Abstract: Disclosed are methods of isolating paired T cell receptor (TCR) alpha and beta chain sequences, or an antigen-binding portion thereof. Also disclosed are methods of automatically identifying the TCR alpha and beta chain V segment sequences and CDR3 sequences of a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Methods of preparing a population of cells that express paired TCR alpha and beta chain sequences, or an antigen-binding portion thereof, are also disclosed. Isolated pairs of TCR alpha and beta chain sequences and isolated populations of cells prepared by the methods are also disclosed.

Abstract: A cordycepin-based derived compound with an anti-tumor effect, and a structure of the compound is as shown in formula I; a cordycepin derivative and a pharmaceutical composition thereof provided by the invention have a good anti-tumor proliferation effect; compared with a parent drug, the cordycepin derivative has better affinity to cell membranes, so that a half-life period of in-vivo metabolism of the drug is longer, and in-vivo remaining time of the drug is longer; compared with other nucleoside anti-tumor drugs, the cordycepin derivative and the pharmaceutical composition thereof provided by the invention have wider types and action ranges of tumors, have excellent inhibition effects on a gastric cancer, a pancreatic cancer, a liver cancer, a small cell lung cancer, a colorectal cancer, melanoma, an ovarian cancer and the like, and have lower side effects and better curative effects.

Abstract: An automatic door operator includes a lead screw having a first end connected to an output end of an actuating unit and a second end extending away from the output end. A lead screw nut is attached to the lead screw. A slider is attached to the lead screw nut. The slider is provided with a rack portion extending in a direction parallel to an axial direction of the lead screw, and during a movement of the lead screw nut, a length of an overlap of the rack portion and the lead screw in the axial direction varies. The operator includes a housing having a guide hole configured to be slidably engaged with the slider and an output shaft is mounted in a mounting hole disposed on the housing. The output shaft has a gear portion positioned in the guide hole and engaged with the rack portion.

Abstract: A self-learning method for an obstacle and a self-learning method for a new obstacle are provided. With the self-learning method, in step 1, data of a grid map in a movement region is acquired, and each of grids of the grid map is marked as not being traversed. In step 2, a starting grid of the grid map is set, data of the starting grid is pushed into a stack, a movement unit is controlled to move to the starting grid, and the starting grid is remarked as being traversed. In step 3, the movement unit is controlled to traverse the grid map, and boundary data of the obstacle is acquired based on marks of the grids in the grid map. In step 4, boundary data of the obstacle is stored.

Abstract: An automatic door operator and a drive mechanism thereof are disclosed. The drive mechanism of the automatic door includes a planetary reducer driven by an electric motor, and a drive shaft driven by a planetary reducer. The planetary reducer includes a housing and a planetary gear train mounted in the housing. The planetary gear train includes a final-stage planet carrier configured to output torque, and a center of the final-stage planet carrier is provided with a torque output hole. A first end of the drive shaft is detachably inserted in the torque output hole and is non-rotatably connected to the torque output hole, and a second end of the drive shaft extends out of the housing. The drive mechanism of the automatic door operator is advantageous to the miniaturization design of the automatic door operator.

Abstract: Disclosed is an isolated or purified T cell receptor (TCR) having antigenic specificity for mutated human p53. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

Abstract: This disclosure relates to the technical field of automobiles and discloses an automobile key programmer applied to an automobile diagnostic instrument, wherein the automobile key programmer includes an activation coil, a first changeover switch, a second changeover switch, an infrared modulation circuit, an amplitude shift keying modulation circuit, and a frequency shift keying modulation circuit; when the first changeover switch is not powered on, the infrared modulation circuit provides a resonant voltage for the activation coil; when the first changeover switch is powered on and the second changeover switch is not powered on, the amplitude shift keying modulation circuit provides a resonant voltage for the activation coil; when both the first changeover switch and the second changeover switch are powered on, the frequency shift keying modulation circuit provides a resonant voltage for the activation coil; only one coil is required in the automobile key programmer to change voltages.

Abstract: An assembly, an apparatus, and a method for machining a mechanical part. The assembly includes a parallel robot adapted to be mounted onto a platform under the mechanical part to be machined. The assembly includes a servo spindle mounted on the parallel robot and configured to drive a machining tool to rotate. The parallel robot is configured to drive the servo spindle to translate along the one or more axes with respect to the parallel robot. During the machining of the mechanical part, the parallel robot may drive the servo spindle to translate along the one or more axes under the mechanical part, such that the machining tool may cut out the required shapes and characteristics at a bottom side of the mechanical part.

Abstract: Provided are a switching method and apparatus, a device, and a storage medium. The method includes: executing a protection switching operation in response to detecting a switching trigger condition, synchronizing, by a local first-type communication node, protection group switching related state information to a second-type communication node, and synchronously forwarding, by the second-type communication node, the protection group switching related state information of the local first-type communication node to an opposite first-type communication node.

