Abstract: The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

Abstract: An observation apparatus observes an observation object moving in a flow path with a fluid, and includes a light source, a splitting unit, a combining unit, a collimator, a cylindrical lens, an objective lens, a collimator, a cylindrical lens, an objective lens, a modulation unit, an imaging unit, an analysis unit, and the like. The imaging unit includes a plurality of pixels arranged in a direction intersecting with a moving direction of an image of the observation object on a light receiving plane on which the image of the observation object moving in the flow path is formed, and receives combined light output from the combining unit to repeatedly output a detection signal indicating a one-dimensional interference image. The analysis unit generates a two-dimensional image of the observation object on the basis of the detection signal.

Abstract: An interference observation apparatus includes a light source, a splitting beam splitter, a combining beam splitter, a beam splitter, a mirror, a beam splitter, a mirror, a piezo element, a stage, an imaging unit, an image acquisition unit, and a control unit. An interference optical system from the splitting beam splitter to the combining beam splitter forms a Mach-Zehnder interferometer. The mirror freely moves in a direction perpendicular to a reflecting surface of the mirror. The total number of times of respective reflections of first split light and second split light in the interference optical system is an even number.

Abstract: The identification apparatus includes a quantitative phase image acquisition unit, a feature quantity extraction unit, a learning unit, a storage unit, and an identification unit. The feature quantity extraction unit extracts a feature quantity of a quantitative phase image of a cell. The learning unit performs machine learning for a quantitative phase image of a known cell of which a type is known based on the feature quantity extracted by the extraction unit. The storage unit stores a result of the machine learning by the learning unit. The identification unit determines, based on the feature quantity extracted by the extraction unit for the quantitative phase image of an unknown cell of which a type is unknown, the type of the unknown cell using the learning result stored by the storage unit.

Abstract: A cell stimulation method includes continuously emitting mid-infrared light to a living cell and thus changing an ion concentration of the cell or changing ion concentrations of the cell and other cells disposed around the cell.

Abstract: A spectroscopic measurement apparatus includes a light source, a diffraction grating being a spectroscopic unit, a spatial filter unit, a detection unit, and an analysis unit. The diffraction grating spatially disperses light from the light source, and outputs the light to different optical paths according to a wavelength. The spatial filter unit inputs the light from the diffraction grating to different positions according to the wavelength, applies loss depending on the wavelength to the light, and outputs the light. The detection unit detects the intensity of the light from the spatial filter unit. The analysis unit obtains the intensities of light in an absorption band and light in a non-absorption band of a component in a measurement sample on an optical path between the light source and the detection unit based on the detection result, and evaluates the component in the measurement sample.

Abstract: An interference observation apparatus includes a light source which outputs incoherent light, a beam splitter, a sample holding table, an objective lens, a reference mirror, a lens, an aberration correction plate, a piezo element, a tube lens, a beam splitter, an imaging unit, a photodetector, an image acquisition unit, and a control unit. The control unit obtains an interference intensity of combined light on the basis of a detection signal output from the photodetector, and adjusts an interference optical system to increase the interference intensity.

Abstract: An interference observation apparatus includes an interference optical apparatus , a microscope housing, an imaging unit, and an objective lens. The interference optical apparatus includes a housing, a light source, a photodetector, beam splitters, a reference mirror, and a control unit. The interference observation apparatus is configured such that the housing of the interference optical apparatus is disposed between the objective lens attachment portion and the objective lens in a microscope apparatus including the imaging unit for capturing an image of the light passing through the objective lens attached to the objective lens attachment portion having an opening.

Abstract: An interference observation apparatus includes a light source, a splitting beam splitter, a combining beam splitter, a beam splitter, a mirror, a beam splitter, a mirror, a piezo element, a stage, an imaging unit, an image acquisition unit, and a control unit. An interference optical system from the splitting beam splitter to the combining beam splitter forms a Mach-Zehnder interferometer. The mirror freely moves in a direction perpendicular to a reflecting surface of the mirror. The total number of times of respective reflections of first split light and second split light in the interference optical system is an even number.

