Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: A method performs directional signal processing for a hearing aid. First and second input transducers of the hearing aid generate first and second input signals, respectively, from a sound signal. A first calibration directional signal which has a relative attenuation in the direction of a first useful signal source is generated from the first and second input signals, and a second calibration directional signal which has a relative attenuation in the direction of a second useful signal source is generated from the first and second input signals. A relative gain parameter is determined from the first and second calibration directional signals. First and second processing directional signals are generated from both the first and second input signals. A source-sensitive directional signal is generated from the first and second processing directional signals and the relative gain parameter. An output signal of the hearing aid is generated from the source-sensitive directional signal.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A method reduces noise in an audio signal. In the method a signal component subsequent to the prediction time is predicted for a plurality of prediction times with reference to signal components of the audio signal that are respectively prior to the prediction time. A predicted audio signal is formed from the signal components respectively following a prediction time, and a noise-reduced audio signal is generated based on the predicted audio signal.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Abstract: A method of suppressing an acoustic reverberation in an audio signal includes providing an audio signal, carrying out a first level measurement of the audio signal and carrying out a second level measurement of the audio signal at least temporarily during the first level measurement. The first level measurement is carried out with a decay time and/or adjustment time that differs from a decay time or adjustment time of the second level measurement. An acoustic reverberation of the sound event in the audio signal is suppressed by attenuating the audio signal in response to a sound event in the audio signal. The attenuation is controlled as a function of a difference between the first level measurement and the second level measurement. A hearing device is also provided.

Abstract: The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Abstract: A method distorts the frequency of an input signal that is present as an audio signal. Here, the input signal is divided into a low-frequency signal component and a high-frequency signal component. These two signal components adjoin one another at a cut-off frequency. The high-frequency signal component is frequency-distorted and overlaid with the low-frequency signal component to form an output signal. An associated gain factor is modified, at least for an edge region, containing the cut-off frequency, of the high-frequency signal component and/or of the low-frequency signal component, such that a level difference between a signal level of the low-frequency signal component and a signal level of the frequency-distorted high-frequency signal component is increased.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: A method performs frequency distortion of an audio signal. The audio signal is divided at at least one division frequency into a low-frequency band and a high-frequency band. A frequency-distorted signal is generated through respectively different distortions of frequencies for the high-frequency band and for the low-frequency band. The division frequency is selected such that it is located between two neighboring tones of a given tonal system.

Abstract: A method operates a hearing aid which has at least one input transducer and at least one output transducer. An input signal is generated by the at least one input transducer from a sound signal in the environment. From the input signal, a classification of a hearing situation of the environment is determined and/or at least one of four parameters including tonality, loudness, stationarity and reverberation time is determined for the sound signal of the environment. A first intermediate signal is generated in dependence on the input signal by signal processing. Wherein by the classification of the hearing situation and by at least one of the four parameters of tonality, loudness, stationarity and reverberation time, at least one parameter of a frequency distortion is predetermined. The frequency distortion predetermined in this way is applied to the first intermediate signal.