Abstract: An automatic locking device for assembling a part to a workpiece is provided. The device includes a feeding mechanism, a fixture mechanism, a loading and unloading mechanism, a lifting mechanism, a rotating mechanism, and a locking mechanism. The feeding mechanism feeds the part to the fixture mechanism. The loading and unloading mechanism places the workpiece on the part. The lifting mechanism lifts up the fixture mechanism together with the part and the workpiece. The rotating mechanism rotates the fixture mechanism. The locking mechanism locks the part to the workpiece after the part and the workpiece are rotated by the rotating mechanism. The automatic locking device integrates multiple functions in a single station, reduces the number of the work stations and operatives, lowers the cost, and improves accuracy of the assembly. An assembling apparatus including the automatic locking device is also provided.

Abstract: The present invention provides a quick-injection grease filling device, which features including an oil cup, a detachable guide seat, a screw, a piston, an oppression part and a rotating drive part. The piston is held inside the oil tank, capable of moving upward and downward. The piston has an oppression surface and a bearing placement surface. The detachable guide seat includes a combination part and a screw hole, and the combination part is attached to the cup mouth through a detachable assembly unit. The inner end of the screw extends into the oil cup, and the outward extending end extends out of the cup mouth. The rotation driving part is arranged on the outward extending end. The oppression part can move forward and backward along with the screw. The oppression part is used to press against the bearing.

Abstract: A quick-injection grease filling device includes an oil cup, a detachable guide seat, a screw, a piston, an oppression part and a rotating drive part. The piston is held inside the oil tank, capable of moving upward and downward. The piston has an oppression surface and a bearing placement surface. The detachable guide seat includes a screw hole and a combination part which is attached to the cup mouth through a detachable assembly unit. The screw is screwed into the screw hole of the detachable guide seat to move forward and backward. The inner end of the screw extends into the oil cup, and the outward extending end extends out of the cup mouth. The rotation driving part is arranged on the outward extending end. The oppression part can move forward and backward along with the screw. The oppression part is used to press against the bearing.

Abstract: The invention relates to a method for discovering specific functional antibodies, in particular to a method for discovering specific functional antibodies based on sequence composition and frequency analysis of variable regions of immune host antibodies. Compared with conventional high-throughput antibody sequencing method, this method can quickly and accurately obtain the full-length sequence of the variable region of antibody containing candidate CDR3, the success rate of antibody gene pairing is high and it is suitable for the detection of few samples, and improves the efficiency of obtaining specific functional antibodies.

Abstract: The lack of blood-brain barrier (BBB) penetrating ability has hindered the delivery of many therapeutic agents for tauopathy therapeutic treatment. A circular bifunctional aptamer reported here has been able to enhance the in vivo BBB penetration for improved therapy. The circular aptamer includes one transferrin receptor (TfR) aptamer to facilitate TfR-aptamer recognition-induced transcytosis across BBB endothelial cells, and one Tau protein aptamer selected to inhibit Tau phosphorylation and other tauopathy-related pathological events in the brain. This bispecific construct exhibits strong specificity towards Tau and enhanced plasma stability in comparison to linear Tau aptamer. In vivo administration of circular Tau-TfR aptamer results in a rapid uptake into relevant brain regions after crossing the BBB, such as hippocampus and cortex.

Abstract: A quick-injection grease filling device includes an oil cup, a detachable guide seat, a screw, a piston, an oppression part and a rotating drive part. The piston is held inside the oil tank, capable of moving upward and downward. The piston has an oppression surface and a bearing placement surface. The detachable guide seat includes a screw hole and a combination part which is attached to the cup mouth through a detachable assembly unit. The screw is screwed into the screw hole of the detachable guide seat to move forward and backward. The inner end of the screw extends into the oil cup, and the outward extending end extends out of the cup mouth. The rotation driving part is arranged on the outward extending end. The oppression part can move forward and backward along with the screw. The oppression part is used to press against the bearing.

Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, can efficiently generate gene knockouts of variably sizes. More precise genome editing, either the insertion or deletion of a desired fragment, can be done by combining the homology-directed-repair (HDR) pathway with CRISPR cleavage. HDR-mediated gene knock-in experiments are inefficient, with no reports of successful gene knock-in with DNA fragments larger than 4 kb. Targeted insertion of large DNA fragments (7.4 and 5.8 kb) into the genomes of mouse embryonic stem cells and zygotes, respectively, using the CRISPR/HDR technique without NHEJ inhibitors was performed and indicate that CRISPR/HDR without NHEJ inhibitors can result in highly efficient gene knock-in, equivalent to CRISPR/HDR with NHEJ inhibitors.

Abstract: A one-way oil-way driven expansion and retraction movement tool includes a hydraulic driving structure, two long arm rods and two connecting rods, wherein, the hydraulic driving structure has a cylinder barrel, a plunger and a shaft rod, the two arm rods are opposite each other and are opposite the two sides of the shaft rod in the lateral direction. One end of each arm rod is respectively pivoted on the cylinder barrel, and the other end of each arm rod is respectively used for arranging a working piece, so that each working piece can move toward or away from each other to execute corresponding work. The connecting rods are respectively pivoted with each of the arm rods and with the connecting part, so that, through the connecting rods, the shaft rod can pull the arm rods to move away from each other or toward each other.