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn?. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn? at each time tn.

Abstract: The identification apparatus includes a quantitative phase image acquisition unit, a feature quantity extraction unit, a learning unit, a storage unit, and an identification unit. The feature quantity extraction unit extracts a feature quantity of a quantitative phase image of a cell. The learning unit performs machine learning for a quantitative phase image of a known cell of which a type is known based on the feature quantity extracted by the extraction unit. The storage unit stores a result of the machine learning by the learning unit. The identification unit determines, based on the feature quantity extracted by the extraction unit for the quantitative phase image of an unknown cell of which a type is unknown, the type of the unknown cell using the learning result stored by the storage unit.

Abstract: The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract: A spectroscopic measurement apparatus includes a light source, a diffraction grating being a spectroscopic unit, a spatial filter unit, a detection unit, and an analysis unit. The diffraction grating spatially disperses light from the light source, and outputs the light to different optical paths according to a wavelength. The spatial filter unit inputs the light from the diffraction grating to different positions according to the wavelength, applies loss depending on the wavelength to the light, and outputs the light. The detection unit detects the intensity of the light from the spatial filter unit. The analysis unit obtains the intensities of light in an absorption band and light in a non-absorption band of a component in a measurement sample on an optical path between the light source and the detection unit based on the detection result, and evaluates the component in the measurement sample.

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract: The observation device 1 is a device capable of substantially simultaneously acquiring the fluorescence image and the interference image on the common observation plane in the observation object 90 mounted on slide glass 41, and has an excitation light source 11, a filter 12, a dichroic mirror 13, a lens 14, a dichroic mirror 15, an objective lens 16, a filter 17, a fluorescence imaging unit 18, an interference imaging light source 21, a filter 22, a dichroic mirror 23, a lens 24, a half mirror 25, an objective lens 26, a lens 27, a dichroic mirror 28, an interference imaging unit 29, a position detection light source 31, a light detection unit 32, the slide glass 41, an actuator 42, a reference mirror 43, an actuator 44, a control unit 51, and a display unit 52.

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn?. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn? at each time tn.

Abstract: The present invention relates to a cell evaluation method comprising evaluating a cell differentiation degree based on a cell thickness.

Abstract: The observation device 1 is a device capable of substantially simultaneously acquiring the fluorescence image and the interference image on the common observation plane in the observation object 90 mounted on slide glass 41, and has an excitation light source 11, a filter 12, a dichroic mirror 13, a lens 14, a dichroic mirror 15, an objective lens 16, a filter 17, a fluorescence imaging unit 18, an interference imaging light source 21, a filter 22, a dichroic mirror 23, a lens 24, a half mirror 25, an objective lens 26, a lens 27, a dichroic mirror 28, an interference imaging unit 29, a position detection light source 31, a light detection unit 32, the slide glass 41, an actuator 42, a reference mirror 43, an actuator 44, a control unit 51, and a display unit 52.

Abstract: The observation device 1 is to observe the surface or the inside of an observation object 9, that has light sources 11 and 12, lenses 21 to 25, an aperture 31, an optical multiplexer 41, an optical demultiplexer 42, a half mirror 43, an image pickup unit 51, an analyzing unit 52, a display unit 53, a light receiving unit 61, a displacement detecting unit 62, a piezoelectric actuator 71, a drive unit 72, a mirror 73, a stage 81, a drive unit 82, and a control unit 90. The analyzing unit 52 determines a complex amplitude of an interference light figure taken as an image by the image pickup unit 51 with a phase shift technique after an optical path difference is set to each target value in sequence, and determines an amount of change per certain time of a phase component of a reflected light generated on the surface or the inside of the observation object 9 on the basis of an absolute value of an amount of change per certain time of the determined complex amplitude and an absolute value of the complex amplitude.