Abstract: Disclosed herein are methods and compositions for detecting the presence in a sample of a US-type or a US-subtype hepatitis E virus, including naturally occurring variants thereof. In particular, the invention provides nucleic acid sequences corresponding to the genome of the US-type or US-subtype hepatitis E virus, amino acid sequences, including epitope sequences, encoded by the genomes of such viruses, and antibodies that bind specifically to such amino acid sequences. The invention further provides methods and compositions for immunizing individuals against infection by, or for treating individuals already infected with such a virus.

Abstract: Method and apparatus for doubling the throughput rate of data transmission on a logic path comprising providing two latches that alternately receive successive bits of the data stream to be transmitted and a multiplexer having data transmission paths that are alternately clocked by two separate clocks, which clocks are substantially 180 degrees out of phase.

Abstract: A configuration memory architecture for an FPGA eliminates the need for a regular array of word lines and bit lines. The memory includes memory bytes, each of which has eight SRAM latches, a single flip-flop and a one-of-eight decoder having data input coupled to the inverting output of the flip-flop and eight individual data outputs, each of which is coupled to a data input of one of the SRAM latches. The flip-flops of all memory bytes for a logic block are coupled together in a serpentine shift register. Loading of configuration data involves shutting down all paths through the decoder, shifting all configuration bits for the “0” position SRAM latch of each memory byte into the shift register, and setting the address bits to the decoder so as to create a conductive path on each memory byte from the output of the flip-flop to the data input of the 0 latch. The process is then repeated for the seven other SRAM latch positions.

Abstract: A system for controlling the impedances of circuits on an integrated circuit chip is provided. At least one circuit is selected to operate as a p-channel reference circuit, and at least one circuit is selected to operate as an n-channel reference circuit. Other circuits are selected to operate as circuits and/or line termination circuits. A digitally controlled impedance (DCI) circuit controls the p-channel reference circuit to determine a desired configuration of p-channel transistors for use in the circuits. The DCI circuit further controls the n-channel reference circuit to determine a desired configuration of n-channel transistors for use in the circuits. The DCI circuit takes into account such factors as resistances of p-channel transistors in the p-channel reference circuit, resistances of n-channel transistors in the n-channel reference circuit, as well as temperature, voltage and process variations.

Abstract: Method and apparatus for doubling the throughput rate of data transmission on a logic path comprising providing two latches that alternately receive successive bits of the data stream to be transmitted and a multiplexer having data transmission paths that are alternately clocked by two separate clocks, which clocks are substantially 180 degrees out of phase.

Abstract: The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method.

Abstract: Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Abstract: A system for controlling the impedances of circuits on an integrated circuit chip is provided. At least one circuit is selected to operate as a p-channel reference circuit, and at least one circuit is selected to operate as an n-channel reference circuit. Other circuits are selected to operate as circuits and/or line termination circuits. A digitally controlled impedance (DCI) circuit controls the p-channel reference circuit to determine a desired configuration of p-channel transistors for use in the circuits. The DCI circuit further controls the n-channel reference circuit to determine a desired configuration of n-channel transistors for use in the circuits. The DCI circuit takes into account such factors as resistances of p-channel transistors in the p-channel reference circuit, resistances of n-channel transistors in the n-channel reference circuit, as well as temperature, voltage and process variations.

Abstract: Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Abstract: A system for controlling the impedances of circuits on an integrated circuit chip is provided. At least one circuit is selected to operate as a p-channel reference circuit, and at least one circuit is selected to operate as an n-channel reference circuit. Other circuits are selected to operate as circuits and/or line termination circuits. A digitally controlled impedance (DCI) circuit controls the p-channel reference circuit to determine a desired configuration of p-channel transistors for use in the circuits. The DCI circuit further controls the n-channel reference circuit to determine a desired configuration of n-channel transistors for use in the circuits. The DCI circuit takes into account such factors as resistances of p-channel transistors in the p-channel reference circuit, resistances of n-channel transistors in the n-channel reference circuit, as well as temperature, voltage and process variations.

Abstract: Described are systems for producing differential logic signals. These systems can be adapted for use with different loads by programming one or more programmable elements. One embodiment includes a series of driver stages, the outputs of which are connected to one another. The driver stages turn on successively to provide increasingly powerful differential amplification. This progressive increase in amplification produces a corresponding progressive decrease in output resistance, which reduces the noise associated with signal reflection. The systems can be incorporated into programmable IOBs to enable PLDs to provide differential output signals.

Abstract: The present invention relates to nucleic oligomer primers or probes useful for detection of TTV in test samples. Also provided are assays which utilize these primers and probes, as well as test kits which contain these oligomer primers and/or. In addition, the present invention encompasses the use of TTV nucleotide sequences as nucleic acid vectors and as markers for determining transmission between individuals as well as the route thereof. Additionally, the present invention invention encompasses a method of detecting TTV infection prior to xenotransplantation of a tissue or organ.

Abstract: A configuration memory architecture for an FPGA that eliminates the need for a regular array of word lines and bit lines is disclosed. The memory is comprised, in the preferred embodiment, of a plurality of memory bytes. Each memory byte has eight SRAM latches, a single flip flop and a one-of-eight decoder having a data input coupled to the inverting output of the flip flop and eight individual data outputs, each of which is coupled to a data input of one of the SRAM latches. The decoder also has address and write control inputs which are coupled to a state machine or other programmable device that controls the sequencing of the loading operation to load configuration data into the memory. The flip flops of all the memory bytes are coupled together in a serpentine shift register.

Abstract: The present invention provides unique recombinant antigens representing distinct antigenic regions of the Hepatitis C Virus (HCV) genome which can be used as reagents for the detection of antibodies and antigen in body fluids from individuals exposed to HCV. The present invention also provides an assay for detecting the presence of an antibody to an HCV antigen in a sample by contacting the sample with the recombinant antigens. Preferred assay formats include a screening assay, a confirmatory assay, a competition or neutralization assay and an immunodot assay.

Abstract: Recombinantly produced hepatitis GB Virus (HGBV) amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV amino acid sequences and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV recombinantly produced amino acid sequences.

Abstract: The present invention provides a method of synthesizing genes encoding unique HIV-1 and HIV-2 envelope proteins and their fragments, thereby allowing overexpression of these proteins in E. coli. The HIV envelope proteins and their fragments have been expressed at high levels as individual proteins or in fusion with other proteins. The HIV envelope proteins thus expressed in E. coli can be effectively used for the detection of exposure to HIV as well as the discrimination of HIV-1 and HIV-2.

Abstract: Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Abstract: Hepatitus GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and theraeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Abstract: Reagents and composition for controlling the translation of hepatitis GB virus (HGBV)-A, -B or -C peptides from viral nucleic acid. These reagents and methods comprise control elements of the 5' NTR region of the HGBV-A, -B, or -C viral genome.

Abstract: A duty cycle controller for generating proper control signals for an SRAM in an FPGA in the proper sequence and spaced at the proper times to guarantee proper operation of the SRAM regardless of the frequency of duty cycle of the clock selected by the user to synchronize and drive operations of the SRAM.