Abstract: The present invention provides a method for detecting a monoclonal antibody in a sample, the method comprising: (a) a step of capturing and immobilizing, in pores of a porous body, the monoclonal antibody in the sample; (b) a step of performing selective protease digestion of the monoclonal antibody for 30 min or longer by contacting the porous body having the monoclonal antibody immobilized thereon with nanoparticles having a protease immobilized thereon; and (c) a step of detecting a peptide fragment obtained by the selective protease digestion, using liquid chromatography mass spectrometry (LC-MS), wherein step (b) is carried out under stirring condition for 10 sec to 5 min in the initial reaction stage, and then under static condition. According to the present invention, the detection method of a monoclonal antibody using mass spectrometry is simplified and can be applicable to multisample analysis.

Abstract: The present invention provides a method in which a porous body having a monoclonal antibody to be measured immobilized in pores thereof is brought into contact with nanoparticles having a protease immobilized thereonto in a liquid to perform selective protease digestion of the monoclonal antibody and a peptide fragment obtained by the digestion is detected by liquid chromatography mass spectrometry (LC-MS), wherein the monoclonal antibody is digested with the protease in the presence of an antibody specifically binding to the monoclonal antibody or a target molecule of the monoclonal antibody.

Abstract: Along dicing lines, cutting grooves that reach a rear surface from a front surface are formed by a first dicing blade in a semiconductor wafer, completely separating the semiconductor wafer into individual semiconductor chips by the cutting grooves. Thereafter, by a second dicing blade that is constituted by abrasive grains having a mean grit size smaller than that of the first dicing blade and that has a blade width wider than that of the first dicing blade, side walls of the cutting grooves, i.e., side surfaces of the semiconductor chips are polished, approaching a specular state.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A molding includes a first molding portion having an upper surface bonded to an inner side surface of a windshield, a second molding portion, and a third molding portion extending from the second molding portion so as to extend along an imaginary extension of an outer side surface of the windshield. The third molding portion is formed in a rectangular shape in longitudinal section and has an upper surface disposed at a lower position than the imaginary extension of the outer side surface of the windshield.

Abstract: A vehicle braking device includes a master cylinder device, a motor cylinder device, first and second shutoff valves (solenoid valves) of a normally-open type which are provided in piping tubes (hydraulic pressure passages) providing communication between the master cylinder device and the motor cylinder device and which operate to open or close the hydraulic pressure passages, and an integrated control unit which performs drive control to close the solenoid valves in response to a pressure rise request. When a pressure rise request is received from any requester, the integrated control unit sets a valve closing characteristic in the course of closing the solenoid valves from the time of reception of the pressure rise request such that the valve closing characteristic for the pressure rise request issued by a second group requester is gentler than the valve closing characteristic for the pressure rise request issued by a first group requester.

Abstract: A brake lamp control device is provided which includes: a brake state signal output unit configured to output a brake state signal indicative of ON and OFF states of automatic braking operation for a brake of a vehicle; and a turn-on controller configured to control turn-on and turn-off states of a brake lamp on the basis of the brake state signal and turns on the brake lamp for a predetermined interval after the brake state signal becomes the OFF state.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: The present invention provides a technique that can simultaneously analyze a plurality of antibody drugs and can be validated. The present invention provides a method of bringing a porous body in which monoclonal antibodies to be measured are immobilized in pores into contact in a liquid with nanoparticles on which proteases are immobilized and performing selective proteolysis of monoclonal antibodies to detect peptide fragments each comprising unique amino acid sequence derived from the Fab region of the monoclonal antibodies via liquid chromatography-mass spectrometry (LC-MS), wherein peptide fragments of two or more types of monoclonal antibodies in the same biological sample are simultaneously quantified.

Abstract: Along dicing lines, cutting grooves that reach a rear surface from a front surface are formed by a first dicing blade in a semiconductor wafer, completely separating the semiconductor wafer into individual semiconductor chips by the cutting grooves. Thereafter, by a second dicing blade that is constituted by abrasive grains having a mean grit size smaller than that of the first dicing blade and that has a blade width wider than that of the first dicing blade, side walls of the cutting grooves, i.e., side surfaces of the semiconductor chips are polished, approaching a specular state.

Abstract: A method is provided for more easily detecting and quantifying a protein by regioselectively digesting a variable region of an Fab domain of a monoclonal antibody while suppressing proteolysis of an Fc domain. In the method, a porous body in which a monoclonal antibody is immobilized in pores and nanoparticles on which a protease is immobilized are brought into contact with each other in a liquid to perform selective proteolysis of the monoclonal antibody, and a resulting peptide fragment is detected using liquid chromatography-mass spectrometry (LC-MS), and a peptide having an amino acid sequence that includes an amino acid derived from a CDR2 region of a heavy chain or a light chain of the monoclonal antibody is detected.

