Abstract: An apparatus for selecting and detecting at least two spectral regions of a light beam includes a selection device, a detection device, and a light suppression device. The light suppression device suppresses reflected and/or scattered light of an illuminating light of a microscope. The light suppression device is located in an optical path between the selection device and the detection device, and includes a graded filter.

Abstract: A scanning microscope for manipulating a sample, the microscope, having a first light source, a second light source, a beam deflector, and an optical device. The first light source is configured to emit an illuminating light beam that follows an illuminating beam. A second light source is configured to produce a manipulating light beam which has a manipulating beam focus and follows a manipulating beam path. The beam deflection device is configured to guide the illuminating light beam and the manipulating beam focus over or through the sample. The optical device is disposed downstream of the second light in the manipulating beam and is configured to modify the size of the manipulating beam focus.

Abstract: The invention relates to a scanning microscope comprising a beam deflecting device (11), which directs an illuminating light beam (5) over or through a sample (21), and comprising a detector (33) for receiving detection light (23) exiting the sample. The scanning microscope comprises an extracting port (67) or another detector (37) and comprises a redirecting device (27), which is synchronized with the beam deflecting device and which directs the detection light according to the deflecting position of the beam deflecting device either to the detector or to the extracting port or to the other detector.

Abstract: A scanning microscope includes a light source for evanescently illuminating a sample disposed on a slide. A point detector receives detection light emanating from a scanning point of the sample. A beam deflection device disposed in an optical path of the detection light can shift a position of the scanning point.

Abstract: A scanning microscope for imaging an object includes a light source and a spectrally selective detection device. An illumination beam path extends from the light source to the object. A detection beam path extends from the object to the detection device. A spectrally selective element useable to select light from the light source is provided. The spectrally selective element is useable to mask out of the detection beam path the selected light from the light source reflected or scattered on the object. An illumination slit diaphragm is disposed in the illumination beam path and configured to generate a linear illumination pattern in a region of the object. A detection slit diaphragm is disposed in the detection beam path and configured to detect the light coming from the linear illumination region from a focal plane so as to provide a confocal slit scanner.

Abstract: A scanning microscope, having a detector, arranged in a detection beam path, for receiving detection light proceeding from a sample, has between the sample and the detector an optical shutter means with which the detection beam path can be blocked. A control means for controlling the shutter means is provided. The detection light beam path can be blocked automatically outside the scanning operation and in the event of an excessive detection light power level.

Abstract: A microscope with a first and a second illumination light beam for illuminating a sample, wherein the first and/or the second illumination light beam evanescently illuminates the sample. For the purpose of CARS testing, the first illumination light beam can be a pump light beam, and the second illumination light beam can be a Stokes light beam. To increase resolution, the first illumination light beam can be an excitation light beam, and the second illumination light beam can be a stimulation light beam.

Abstract: A microscope system for conducting FCS measurements. The system includes an illuminating light source configured to emit an illuminating light at an illuminating wavelength. A target light source is provided and configured to emit a target light for marking an FCS volume in a sample volume at a target wavelength. The target wavelength differs from the illuminating wavelength. The system further includes a plurality of optical elements configured to direct the illuminating light and the target light onto the sample volume.

Abstract: Disclosed is an optical array (2) for the spectrally selective identification of light of a light beam, particularly for identifying light of a detecting light beam (3) in a preferably confocal scanning microscope (1). Said optical array comprises a means (18) for the spatial spectral decomposition of the light beam, means for selecting a given continuous spectral range, and a detector (28). The inventive optical array (2) is characterized by at least one blocking element (25, 26, 27) which can be introduced into the light beam in order to stop down a given partial spectral region located within the selected continuous spectral range.

Abstract: A method for recognizing dark states during the spectroscopic or microscopic examination of fluorescent specimens includes varying an intensity distribution of excitation light by varying an excitation/illumination volume over a plurality of mutually independent measurements. A determination is made as to whether observed time constants change between the measurements. The existence of a dark state is inferred where the observed time constants are unchanged between the measurements.

