Abstract: A present micro array manufacturing apparatus includes a substrate placement portion 11 on which a plurality of substrates can be arranged, a tighter plate 12 for storing a solution including a living body sample, a solution storing member 52 for taking in the solution from the tighter plate 12 and storing the solution therein, and a needle 51 which can be inserted into the solution storing member 52 to allow the solution stored in the solution storing member 52 to adhere onto each of the substrates by a given quantity, wherein the solution storing member 52 and needle 51 are moved in directions where they approach and part away from the substrates to thereby form spots on the substrates. And, the individual operations of the solution storing member 52 and needle 51 are carried out by single drive means 32.

Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.

Abstract: An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof.

Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Abstract: A method of preparing labeled RNA probe by reacting RNA polymerase in the presence of a DNA fragment comprising a promoter sequence for the RNA polymerase and substrates of the RNA polymerase. In the method, at least one of said substrates comprises said label, and said RNA polymerase is mutant RNA polymerase where at least one of the amino acids of wild type RNA polymerase has been modified to permit incorporation of the substrate having a label or to improve the incorporation of the substrate having a label. A method of detecting targeted nucleic acid in which targeted nucleic acid and labeled RNA probe prepared by the above method are mixed and RNA probe that has hybridized with the targeted nucleic acid is selectively detected. A kit for preparing labeled RNA probe comprising (1) RNA polymerase, (2) DNA comprising a promoter sequence for said RNA polymerase, (3) substrates of said RNA polymerase, and (4) optionally an instruction manual.

Abstract: A data processing part comprises a time-series data production part for producing time-series data as to each capillary column from a scan waveform obtained by an optical measuring part, further comprises a correction data storage part for storing correction data indicating the relation between the number of data points of saturated parts and light intensity data obtained on the assumption that a peak is unsaturated as to a saturated scan waveform peak and a saturated data correction part obtaining light intensity data as to the saturated peak included in the scan waveform on the basis of the correction data stored in the correction data storage part, and employs the light intensity data obtained by the saturated data correction part as the time-series data.

Abstract: Disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, for example, fluorescent groups, and having a 2′, 3′-dideoxyribonucleotide residue or a 3′-deoxyribonucleotide residue. These compounds can be used as terminators for the chain terminator method. The two kinds of reporters are arranged with a distance sufficient for causing energy transfer from the donor to the acceptor. Also disclosed are methods for determining DNA sequences based on the chain terminator method wherein the chain termination reaction is performed by using the above terminators. Also disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, which can be used as a primer or an initiator in methods for determining DNA sequences utilizing the chain terminator method, and methods for determining DNA sequences utilizing the compounds.

Abstract: A multi-capillary electrophoresis apparatus arranged with a fixed sample injection holder opposite a detection side holder within the same plane and an epi-optical detection system. A sample is injected sequentially and separated components are successively fed to the detection part for analysis by fluorescence. The epi-optical system adjusts the parallelism between the detection side holder and scanning axis of the detector. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.

Abstract: A method for enhancing activity of enzyme at an elevated temperature which comprises adding a substance exhibiting chaperone function such as a saccharide to a reaction mixture containing the enzyme. The method can improve activity of enzymes more easily and more effectively and hence afford increased enzyme activity at an elevated temperature.

Abstract: A method for enhancing activity of enzyme at an elevated temperature which comprises adding a substance exhibiting chaperone function such as a saccharide to a reaction mixture containing the enzyme. The method can improve activity of enzymes more easily and more effectively and hence afford increased enzyme activity at an elevated temperature.

Abstract: A sample is separated and separated components thereof are successively fed to a part to be detected. A laser beam of at least 600 nm from a laser beam source of an optical measuring part is applied to the part to be detected through a dichroic mirror and a lens, for making a fluorochrome bonded to the separated components absorb multiphotons, exciting the fluorochrome and making the same fluoresce. The optical measuring part captures the fluorescence so that photomultipliers detect fluorescence of not more than 510 nm in wavelength, fluorescence longer than 510 nm and not more than 560 nm in wavelength, fluorescence longer than 560 nm and not more than 580 nm in wavelength and fluorescence longer than 580 in wavelength respectively. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.

