Abstract: An object of the present invention is to provide a method capable of detecting activity of an enzyme that strictly recognizes a substrate, such as acetylcholinesterase (AChE). There is provided a method for detecting activity of an enzyme in a biological sample using a microdevice, the method including a step of bringing the biological sample into contact with a substrate analog or a natural substrate, and a compound represented by General Formula (I) below or a salt thereof, wherein the substrate analog or the natural substrate generates a compound A having a thiol group (R—SH), a selenol group (R—SeH), or a poly sulfur atom (R—(S)n-H) (R is a hydrogen atom or an alkyl group, and n is 1 to 5) by a reaction with the enzyme, and fluorescence is observed or measured, the fluorescence being emitted or enhanced from a fluorescent product generated by the reaction of the compound represented by General Formula (I) or the salt thereof with the thiol group, the selenol group, or the poly sulfur atom.

Abstract: A compound is represented by the following general formula (I): The compound or a salt thereof provides a fluorescent probe for detecting carboxypeptidase activity In the formula, R1 represents an alkyl group having 1 to 6 carbon atoms or an alkoxy group having 1 to 6 carbon atoms; T represents an amino acid residue or a residue of an amino acid derivative; S represents a C-terminal amino acid residue. represents a fluorophore, and the fluorophore is a fluorophore in which an absorption/fluorescence wavelength greatly changes or a quenched state changes to a fluorescent state by elimination of the —P(?O)(—R1)-T-S moiety.

Abstract: A probe capable of inducing destruction of actin fibers in a spatiotemporally selective manner, and a method using the probe. A compound represented by the following Formula (I) or a salt thereof. R1 may represent, when present, the same or different monovalent substituents present on a benzene ring; R2 may represent a bromine atom or an iodine atom; m may be an integer of 0 to 4; and n may be an integer of 1 or 2.

Abstract: To provide a synthesis scheme capable of easily synthesizing various kinds of fluorescent probes. A method for preparing a compound represented by Formula (III) below, the method including steps (1) to (5).

Abstract: A fluorescent probe can be used to detect and visualize carboxypeptidase activity with high sensitivity. The fluorescent probe has, as a base nucleus, a fluorescent skeleton that functions in the visible light region, and makes carboxypeptidase activity detectable and visible, for example, within a cell or clinical specimen, with high sensitivity.

Abstract: [Problem] To provide a fluorescent probe capable of detecting pancreatic cancer specifically. [Solution] A fluorescent probe for use in the detection of pancreatic cancer, which comprises a compound represented by general formula (I) or a salt thereof.

Abstract: [Problem] To provide a novel compound promising as a probe for a boron neutron capture therapy (BNCT). [Solution] A compound represented by general formula (I) below or a salt thereof.

Abstract: To provide novel compounds that are promising as prodrug-type anticancer agents, a compound represented by general formula (I) or a salt thereof is provided.

Abstract: It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing ?-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP.

Abstract: Provided is a novel fluorescent probe. A compound of the following general formula (I) or a salt thereof.

Abstract: A novel non-fluorescent rhodamine dye forms a twisted intramolecular charge transfer state. A substituent that causes steric hindrance is introduced at an ortho position of a dimethylamino group on the xanthene ring of tetramethylrhodamine, which is a general rhodamine that exhibits strong fluorescence, and a certain amount of twist is imparted in a ground state. As a result, the formation of the twisted intramolecular charge transfer state is promoted in the excited state and non-fluorescence is exhibited.

Abstract: A factor that is caused by a nucleic acid and influences the kinetics of an extracellular vesicle is screened. A library of barcoded extracellular vesicles is provided.

Abstract: Provided is a novel fluorescent probe which can be used in a spray manner, has an outstandingly high sensitivity-specificity with instantaneousness, and enables detection of a brain tumor.

Abstract: A fluorescent probe for sialidase activity detection, is a compound represented by the following general formula or a salt thereof. R1, if present, represents the same or different monovalent substituent present on a benzene ring. R2 and R3 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R4 and R5 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R6 represents a hydrogen atom, an alkyl group having 1 to 5 carbon atoms, or a fluorinated alkyl group having 1 to 5 carbon atoms. R6? represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms, and R6? may form, together with R3 or R5 a five to seven-membered heterocyclyl or heteroaryl containing a nitrogen atom to which R6? is bonded.

Abstract: As a technique for detecting an influenza virus with an improved accuracy, there is provided a method for detecting an influenza virus in a biological sample by using a first probe, which is decomposed by an influenza virus-derived neuraminidase and a bacterium-derived neuraminidase to generate an optically detectable signal, and a second probe, which is decomposed by the bacterium-derived neuraminidase to generate an optically detectable signal and not decomposed by the influenza virus-derived neuraminidase.

Abstract: A fluorescent probe is capable of detecting breast cancer, lung cancer, and squamous cell carcinoma. The fluorescent probe is also capable of detecting a benign tumor of a mammary gland.

Abstract: A compound is represented by the following general formula (I): The compound or a salt thereof provides a fluorescent probe for detecting carboxypeptidase activity. In the formula, R1 represents an alkyl group having 1 to 6 carbon atoms or an alkoxy group having 1 to 6 carbon atoms; T represents an amino acid residue or a residue of an amino acid derivative; S represents a C-terminal amino acid residue. represents a fluorophore, and the fluorophore is a fluorophore in which an absorption/fluorescence wavelength greatly changes or a quenched state changes to a fluorescent state by elimination of the —P(?O)(—R1)-T-S moiety.

Abstract: A novel non-fluorescent rhodamine dye forms a twisted intramolecular charge transfer state. A substituent that causes steric hindrance is introduced at an ortho position of a dimethylamino group on the xanthene ring of tetramethylrhodamine, which is a general rhodamine that exhibits strong fluorescence, and a certain amount of twist is imparted in a ground state. As a result, the formation of the twisted intramolecular charge transfer state is promoted in the excited state and non-fluorescence is exhibited.

Abstract: A fluorescent probe can be used to detect and visualize carboxypeptidase activity with high sensitivity. The fluorescent probe has, as a base nucleus, a fluorescent skeleton that functions in the visible light region, and makes carboxypeptidase activity detectable and visible, for example, within a cell or clinical specimen, with high sensitivity.

Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.