Abstract: The invention relates to a lens (1) for total internal reflection microscopy. The lens (1) is characterized in that a light source (5, 7) and/or at least one deviating means (31, 3) which deviates the light from a light source (5, 7) into the lens and/or the emitting end (35) of an illuminating optical fiber (37) is arranged in the region of the rear focal plane (3) of the lens (1).

Abstract: A microscope with evanescent sample illumination and a method for testing samples are disclosed. A first evanescent field, which exhibits a first penetration depth in the sample, and a second evanescent field, which exhibits a second penetration depth in the sample that is greater than the first penetration depth, are produced. A detector is provided that detects the first detection light, which exits from the part of the sample illuminated with the first evanescent field, and which produces first detection light data therefrom, and the second detection light, which exits from the part of the sample illuminated with the second evanescent field, and which produces second detection light data therefrom. Furthermore, a processing module is provided for processing the first and second detection light data.

Abstract: Disclosed is a method for analyzing biological samples by means of a scanning microscope. According to said method, at least one screen dot is repeatedly and successively illuminated with a manipulating light beam and an exciting light beam. The interval between the time of illumination with the manipulating light beam and the time of illumination with the exciting light beam is modified, and the fluorescent light yield is measured in accordance with said interval.

Abstract: A method for spatially high-resolution investigation of a structure, marked with a fluorescing substance, of a specimen, the substance being capable of being repeatedly converted from a first state into a second state, the first and second states differing from one another in terms of at least one optical property, encompasses the following steps. Firstly, bring the substance, in a specimen region to be sensed, into the first state; and inducing the second state by way of an optical signal, spatially delimited subregions within the specimen region to be sensed being blanked out in controlled fashion. A protein—target protein—in living cells is used as a structure that is marked with the fluorescing substance, by the fact that a ligand complex encompassing the fluorescing substance is bound to an enzyme via an enzymatic reaction in the cell, the enzyme being expressed as a fusion protein together with the target protein to be investigated.

Abstract: Disclosed is a method for analyzing biological samples by means of a scanning microscope. According to said method, at least one screen dot is repeatedly and successively illuminated with a manipulating light beam and an exciting light beam. The interval between the time of illumination with the manipulating light beam and the time of illumination with the exciting light beam is modified, and the fluorescent light yield is measured in accordance with said interval.

Abstract: A scanning microscope having a light source that emits an illuminating light beam, for illumination of a sample. The illuminating light beam extends along an illumination beam path and can be guided over and/or through the sample using a beam deflection device. A mirror which can be introduced in guided fashion into the illumination beam path directs a manipulating light beam via the beam deflection device onto the sample.

Abstract: The invention relates to a scanning microscope comprising a beam deflecting device (11), which directs an illuminating light beam (5) over or through a sample (21), and comprising a detector (33) for receiving detection light (23) exiting the sample. The scanning microscope comprises an extracting port (67) or another detector (37) and comprises a redirecting device (27), which is synchronized with the beam deflecting device and which directs the detection light according to the deflecting position of the beam deflecting device either to the detector or to the extracting port or to the other detector.

Abstract: A microscope with a first and a second illumination light beam for illuminating a sample, wherein the first and/or the second illumination light beam evanescently illuminates the sample. For the purpose of CARS testing, the first illumination light beam can be a pump light beam, and the second illumination light beam can be a Stokes light beam. To increase resolution, the first illumination light beam can be an excitation light beam, and the second illumination light beam can be a stimulation light beam.

Abstract: A scanning microscope includes at least one light source, an acousto-optical element, a beam deflection device and a beam guiding device. The at least one light source generates an illuminating light beam. The acousto-optical element spatially splits a sub-light beam from the illuminating light beam and adjusts an optical power of the illuminating light beam. The beam deflection device scans the illuminating light beam over or through a sample. The beam guiding device directs the sub-light beam onto the sample.

Abstract: A microscope objective includes an optical fiber. The optical fiber can deliver light for total internal reflection microscopy. The optical fiber can couple illumination light directly into the microscope objective through the optical fiber.