Patents by Inventor Yasuteru Urano
Yasuteru Urano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140248654Abstract: Provided is a compound represented by formula (I), which can be used as a fluorescent probe that becomes highly fluorescent only in an intracellular acidic environment and can be adapted to the fluorescent imaging of an intracellular vesicle that is a microstructure. In the formula, R1 and R2 independently represent a hydrogen atom, or an alkyl group which may be substituted (wherein R1 and/or R2 represents an alkyl group which may be substituted); R3, R4, R5, R6, R7 and R8 independently represent a hydrogen atom, a halogen atom, or the like; X represents a functional group to which a labeling site or a target-accumulating site can be introduced, or the like; Y represents a halogen atom, an alkyl group which may be substituted, or the like; m represents an integer of 0 to 5; and n represents an integer of 0 to 5.Type: ApplicationFiled: September 6, 2012Publication date: September 4, 2014Applicant: THE UNIVERSITY OF TOKYOInventors: Yasuteru Urano, Tetsuo Nagano, Daisuke Asanuma, Kenzo Hirose, Shigeyuki Namiki, Yousuke Takaoka
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Publication number: 20140206992Abstract: Methods for identifying the presence of cancerous cells or tissue by: applying a compound of formula (I), or salts thereof, to a living body, wherein R1 represents hydrogen or the same or different one to four substituents; R2, R3, R4, R5, R6, and R7 independently represent hydrogen, hydroxy, alkyl, or halogen; R8 and R9 independently represent hydrogen or alkyl; and X represents C1-C3 alkylene; and detecting fluorescence emitted by a compound of formula (II), wherein R1, R2, R3, R4, R5, R6, R7, R8, and R9 are as defined above; wherein fluorescence emitted by a compound of formula (II) is indicative of the presence of cancerous cells or tissue.Type: ApplicationFiled: January 24, 2014Publication date: July 24, 2014Applicant: THE UNIVERSITY OF TOKYOInventors: Yasuteru URANO, Tetsuo NAGANO, Masayo SAKABE
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Patent number: 8697383Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.Type: GrantFiled: May 25, 2011Date of Patent: April 15, 2014Assignees: Mitsubishi Rayon Co., Ltd., The University of TokyoInventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
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Patent number: 8673957Abstract: A compound represented by the formula (I) wherein R1 and R2 represent amino groups that substitute at adjacent positions on the benzene ring, wherein one of the amino groups may have one alkyl group; R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group, R5 and R8 independently represent a C1-6 alkyl group, R6 and R9 represent a carboxy-substituted C1-6 alkyl group, an alkoxycarbonyl-substituted C1-6 alkyl group, a sulfo-substituted C1-6 alkyl group, or an alkyl sulfonate-substituted C1-6 alkyl group, and R7 and R10 represent a C1-6 alkyl group, an aryl group, a C1-6 alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group, and an agent for measurement of nitrogen monoxide containing the compound. Said compound can give a fluorescent substance that is free from change in fluorescence intensity in a wide pH range.Type: GrantFiled: March 21, 2012Date of Patent: March 18, 2014Assignees: Tetsuo Nagano, Daiichi Pure Chemicals Co., Ltd.Inventors: Tetsuo Nagano, Yu Gabe, Yasuteru Urano
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Publication number: 20140072990Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.Type: ApplicationFiled: September 18, 2013Publication date: March 13, 2014Applicants: The University of Tokyo, Mitsubishi Rayon Co., Ltd.Inventors: Yasuteru URANO, Tetsuo NAGANO, Tomoe OHTA, Fujio YU
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Patent number: 8461358Abstract: A compound represented by the formula (I) or a salt thereof (R1 represents hydrogen atom, or a substituent; R2 to R7 represent hydrogen atom, hydroxyl group, an alkyl group, or a halogen atom; R8 and R9 represent hydrogen atom, or an alkyl group; X represents a C1-C3 alkylene group; and R10 represents an acyl group), which promptly causes a structural change from a non-fluorescent closed ring structure to a strongly fluorescent open ring structure by hydrolysis of an acyl group, and is useful as a fluorescent probe for measuring a protease showing superior suitability for quantification.Type: GrantFiled: February 19, 2010Date of Patent: June 11, 2013Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Masayo Sakabe
