Abstract: A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy??(1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3.

Abstract: The present invention provides a strikingly convenient novel purification method of protein, as compared to conventional methods, which affords automation and high throughput, and a material therefor. That is, the present invention provides a magnetic carrier containing a ferromagnetic oxide particle and a carbohydrate layer coating the ferromagnetic oxide particle, a purification method of protein using the carrier, and a reagent kit for purification of protein.

Abstract: The present invention mainly provides a magnetic carrier for biological substance, which shows improved dispersibility in aqueous solutions and is superior in the collectability by the magnetic field, reversible binding ability with a biological substance, elution property of the bound biological substance, and isolation and purification efficiency of biological substance, as compared to conventional magnetic carriers. The magnetic carrier of the present invention includes a magnetic carrier having a saturation magnetization of 10-80 A·m2/kg and a coercive force of 0.80-15.92 kA/m, a magnetic carrier wherein a ferromagnetic iron oxide particle is coated with a compound comprising silicon and aluminum, and the like.

Abstract: The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.

Abstract: The present invention provides a strikingly convenient novel purification method of protein, as compared to conventional methods, which affords automation and high throughput, and a material therefor. That is, the present invention provides a magnetic carrier containing a ferromagnetic oxide particle and a carbohydrate layer coating the ferromagnetic oxide particle, a purification method of protein using the carrier, and a reagent kit for purification of protein.

Abstract: The present invention provides an apparatus for purifying nucleic acids such as DNA and RNA or proteins such as enzymes and antibodies from microorganisms such as viruses and bacteria or animal and plant tissues, the apparatus being capable of fast processing of a plurality of samples.

Abstract: The present invention provides an apparatus for purifying nucleic acids such as DNA and RNA or proteins such as enzymes and antibodies from microorganisms such as viruses and bacteria or animal and plant tissues, the apparatus being capable of fast processing of a plurality of samples.

Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

Abstract: A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H.sub.2 O.fwdarw.sarcosine+ureaOptimum temperature: about 40-50.degree. C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50.degree. C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: about 3.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.

Abstract: A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No. 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

Abstract: A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No: 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

Abstract: A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine+H2O?sarcosine+urea Optimum temperature: about 40-50° C. Optimum pH: pH about 8.0-9.0 Heat stability: not more than about 50° C. (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mM Molecule weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5 4.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.