Abstract: Methods for the delivery of genes to improve cardiac function including the use of viral vectors, isolation of the heart from systemic circulation, and induction of hypothermia/cardiac arrest are described. The method results in high-level, long-term expression of reporter genes and enhanced cardiac function in hamster models of heart disease.

Abstract: Provided herein is a retroviral vector comprising, and capable of expressing, a nucleotide of interest (NOI), wherein the NOI encodes an RNA or protein which is harmful to a cell.

Abstract: The lip-type seal of the present invention is a lip-type seal with which the outer periphery of a rotational shaft (S) supported by a predetermined housing (H) is sealed. The lip-type seal is made up of a first annular reinforcing member (11) and a first sealing member (12). The first reinforcing member (11) includes a wall surface part (11a) defining a hole through which the rotational shaft (S) is passed and a cylindrical part (11b) bent from the outer edge of the wall surface part (11a). The first sealing member (12) includes an annular base (12a) that is joined to the housing (H), a first lip part (12b) that extends almost conical from the base (12a) inwardly in the radial direction and that comes into contact with the rotational shaft (S), and an annular concave part (11c) formed on the base (12a) so as to detachably fit the cylindrical part (11b). Accordingly, a desired sealing capability can be secured, and the components can be easily assembled, disassembled, and recycled.

Abstract: The present invention provides a prophylactic and/or therapeutic agent for pulmonary hypertension, comprising an antagonistic mutein of MCP-1 or a salt thereof, a DNA molecule comprising a nucleotide sequence encoding the antagonistic mutein of MCP-1, or a neutralizing antibody against MCP-1. The antagonistic mutein of MCP-1 or a salt thereof, the DNA molecule having a nucleotide sequence encoding the antagonistic mutein of MCP-1, or the neutralizing antibody against MCP-1 has hypotensive activity, and thus is useful as a pharmaceutical agent for preventing and/or treating pulmonary hypertension (primary pulmonary hypertension, in particular).

Abstract: The lip-type seal of the present invention is a lip-type seal with which the outer periphery of a rotational shaft (S) supported by a predetermined housing (H) is sealed. The lip-type seal is made up of a first annular reinforcing member (11) and a first sealing member (12). The first reinforcing member (11) includes a wall surface part (11a) defining a hole through which the rotational shaft (S) is passed and a cylindrical part (11b) bent from the outer edge of the wall surface part (11a). The first sealing member (12) includes an annular base (12a) that is joined to the housing (H), a first lip part (12b) that extends almost conical from the base (12a) inwardly in the radial direction and that comes into contact with the rotational shaft (S), and an annular concave part (11c) formed on the base (12a) so as to detachably fit the cylindrical part (11b). Accordingly, a desired sealing capability can be secured, and the components can be easily assembled, disassembled, and recycled.

Abstract: Provided herein is a retroviral vector comprising, and capable of expressing, a nucleotide of interest (NOI), wherein the NOI encodes an RNA or protein which is harmful to a cell.

Abstract: The invention relates to materials and methods for improved vaccination strategies and in particular to the use of lentivirus comprising nucleic acid encoding an antigen, or antigen presenting cells transduced with such lentivirus, to stimulate immune responses against the encoded antigen, in heterologous prime-boost vaccination regimes.

Abstract: The face pressure of a seal lip for sealing is made sharp to improve the sealing ability and the seal lip is prevented from being deformed abnormally when a shaft is inserted therein. In the constitution, the inside diameter of the corner face of the sealing face is smaller than the outside diameter of the shaft. When the outside surface of the support section of the support ring expands the expandable inner surface, the inside diameter of the sealing face is also expanded diametrically to closely contact the corner face of the sealing face with the shaft.

Abstract: The present invention provides a prophylactic and/or therapeutic agent for pulmonary hypertension, comprising an antagonistic mutein of MCP-1 or a salt thereof, a DNA molecule comprising a nucleotide sequence encoding the antagonistic mutein of MCP-1, or a neutralizing antibody against MCP-1.

Abstract: In a lip type seal, a case is tightly fitted and fixed to an inner circumferential surface of a housing through a gasket. A resin lip member constituted by an inner circumferential part extending in a bent state from a diameter direction part and its inner circumference to a sealed space side and forming a seal surface between an outer circumferential part of a rotary shaft and the resin lip member; a rear surface support plate disposed to abut on a rear surface of the diameter direction part of the resin lip member; and a stopper with a rear surface supported to a support surface of the housing through a second resin lip member and a second rear surface support plate and the top end coming close to or coming into contact with a rear surface of the inner circumferential part of the rear surface support plate, are provided on the inner circumference of the housing.

Abstract: The face pressure of a seal lip for sealing is made sharp to improve the sealing ability and the seal lip is prevented from being deformed abnormally when a shaft is inserted therein. In the constitution, the inside diameter of the corner face of the sealing face is smaller than the outside diameter of the shaft. When the outside surface of the support section of the support ring expands the expandable inner surface, the inside diameter of the sealing face is also expanded diametrically to closely contact the corner face of the sealing face with the shaft.

Abstract: An elastomer lip member with its rear supported by a pressure resisting back-up ring, and a synthetic resin lip member arranged on a rear side of the elastomer lip are comprised, and on the inner circumferential surface of the seal lip portion of the elastomer lip member, helical grooves generating a pumping action of a fluid introducing direction from an inner space of machine side when a shaft rotates are formed. When a pressure in the inner space of machine is MPa (gage), by making a contact width between the seal lip portion of the elastomer lip member and a shaft at least 0.6 mm, so that a surface pressure maximum portion of the seal lip portion of the elastomer lip member to the shaft is biased or maldistributed toward its rear side whereby a fluid introduction by said helical grooves is performed satisfactorily.

