Abstract: The invention provides materials and methods for the detection of nucleic acid expression via the 3? portion of expressed sequences. Embodiments of the invention include the use of microarrays comprising nucleic acid probes that are complementary to the 3? end of expressed sequences and by the use of quantitative PCR (Q-PCR) based amplification of sequences found at or near the 3? end of expressed sequences. The invention may be used to detect the presence of expressed nucleic acids encoding particular gene products (sequences present in a “transcriptome”).

Abstract: The present invention provides methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

Abstract: This invention relates to the immune response activity of eosinophils, and especially activated eosinophils, and provides isolated nucleic acids, polypeptides, including fragments, subunits and variants of the nucleic acids and polypeptides, as well as related compositions, probes, vectors, host cells, antibodies, and methods. In one aspect, the invention provides isolated nucleic acids encoding specific eosinophil-derived polypeptides or fragments or variants. Generally, the nucleic acids comprise a sequence encoding at least one domain or region associated with an eosinophil biochemical pathway function or associated with activated eosinophils. And, generally, corresponding domains or regions of polypeptides of the invention have at least a five amino acid fragment encoded by one of SEQ NOS: 1-71.

Abstract: Methods and compositions are provided for the detection of breast cancer based upon the identification of three biomarkers for non-normal breast cells. The biomarkers were identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer. Two biomarkers display increased expression in non-normal cells while the third biomarker displays decreased expression in non-normal cells.

Abstract: Methods and compositions for the identification of breast cancer grade signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or more particular stages and/or grades of breast cancer.

Abstract: The present invention relates to the use of molecular histological signatures to interpret and correlate cytological specimens with the presence or absence of disease and the progression thereof. The invention provides molecular signatures for use in the study and/or diagnosis of diseased cells and tissues of a cytological specimen relative to a solid (e.g. histological) sample.

Abstract: This invention relates to nucleic acids derived from osteoarthritis (OA) tissue as well as compounds and compositions made using these nucleic acids. A variety of nucleic acid and polypeptide compounds and compositions are described and specifically disclosed. The nucleic acids or polypeptides may be contained within vectors or host cells and then used to produce agents such as nucleic acids, polypeptides, fragments of polypeptides, antibodies, and variants of each. These molecules can be used to diagnose or treat osteoarthritis, or to analyze the disease-modifying activity of OA drugs. Cells containing one or more nucleic acids or polypeptides of the invention can also be used as targets in high-throughput screening methods, particularly in screening for compounds designed to identify compositions affecting osteoarthritis.