Abstract: To provide an antibody against FGF23 and a pharmaceutical composition such as a preventive or therapeutic agent which can prevent or treat by suppressing an action of FGF23 by using the antibody. An antibody or its functional fragment against human FGF23 produced by hybridoma C10 (Accession No. FERM BP-10772).

Abstract: To provide an antibody against FGF23 and a pharmaceutical composition such as a preventive or therapeutic agent which can prevent or treat by suppressing an action of FGF23 by using the antibody. An antibody or its functional fragment against human FGF23 produced by hybridoma C10 (Accession No. FERM BP-10772).

Abstract: A stampable sheet includes a resin and carbon fiber sheet including fiber bundles of discontinuous carbon fibers, wherein the carbon fiber sheet includes fiber bundles having a bundle width of 50 ?m or greater and opened fibers ranging from fiber bundles having a bundle width less than 50 ?m to fibers obtained by opening to the single-fiber level. When the direction along which the opened fibers have been oriented most is a 0° direction and the range of from the 0° to 90° direction is divided into angular zones, the distribution curve showing the proportion of the number of fiber bundles in each angular zone to that in all angular zones and the distribution curve showing the proportion of the number of opened fibers in each angular zone to that in all angular zones are reverse to each other in terms of gradient of the 0° to 90° direction.

Abstract: There are numerous proteins having different in vivo effects depending on variants. In order to gain a more accurate understanding of an in vivo effect of a protein, when the protein in a sample is quantified, it is necessary to measure an amount of each variant that can be present. Provided is a method for performing parallel quantification of variants of a protein having 2 or more variants using mass spectrometry. The method includes: protease-digesting a protein in a sample; detecting, using mass spectrometry, among peptides obtained by the protease digestion, peptides having amino acid sequences specific to the variants without separating the peptides; and determining amounts of the variants in the sample based on results of the detection.

Abstract: A method includes receiving an input of a departure point, an arrival point, and a desired arrival time of a specific vehicle to be operated, extracting, from a plurality of route information, a part of route information corresponding to routes that departed from or passed through around the departure point, and arrived at or passed through around the arrival point within a certain time range of the desired arrival time, classifying the part of route information into a plurality of route groups by comparing each other, counting a number of route information classified in each route groups, calculating, for each route groups, a ratio of the number of route information classified in each route groups to total number of the part of route information, and outputting operation information based on the ratio, the operation information including at least one of specific route as candidates for the specific vehicle.

Abstract: A non-transitory computer-readable recording medium has stored therein a program for finding a point to be improved on a road, the program causing a s computer to execute a process including receiving driving information from a plurality of vehicles, the driving information including information on a position, a time, and a speed, and finding a road section based on the driving information of a first group of vehicles and the driving information of a second group of vehicles in a predetermined area and a predetermined period, the road section being a road section in which the speed of the vehicles in the second group of vehicles is affected by the vehicles in the first group of vehicles.

Abstract: The present invention provides a method for quantifying a monoclonal antibody, the method including: a step of performing selective protease digestion of a monoclonal antibody as a measurement target by bringing a porous body in which the monoclonal antibody is immobilized in pores into contact with fine particles onto which a specific protease is immobilized; and a step of detecting a peptide fragment that is obtained from the digestion and contains an amino acid derived from a CDR2 region of a heavy chain or a light chain of the monoclonal antibody.

Abstract: A non-transitory computer-readable recording medium has stored therein a road state management program for causing a computer to execute a process, the process including receiving at least one information selected from the group including of information on a state of a surface of a road and information on a state under a surface of the road, distributing the received information to one or more management units of the road, and extracting the plurality of pieces of the distributed information so as to be in order of time, wherein each of the distributed information is common place of the road.

Abstract: A method is provided for more easily detecting and quantifying a protein by regioselectively digesting a variable region of an Fab domain of a monoclonal antibody while suppressing proteolysis of an Fc domain. In the method, a porous body in which a monoclonal antibody is immobilized in pores and nanoparticles on which a protease is immobilized are brought into contact with each other in a liquid to perform selective proteolysis of the monoclonal antibody, and a resulting peptide fragment is detected using liquid chromatography-mass spectrometry (LC-MS), and a peptide having an amino acid sequence that includes an amino acid derived from a CDR2 region of a heavy chain or a light chain of the monoclonal antibody is detected.