracorporeal fluid, and hence the risk of clinical complications.

Abstract: The disclosure relates to detecting vulnerabilities in managed client devices. A system determines whether a vulnerability scan of a computing device is required to be performed. The system installs a vulnerability detection component in the computing device in response to determining that the vulnerability scan is required to be performed. The system requests the vulnerability detection component to perform the vulnerability scan of the computing device. The system transmits a result of the vulnerability scan to a remote management service for the computing device.