Abstract: Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

Abstract: A method is disclosed for obtaining the 5?ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5?ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5? ends can be used for characterization and cloning of the transcriptome.

Abstract: Abstract of the Disclosure A printed material comprising at least one support having at least one oligomer and/or polymer applied thereon is provided. Also, a method for preparing the printed material and a method for delivering and storing at least one oligomer and/or polymer are provided. The printed materials of the present invention are useful in providing scientists with oligomers and/or polymers of interest from the printed materials easily and immediately.

Abstract: The invention discloses a family of cloning vectors capable of cloning nucleic acid inserts of interest of long sizes, with low or reduced background and high efficiency of excision and method for preparing these vectors and library thereof. As example, it is disclosed a cloning vector comprising a construction vector segment (CS) and a replaceable segment (RS), wherein the size of CS is: 36.5 kb?CS<38 kb, preferably CS is 37.5 kb, comprising lox recombination sites for Cre-recombination and/or att recombination sites for Gateway-like recombination, preferably also a background-reducing system selected from the group of: the ccdB gene, a lox sequence, the lacZ gene, and asymmetric site sequences recognized by restriction endonucleases.

Abstract: Disclosed are RNA polymerases consisting of a wild type RNA polymerase provided that at least one of amino acids in the wild type RNA polymerase has been modified to enhance its ability for incorporating 3?-deoxyribonucleotides and derivatives thereof in comparison with the corresponding wild type RNA polymerases. Specifically, disclosed are, for example, the RNA polymerases wherein at least one amino acid present in a nucleotide binding sites of the wild type RNA polymerases such as phenylalanine has been replaced with tyrosine. The RNA polymerases of the present invention are a RNA polymerase which exhibits little or no bias for incorporation between ribonucleotides and 3?-deoxyribonucleotide as well as among ribonucleotides having different base groups and among deoxyribonucleotides having. different base groups.

Abstract: A method for storing and/or delivering an oligomer and/or polymer applied on at least one support, comprising the steps of: (a) applying at least one oligomer and/or polymer on at least one support; (b) folding or rolling the support; and (c) storing and/or delivering the support of step (b) is provided. Also, a support having at least one oligomer and/or polymer applied thereon, which is in the form of a folded or rolled sheet, a loose-leaf sheet or a card and a water-soluble support having at least one oligomer and/or polymer applied thereon are provided. According to the present invention, molecular substances (oligomers and/or polymers) can be stored and delivered in a simple system. This system can preserve the substances without contamination and degradation.

Abstract: A method of preparing an electrophoretic support comprising washing of at least a portion of the surface of a silicon-containing support member supporting an electrophoretic matrix with a weak alkali solution and supporting of said matrix by said support member; an electrophoretic gel comprising a polyacrylamide polymer obtained by polymerizing acrylamide or a derivative thereof in the presence of two or more polar organic solvents; a method of electrophoresis employing a gel prepared by said preparation method; and a method of electrophoresis employing said electrophoretic gel.

Abstract: A printed material comprising at least one support having at least one oligomer and/or polymer applied thereon is provided. Also, a method for preparing the printed material and a method for delivering and storing at least one oligomer and/or polymer are provided. The printed materials of the present invention are useful in providing scientists with oligomers and/or polymers of interest from the printed materials easily and immediately.

Abstract: It becomes possible to simultaneously atomize and ionize atoms constituting a polymer with the use of a single laser, thereby highly simplifying the constitution of a system. A method of analyzing a polymer which comprises abrading the polymer to be analyzed by irradiating with laser beams to thereby atomize the polymer into the constituting elements thereof, then ionizing the elements and analyzing the constituting elements thus ionized. The laser beams with which the polymer to be analyzed is irradiated for the abrasion are ultrashort pulse laser beams. By irradiating the polymer with the ultrashort pulse laser beams to thereby abrade, the polymer can be atomized and ionized at the same time. Then the thus ionized constituting elements are analyzed.

Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Abstract: The present invention provides a device and a method for efficiently determining an exon-intron junction with high accuracy. The device of the invention is useful for determining an exon-intron junction in a gene region of the genome. This device comprises an input part in which data on a cDNA of organism 1 and the corresponding gene region of organism 2 are input; an operation part in which two non-overlapped sequences i and j, each having at least 10 bases, are extracted from the gene region of organism 2, and, with respect to sequences i and j extracted, s(i, j) defined by s(i, j)=s(x, yij)−C{(b1−j)+(i−a2)−(B1−A2)}2 is calculated; a junction determination part in which a combination of sequences i and j that maximizes s(i, j) is selected; and an output part in which the position of the exon-intron junction determined is output.

Abstract: A capillary cassette is made up of two separated holders, an array of capillaries and a tube. The capillaries that make up the array are inserted into the first holder, extend through second holder having a detection window and then the capillary array is aligned and then bundled and inserted into a heat shrinkable tube which covers positions to which filler is applied, the tube is heated and shrunk to bundle them in an airtight manner and inserted into a mounting member.

Abstract: A linker or population of linkers comprising an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides comprising said linker or population of linkers and a target first strand polynucleotide bound to said linker. A method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. Provided is a linker instead of G tailing, which can be utilized in a method of preparing a cDNA library, and a method of preparing a cDNA library using said linker.

Abstract: The present invention is related to a method for MALDI-TOF-MS analysis and/or sequencing of oligoribonucleotides. The present invention is also related to a method for determining the DNA nucleotide sequence using MALDI-TOF-MS, and a method for determining polymorphism using MALDI-TOF-MS. The present invention provides a kit for analyzing and/or sequencing a DNA template or a RNA transcription product and/or for determining polymorphism by MALDI-TOF-MS.

Abstract: Aptamers are nucleic acids and similar molecules, such as peptide-nucleic acids, that specifically bind to a ligand such as a protein or peptide. The present invention provides aptamers comprising at least one base capable of base pairing and different from the standard Watson-Crick bases. The present invention also relates to a method for preparation of such aptamers and to methods for sequencing nucleic acids that comprise at least one base capable of base pairing and different from the standard Watson-Crick bases.

Abstract: It becomes possible to simultaneously atomize and ionize atoms constituting a polymer with the use of a single laser, thereby highly simplifying the constitution of a system. A method of analyzing a polymer which comprises abrading the polymer to be analyzed by irradiating with laser beams to thereby atomize the polymer into the constituting elements thereof, then ionizing the elements and analyzing the constituting elements thus ionized. The laser beams with which the polymer to be analyzed is irradiated for the abrasion are ultrashort pulse laser beams. By irradiating the polymer with the ultrashort pulse laser beams to thereby abrade, the polymer can be atomized and ionized at the same time. Then the thus ionized constituting elements are analyzed.

Abstract: Novel polynucleotides and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including diagnostic applications, and therapeutic agent screening applications, as well as in treatment of a variety of disease conditions. Also provided are methods of modulating a biological activity of a subject polypeptide and methods of treating disease conditions associated therewith, particularly by administering modulators of the subject polypeptides.

Abstract: An object of the present invention is to find novel genes that relate to, but are not limited to, pain, such as neuropathic pain, shingles pain, and post-herpetic neuralgia, and are induced upon pain, and provide a reagent, method and pharmaceutical for diagnosing and assessing pain, or preventing and treating pain.

Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.

Abstract: An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof. A method of sequencing DNA in which nucleic acid transcripts are obtained using an RNA polymerase and a DNA fragment as a template, the resulted nucleic acid transcripts are separated, the nucleic acid sequence is determined from the separated fractions wherein the nucleic acid transcription reaction is carried out in the presence of a compound selected from a group of compounds represented by the above formula (I). The polyamine compounds above have outstanding accelerating activity on transcription activity of RNA polymerase. Therefore, use of the polyamine compounds in a DNA sequencing method using RNA polymerase can make a length of DNA sequence that can be determined in one sequencing longer.