PEPPER HYBRID PS09930066

Abstract

The invention provides seed and plants of pepper hybrid PS09930066 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of pepper hybrid PS09930066 and the parent lines thereof, and to methods for producing a pepper plant produced by crossing such plants with themselves or with another pepper plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Description

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, more specifically, to the development of pepper hybrid PS09930066 and the inbred pepper lines SLR113-1074 and SBY99-1238.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits in a single variety/hybrid. Such desirable traits may include any trait deemed beneficial by a grower and/or consumer, including greater yield, resistance to insects or disease, tolerance to environmental stress, and nutritional value.

Breeding techniques take advantage of a plant's method of pollination. There are two general methods of pollination: a plant self-pollinates if pollen from one flower is transferred to the same or another flower of the same plant or plant variety. A plant cross-pollinates if pollen comes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over many generations become homozygous at almost all gene loci and produce a uniform population of true breeding progeny, a homozygous plant. A cross between two such homozygous plants of different genotypes produces a uniform population of hybrid plants that are heterozygous for many gene loci. Conversely, a cross of two plants each heterozygous at a number of loci produces a population of hybrid plants that differ genetically and are not uniform. The resulting non-uniformity makes performance unpredictable.

The development of uniform varieties requires the development of homozygous inbred plants, the crossing of these inbred plants, and the evaluation of the crosses. Pedigree breeding and recurrent selection are examples of breeding methods that have been used to develop inbred plants from breeding populations. Those breeding methods combine the genetic backgrounds from two or more plants or various other broad-based sources into breeding pools from which new lines and hybrids derived therefrom are developed by selfing and selection of desired phenotypes. The new lines and hybrids are evaluated to determine which of those have commercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a pepper plant of the hybrid designated PS09930066, the pepper line SLR113-1074 or pepper line SBY99-1238. Also provided are pepper plants having all the physiological and morphological characteristics of such a plant. Parts of these pepper plants are also provided, for example, including pollen, an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 comprising an added heritable trait is provided. The heritable trait may comprise a genetic locus that is, for example, a dominant or recessive allele. In one embodiment of the invention, a plant of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 is defined as comprising a single locus conversion. In specific embodiments of the invention, an added genetic locus confers one or more traits such as, for example, herbicide tolerance, insect resistance, disease resistance, and modified carbohydrate metabolism. In further embodiments, the trait may be conferred by a naturally occurring gene introduced into the genome of a line by backcrossing, a natural or induced mutation, or a transgene introduced through genetic transformation techniques into the plant or a progenitor of any previous generation thereof. When introduced through transformation, a genetic locus may comprise one or more genes integrated at a single chromosomal location.

The invention also concerns the seed of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238. The pepper seed of the invention may be provided as an essentially homogeneous population of pepper seed of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238. Essentially homogeneous populations of seed are generally free from substantial numbers of other seed. Therefore, seed of hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 may be defined as forming at least about 97% of the total seed, including at least about 98%, 99% or more of the seed. The seed population may be separately grown to provide an essentially homogeneous population of pepper plants designated PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238.

In yet another aspect of the invention, a tissue culture of regenerable cells of a pepper plant of hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 is provided. The tissue culture will preferably be capable of regenerating pepper plants capable of expressing all of the physiological and morphological characteristics of the starting plant, and of regenerating plants having substantially the same genotype as the starting plant. Examples of some of the physiological and morphological characteristics of the hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 include those traits set forth in the tables herein. The regenerable cells in such tissue cultures may be derived, for example, from embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers, seed and stalks. Still further, the present invention provides pepper plants regenerated from a tissue culture of the invention, the plants having all the physiological and morphological characteristics of hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238.