Abstract: A lip type seal is provided with an outer ring part (31) which is tightly fitted to a seal installation part (12) of a housing (1); a seal main body (32) which is fixed to the outer ring part (31) and whose inner circumferential part extending in a bent state to the side of a sealed space (A) is sealably brought into slidable contact with an outer circumferential surface of a rotary shaft (2) inserted in the housing (1); a rear surface support plate (33) which is arranged on the rear side of the seal main body and whose outer circumferential part together with the seal main body (32) is fixed to the outer ring part (31); and a movable support plate (34) which is engaged movably in a radial direction between the seal main body (32) and the rear surface support plate (33) and whose inner diameter is clearance fitted to the outer circumferential surface of the rotary shaft (2).

Abstract: Methods for the delivery of genes to improve cardiac function including the use of viral vectors, isolation of the heart from systemic circulation, and induction of hypothermia/cardiac arrest are described. The method results in high-level, long-term expression of reporter genes and enhanced cardiac function in hamster models of heart disease.

Abstract: An elastomer lip member 12 that its rear is supported with a pressure resisting back-up ring 13, and a synthetic resin lip member 14 arranged on a rear side of the elastomer lip 12 are comprised, and on the inner circumferential surface of the seal lip portion 124 of the elastomer lip member 12, helical grooves 125 generating a pumping action of a fluid introducing direction from an inner space S1 of machine side when a shaft 2 rotates are formed. When a pressure in the inner space of machine S1 is 1 MPa [gage], by making a contact width between the seal lip portion 124 of the elastomer lip member 12 and a shaft 2 at least 0.6 mm, so that a surface pressure maximum portion of the seal lip portion 124 of the elastomer lip member 12 to the shaft 2 is biased or maldistributed toward its rear side whereby a fluid introduction by said helical grooves 125 is performed satisfactorily.

Abstract: A lip type seal is provided with an outer ring part (31) which is tightly fitted to a seal installation part (12) of a housing (1); a seal main body (32) which is fixed to the outer ring part (31) and whose inner circumferential part extending in a bent state to the side of a sealed space (A) is sealably brought into slidable contact with an outer circumferential surface of a rotary shaft (2) inserted in the housing (1); a rear surface support plate (33) which is arranged on the rear side of the seal main body and whose outer circumferential part together with the seal main body (32) is fixed to the outer ring part (31); and a movable support plate (34) which is engaged movably in a radial direction between the seal main body (32) and the rear surface support plate (33) and whose inner diameter is clearance fitted to the outer circumferential surface of the rotary shaft (2).

Abstract: In a lip type seal, a case (31) is tightly fitted and fixed to an inner circumferential surface of a housing (1) through gasket means (32e). A resin lip member (33) constituted by an inner circumferential part (33c) extending in a bent state from a diameter direction part (33a) and its inner circumference to a sealed space (S1) side and forming a seal surface between an outer circumferential part of a rotary shaft (2) and the resin lip member (33); a rear surface support plate (36) disposed to abut on a rear surface of the diameter direction part (33a) of the resin lip member; and a stopper (37b) with a rear surface supported to a support surface (13) of the housing (1) through a second resin lip member (34) and a second rear surface support plate (38) and the top end coming close to or coming into contact with a rear surface of the inner circumferential part of the rear surface support plate 36, are provided on the inner circumference of the housing (1).

Abstract: The present invention relates to a lip-type seal of the outer periphery rubber type having a stationary sealing portion made of rubber on the outer periphery of a reinforcing ring. In view of the fact that there is produced a gap corresponding to the rubber thickness of the stationary sealing portion between the outer periphery of the reinforcing ring and inner periphery of the seal attachment hole, the invention has an object to permit certain supporting of the reinforcing ring by means of a stop functional member by expanding the engagement area engaging with the stop functional member.

Abstract: A sealing apparatus has a metal ring having a cylindrical portion and an inward flange portion bending from the open-air side end of the cylindrical portion radially inward; a first sealing member formed integral with the metal ring, and having a first rubber-like sealing lip, and a flange-shaped interposed portion held between the inward flange position of the metal ring and the radial portion of the first sealing member. A second sealing member made of a resin material, extends from the inside-diameter end of the interposed portion axially toward the sealing liquid side, and is located in a gap between the open-air side surface of the first sealing lip and a rotary member, having the inner periphery thereof in slidable close contact with the surface of the rotary member, and regulates radial deformation of the first sealing lip. A screw pumping portion is provided to transport the liquid between the sliding surfaces to the sealing liquid side on rotation of the rotary member.

Abstract: A molding station for molding a colored product of a synthetic resin mixed with a colorant and a coating station for applying a colored clear coat to the surface of the molded colored product are disposed adjacent to each other for highly efficient production of colored clear coated, molded colored products. The molded colored product is charged directly from the molding station into the coating station. The hue of the molded colored product and the hue of the colored clear coat are seen as mixed to the eye. A plurality of hues may be available for each of the colorant and the colored clear coat, so that a variety of hues can be achieved on colored products by a combination of hues of the colorant and the colored clear coat without maintaining a large stock or inventory of different colorants or colored clear coats.