Abstract: The present disclosure provides systems, kits and methods for determining the copy number of a mouse TCR transgene. The primers are specific to the conserved regions of the mouse TCR gene. The primers/probes provide good amplification efficiency and can quickly and accurately determine the copy number of a mouse TCR transgene.

Abstract: Anti-BCMA antibodies and chimeric antigen receptors (CARs) are provided. Immune cells expressing the anti-BCMA CAR can be used to treat cancer. The anti-BCMA antibodies and CARs can recognize the extracellular domains of human BCMA. The anti-BCMA CAR T cells show specific cytotoxicity towards BCMA-positive target cells.

Abstract: The invention relates to a method for guaranteeing the reliability of real-time scrambling and message transmission by a train control center system. The method comprises the following steps: S1, starting the system for offline message loading and reading; S2, obtaining message data to be scrambled; S3, sending the message to be scrambled to a scrambling operation board for scrambling; S4, performing inverse decoding processing on an operation board; S5, performing comparison after decoding; and S6, outputting effective message data. Compared with the prior art, the invention has the advantages of being capable of guaranteeing the safety and reliability of message data.

Abstract: Provided are a CD22-targeted chimeric antigen receptor, a preparation method therefor and an application thereof. The chimeric antigen receptor comprises a leader sequence, a CD22-targeted scFv, a hinge region, a transmembrane region, and an intracellular signal domain. Provided are a nucleic acid molecule encoding the chimeric antigen receptor and a corresponding expression vector, a CAR-T cell, and an application thereof. The chimeric antigen receptor targets CD22 positive cells and can be used for treating CD22-positive B-cell leukemia, and some CD19-negative and CD22-positive patients in which acute B-cell leukemia has recurred after anti-CD19 CAR-T treatment.

Abstract: A method, apparatus, computer device, and storage medium for automatic design of analog circuits based on tree structure. The method includes: setting the maximum height and growth direction of the tree structure; randomly calling the node from the function node library as the parent node; randomly calling the node from the function node library and the port node library as the child according to the growth direction node; if the child node is a terminal node, generating a tree structure; checking the tree structure, if the tree structure satisfies the preset conditions, obtaining the circuit topology and device parameter that conform to the circuit rules; evolving the circuit topology and device parameter to generate an analog circuit. The embodiments achieve the effect of making the tree structure of the designed analog circuit more reasonable.

Abstract: The present invention provides a combined chimeric antigen receptor targeting CD19 and CD20 and application thereof. Specifically, the present invention provides a combined chimeric antigen receptor targeting CD19 and CD20, which comprises a scFv targeting CD19 and CD20, a hinge region, a transmembrane region, and an intracellular signaling domain. The present invention provides a nucleic acid molecule encoding the chimeric antigen receptor and a corresponding expression vector, a CAR-T cell, and applications thereof. The experimental results show that the chimeric antigen receptor provided by the present invention shows extremely high killing ability against tumor cells. The chimeric antigen receptor of the present invention targets CD19 and/or CD20 positive cells and can be used to treat CD19 and/or CD20 positive B-cell lymphoma, leukemia and other diseases.

Abstract: A method of logistics management based on QR code, the method includes: acquiring a video stream of a logistics box taken by a camera of a logistics box passing through an identification area, the logistics box having a corresponding QR code affixed to its surface; performing target detection on the video stream based on a predetermined neural network model to obtain a QR code image and a logistics box image, respectively; identifying the QR code image to generate QR code information; detecting and tracking the logistics box images in sequence to generate logistics box information; matching the QR code information and logistics box information, generating matching information, and updating logistics inventory information based on the matching information.

Abstract: Provided is a combined chimeric antigen receptor targeting CD19 and CD20 and application thereof. Specifically, provided is a combined chimeric antigen receptor targeting CD19 and CD20, which comprises a scFv targeting CD19 and CD20, a hinge region, a transmembrane region, and an intracellular signaling domain. Provided is a nucleic acid molecule encoding the chimeric antigen receptor and a corresponding expression vector, a CAR-T cell, and applications thereof. The experimental results show that the chimeric antigen receptor shows extremely high killing ability against tumor cells. The chimeric antigen receptor targets CD19 and/or CD20 positive cells and can be used to treat CD19 and/or CD20 positive B-cell lymphoma, leukemia and other diseases.

Abstract: Provided are a chimeric antigen receptor targeting CD20 antigen and a preparation method thereof. The extracellular antigen binding domain of the chimeric antigen receptor includes an antibody heavy chain variable region shown in SEQ ID NO: 7 or 9 or 33 and an antibody light chain variable region shown in SEQ ID NO: 11 or 13 or 35, and is capable of killing tumor cells.

Abstract: The invention provides an isolated or purified T-cell receptor (TCR) having antigenic specificity for MHC Class II-restricted MAGE-A3. The invention further provides related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, and populations of cells. Further provided by the invention are antibodies, or an antigen binding portion thereof, and pharmaceutical compositions relating to the TCRs of the invention. Methods of detecting the presence of cancer in a host and methods of treating or preventing cancer in a mammal are further provided by the invention.