In still yet another aspect of the invention, processes are provided for producing pepper seeds, plants and fruit, which processes generally comprise crossing a first parent pepper plant with a second parent pepper plant, wherein at least one of the first or second parent pepper plants is a plant of pepper line SLR113-1074 or pepper line SBY99-1238. These processes may be further exemplified as processes for preparing hybrid pepper seed or plants, wherein a first pepper plant is crossed with a second pepper plant of a different, distinct genotype to provide a hybrid that has, as one of its parents, a plant of pepper line SLR113-1074 or pepper line SBY99-1238. In these processes, crossing will result in the production of seed. The seed production occurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing” comprises planting seeds of a first and second parent pepper plant, often in proximity so that pollination will occur for example, mediated by insect vectors. Alternatively, pollen can be transferred manually. Where the plant is self-pollinated, pollination may occur without the need for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first and second parent pepper plants into plants that bear flowers. A third step may comprise preventing self-pollination of the plants, such as by emasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination between the first and second parent pepper plants. Yet another step comprises harvesting the seeds from at least one of the parent pepper plants. The harvested seed can be grown to produce a pepper plant or hybrid pepper plant.

The present invention also provides the pepper seeds and plants produced by a process that comprises crossing a first parent pepper plant with a second parent pepper plant, wherein at least one of the first or second parent pepper plants is a plant of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238. In one embodiment of the invention, pepper seed and plants produced by the process are first generation (F₁) hybrid pepper seed and plants produced by crossing a plant in accordance with the invention with another, distinct plant. The present invention further contemplates plant parts of such an F₁ hybrid pepper plant, and methods of use thereof. Therefore, certain exemplary embodiments of the invention provide an F₁ hybrid pepper plant and seed thereof.

In still yet another aspect, the present invention provides a method of producing a plant derived from hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238, the method comprising the steps of: (a) preparing a progeny plant derived from hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238, wherein said preparing comprises crossing a plant of the hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 with a second plant; and (b) crossing the progeny plant with itself or a second plant to produce a seed of a progeny plant of a subsequent generation. In further embodiments, the method may additionally comprise: (c) growing a progeny plant of a subsequent generation from said seed of a progeny plant of a subsequent generation and crossing the progeny plant of a subsequent generation with itself or a second plant; and repeating the steps for an additional 3-10 generations to produce a plant derived from hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238. The plant derived from hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 may be an inbred line, and the aforementioned repeated crossing steps may be defined as comprising sufficient inbreeding to produce the inbred line. In the method, it may be desirable to select particular plants resulting from step (c) for continued crossing according to steps (b) and (c). By selecting plants having one or more desirable traits, a plant derived from hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 is obtained which possesses some of the desirable traits of the line/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method of producing food or feed comprising: (a) obtaining a plant of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238, wherein the plant has been cultivated to maturity, and (b) collecting one or more peppers from the plant.

In still yet another aspect of the invention, the genetic complement of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 is provided. The phrase “genetic complement” is used to refer to the aggregate of nucleotide sequences, the expression of which sequences defines the phenotype of, in the present case, a pepper plant, or a cell or tissue of that plant. A genetic complement thus represents the genetic makeup of a cell, tissue or plant, and a hybrid genetic complement represents the genetic make up of a hybrid cell, tissue or plant. The invention thus provides pepper plant cells that have a genetic complement in accordance with the pepper plant cells disclosed herein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles, and by the expression of phenotypic traits that are characteristic of the expression of the genetic complement, e.g., isozyme typing profiles. It is understood that hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 could be identified by any of the many well known techniques such as, for example, Simple Sequence Length Polymorphisms (SSLPs) (Williams et al., 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858, specifically incorporated herein by reference in its entirety), and Single Nucleotide Polymorphisms (SNPs) (Wang et al., 1998).

In still yet another aspect, the present invention provides hybrid genetic complements, as represented by pepper plant cells, tissues, plants, and seeds, formed by the combination of a haploid genetic complement of a pepper plant of the invention with a haploid genetic complement of a second pepper plant, preferably, another, distinct pepper plant. In another aspect, the present invention provides a pepper plant regenerated from a tissue culture that comprises a hybrid genetic complement of this invention.

In still yet another aspect, the invention provides a method of determining the genotype of a plant of pepper hybrid PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 comprising detecting in the genome of the plant at least a first polymorphism. The method may, in certain embodiments, comprise detecting a plurality of polymorphisms in the genome of the plant. The method may further comprise storing the results of the step of detecting the plurality of polymorphisms on a computer readable medium. The invention further provides a computer readable medium produced by such a method.

Any embodiment discussed herein with respect to one aspect of the invention applies to other aspects of the invention as well, unless specifically noted.