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Patent number: 8410164Abstract: A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.Type: GrantFiled: May 15, 2009Date of Patent: April 2, 2013Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
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Publication number: 20130065263Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.Type: ApplicationFiled: May 25, 2011Publication date: March 14, 2013Applicants: The University of Tokyo, Mitsubishi Rayon Co., Ltd.Inventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
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Patent number: 8394850Abstract: A compound represented by the following general formula (IA) or (IB) (R1 represents an electron withdrawing substituent, R2 and R3 represent a hydrogen atom or a halogen atom; R4 and R3 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R6 represents a hydrogen atom or an alkyl group) or a salt thereof, and a reagent for measuring hydrogen peroxide comprising the compound or a salt thereof.Type: GrantFiled: March 4, 2009Date of Patent: March 12, 2013Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Masahiro Abo
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Publication number: 20130023675Abstract: An agent for diagnosing cancer comprising, as an active ingredient, a compound represented by the following formula (I) (R1 represents hydrogen atom, or a substituent; R2 to R7 represent hydrogen atom, hydroxyl group, an alkyl group, or a halogen atom; R8 and R9 represent hydrogen atom, or an alkyl group; and X represents a C1-C3 alkylene group), which specifically emits fluorescence in a cancerous tissue within a short time when applied to a part in which presence of a cancerous tissue is suspected.Type: ApplicationFiled: January 12, 2011Publication date: January 24, 2013Applicant: THE UNIVERSITY OF TOKYOInventors: Yasuteru Urano, Tetsuo Nagano, Masayo Sakabe
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Patent number: 8309319Abstract: A fluorescent probe for measurement of UDP-glucuronosyltransferase, which comprises a fluorescein derivative, wherein in the fluorescein derivative, the 2-carboxy group on the benzene ring of fluorescein is replaced with another monovalent substituent, provided that said substituent is a substituent other than sulfo group, and the substituent does not have carboxy group or sulfo group, and wherein the fluorescein derivative may have an arbitrary substituent at a position on the benzene ring other than the 2-position, and the fluorescein derivative may have a substituent selected from the group consisting of an alkoxy group and a halogen atom at the 2-position and/or the 7-position of fluorescein.Type: GrantFiled: February 26, 2008Date of Patent: November 13, 2012Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Rie Tomiyasu
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Patent number: 8258171Abstract: A compound represented by the following general formula (I): wherein R1 represents an amino group which may be substituted with one or two alkyl groups; R2, R3, R4 and R5 independently represent an alkyl group; and R6 and R7 independently represent a monocarboxyalkyl group, a salt thereof, or an ester thereof, which is useful as a pH-sensitive fluorescent probe which emits intense fluorescence in an acidic region.Type: GrantFiled: November 15, 2007Date of Patent: September 4, 2012Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Daisuke Asanuma
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Publication number: 20120178174Abstract: A compound represented by the formula (I) wherein R1 and R2 represent amino groups that substitute at adjacent positions on the benzene ring, wherein one of the amino groups may have one alkyl group; R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group, R5 and R8 independently represent a C1-6 alkyl group, R6 and R9 represent a carboxy-substituted C1-6 alkyl group, an alkoxycarbonyl-substituted C1-6 alkyl group, a sulfo-substituted C1-6 alkyl group, or an alkyl sulfonate-substituted C1-6 alkyl group, and R7 and R10 represent a C1-6 alkyl group, an aryl group, a C1-6 alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group, and an agent for measurement of nitrogen monoxide containing the compound. Said compound can give a fluorescent substance that is free from change in fluorescence intensity in a wide pH range.Type: ApplicationFiled: March 21, 2012Publication date: July 12, 2012Applicants: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Tetsuo NAGANO, Yu GABE, Yasuteru URANO
