Abstract: A time-ordered series of measurements of a polymer made during translocation of the polymer through a Nanopore are analysed. The measurements are dependent on the identity of k-mers in the Nanopore, a k-mer bring k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.

Abstract: An easy-to-use paint roller cleaning device with multiple cleaning members sized for interfacing with rollers and naps of various sizes while the user holds onto the device along a handle. The cleaning members have an opening encircled by a roller interface wall which squeezes tightly over the nap while its paint-scraping rim removes the paint or other liquid from the roller nap and the paint-guiding wall urges the liquid downward to exit the nap. The paint roll cleaner tool will scrape the entire length of the nap before clearing the tip of the nap farthest from the painting handle. The cleaning structures come in sizes suited for standard and mini rollers with a variety of naps including ¼ inch, ? inch, and ½ inch.

Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.

Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Abstract: A method for non-custodial backup of a secret (1021) by the owner of said secret, assisted by a multiplicity n of recovery agents, each having individual public keys and private keys, and the owner having a first value (1013), comprising providing the owner with a first computer program, which at rest will reside on a computer readable medium, configured to enroll the secret by computing a public data set (1023), as a function of the owner's first value, each of the n recovery agents' public keys (1011), and a nonce (1015). A method for recovery of the secret (1021) is also disclosed.

Abstract: A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.

Abstract: A verification probe system is configured to verify an automated test platform and includes: an integrated circuit test probe assembly; and a moveable platform configured to position the integrated circuit test probe assembly proximate one of more conductive pins included within a test socket assembly of the automated test platform.

Abstract: A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.

Abstract: The invention resides in a method of determining a sequence of a target polymer, or part thereof, comprising polymer units comprising canonical and non-canonical polymer units. The method comprises taking a series of measurements of a signal relating to the target polymer wherein a measurement of the signal is dependent upon a plurality of polymer units, and wherein the polymer units of the target polymer modulate the signal, and wherein a non-canonical polymer unit modulates the signal differently from a corresponding canonical polymer unit. The series of measurements are analysed using a machine learning technique that attributes a measurement of a non-canonical polymer unit to being a measurement of a respective corresponding canonical polymer unit. The sequence of the target polymer, or part thereof, is determined from the analysed series of measurements. A non-canonical polymer unit identified from the analysis can be additionally or alternatively determined.

Abstract: A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide.

Abstract: Provided herein, in some embodiments, are methods of determining whether a target nucleic acid comprises a particular barcode sequence.

Abstract: A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide.

Abstract: A method of eliminating microbes in an overflow space in a sink having a sink basin may include obtaining a liquid dispensing and effluent shielding container assembly including a splash guard including a splash guard shield and an injection nozzle carried by the splash guard shield, the injection nozzle having an injection nozzle bore and at least one injection opening in the injection nozzle, the at least one injection opening, disposed in fluid communication with the injection nozzle bore; inserting the injection nozzle of the liquid dispensing and effluent shielding container assembly in the at least one overflow opening in the sink basin until the splash guard substantially covers the at least one overflow opening; and expelling the antimicrobial or cleaning agent liquid through the injection nozzle and the at least one injection opening and into the overflow space in the sink basin of the sink, respectively.

Abstract: A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.

Abstract: A liquid dispensing and effluent shielding container assembly includes a flexible container having a container interior and a splash guard including a splash guard shield carried by the container. The splash guard shield may have a shield opening disposed in fluid communication with the container interior of the container. An injection nozzle is carried by the splash guard shield. The injection nozzle has a injection nozzle bore disposed in fluid communication with the shield opening of the splash guard shield and at least one injection opening in the injection nozzle, the at least one injection opening disposed in fluid communication with the injection nozzle bore. A method of eliminating microbes in an overflow space in a sink is also disclosed.

Abstract: A method of eliminating microbes in an overflow space in a sink having a sink basin and at least one overflow opening in the sink basin and communicating with the overflow space may include obtaining a liquid dispensing and effluent shielding container assembly including a flexible container having a container interior and a splash guard including a splash guard shield carried by the container and an injection nozzle carried by the splash guard shield, the injection nozzle having a injection nozzle bore disposed in fluid communication with the container interior of the container and at least one injection opening in the injection nozzle, the at least one injection opening disposed in fluid communication with the injection nozzle bore; placing an antimicrobial or cleaning agent liquid in the container interior of the container; inserting the injection nozzle of the liquid dispensing and effluent shielding container assembly in the at least one overflow opening in the sink basin until the splash guard substanti

Abstract: Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.

Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Abstract: A method by which a hardware security module can attest remotely to its measure of trust as determined by its security certifications and the Level of Assurance it can be relied on to support without the human witnessing elements that are currently used to validate this trust. In a further embodiment the Level of Assurance can be transported to a second hardware security module.

Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.