The term “about” is used to indicate that a value includes the standard deviation of the mean for the device or method being employed to determine the value. The use of the term “or” in the claims is used to mean “and/or” unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive. When used in conjunction with the word “comprising” or other open language in the claims, the words “a” and “an” denote “one or more,” unless specifically noted otherwise. The terms “comprise,” “have” and “include” are open-ended linking verbs. Any forms or tenses of one or more of these verbs, such as “comprises,” “comprising,” “has,” “having,” “includes” and “including,” are also open-ended. For example, any method that “comprises,” “has” or “includes” one or more steps is not limited to possessing only those one or more steps and also covers other unlisted steps. Similarly, any plant that “comprises,” “has” or “includes” one or more traits is not limited to possessing only those one or more traits and covers other unlisted traits.

Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and any specific examples provided, while indicating specific embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants, seeds and derivatives of pepper hybrid PS09930066, pepper line SLR113-1074 and pepper line SBY99-1238. The hybrid PS09930066 is produced by the cross of parent lines SLR113-1074 and SBY99-1238. The parent lines show uniformity and stability within the limits of environmental influence. By crossing the parent lines, uniform seed hybrid PS09930066 can be obtained.

Pepper hybrid PS09930066 develops a large, robust plant that produces a late, continuous set of fruit. The plant produces a very productive set of uniform, medium green maturing to medium red, half-long fruit. The mostly four-lobe, smooth fruit are trapezoidal in shape. Hybrid PS09930066 is resistant to Tobamo virus (P0), bacterial leaf spot (BLS; races 0-3, 7, 8; caused by Xanthomonas campestris pv. vesicatoria), Tomato spotted wilt virus (TSWV; P0), Pepper mottle virus (PepMoV), and Potato virus Y (PVY 1.2). Hybrid PS09930066 is intended for open field production in Mexico and California. It is particularly well adapted to the coastal valleys of California and similar environments.

The development of pepper hybrid PS09930066 and its parent lines can be summarized as follows.

A. Origin and Breeding History of Pepper Hybrid PS09930066

The parents of hybrid PS09930066 are SLR113-1074 and SBY99-1238. These parents were created as described below.

SLR113-1074 develops large, leafy, upright plants. The plants produce a late-maturing, heavy set of firm, deep, red, mostly three-lobe fruit. The line is resistant to the Tomato spotted wilt virus (Tsw gene; P0). The crossing and selections made to develop this line were as follows:

• • July, Year 1: Collected seed from the Enza Zaden F₁ hybrid LRF4441F.
  • January, Year 2: Planted the F₂ line LRF4441F-M as stake 01LT 3446. Five selections were made.
  • January, Year 3: Planted the F₃ line 01LT 3446-05 as stake 02LT 4625. The line developed a compact plant, with good leaf cover and shortened internodes. The line was resistant to the Tomato spotted wilt virus (Tsw gene; P0). The plants produced a heavy set of firm, half-long, red, smooth fruit. Selections were made.
  • January, Year 4: Planted F₄ inbred 02LT 4625-2 as stake 03LB 03164. Plants grew as a weak plot of big plants and it was questioned if male sterility was being exhibited. No plant selections were made.
  • January, Year 4: Planted F₄ inbred 02LT 4625-2 as stake 03LB LBGH 8512 in the greenhouse. The line grew as leafy, small to medium plants that produced a heavy set of dark red of smooth fruit with variable shape. The inbred line was fixed for resistance to the Tomato spotted wilt virus (Tsw gene; P0). The plants in the plot were massed as 03LB LBGH 8512-M.
  • July, Year 4: Planted F₅ inbred 03LB LBGH 8512-M as stake 03LB 09037. The line produced a heavy set of rather small, firm fruit. There was some question if the line was segregating for set. Selections were made.
  • January, Year 5: Planted F₆ inbred line of 03LB 09037-03 as stake 04LB 03377. The line grew as large plants that produced firm, red, late-maturing small fruit. The line tested fixed for resistance to the Tomato spotted wilt virus (Tsw gene; P0). Plant selections were made.
  • March, Year 6: The F₆ inbred line 04LB 03377-01 was planted as stake 05LT 4374. The line was bulked as 05LT4374-M
  • March, Year 7: 05LT 4374-M was submitted to foundation seed as SLR113-1074.

