Abstract: [Problems] To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency. [Means for Solving Problems] Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.

Abstract: The present invention effectively produces glucose dehydrogenase derived from Aspergillus oryzae, and provides more practical glucose dehydrogenase.

Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.

Abstract: The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.

Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.

Abstract: A method of quantifying glucose in a solution characterized in that electric potential measurement is conducted by potentiometry using a glucose dehydrogenase that requires a flavin compound as a coenzyme. It is preferable to carry out the quantification using a glucose dehydrogenase derived from a filamentous fungus, in particular derived from Aspergillus oryzae or Aspergillus terreus.

Abstract: Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3?-5? exonuclease activity, histidine(H) has been replaced by another amino acid.

Abstract: The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Abstract: The present invention provides composite particles which have magnetism and simultaneously emit fluorescence with a variety of wavelengths, and which are suitable for use in the fields of biology, biochemistry or the like. The composite particles of the present invention comprise ferromagnetic iron oxide particles, fluorescent pigment particles and silica, and have an average particle size of 1 to 10 ?m, a coercive force of 2.39 to 11.94 kA/m (30 to 150 oersted), saturation magnetization of 0.5 to 40 A.m2/kg (0.5 to 40 emu/g). The peak value of the wavelength of fluorescence from the composite particle is in the range of 350 to 750 nm, when the composite particle is excited by light with a wavelength of 250 to 600 nm.

Abstract: The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

Abstract: [Problems] To provide a method which comprises detecting phosphorylation of substrate peptides with a quick and easy scheme using an inexpensive substance without a need for a special technique and which among other things enables comprehensive profiling kinetics of various protein kinases. [Means for Solving Problems] A method for analysis of protein kinase activity by detecting phosphorylation of at least one peptide which serves as a substrate for at least one protein kinase and is immobilized on the metal thin film in a basal plate of an array, characterized in that, in detecting the phosphorylation, the phosphorylated peptide is treated with a polyamine-zinc complex represented by, e.g., formula (I) which has a molecular weight of 500 to 1,000 and is modified with biotin and then preferably with avidin or streptavidin.

Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.

Abstract: The present invention effectively produces glucose dehydrogenase derived from Aspergillus oryzae, and provides more practical glucose dehydrogenase. The invention makes it possible to efficiently produce glucose dehydrogenase and to obtain glucose dehydrogenase in more practical manner by using a glucose dehydrogenase gene isolated from Aspergillus oryzae.

Abstract: The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.

Abstract: The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

Abstract: The present invention provides a device relating to separation (extraction, purification) of a biological component such as nucleic acid, protein and the like from a liquid sample containing the biological component, and, as a method, a device for separating a biological component, which contains magnetically responsive particles and a chip obtained by adhering a pair of substrates, which contains one or multiple grooves formed on at least one surface thereof, with the groove(s) placed inside, and a method of separating a biological component from a liquid sample, which uses this device, and includes the following steps (a)-(d): (a) a step of holding the device such that the adhesion surface of the pair of substrates is about perpendicular to the horizontal direction, (b) a step of adsorbing the biological component to magnetically responsive particles by contacting the magnetically responsive particles with the liquid sample containing the biological component, (c) a step of separating the magnetically respo

Abstract: The present invention provides composite particles which have magnetism and simultaneously emit fluorescence with a variety of wavelengths, and which are suitable for use in the fields of biology, biochemistry or the like. The composite particles of the present invention comprise ferromagnetic iron oxide particles, fluorescent pigment particles and silica, and have an average particle size of 1 to 10 ?m, a coercive force of 2.39 to 11.94 kA/m (30 to 150 oersted), saturation magnetization of 0.5 to 40 A.m2/kg (0.5 to 40 emu/g). The peak value of the wavelength of fluorescence from the composite particle is in the range of 350 to 750 nm, when the composite particle is excited by light with a wavelength of 250 to 600 nm.

Abstract: A method for purifying nucleic acids or proteins comprising a plurality of piston pumps; and a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable. The apparatus includes a mechanism for mixing a liquid mixture of each sample and a reagent in a plurality of sections of a container by sucking up a plurality of the mixtures into tips, followed by discharging the mixtures in the sections simultaneously. The apparatus also includes a mechanism for dispensing a desired amount of a reagent to be used subsequently into the same number of sections of a different container as samples, while the mixing is in progress.

Abstract: A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine+H2O?sarcosine+urea Optimum temperature: about 40-50° C. Optimum pH: pH about 8.0-9.0 Heat stability: not more than 50° C. (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.00 mM Molecular weight: about 43,000 (SDS-PAGE) Isoelectric point: 3.5 4.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.