Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications, including cell lineage analysis. Cell lineage analysis may comprise the use of one or more lineage tracing nucleic acid molecules. The disclosed methods may comprise using a lineage tracing nucleic acid molecule to identify a biological particle with one or more progenitor cells.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including generation of labeled macromolecules, including major mistocompatability complex (MHC) molecules, dextramers, etc. Labeled macromolecules may be generated using an in vitro transcription reaction. Labeled macromolecules may be generated in one or more partitions.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A method for detecting a defect in a hearing instrument that has at least one first input transducer and at least one output transducer. A first transfer function of a first acoustic system, which includes the output transducer and the first input transducer, is determined, and at least a first reference function for the first transfer function is determined. The first transfer function is compared with the first reference function and a defect in the hearing instrument is detected based on the comparison. A hearing instrument with an input transducer and an output transducer is set up to carry out the method.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: A method for the frequency distortion of an audio signal includes splitting the audio signal into a plurality of specified frequency bands, in which a band limit frequency is defined in each case by two respective immediately adjacent frequency bands. A first frequency band and a second frequency band lying immediately above the first frequency band are determined on the basis of the audio signal. A distortion of the frequencies differing from the distortion applied to signal components in the second frequency band is applied to signal components in the first frequency band, and a frequency-distorted signal is generated as a result. A method for suppressing an acoustic feedback in an acoustic system and a hearing aid are also provided.

Abstract: A method and an apparatus reduce feedback in a hearing aid device. The method includes the step of acquiring a first feedback transfer function at a first point in time on a feedback path from a signal processing device via an electro-acoustic transducer, an acoustic signal path from the electro-acoustic transducer to an acousto-electric transducer and via the acousto-electric transducer back to the signal processing device. In a further step, a weighted mean value function is determined in a manner dependent on amplitude absolute values of the first feedback transfer function. A second feedback transfer function is estimated by an adaptive filter, wherein coefficients of the adaptive filter are determined in a manner dependent on the weighted mean value function. The adaptive filter is applied to a signal which is derived from an acoustic input signal of the acousto-electric transducer.

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Abstract: A method suppresses feedback in a hearing instrument. A microphone generates an input signal and a loudspeaker generates an acoustic signal which is partially fed back to the microphone via an acoustic feedback path. An intermediate signal is generated along a main signal path depending on the input signal, and an output signal is formed on the basis of the intermediate signal. A voice activity of a user is monitored, a transfer function of an electro-acoustic closed signal loop formed by the main signal path and the feedback path is estimated, that, depending on the transfer function of the closed signal loop and the voice activity of the user, the intermediate signal is decorrelated to form the output signal. A compensation signal is generated from the output signal and is fed to the input signal for feedback compensation, and the output signal is fed to the loudspeaker for reproduction.

Abstract: A microfluidic system for manipulating particles in a fluid is provided. The system includes a microfluidic chip having at least one channel and at least one filter feature, wherein the filter feature includes a field of physical obstacles configured and arranged to remove physical contaminants associated with the particles. The filter features are particularly suited to remove contaminants associated with gel beads manipulated by microfluidic handling.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.