Abstract: The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.

Abstract: Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: A method for eliminating acoustic reverberation in an audio signal. First and second level measurements are performed on the audio signal. The first level measurement uses a first attack parameter and a first decay parameter to measure a first attack time and a first decay time. The second level measurement uses a second attack parameter and a second decay parameter to form a second attack time that is identical to the first attack time and a second decay time that is longer than the first decay time. A difference between the first and second level measurements is calculated. The difference and the second level measurement are used to estimate a reverberation interference level, and the first level measurement and the reverberation interference level are used to ascertain a gain parameter for the audio signal.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: Techniques for determining personalized head-related transfer functions (HRTFs) using a head-mounted device and in-ear devices include: receiving, from a sensor array of the head-mounted device, a first sound signal associated with a sound from a sound source in a local environment of a user of the head-mounted device; determining that reverberation characteristics and spectral characteristics of the sound meet predetermined criteria based on the first sound signal; determining that the sound source is stationary within a time period; determining a relative location of the sound source with respect to the user; receiving, from an in-ear device in an ear of the user, a second sound signal associated with the sound from the sound source; and determining, based on at least the second sound signal, an HRTF or one or more parameters of the HRTF associated with the relative location of the sound source for the user.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A method for directional signal processing of signals of a microphone array, including first and second microphones generating first and second input signals from an ambient sound, uses the first input signal to form a reference signal transformed into the frequency domain, thereby generating a frequency-space reference signal. The first and second input signals are transformed into the frequency domain, and a first frequency-space directional signal is formed in the frequency domain using the transformed first and second input signals. A frequency-resolved comparison of the frequency-space reference signal with the first frequency-space directional signal or signal derived therefrom in the frequency domain, is used to generate frequency-dependent first gain factors to generate a time filter in the time domain. The reference signal is filtered by the time filter and an output signal is generated from the reference signal filtered by the time filter.

Abstract: A method includes receiving an image of a plurality of fiducial markers, where the plurality of fiducial markers has at least one predetermined height relative to a substrate. The method also includes determining at least one optical metric associated with the plurality of fiducial markers within the image, and determining a calibration model based on the at least one predetermined height and the at least one optical metric.

Abstract: A method for processing an input audio signal includes using a first analytical filter bank to divide the input audio signal in a first frequency splitting process into a plurality of first frequency bands. The first frequency bands of a first subgroup are divided in a further frequency splitting process by a further analytical filter bank into a plurality of frequency subbands. The divided input audio signal is frequency-selectively processed or amplified. The divided and processed input audio signal is then combined again into an output audio signal. A prediction is applied to the first frequency bands of the first subgroup and/or the frequency subbands derived therefrom, to compensate for latency differences between the first frequency bands and the frequency subbands as a result of the or each further frequency splitting process. A device or hearing aid for carrying out the method is also provided.

Abstract: A method performs directional signal processing for a hearing instrument. First and second input signals are generated by first and second input transducers, respectively, from a sound signal. The first front intermediate signal and a first rear intermediate signal are each formed from the first and second input signals. A first superposition of the first front intermediate signal and the first rear intermediate signal is formed by a complex-value first superposition parameter and is adapted based on the first superposition parameter. A complex value of the first superposition parameter resulting from the adaptation of the first superposition is converted into a first alternative parameter and a second alternative parameter. An output signal is generated based on the first alternative parameter, the limited second alternative parameter and a superposition of the first and second input signals.

Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Abstract: A method for operating a hearing device. In this method, an entire audio signal is detected by means of a microphone. The entire audio signal is divided into a first audio signal and a second audio signal. A speech intelligibility of the second audio signal is reduced. The first audio signal and the second audio signal are combined to form an output signal, and the output signal is output by means of an output device. Further, a hearing device is provided.