SBY99-1238 develops a very large anthocyaninless plant that produces a gradual set of large, deep and tapered, ribby, semi-soft fruit. The fruit matures from a medium green to a yellow color. The line is resistant to Tobamo virus (P0), bacterial leaf spot (BS; Bs2 gene, races 0-3, 7, 8; caused by Xanthomonas campestris pv. vesicatoria), Pepper mottle virus (PepMoV), Potato virus Y (PVY 1.2), and is moderately resistant to Phytopthora capsici (PB). The crossing and selections made to develop this line were as follows:

• • January, Year 1: Breeders produced the hybrid SVR 2704405 from parents 99LB 7173-01 and 99LB 7699-01.
  • July, Year 1: Planted the F₁ hybrid SVR 2704405 as stake 00LB 5731. The line was bulked.
  • January, Year 2: Planted the F₂ line 00LB 5731-M as stake 01LB 4372. The line was observed to produce a heavy set of good sized, smooth, medium green maturing to red fruit. Selections were made.
  • January, Year 3: Planted the F₃ line 01LB 4372-05 as stake 02LB 1662. The line developed a medium-large, anthocyaninless plant and was found resistant to PepMoV, PVY 1.2 and susceptible to PB. Selections were made.
  • January, Year 4: Planted F₄ inbred 02LB 1662-02 as stake 03LB 4355. The line was observed to develop a gradual set of smooth firm yellow fruit. Pathology tests indicated moderate resistance to PB and resistance to BS. Selections were made.
  • July, Year 4: Planted F₅ inbred 03LB 4355-01 as stake 03LB 8359. The line was observed to produce leafy, medium-sized, late setting plants that produced a potentially poor set of fruit. Pathology tests indicated moderate resistance to PB. Selections were made.
  • January, Year 5: Planted F₆ inbred 03LB 8359-03 as stake 04LB 2167. The line was observed to develop a weak-branched, medium-sized plant that produced a concentrated, heavy set of large, deep, yellow fruit. Further pathology tests indicated moderate resistance to PB. The line was massed.
  • July, Year 5: Planted F₇ inbred 04LB 2167-M as stake 04LB 07862. The line was observed to develop a very large plant. It produced a gradual set of large, deep and tapered, ribby, semi-soft fruit. The fruit matured from a medium green to yellow color.
  • November, Year 5: 04LB 2167-M was submitted to foundation seed as the finished line, SBY99-1238.

The parent lines are uniform and stable, as is a hybrid therefrom. A small percentage of variants can occur within commercially acceptable limits for almost any characteristic during the course of repeated multiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Pepper Hybrid PS09930066, Pepper Line SLR113-1074 and Pepper Line SBY99-1238

In accordance with one aspect of the present invention, there is provided a plant having the physiological and morphological characteristics of pepper hybrid PS09930066 and the parent lines thereof. A description of the physiological and morphological characteristics of such plants is presented in Tables 1-3.

