Abstract: The present invention provides a highly active protease that can be used in sample preparation for mass spectrometry of a protein and has excellent stability against a change in an external environment. The present invention provides an immobilized protease characterized in that a crudely purified protease or a protease that has not been subjected to a self-digestion resistance treatment is immobilized on surfaces of nanoparticles, and provides a method for producing the immobilized protease. The immobilized protease of the present invention can maintain high activity without being subjected to a change in an external environment, and thus is effective, for example, in preparing a sample to be supplied for mass spectrometry of a protein in a clinical specimen.

Abstract: A sample preparation kit related to the present invention provides a significantly versatile analytical technique that is not affected by the diversity of antibodies, difference in species, matrix and the like. For preparing a sample to be used for detection of a monoclonal antibody through high-performance liquid chromatography-mass spectrometry (LC-MS), the kit includes a porous body for immobilizing a monoclonal antibody to be detected; nanoparticles with an immobilized protease; a reaction vessel for selectively digesting the monoclonal antibody by bringing the porous body and nanoparticles into contact; a buffer to be introduced into the reaction vessel along with the nanoparticles and porous body so that a protease reaction is carried out; and a filtration membrane to remove the porous body and nanoparticles after the proteolysis so as to extract the reaction product and the buffer.

Abstract: The present invention provides: a method for increasing a yield, from a target monoclonal antibody, of a peptide fragment that comprises an Fab region or a variable region of the antibody or a portion of the region, the method being characterized by including a process in which digestion of the target monoclonal body is performed by bringing the target monoclonal antibody, which is immobilized on inner surfaces of pores of a porous body, and a protease, which is immobilized on surfaces of nanoparticles having an average particle size larger than an average pore diameter of the porous body, into contact with each other in a liquid by stirring or tapping rotation stirring at a low speed of less than 100 rpm so as to maintain a uniform dispersion, the average pore diameter of the porous body being in a range of 10 nm-200 nm and the average particle size of the nanoparticles being in a range of 50 nm-500 nm; and a kit for use in the method.

Abstract: A server device acquires a monitoring result of a specific type of vital sign of a specific user and identification information of a first monitoring device from the first monitoring device that monitors the specific type of vital sign. The server device acquires a monitoring result of a specific type of vital sign of the specific user and identification information of a second monitoring device from the second monitoring device that monitors the specific type of vital sign. For a time series fluctuation of the specific type of vital sign of the specific user, when a combination of the monitoring result of the first monitoring device and the monitoring result of the second monitoring device is to be output, the server device outputs the monitoring result of the first monitoring device and the monitoring result of the second monitoring device in a distinguishable manner.

Abstract: A carbon fiber composite material includes the material is made of carbon fibers and a thermoplastic resin; the proportion Y, relative to the total weight of carbon fibers, of carbon fiber bundles for which Mn/(Ln×D) is 8.5×10?1 (mg/mm2) or more, is 30?Y<90 (wt %); the average value X of Mn/Ln for the carbon fiber bundles is 1.1×10?2?X?8.1×10?2 (mg/mm); and Y satisfies Y?100X+30, wherein Mn: weight of carbon fiber bundles, Ln: fiber length of carbon fibers, D: fiber diameter of carbon fibers. The carbon fiber composite material combines high flowability and mechanical properties, has few variations in mechanical properties and shows an excellent adaptability of carbon fibers for small parts such as ribs.

Abstract: A non-transitory, computer-readable recording medium stores therein a trigger condition determining program that causes a computer to execute a process including distributing and allocating to plural vehicle groups, a pattern candidate when plural pattern candidates of a trigger condition for control in a vehicle corresponding to driving operation is present, the pattern candidate being each pattern candidate of the plural pattern candidates; evaluating the trigger condition corresponding to the allocated pattern candidate, based on a change in travel information before and after an application of the trigger condition corresponding to the allocated pattern candidate; and setting among plural trigger conditions, a trigger condition having a relatively high evaluation or satisfying a predetermined standard to be a trigger condition that is to be applied in a service provided to the plural vehicle groups.

Abstract: A non-transitory computer-readable recording medium stores a driving support program that causes a computer to execute a process including: collecting vital sign information on a user from a vital sign measuring device; generating a drowsiness occurrence time pattern with respect to the user based on the collected vital sign information; and in response to a request in which a driver is specified from a source of the request, providing the drowsiness occurrence time pattern that is generated with respect to the user corresponding to the driver to the source of the request to the source of request or providing alerting information to the driver that is determined according to the drowsiness occurrence time pattern generated with respect to the user corresponding to the driver to the source of request.

Abstract: A server device receives an input including identification information of a specific driver. The server device specifies, with reference to a storage storing a service period in a specific company and travel data during the service period of the specific driver, the travel data during the service period corresponding to the specific driver. Moreover, the server device acquires a traveling feature during the service period of the specific driver on the basis of the specified travel data so as to output the traveling feature. As a result, it is possible to output the traveling feature of the specific driver.

Abstract: A measurement instrument includes: a processor configured to: receive registration of user identification information and a plurality of transmission destinations; and transmit a result of measurement that concerns health management measured by the measurement instrument to each of the transmission destinations registered in association with the registered user identification information.

Abstract: A method includes receiving an input of a departure point, an arrival point, and a desired arrival time of a specific vehicle to be operated, extracting, from a plurality of route information, a part of route information corresponding to routes that departed from or passed through around the departure point, and arrived at or passed through around the arrival point within a certain time range of the desired arrival time, classifying the part of route information into a plurality of route groups by comparing each other, counting a number of route information classified in each route groups, calculating, for each route groups, a ratio of the number of route information classified in each route groups to total number of the part of route information, and outputting operation information based on the ratio, the operation information including at least one of specific route as candidates for the specific vehicle.