Abstract: Provided are a chimeric antigen receptor targeting CD20 antigen and a preparation method thereof. The extracellular antigen binding domain of the chimeric antigen receptor includes an antibody heavy chain variable region shown in SEQ ID NO: 7 or 9 or 33 and an antibody light chain variable region shown in SEQ ID NO: 11 or 13 or 35, and is capable of killing tumor cells.

Abstract: The present invention provides a BCMA-targeted chimeric antigen receptor (CAR) as well as a preparation method therefor and an application thereof. Specifically, the present invention provides the BCMA-targeted CAR, which comprises a BCMA-targeted scFv, a hinge region, a transmembrane region, and an intracellular signal structure domain. The present invention provides a nucleic acid molecule for coding the CAR and a corresponding expression vector as well as CAR-T cells and application thereof. The CAR of the present invention targets BCMA-positive cells, and can be used for treating BCMA-positive B-cell lymphoma, multiple myeloma and plasma cell leukemia.

Abstract: The invention provides compositions, devices, methods and kits allowing for rapid diagnosis of infectious diseases via extraction-free, direct PCR techniques using combined biological samples.

Abstract: There are described headphones comprising earcups to be placed about ears of a user, with a headband linking the earcups and to be extending above a head of the user. A flexible band distinct from the headband is secured below the headband for contact with the head of the user. Removable headband sensors are embedded in the flexible band and have a portion thereof protruding downwardly from the flexible band to reach the scalp. The flexible band has a flexibility which makes the flexible band deform under the weight of the earcups to conform with the head of the user to ensure high quality contact between the headband electrodes and the scalp. There are further provided earcup electrodes on the earcups for contact with a region on or behind an ear of the user. Signals from the electrodes can be used for different purposes such as concentration monitoring and feedback.

Abstract: A method, apparatus, computer device, and storage medium for automatic design of analog circuits based on tree structure. The method includes: setting the maximum height and growth direction of the tree structure; randomly calling the node from the function node library as the parent node; randomly calling the node from the function node library and the port node library as the child according to the growth direction node; if the child node is a terminal node, generating a tree structure; checking the tree structure, if the tree structure satisfies the preset conditions, obtaining the circuit topology and device parameter that conform to the circuit rules; evolving the circuit topology and device parameter to generate an analog circuit. The embodiments achieve the effect of making the tree structure of the designed analog circuit more reasonable.

Abstract: Provided is a non-naturally occurring T population, of which the proportion of T memory stem cells satisfies C1?50%. The T cell population comprises a tumor antigen-targeting universal CAR-T cell. Further provided is a method for preparing the CAR-T cell. The CAR-T cell is higher in amplification rate and better in phenotype, IFN-gamma releasing capacity and target cell killing capability.

Abstract: An optimization method, apparatus, computer device and storage medium for engine model. The method includes: setting an initial population according to a predetermined number of solutions; obtaining a first output set of the initial population on the engine model; determining a first fitness value; operating selection, crossover, and mutation on the initial population according to the first fitness value to obtain an evolved population, and the initial population and the evolved population constitute a to-be-selected population; obtaining a second output set of the evolved population on the engine model; determining a second fitness value; selecting engine model parameters in the to-be-selected population to form a new population according to the second fitness value; and optimizing the engine model by using the engine model parameters in the new population if a termination condition is met.

Abstract: A method of logistics management based on QR code, the method includes: acquiring a video stream of a logistics box taken by a camera of a logistics box passing through an identification area, the logistics box having a corresponding QR code affixed to its surface; performing target detection on the video stream based on a predetermined neural network model to obtain a QR code image and a logistics box image, respectively; identifying the QR code image to generate QR code information; detecting and tracking the logistics box images in sequence to generate logistics box information; matching the QR code information and logistics box information, generating matching information, and updating logistics inventory information based on the matching information.

Abstract: The present invention provides a BCMA-targeted chimeric antigen receptor (CAR) as well as a preparation method therefor and an application thereof. Specifically, the present invention provides the BCMA-targeted CAR, which comprises a BCMA-targeted scFv, a hinge region, a transmembrane region, and an intracellular signal structure domain. The present invention provides a nucleic acid molecule for coding the CAR and a corresponding expression vector as well as CAR-T cells and application thereof. The CAR of the present invention targets BCMA-positive cells, and can be used for treating BCMA-positive B-cell lymphoma, multiple myeloma and plasma cell leukemia.

Abstract: Provided are a chimeric antigen receptor targeting CD20 antigen and a preparation method thereof. The extracellular antigen binding domain of the chimeric antigen receptor includes an antibody heavy chain variable region shown in SEQ ID NO: 7 or 9 or 33 and an antibody light chain variable region shown in SEQ ID NO: 11 or 13 or 35, and is capable of killing tumor cells.