TABLE 1
Physiological and Morphological Characteristics of Hybrid PS09930066
	CHARACTERISTIC	PS09930066	Cubanelle
1.	Species	C. annuum	C. annuum
2.	Maturity (in region of best adaptability)
	Days from transplanting until mature green	57	60
	stage
	Days from transplanting until mature red or	110	74
	yellow stage
	Days from direct seeding until mature green	83	97
	stage
	Days from direct seeding until mature red or	136	111
	yellow stage
3.	Plant
	Habit	Semi-spreading	Semi-spreading
	Attitude	Upright/erect	Semi-upright/
		(De Cayenne, Doux	semi-erect
		très long des Landes,
		Piquant d'Algérie)
	Plant height	38.1 cm	40.3 cm
	Plant width	36.7 cm	41.15 cm
	Length of stem from cotyledon to first	13.4 cm	18.05 cm
	flower
	Length of the third internode (from soil	77.5 mm	47.25 mm
	surface)
	Length of stem	Long	Long
		(Lipari, Marconi,
		Rouge long ordinaire)
	Shortened internode (in upper part)	Present	Absent
		(Feher, Kalocsai 601,
		Kalocsai 702)
	For varieties with shortened internodes:	More than three
	number of internodes between the first	(Kalocsai 702)
	flower and the shortened internodes
	For varieties without shortened internodes:		Medium
	length of internode (on primary side shoots)
	Stem: hairiness of nodes	Weak	Absent or very
		(Andevalo, Clovis)	weak
	Height	Tall	Medium
		(Century, Orias)
	Basal branches	None	Few
	Branch flexibility	Rigid	Rigid
		(Yolo Wonder)
	Stem strength (breakage resistance)	Weak	Intermediate
	Seedling: anthocyanin coloration of	Weak	Strong
	hypocotyl
	Anthocyanin coloration of stem	Moderate	Absent
	Anthocyanin coloration of nodes	Strong	Moderate
		(California wonder)
	Stem: intensity of anthocyanin coloration of	Strong	Medium
	nodes	(Piquant d'Algérie,
		Zarai)
	Anthocyanin coloration of leaf	Absent	Absent
	Anthocyanin coloration of pedicel	Absent	Absent
	Anthocyanin coloration of calyx	Absent	Absent
	Beginning of flowering (1st flower on 2nd	Early	Early
	flowering node)	(Carré doux extra
		hâtif, Cupido, Fehér,
		Flaviano, Lito,
		Trophy)
	Time of maturity	Medium	Early
		(Lamuyo, Latino,
		Sonar)
4.	Leaf
	Length of blade	Long	Long
		(Cupido, Dolmy,
		Encore, Mazurka,
		Monte)
	Width of blade	Broad	Narrow
		(California wonder,
		Golden calwonder,
		Seifor, Solario)
	Leaf width	47 mm	40.1 mm
	Leaf length	76 mm	82.6 mm
	Petiole length	29.8 mm	38 mm
	Leaf color	Medium green	Yellow-green
	Leaf color (RHS color chart value)	146A	147B
	Intensity of green color	Medium	Medium
		(Doux tres long des
		Landes, Merit)
	Mature leaf shape	Ovate	Lanceolate
		(Balico, Sonar)
	Leaf and stem pubescence	Absent	Absent
	Undulation of margin	Very weak	Absent
	Blistering	Medium	Weak
		(Merit)
	Profile in cross section	Moderately concave	Strongly concave
		(Doux italien,
		Favolor)
	Glossiness	Medium (Alby, Eolo)	Strong
	Peduncle attitude	Semi-drooping	Semi-drooping
		(Blondy)
5.	Flower
	Number of flowers per leaf axil	1	1
	Number of calyx lobes	6	7
	Number of petals	6	6
	Diameter	26.3 mm	26.2 mm
	Corolla color	White	White
	Corolla throat markings	Yellow	Yellow
	Anther color	Purple	Purple
	Style length	Same as stamen	Less than stamen
	Self-incompatability	Absent	Absent
	Anthocyanin coloration in anther	Present	present
		(Lamuyo)
6.	Fruit
	Group	Bell	Cuban (Cubanelle)
		(Yolo Wonder L.)