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Patent number: 8178669Abstract: A compound represented by the following general formula (I): {R1, R3, R6 and R8 are methyl groups and the like, R2 and R7 are cyano groups and the like, R4 and R5 are fluorine atoms and the like, and X is a group represented by the formula (A) [R11 is a monocarboxy-substituted C1-8 alkoxy group and the like, R12, R14 and R15 are hydrogen atoms and the like, and R13 is hydroxy group and the like], provided that the combination of the aforementioned substituents is such a combination that the compound represented by the formula (I) can be substantially non-fluorescent before reacting with peroxynitrite, and the compound represented by the formula (I) in which the group represented by the formula (A) is nitrated after reacting with peroxynitrite can be substantially highly fluorescent}, or a salt thereof, which is useful as a fluorescent probe for peroxynitrite measurement.Type: GrantFiled: November 14, 2006Date of Patent: May 15, 2012Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Tasuku Ueno
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Patent number: 8143069Abstract: A compound represented by the following general formula (II): wherein R21 represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R21 represents two or more substituents, the substituents may be the same or different; R22, R24, R25, and R27 independently represent hydrogen atom, or a monovalent substituent; R23 represents —OH or —N(R28)(R29) (wherein R28 and R29 independently represent an alkyl group which may be substituted); R26 represents —N(R30)(R31) (wherein R30 and R31 independently represent an alkyl group which may be substituted); n represents an integer of 1 to 3; and Y represents —S— or —O—, which is useful for selective measurement of hypochlorite ion.Type: GrantFiled: March 1, 2007Date of Patent: March 27, 2012Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku
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Publication number: 20120052518Abstract: A compound represented by the formula (I) or a salt thereof (R1 represents hydrogen atom, or a substituent; R2 to R7 represent hydrogen atom, hydroxyl group, an alkyl group, or a halogen atom; R8 and R9 represent hydrogen atom, or an alkyl group; X represents a C1-C3 alkylene group; and R10 represents an acyl group), which promptly causes a structural change from a non-fluorescent closed ring structure to a strongly fluorescent open ring structure by hydrolysis of an acyl group, and is useful as a fluorescent probe for measuring a protease showing superior suitability for quantification.Type: ApplicationFiled: February 19, 2010Publication date: March 1, 2012Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Yasuteru Urano, Masayo Sakabe
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Publication number: 20110287552Abstract: A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.Type: ApplicationFiled: December 4, 2009Publication date: November 24, 2011Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
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Publication number: 20110251404Abstract: A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.Type: ApplicationFiled: May 15, 2009Publication date: October 13, 2011Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
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Publication number: 20110165085Abstract: A tumor cell or tumor tissue-selective fluorescent staining agent comprising a compound represented by the following general formula (I): wherein R1 and R2 independently represent a C1-C4 alkyl group which may be substituted, a C2-C4 alkenyl group which may be substituted, a C2-C4 alkynyl group which may be substituted, an aryl group which may be substituted, or a heteroaryl group which may be substituted.Type: ApplicationFiled: February 1, 2011Publication date: July 7, 2011Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo NAGANO, Yasuteru URANO, Hirotatsu KOJIMA, Yuuta FUJIKAWA, Tetsuro TAKAMATSU, Yoshinori HARADA
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Publication number: 20110159603Abstract: A compound represented by the following general formula (IA) or (IB) (R1 represents an electron withdrawing substituent, R2 and R3 represent a hydrogen atom or a halogen atom; R4 and R3 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R6 represents a hydrogen atom or an alkyl group) or a salt thereof, and a reagent for measuring hydrogen peroxide comprising the compound or a salt thereof.Type: ApplicationFiled: March 4, 2009Publication date: June 30, 2011Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Yasuteru Urano, Masahiro Abo