Abstract: A self-adaptive air bag blocking device is disclosed. The device includes an air bag, a first gas conveying pipe and a second gas conveying pipe, wherein an air chamber is formed in the air bag, a ring-shaped abutting surface is formed on the peripheral side of the air bag, so that the abutting surface abuts against a mouth of a container. The first gas conveying pipe is communicated with the air chamber and the outer part of the air bag, so that air enters the air chamber to enable the air bag to expand. The abutting surface is elastically deformed and tightly abuts against the inner wall of the container to form air-tight abutment, and the second gas conveying pipe is arranged on the air bag, so that air enters the container through the second gas conveying pipe, and thus the working liquid is discharged.

Abstract: A tool holder including a main body, a pressing plate and a retaining plate, wherein a long gap is formed between the pressing plate and the main body, so that a cutter can be inserted into the gap. The pawl is adjacent to the pressing plate, and two ends of the shaft rod are respectively connected with the main body, so that the pawl can rotate back and forth relative to the main body according to the shaft rod, thus preventing the pressing plate from pressing and positioning the cutter. According to the invention, the cutter setting and unloading convenience can be improved, and the positioning of the cutter setting can be changed according to the need of operation in damaging the sealant.

Abstract: The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3? end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Abstract: The present invention identifies biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury.

Abstract: The present application discloses a combustion device and a cooking apparatus. The combustion device includes a combustion assembly, the combustion assembly includes a first combustion assembly, and the first combustion assembly includes a box, a first combustor, a top plate and a fire splitting assembly. The box is provided with an opening and a set of intake holes; the first combustor is arranged in the box; the top plate is matched with the box and closes the opening, and is provided with a set of exhaust holes; and the fire splitting assembly is arranged in the box; an intake passage is formed between the fire splitting assembly and an inner bottom surface of the box, one end of the intake passage communicates with the set of intake holes.

Abstract: A method, device, and kit are provided for temporal diagnostics and clinical treatment of neuro injury, neuro disease, or neuro repair, particularly including clinical treatment with precision medicines for the same therapeutic targets as a subset of the temporal biomarkers. Through the measurement of biomarkers in a biological sample from a subject, with at least one biomarker from each of the early, intermediate, and late phases of suspected injury, disease, or repair from a subject, a determination of a subject's injury, disease, or repair is provided with greater sensitivity and/or specificity than previously attainable. As many clinical inventions such an anti-inflammatories and clot disruptors are effective only during certain phases injury, disease, or repair, this knowledge can be used to clinical effect in mitigating secondary injuries and/or diseases.

Abstract: Traumatic brain injury (TBI) is a leading cause of mortality and morbidity around the world. Active immunization with GFAP protein or GFAP peptide or passive immunization with anti-GFAP antibodies or treatment with a GFAP-binding aptamer can be used to reduce the post-TBI induced expression of GFAP, Tau and p-Tau in brain cortex tissues to attenuate the increased serum levels of GFAP after brain injury, and reduce the serum levels of pNF-H, Tau and p-Tau TBI. In addition, GFAP immunization can alleviate anxiety behavior and improve cognitive performance post-injury.

Abstract: The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3? end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Abstract: A lubricating can has a grease cup, bolt, piston, pressing base, bearing placement portion and a rotation driving component. The fixed end of the bolt is fixed on the grease cup bottom. The piston is housed inside the lubricating trough. The piston has a pressing surface, a bearing surface and a lateral ring surface that is tightly fitted on and can slide along the inner wall. The center of the piston is formed with a through hole and a plurality of lubricating guide holes. The through hole is sheathed on the bolt. The lower end pressing portion of the pressing base has a screw hole to fit the bolt. The bearing placement portion is defined and formed by the space between the lower end pressing portion and the bearing surface of the piston. The rotation driving component is configured on the upper end bearing portion of the pressing base.

Abstract: The present invention identifies biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury.

Abstract: The disclosure discloses a gas valve and an oven, where the gas valve includes an intake valve assembly and at least two outlet valve assemblies, the intake valve assembly includes an intake valve body and an intake valve core, the intake valve body is internally provided with a gas inlet, the intake valve core is configured to control the gas inlet to be opened or closed, each outlet valve assembly includes an outlet valve body and an outlet valve core, each outlet valve body is internally provided with a gas outlet, the gas outlet is respectively communicated with the gas inlet, and the outlet valve core is respectively configured to control the corresponding gas outlet to be opened or closed.

Abstract: The invention relies on detection of specific identified proteins, protein breakdown products, and peptide fragments, to diagnose and evaluate traumatic brain injury, spinal cord injury, and any traumatic injury to the CNS in a subject. These analytes (proteins, protein breakdown products thereof, and peptide fragments thereof) are released from injured tissue into blood and/or cerebrospinal fluid, and can be used to identify the central nervous system cell types (i.e. neuron, astrocyte, oligodendrocyte, and the like) or subcellular structure (e.g., axon, dendrites, presynaptic terminal, post-synaptic terminal, and extracellular matrix) affected, and to determine the diagnosis, location, and severity of the injury. Time course measurements of these analytes measured at different times after an injury or suspected injury also are used as tools for diagnosis and prognosis of central nervous system injury.