	Color (Before maturity)	Green	Green
		(California wonder,
		Lamuyo)
	Intensity of color (before maturity)	Medium	Light
	Immature fruit color	Medium green	Light green
	Immature fruit color (RHS color chart	134A	144A
	value)
	Attitude/position	Drooping/pendent	Drooping/pendent
		(De Cayenne,
		Lamuyo)
	Length	Long	Long
		(Doux d'Espagne,
		Majister)
	Diameter	Broad	Narrow
		(Clovis, Lamuyo)
	Ratio length/diameter	Large	Large
		(Heldor, Lamuyo,
		Magister, Tenno,
		Vidi)
	Calyx diameter	30 mm	22.2 mm
	Fruit length	106.6 mm	190.5 mm
	Fruit diameter at calyx attachment	71 mm	54 mm
	Fruit diameter at mid-point	68.6 mm	38.1 mm
	Flesh thickness at mid-point	5.5 mm	9 mm
	Average number of fruits per plant	11	10
	% large fruits	31% (weight range:	17% (weight
		151-190 g)	range: 101-150 g)
	% medium fruits	60% (weight range:	33% (weight
		50-150 g)	range: 51-100 g)
	% small fruits	9% (weight range:	50% (weight
		10-49 g)	range: 1-50 g)
	Measurements (average fruit weight)	101.9 gm	60.8 gm
	Shape in longitudinal section	Trapezoidal	Trapezoidal
		(Dolta, Piperade)
	Shape in cross section (at level of placenta)	Circular	Circular
		(Cherry Sweet, Doux
		très long des Landes)
	Sinuation of pericarp at basal part	Weak	Weak
		(Donat)
	Sinuation of pericarp excluding basal part	Weak	Medium
		(Clovis, Sonar)
	Texture of surface	Smooth or very	Strong wrinkled/
		slightly wrinkled	rough
		(Milord)
	Color (at maturity)	Red	Red
		(Feher Lamuyo)
	Intensity of color (at maturity)	Dark	Medium
	Mature fruit color	Red	Red
	Mature fruit color (RHS color chart value)	N34A	N34A
	Glossiness	Medium/moderate	Medium
		(Carré doux extra
		hâtif, Lamuyo, Sonar)
	Stalk cavity	Present	Absent
		(Bingor, Lamuyo)
	Depth of stalk cavity	Medium
		(Lamuyo, Magister)
	Pedicel length	44.3 mm	32.4 mm
	Pedicel thickness	9.7 mm	9.1 mm
	Pedicel shape	Curved	Curved
	Pedicel cavity	Present	Absent
	Measurements (depth of pedicel cavity)	19.6 mm
	Stalk length	Long	Medium
		(De Cayenne, Sierra
		Nevada, Sweet
		banana)
	Stalk thickness	Medium	Medium
		(Doux italien,
		Surpas)
	Base shape	Cupped	Cupped
	Shape of apex	Moderately depressed	Moderately
		(Quadrato a'Asti	depressed
		rosso)
	Shape	Oblate (Sunnybrook)	Elongate (Long
			Thin Cayenne)
	Fruit set	Concentrated	Concentrated
	Depth of interloculary grooves	Shallow	Shallow
		(Milord, Topgirl)
	Number of locules	Predominantly four	Predominantly
		and more	three
		(Palio, PAZ szentesi)
	Fruits with two locules	0%	14.2%
	Fruits with three locules	30%	57.1%
	Fruits with four locules	63.30%	21.4%
	Fruits with five or more locules	6.70%	7.3%
	Average number of locules	4	3
	Thickness of flesh	Thick	Medium
		(Andevalo, Bingor,
		Daniel, Topgirl)
	Calyx: aspect	Non-enveloping/	Non-enveloping/
		saucer-shaped	saucer-shaped
		(Lamuyo, Sonar)
	Pungency	Sweet	Sweet
	Capsaicin in placenta	Absent	Absent
		(Sonar)
	Flavor	Mild pepper flavor	Mild pepper flavor
	Glossiness	Moderate	Moderate
	Anthocyanin coloration	Absent	Absent
		(Lamuyo)
7.	Seed
	Seed cavity length	96.7 mm	97.6 mm
	Seed cavity diameter	54.2 mm	34.2 mm
	Placenta length	27.5 mm	27.1 mm
	Number of seeds per fruit	138	192
	Grams per 1000 seeds	6.2 gm	3.8 gm
	Seed color	Yellow	Yellow
*These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

