Patents by Inventor Yong Xu

Yong Xu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for altering the cleavage specificity of a type IIG restriction endonuclease
    Publication number: 20040219584
    Abstract: Methods are provided for altering the cleavage specificity of a Type IIG restriction endonuclease, the Type IIG restriction endonuclease being characterized by a cleavage domain adjacent to a methylase domain, the methylase domain located adjacent to a specificity domain. The method includes ligating DNA or protein sequences to form a fusion DNA or fusion protein. Where a fusion DNA is formed, the host cell is transformed with the fusion DNA to express a Type IIG restriction endonuclease with altered cleavage specificity.
    Type: Application
    Filed: March 15, 2004
    Publication date: November 4, 2004
    Inventors: Shuang-yong Xu, Daniela Kobbe, Zhenyu Zhu, James Samuelson
  • Method for cloning and expression of AcuI restriction endonuclease and AcuI methylase in E. coli
    Publication number: 20040209257
    Abstract: The present invention relates to recombinant DNA encoding the AcuI restriction endonuclease as well as AcuI methylase, and expression of AcuI restriction endonuclease and AcuI methylase in E. coli cells containing the recombinant DNA.
    Type: Application
    Filed: April 16, 2003
    Publication date: October 21, 2004
    Inventors: James Samuelson, Shuang-Yong Xu, Diana O'Loane
  • Method for cloning and expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli
    Patent number: 6794172
    Abstract: The present invention relates to recombinant DNA encoding the PpuMI restriction endonuclease as well as PpuMI methylase, expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: May 17, 2002
    Date of Patent: September 21, 2004
    Assignee: New England Biolabs, Inc.
    Inventors: James Samuelson, Shuang-yong Xu
  • Method for cloning and expression of OkrAI restriction endonuclease and methyltransferase
    Publication number: 20040175816
    Abstract: The present invention relates to recombinant DNA encoding the OkrAI restriction endonuclease as well as OkrAI methylase, expression of OkrAI restriction endonuclease in E. coli cells containing the recombinant DNA.
    Type: Application
    Filed: March 3, 2003
    Publication date: September 9, 2004
    Applicant: New England Biolabs, Inc.
    Inventors: Shuang-Yong Xu, Jian-Ping Xiao, Rebecca B. Kucera, James Samuelson
  • Surface-micromachined pressure sensor and high pressure application
    Patent number: 6782755
    Abstract: A surface-micromachined high-pressure sensor, formed by forming a cavity using a sacrificial layer. The sacrificial layer can be reflowed to make the edges of the cavity more rounded. The material that is used for the diaphragm can be silicon nitride, or multiple layers including silicon nitride and other materials. The pressure sensor is intended to be used in high pressure applications, e.g. pressure is higher than 6000, 10,000 or 30,000 P.S.I.
    Type: Grant
    Filed: July 6, 2001
    Date of Patent: August 31, 2004
    Assignees: California Institute of Technology, Schlumberger Technology Corporation
    Inventors: Yu-Chong Tai, Yong Xu, Fukang Jiang
  • Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease
    Patent number: 6764843
    Abstract: The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: July 20, 2004
    Assignee: New England Biolabs, Inc.
    Inventors: Shuang-yong Xu, Andrew Dore, Adam Hume, John Pelletier, Jing Zhou
  • Method for cloning and expression of TTH111II restriction endonuclease-methylase in E. coli
    Publication number: 20040132129
    Abstract: The present invention relates to recombinant DNA which encodes the Tth111II restriction endonuclease-methylase fusion protein (Tth111IIRM), expression of Tth111II restriction endonuclease-methylase fusion protein in E. coli cells containing the recombinant DNA, and purification of Tth111II endonuclease-methylase fusion protein to near homogeneity.
    Type: Application
    Filed: January 8, 2003
    Publication date: July 8, 2004
    Applicant: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Derek Robinson, Jack Benner, Shuang-Yong Xu
  • Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
    Patent number: 6723546
    Abstract: The present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity.
    Type: Grant
    Filed: March 26, 2002
    Date of Patent: April 20, 2004
    Assignee: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Shuang-Yong Xu
  • Method for cloning and expression of MspA1l restriction endonuclease and MspA1l methylase in E. coli
    Patent number: 6673588
    Abstract: The present invention relates to recombinant DNA coding for the MspA1I restriction endonuclease as well as MspA1I methylase, expression of MspA1I restriction endonuclease and MspA1I methylase in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: February 26, 2002
    Date of Patent: January 6, 2004
    Assignee: New England Biolabs, Inc.
    Inventors: Shuang-yong Xu, Robert Maunus, Katy Stropnicky
  • Method for cloning and expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli
    Publication number: 20030215907
    Abstract: The present invention relates to recombinant DNA encoding the PpuMI restriction endonuclease as well as PpuMI methylase, expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli cells containing the recombinant DNA.
    Type: Application
    Filed: May 17, 2002
    Publication date: November 20, 2003
    Applicant: New England Biolabs, Inc.
