Patents by Inventor Thomas H. Turpen
Thomas H. Turpen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7192740Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: GrantFiled: October 24, 2002Date of Patent: March 20, 2007Assignee: Large Scale Biology CorporationInventors: Jonathan Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Jr., Laurence K. Grill
-
Patent number: 7084256Abstract: A polypeptide self-antigen useful in a tumor-specific vaccine mimics one or more epitopes of an antigen uniquely expressed by cells of the tumor. The polypeptide is preferably produced in a plant that has been transformed or transfected with nucleic acid encoding the polypeptide and is obtainable from the plant in correctly folded, preferably soluble form without a need for denaturation and renaturation. This plant-produced polypeptide is immunogenic without a need for exogenous adjuvants or other immunostimulatory materials. The polypeptide is preferably an scFv molecule that bears the idiotype of the surface immunoglobulin of a non-Hodgkin's (or B cell) lymphoma. Upon administration to a subject with lymphoma, the plant-produced, tumor-unique scFv polypeptide induces an idiotype-specific antibody or cell-mediated immune response against the lymphoma.Type: GrantFiled: February 8, 2002Date of Patent: August 1, 2006Assignee: Large Scale Biology CorporationInventors: Alison A. McCormick, Daniel Tusé, Stephen J. Reinl, John A. Lindbo, Thomas H. Turpen
-
Patent number: 7033835Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.Type: GrantFiled: April 7, 1998Date of Patent: April 25, 2006Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Reinl, Laurence K. Grill
-
Patent number: 7034128Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: October 29, 2004Date of Patent: April 25, 2006Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
-
Patent number: 6890748Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: March 20, 2002Date of Patent: May 10, 2005Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
-
Patent number: 6887696Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: November 13, 2001Date of Patent: May 3, 2005Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
-
Patent number: 6852846Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus, the recombinant transcripts are replicated producing high levels of foreign gene RNA.Type: GrantFiled: August 14, 2001Date of Patent: February 8, 2005Assignee: Large Scale Biology CorporationInventor: Thomas H. Turpen
-
Patent number: 6846968Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: July 26, 2000Date of Patent: January 25, 2005Assignee: Large Scale Biology CorporationInventors: Robert L. Erwin, Laurence K. Grill, Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai
-
Patent number: 6841659Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: August 1, 2003Date of Patent: January 11, 2005Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
-
Publication number: 20040234516Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human a galactosidase nucleotide sequences.Type: ApplicationFiled: May 21, 2004Publication date: November 25, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
-
Publication number: 20040175694Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.Type: ApplicationFiled: August 20, 2003Publication date: September 9, 2004Applicant: Biosource Technologies, Inc.Inventors: Thomas H. Turpen, Stephen J, Reinl, Laurence K. Grill
-
Publication number: 20040171813Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art.Type: ApplicationFiled: April 20, 2004Publication date: September 2, 2004Inventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
-
Patent number: 6740740Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a larger scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target protein/peptide from other components of the green juice by one or more cycles of centrifugation and/or resuspension, and finally purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: September 24, 2001Date of Patent: May 25, 2004Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
-
Publication number: 20040093646Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: October 9, 2003Publication date: May 13, 2004Inventors: Robert L. Erwin, Laurence K. Grill, Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai
-
Publication number: 20040064855Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: October 9, 2003Publication date: April 1, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai, Robert L. Erwin, Laurence K. Grill
-
Publication number: 20040049025Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: ApplicationFiled: October 24, 2002Publication date: March 11, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Jon Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill
-
Publication number: 20040047923Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: ApplicationFiled: August 1, 2003Publication date: March 11, 2004Inventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
-
Publication number: 20040023281Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: June 23, 2003Publication date: February 5, 2004Inventors: Thomas H. Turpen, Monto H. Kumagai, Gregory P. Pogue, Robert L. Erwin, Laurence K. Grill
-
Publication number: 20040016021Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: June 23, 2003Publication date: January 22, 2004Inventors: Thomas H. Turpen, Gregory P. Pogue, Robert L. Erwin, Laurence K. Grill
-
Patent number: 6660500Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.Type: GrantFiled: January 5, 2001Date of Patent: December 9, 2003Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Reinl, Laurence K. Grill