Abstract: An assembly and an apparatus for machining a mechanical part. The assembly includes a parallel robot configured to be mounted onto an end flange of a joint robot. The parallel robot is configured to drive the servo spindle to translate along the one or more axes with respect to the parallel robot. During the machining of the mechanical part, the joint robot can stay stationary for a specific machining position of the mechanical part, and the parallel robot drives the servo spindle to translate along the one or more axes. Then, the machining tool may cut out the required shapes and characteristics at the specific machining position of the mechanical part.

Abstract: A control method for a wireless smart wearable device is performed by a terminal device and includes: receiving a control instruction for the wireless smart wearable device from a preset control interface of the terminal device; determining a target device connected with the wireless smart wearable device; and transmitting, according to the control instruction, corresponding control information to the target device, so as to instruct the target device to control the wireless smart wearable device according to the control information.

Abstract: A connection method for a wireless smart wearable device is performed by a terminal device, and includes: receiving a connection switching instruction of the wireless smart wearable device from a preset control interface of the terminal device; determining a target device according to the connection switching instruction; and instructing, according to a preset parameter of the wireless smart wearable device, the target device to be connected with the wireless smart wearable device according to the preset parameter.

Abstract: Disclosed are methods of isolating paired T cell receptor (TCR) alpha and beta chain sequences, or an antigen-binding portion thereof. Also disclosed are methods of automatically identifying the TCR alpha and beta chain V segment sequences and CDR3 sequences of a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Methods of preparing a population of cells that express paired TCR alpha and beta chain sequences, or an antigen-binding portion thereof, are also disclosed. Isolated pairs of TCR alpha and beta chain sequences and isolated populations of cells prepared by the methods are also disclosed.

Abstract: Disclosed is an isolated or purified T cell receptor (TCR) having antigenic specificity for mutated Kirsten rat sarcoma viral oncogene homolog (KRAS) presented in the context of an HLA-Cw*0802 molecule. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

Abstract: Provided is a method for preparing a hard carbon anode of a lithium/sodium ion battery by biochar, which includes the following steps: S1 biochar pretreatment, S2, preparing a precursor material and S3 pyrolysis carbonization, by a modification of the biochar, setting carbonization temperature and carbonization time, and controlling a cooling rate, so that the obtained biochar generates amorphous carbon after carbonization with low graphitization degree, few surface defects, larger charge-discharge specific capacity, and stable performance in the cycle charging and discharging process; at the same time, the process of the application is relatively simple, and a special complex process is reduced; the present method is suitable for a large-scale industrial production without special adjustment or control of a morphology; and the product performance is relatively good and thus has certain competitive advantages.

Abstract: The present invention discloses a quantum-dot film, wherein the quantum-dot film comprises a binder and a plurality of quantum dots dispersed in the binder, wherein the plurality of quantum dots are capable of being water-resistant and oxygen-resistant.

Abstract: A method for preparing a nitrogen-containing heterocyclic compound and a derivative thereof by an enzymatic-chemical cascade method, comprising: reacting an alcohol, an amine, an alcohol dehydrogenase, a flavin molecule and a coenzyme in a solvent to obtain the nitrogen-containing heterocyclic compound and the derivative thereof; compared with the prior art, the method is a green and economical enzymatic-chemical cascade method, and is used for synthesizing nitrogen-containing heterocyclic compounds and derivatives thereof; compared with a common toxic chemical catalyst, the alcohol dehydrogenase is selected as a catalyst in the method, which has the characteristics of high substrate specificity, no pollution, high catalytic efficiency, no toxic solvents and simple post-treatment.

Abstract: An adjustable platform for installing a wind turbine generator comprises an adjustable platform shell, the adjustable platform shell is installed on a hull, pile legs are connected vertically in the adjustable platform shell in a sliding way, a plurality of adjusting jacks are arranged on the pile legs, an electric plug-in component is arranged in the adjustable platform shell and used for fitting with the adjusting jacks. The fitting of electric plug-in component with the adjusting jacks of the pile legs replaces the bolted connection between the installation platform and the pile legs, and the electric power is used to replace the manual operation, solving the technical problems in the prior art that bolted connection is required between the installation platform and the pile legs, bolts are required to be removed manually before the pile legs sink, and the labor cost is greatly consumed.

Abstract: An improved device for solid phase microextraction, the device including a plastic substrate, a pre-coating layer on the plastic substrate, and an SPME coating on the precoating layer. SPME coatings are typically incompatible with plastic substrates due to differences between the surface energies of the substrate and the coating, leading to uneven coating and poor adhesion. The addition of the precoating layer provides increased evenness and stronger adhesion of the SPME coating to the plastic substrate. Also provided are method of coating an SPME coating on a plastic substrate, the method including the step of precoating the plastic substrate to provide a precoated substrate and then coating the precoated substrate with an SPME coating, wherein precoating provides improved coating evenness and adhesion of the SPME coating to the plastic substrate.