    Inventors: James Samuelson, Shuang-Yong Xu
  • Integrated circuit-integrated flexible shear-stress sensor skin and method of fabricating the same
    Publication number: 20030199116
    Abstract: An IC-integrated, flexible, shear-stress sensor skin is made by providing a wafer with integrated circuits and sensor elements which are fabricated in the wafer, disposing a first polymer layer on the wafer and sensor elements to provide mechanical support for the sensor elements, defining a cavity below the sensor elements to provide thermal isolation, while the sensor element remains supported by the first polymer layer, and isolating the sensor elements into a plurality of islands defined in the wafer, so that the islands, with at least one sensor element on at least one of the islands, and the integrated circuits form the IC-integrated, flexible, shear-stress sensor skin. The invention is an IC-integrated, flexible, sensor skin made according to the method.
    Type: Application
    Filed: March 31, 2003
    Publication date: October 23, 2003
    Inventors: Yu-Chong Tai, Yong Xu
  • Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
    Publication number: 20030186363
    Abstract: The present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity.
    Type: Application
    Filed: March 26, 2002
    Publication date: October 2, 2003
    Applicant: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Shuang-Yong Xu
  • Methods for forming semiconductor lenses on substrates
    Patent number: 6627469
    Abstract: A lens is formed out of semiconductor material. The semiconductor produces light which is coupled to the lens. The lens focuses the light and also minimizes refractive reflection. The lens is formed by a graded aluminum alloy, which is oxidized in a lateral direction. The oxidation changes the effective shape of the device according to the grading.
    Type: Grant
    Filed: April 10, 2001
    Date of Patent: September 30, 2003
    Assignee: California Institute of Technology
    Inventors: John O'Brien, Chuan-cheng Cheng, Axel Scherer, Amnon Yariv, Yong Xu
  • Method for cloning and expression of Rhodothermus obamensis DNA polymerase I large fragment in E. coli
    Publication number: 20030165890
    Abstract: The present invention provides a novel thermostable DNA polymerase I obtainable from Rhodothermus obamensis, which possesses 3′-5′ exonuclease activity and has a half-life of about 35 minutes at 94° C. This polymerase also contains a tyrosine residue in the ribosome binding site which improves incorporation of dideoxyribonucleic acids. Also provided are isolated DNA and vectors encoding this polymerase, as well as its large fragment, and methods for producing recombinant enzyme using the same.
    Type: Application
    Filed: May 14, 2002
    Publication date: September 4, 2003
    Applicant: New England Biolabs, Inc.
    Inventor: Shuang-Yong Xu
  • METHOD FOR CLONING AND EXPRESSION OF MSPA1I RESTRICTION ENDONUCLEASE AND MSPA1I METHYLASE IN E. COLI
    Publication number: 20030166206
    Abstract: The present invention relates to recombinant DNA coding for the MspA1I restriction endonuclease as well as MspA1I methylase, expression of MspA1I restriction endonuclease and MspA1I methylase in E. coli cells containing the recombinant DNA.
    Type: Application
    Filed: February 26, 2002
    Publication date: September 4, 2003
    Applicant: New England Biolabs, Inc.
    Inventors: Shuang-Yong Xu, Robert Maunus, Katy Stropnicky
  • Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli
    Patent number: 6596524
    Abstract: The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.
    Type: Grant
    Filed: September 20, 2001
    Date of Patent: July 22, 2003
    Assignee: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Jing Zhou, Shuang-yong Xu
  • Method for cloning and expression of BseRI restriction endonuclease and BseRI methylase in E. coli
    Patent number: 6593122
    Abstract: The present invention relates to recombinant DNA that encodes the BseRI restriction endonuclease as well as M.BseRI, expression of BseRI restriction endonuclease and M.BseRI in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: October 11, 2001
    Date of Patent: July 15, 2003
    Assignee: New England Biolabs, Inc.
    Inventors: Shuang-yong Xu, Robert Maunus, Jack Benner
  • Method for cloning and expression of TspRI restriction endonuclease and TspRI methylase in E. coli
    Patent number: 6589769
    Abstract: The present invention relates to recombinant DNA that encodes the TspRI restriction endonuclease as well as TspRI methylase, expression of TspRI restriction endonuclease and TspRI methylase in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: October 19, 2001
    Date of Patent: July 8, 2003
    Assignee: New England Biolabs, Inc.
    Inventors: Shuang-yong Xu, Andrew Dore, Michael Dalton, Jack Benner
  • Method for cloning and expression of BsaWI restriction endonuclease and BsaWI methylase in E. coli
    Patent number: 6586220
    Abstract: The present invention relates to recombinant DNA encoding the BsaWI restriction endonuclease as well as BsaWI methylase, expression of BsaWI restriction endonuclease and BsaWI methylase in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: February 26, 2002
    Date of Patent: July 1, 2003
    Assignee: New England Biolabs, Inc.
    Inventors: Shuang-yong Xu, Jing Zhou, Adam Hume, Robert Maunus
  • Method for cloning and expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli
    Publication number: 20030104388
    Abstract: The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.
    Type: Application
    Filed: September 20, 2001
    Publication date: June 5, 2003
    Applicant: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Jing Zhou, Shuang-Yong Xu