Abstract: A non-transitory computer-readable recording medium stores a work managing program that causes a computer to execute a process including: specifying, with reference to a memory that memorizes implementation plans of works for maintenances of a road and/or implementation plans of works for maintenances of objects buried in undergrounds of the road in association with places in which the respective works are to be implemented, a place for which both one of the works for maintenances of the road and one of the works for maintenances of objects are planned; and displaying implementation plans of the one of the works for maintenances of the road and the one of the works for maintenances of objects buried in undergrounds of the road corresponding to the specified place so that the implementation plans of both the works are able to be distinguished from implementation plans of other works.

Abstract: A travel path analysis support device includes a processor that executes a procedure. The procedure includes: performing, in accordance with a selected first display color control, change control of display colors of respective regions obtained by mesh-dividing within the geographic region according to a time-wise change in a number of vehicles that pass through the respective regions; when an instruction for enlarged display of a partial region inside the geographic region is received at as given timing of a procedure by which display colors of the respective regions are changed by the change control, displaying the partial region enlarged, and selecting a second display color control based on a total number of vehicles that have entered the partial region within the time range since the given timing; and performing, in accordance with the selected second display color control, change control of the display colors of the respective regions.

Abstract: An electric brake device includes: a motor cylinder device that generates a hydraulic pressure in accordance with a brake operation of a driver with an electric motor; a rotational angle sensor that detects a rotor rotational angle ? of the electric motor; and a motor control section that controls an output torque of the electric motor corresponding to the rotor rotational angle ? and controls the electric motor by advancing a detection value of the rotational angle sensor. The current phase setup section sets a current phase to an advanced side as an electrical advance control. The current phase setup section inhibits the advancing the detection value when the electric motor performs reversed rotation, or sets the current phase to a retarded side.

Abstract: A negative electrode 1 for lithium secondary batteries, which can increase the charge/discharge capacity of a lithium secondary battery, includes a negative electrode current collector, a negative electrode active material layer, and a lithium layer. The negative electrode active material layer is disposed on regions and of the respective surfaces and of the negative electrode current collector. The lithium layer is disposed on uncovered regions and, which are regions of the respective surfaces and of the negative electrode current collector on which the negative electrode active material layer is not disposed. The lithium layer includes lithium.

Abstract: There is provided a brake system for vehicles, the brake system including: a slave cylinder S/C to generate a hydraulic pressure by an electric motor according to a braking operation by a driver; and a controller to decrease an operation amount of the slave cylinder S/C when an operation amount of the electric motor is maintained, then increase an operation amount of the slave cylinder S/C when a change occurs in an operation amount of the electric motor subsequently. The controller determines whether or not the phase currents Iu, Iv, Iw of the electric motor are in two-phase continuously energized state and the state remains for a certain time or longer. When the determination conditions are satisfied, the controller performs commutation control to generate commutation in the electric motor by reducing a target hydraulic pressure of the slave cylinder S/C.

Abstract: The vehicle braking device includes a hydraulic pressure generating device generating a primary hydraulic pressure corresponding to the braking operation by a driver, a motor cylinder device operating to cause the secondary hydraulic pressure generated by operation of the electric motor to track the target hydraulic pressure based on the control operation quantity, a brake pedal sensor detecting the braking operation quantity, a primary hydraulic pressure sensor, an ECU performing switching control in accordance with whether the braking operation quantity detected by the brake pedal sensor reaches a predetermined threshold value, between operating the motor cylinder device on the basis of the braking operation quantity and operating the motor cylinder device on the basis of a correlation value of the braking operation force detected by the motor cylinder device 16 or the correlation value and the braking operation quantity.

Abstract: In a method of measuring a road state, the method includes extracting, based on measurement values of an acceleration sensor obtained when a vehicle having the acceleration sensor installed therein travels a predetermined road section, a measurement target section in which an MCI value is to be measured, the measurement target section being a part of the predetermined road section and including a road surface that meets a predetermined degradation criteria; and limiting, to the measurement target section that is the part of the predetermined road section, a measurement target in which the MCI value is to be measured using a road surface condition measurement vehicle having laser scanning and camera image capturing functions.

Abstract: A stacked plate for window includes a laminated plate that includes a first transparent plate, a second transparent plate that faces the first transparent plate, and an intermediate film that bonds the first transparent plate to the second transparent plate; a conductive layer, at least a part of which is provided between the first transparent plate and the second transparent plate; and an insulating sheet that retains the conductive layer, the insulating sheet being bonded to the intermediate film between the first transparent plate and the second transparent plate.

Abstract: A travel path analysis support device includes a processor that executes a procedure. The procedure includes: performing, in accordance with a selected first display color control, change control of display colors of respective regions obtained by mesh-dividing within the geographic region according to a time-wise change in a number of vehicles that pass through the respective regions; when an instruction for enlarged display of a partial region inside the geographic region is received at as given timing of a procedure by which display colors of the respective regions are changed by the change control, displaying the partial region enlarged, and selecting a second display color control based on a total number of vehicles that have entered the partial region within the time range since the given timing; and performing, in accordance with the selected second display color control, change control of the display colors of the respective regions.

Abstract: Provided is a brake system for a vehicle including an input side hydraulic system, an output side hydraulic system, a first and a second shutoff valves to shut off flowing of the brake fluid between the input side hydraulic system and the output side hydraulic system when closed, a first and a second out-valves through which the brake fluid flows into a reservoir unit when opened, and a control unit to control a motor cylinder device of the output side hydraulic system to generate the brake fluid pressure according to the operational amount of a brake pedal. The control unit opens one or both of the first and the second shutoff valves and the first and the second out-valves when output characteristic of the brake fluid pressure generated by the motor cylinder device shifts from basic characteristic.