Patents by Inventor Thomas H. Turpen
Thomas H. Turpen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6303779Abstract: The present invention features a method for isolating and purifying vitamins and sugars from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating vitamins and sugars than those methods described in the prior art. In general, the present method of isolating vitamins and sugars comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either vitamins or sugars, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying vitamins or sugars by such procedure as PEG-precipitation, chromatography and/or salt precipitation.Type: GrantFiled: December 17, 1999Date of Patent: October 16, 2001Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Patent number: 6300134Abstract: This invention is directed to a plus strand RNA viral vector for transformation of a host organism with a foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing cis-acting viral replication elements, and allowed to infect the host organism. The RNA vector is modified to obtain infectivity by including an intervening sequence between the cap and the 5′ end. The modified RNA is able to tolerate the exogeneous RNA segment without disrupting the replication of the modified RNA, in the absence of a trans-acting viral replication element in a single component plant virus host cell.Type: GrantFiled: February 11, 2000Date of Patent: October 9, 2001Assignee: Large Scale Biology CorporationInventors: John A. Lindbo, Gregory P. Pogue, Thomas H. Turpen
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Patent number: 6300133Abstract: This invention is directed to a plus strand RNA viral vector for transformation of a host organism with a foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing cis-acting viral replication elements, and allowed to infect the host organism. The RNA vector is modified to obtain infectivity by not incorporating a cap at the 5′ end of the genome. The modified RNA is able to tolerate the exogenous RNA segment without disrupting the replication of the modified RNA, in the absence of a trans-acting viral replication element in a single component plant virus host cell.Type: GrantFiled: February 11, 2000Date of Patent: October 9, 2001Assignee: Large Scale Biology CorporationInventors: John A. Lindbo, Gregory P. Pogue, Thomas H. Turpen
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Patent number: 6284492Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: GrantFiled: June 7, 1995Date of Patent: September 4, 2001Assignee: Large Scale Biology CorporationInventors: Jon Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann M. Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 6284875Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: February 9, 2000Date of Patent: September 4, 2001Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6054566Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: GrantFiled: June 7, 1995Date of Patent: April 25, 2000Assignee: Biosource Technologies, Inc.Inventors: Jon Donson, William O. Dawson, George L. Granthan, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 6037456Abstract: The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspenion, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: March 10, 1998Date of Patent: March 14, 2000Assignee: Biosource Technologies, Inc.Inventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Patent number: 6033895Abstract: The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: February 25, 1999Date of Patent: March 7, 2000Assignee: Biosource Technologies, Inc.Inventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Patent number: 5977438Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.Type: GrantFiled: October 14, 1994Date of Patent: November 2, 1999Assignee: Biosource Technologies, Inc.Inventors: Thomas H. Turpen, Stephen J. Reinl, Laurence K. Grill
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Patent number: 5965794Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.Type: GrantFiled: November 9, 1994Date of Patent: October 12, 1999Assignee: Biosource Technologies, Inc.Inventor: Thomas H. Turpen
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Patent number: 5889191Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.Type: GrantFiled: June 7, 1995Date of Patent: March 30, 1999Assignee: Biosource Technologies, Inc.Inventor: Thomas H. Turpen
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Patent number: 5889190Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.Type: GrantFiled: June 7, 1995Date of Patent: March 30, 1999Assignee: Biosource Technologies, Inc.Inventors: Jon Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 5866785Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.Type: GrantFiled: June 7, 1995Date of Patent: February 2, 1999Assignee: Biosource Technologies, Inc.Inventors: Jon Donson, William O. Dawson, George L. Granthan, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 5837505Abstract: The present invention is directed to a process for producing melanins, their precursors and their analoges, hereinafter referred to generically as melanins. According to the invention, melanins are produced in amounts greater than about 3.3 grams wet weight per liter of growth medium. The enhanced production of melanin can be achieved by manipulating the constituents of the growth medium, and/or attenuating fermentations conditions, and/or by genetically engineering microorganisms to produce melanins, and/or mutating the microorganisms.Type: GrantFiled: March 9, 1995Date of Patent: November 17, 1998Assignee: Biosource Technologies, Inc.Inventors: Guy della-Cioppa, Stephen J. Garger, Jr., Genadie G. Sverlow, Thomas H. Turpen, Laurence K. Grill, Miles R. Chedekal
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Patent number: 5814495Abstract: The present invention is directed to a process for producing melanins, their precursors and their analoges, hereinafter referred to generically as melanins. According to the invention, melanins are produced in amounts greater than about 3.3 grams wet weight per liter of growth medium. The enhanced production of melanin can be achieved by manipulating the constituents of the growth medium, and/or attenuating fermentations conditions, and/or by genetically engineering microorganisms to produce melanins, and/or mutating the microorganisms.Type: GrantFiled: March 14, 1995Date of Patent: September 29, 1998Assignee: Biosource Technologies, Inc.Inventors: Guy della-Cioppa, Stephen J. Garger, Jr., Genadie G. Sverlow, Thomas H. Turpen, Laurence K. Grill, Miles R. Chedekal
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Patent number: 5811653Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.Type: GrantFiled: December 29, 1993Date of Patent: September 22, 1998Assignee: Biosource Technologies, Inc.Inventor: Thomas H. Turpen
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Patent number: 5631151Abstract: The present invention is directed to a process for producing melanins, their precursors and their analogues, hereinafter referred to generically as melanins. According to the invention, melanins are produced in amounts greater than about 3.3 grams wet weight per liter of growth medium. The enhanced production of melanin can be achieved by manipulating the constituents of the growth medium, and/or attenuating fermentations conditions, and/or by genetically engineering microorganisms to produce melanins, and/or mutating the microorganisms.Type: GrantFiled: March 20, 1992Date of Patent: May 20, 1997Assignee: Biosource Technologies, Inc.Inventors: Guy della-Cioppa, Stephen J. Garger, Jr., Genadie G. Sverlow, Thomas H. Turpen, Laurence K. Grill, Miles R. Chedekel, Monto H. Kumagai
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Patent number: 5589367Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.Type: GrantFiled: January 19, 1994Date of Patent: December 31, 1996Assignee: Biosource Technologies, Inc.Inventors: Jon Donson, William O. Dawson, George L. Granthan, Thomas H. Turpen, Ann M. Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 5316931Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.Type: GrantFiled: July 31, 1992Date of Patent: May 31, 1994Assignee: Biosource Genetics Corp.Inventors: Jon Donson, William O. Dawson, George L. Granthan, Thomas H. Turpen, Ann M. Turpen, Stephen J. Garger, Laurence K. Grill