Abstract: A method of preparing normalized and/or subtracted cDNA; a method in which the cDNA that is normalized and/or subtracted is in the form of uncloned cDNA (cDNA tester); a method of preparing normalized and/or subtracted cDNA comprising the steps of: (a) preparing cDNA tester; (b) preparing normalization and/or subtraction RNA driver; (c) conducting normalization and/or subtraction in two steps in any order, or conducting normalization/subtraction as a single step and mixing the normalization/subtraction RNA driver with said cDNA tester; (d) adding an enzyme capable of cleaving single strand sites on RNA drivers nonspecifically bound to cDNA tester; (e) removing said single strand RNA driver cleaved in step d) from the tester and removing tester/driver hybrids; and (f) recovering the normalized and/or subtracted cDNA; and a method of efficiently preparing normalized and/or subtracted long-chain, full-coding, and full-length cDNA libraries are provided.

Abstract: A holder for a capillary cassette closes a chamber, and is fixed to a detection side holder fixing member. An acidic solution container, an alkaline solution container, a pure water container and a drain container are arranged on a reservoir stage having a dry chamber. A holder up/down mechanism and a stage moving mechanism successively bring an end of a capillary array into contact with an acidic solution, an alkaline solution, pure water and nitrogen gas, and the chamber is decompressed for successively introducing these into capillary columns and performing pretreatment. Thereafter a gel container is arranged in the chamber, which in turn is pressurized for charging the capillary columns with a gel.

Abstract: A drain joint of a pump block is opened, a piston (19a) is pushed while a piston (13a) is fixed, for charging a passage between a Luer-Lok joint (17) and an intersection part as well as a passage between the intersection part and the drain joint with a buffer. Thereafter the piston (13a) is pushed while the piston (19a) is fixed, to charge a passage (7a) with a polymer. Then, the drain joint is closed, the piston (13a) is pushed and the piston (19a) is pulled in response to the amount of pushing, for charging the passage between the intersection point and the Luer-Lok joint (17) with the polymer. Thereafter, the piston (13a) is pushed while the piston (19a) is fixed, to charge a capillary column with the polymer.

Abstract: A method for preparing a CDNA from a mRNA using a reverse transcriptase wherein reverse transcription is performed at a temperature at which the mRNA does not take a secondary structure, for example, at a temperature of 45° C. or more. The method is performed, for example, using a heat-labile reverse transcriptase in the presence of a substance exhibiting chaperone function having chaperone function such as saccharides. The method is performed, for example, in the presence of metal ions necessary for activation of the reverse transcriptase and a chelating agent for the metal ions such as a deoxynucleotide triphosphate. The method is capable of reverse transcription over the full length of mRNA template even if the mRNA is a long chain mRNA and, as a result, producing a full length cDNA.

Abstract: Disclosed are methods for determining DNA nucleotide sequences comprising reacting ribonucleoside 5′-triphosphates and 3′-dNTP derivatives in the presence of an RNA polymerase modified so as to enhance its ability for incorporating the 3′-dNTP derivatives and a DNA fragment containing a promoter sequence for the RNA polymerase to obtain a nucleic acid transcription product, separating the resulting nucleic acid transcription product, and determining a nucleic acid sequence from the resulting separated fraction. These methods can produce a transcription product of a long chain and afford more accurate sequence data where fluctuation of signals from labeled deoxyribonucleotides is reduced.

Abstract: A method of preparing an electrophoretic support comprising washing of at least a portion of the surface of a silicon-containing support member supporting an electrophoretic matrix with a weak alkali solution and supporting of said matrix by said support member; an electrophoretic gel comprising a polyacrylamide polymer obtained by polymerizing acrylamide or a derivative thereof in the presence of two or more polar organic solvents; a method of electrophoresis employing a gel prepared by said preparation method; and a method of electrophoresis employing said electrophoretic gel.

Abstract: Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

Abstract: Disclosed is a method for producing double-stranded DNA comprising treating double-stranded DNA having a homopolymer part or parts at one or both ends with a restriction enzyme to partly or fully eliminate at least one of the homopolymer part or parts. The restriction enzyme is capable of cleaving double-stranded DNA at a cleavage site separate from a recognition site therefor. Disclosed is a method for determining a nucleotide sequence of double-stranded DNA utilizing one or both strands of the double-stranded DNA as a template, wherein the double-stranded DNA used as the template is double-stranded DNA prepared by the above method of the present invention.

Abstract: A method for isolating DNA contained in a biological sample, including: lysing a DNA-containing biological sample and forming a DNA-bound carrier by placing a lysing solution, including the DNA-containing biological sample, a salt, and a cationic surfactant, and having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, into contact with a DNA-binding carrier to bind DNA to the DNA-binding carrier to form the DNA-bound carrier; separating the DNA-bound carrier from other components; dissociating the bound DNA from the DNA-binding carrier; and recovering dissociated DNA. By the method, DNA purified with no preliminary treatment of a biological sample can be recovered at a high yield.