TABLE 2
Physiological and Morphological Characteristics of Line SLR113-1074
			Early Cal
	CHARACTERISTIC	SLR113-1074	Wonder
1.	Species	C. annuum	C. annuum
2.	Maturity (in region of best adaptability)
	Number of days from transplanting until	72	67
	mature green stage
	Number of days from transplanting until	138	85
	mature red or yellow stage
	Number of days from direct seeding until	98	104
	mature green stage
	Number of days from direct seeding until	164	122
	mature red or yellow stage
3.	Plant
	Habit	Compact	Compact
	Attitude	Upright/erect	Upright/erect
		(De Cayenne, Doux
		très long des Landes,
		Piquant d'Algérie)
	Plant height	31 cm	40.9 cm
	Plant width	33 cm	47.1 cm
	Length of stem from cotyledon to first	12.9 cm	10.7 cm
	flower
	Length of the third internode (from soil	73.7 mm	54 mm
	surface)
	Length of stem	Long	Medium
		(Lipari, Marconi,
		Rouge long ordinaire)
	Shortened internode (in upper part)	Present	Absent
		(Fehér, Kalocsai 601,
		Kalocsai 702)
	For varieties with shortened internodes:	More than three
	number of internodes between the first	(Kalocsai 702)
	flower and the shortened internodes
	For varieties without shortened internodes:		Medium
	length of internode (on primary side shoots)
	Stem: hairiness of nodes	Absent or very Weak	Absent or very
		(Arlequin)	weak
	Height	Tall	Medium
		(Century, Orias)
	Basal branches	None	Few
	Branch flexibility	Rigid	Rigid
		(Yolo Wonder)
	Stem strength (breakage resistance)	Weak	Intermediate
	Seedling: anthocyanin coloration of	Absent	Moderate
	hypocotyl	(Albaregia, Albena)
	Anthocyanin coloration of stem	Weak	Absent
	Anthocyanin coloration of nodes	Weak	Weak
	Stem: intensity of anthocyanin coloration of	Very weak	Medium
	nodes
	Anthocyanin coloration of leaf	Absent	Absent
	Anthocyanin coloration of pedicel	Absent	Absent
	Anthocyanin coloration of calyx	Absent	Absent
	Beginning of flowering (1st flower on 2nd	Early	Early
	flowering node)	(Carré doux extra
		hâtif, Cupido, Fehér,
		Flaviano, Lito,
		Trophy)
	Time of maturity	Very Late	Medium
		(Cancun, California
		wonder)
4.	Leaf
	Length of blade	Long (Cupido,	Medium
		Dolmy, Encore,
		Mazurka, Monte)
	Width of blade	Broad (California	Medium
		wonder, Golden
		calwonder, Seifor,
		Solario)
	Leaf width	50.4 mm	60 mm
	Leaf length	83.3 mm	113.3 mm
	Petiole length	29.2 mm	46 mm
	Leaf color	Light green	Light green
	Leaf color (RHS color chart value)	146A	147A
	Intensity of green color	Dark	Light
		(Dolmy, Tinto)
	Mature leaf shape	Ovate	Ovate
		(Balico, Sonar)
	Leaf and stem pubescence	Absent	Absent
	Undulation of margin	Weak	Absent
		(Doux très long des
		Landes)
	Blistering	Weak	Weak
		(Pusztagold)
	Profile in cross section	Flat	Moderately
		(De Cayenne, Recio)	concave
	Glossiness	Medium	Medium
		(Alby, Eolo)
	Peduncle attitude	Semi-drooping	Erect
		(Blondy)
5.	Flower
	Number of flowers per leaf axil	1	1
	Number of calyx lobes	6	6
	Number of petals	6	6
	Diameter	27.8 mm	25.1 mm
	Corolla color	White	White
	Corolla throat markings	Yellow	Yellow
	Anther color	Purple	Purple
	Style length	Same as stamen	Same as stamen
	Self-incompatibility	Absent	Absent
	Anthocyanin coloration in anther	Absent	Present
		(Danza)
6.	Fruit
	Group	Bell	Bell
		(Yolo Wonder L.)	(Yolo Wonder L.)
	Color (before maturity)	Green	Green
		(California wonder,
		Lamuyo)
	Intensity of color (before maturity)	Medium	Medium
	Immature fruit color	Medium green	Medium green
	Immature fruit color (RHS color chart	146A	137A
	value)
	Attitude/position	Dropping/pendent	Drooping/pendent
		(De Cayenne,
		Lamuyo)
	Length	Medium	Medium
		(Fehér, Lamuyo)
	Diameter	Medium	Broad
		(Doux italien, Como
		di toro)
	Ratio length/diameter	Medium	Medium
		(Adra, Cherry Sweet,
		Daniel, Delphin,
		Edino)
	Calyx diameter	26.9 mm	32 mm
	Fruit length	89.6 mm	80 mm
	Fruit diameter at calyx attachment	58.7 mm	70 mm
	Fruit diameter at mid-point	53 mm	80 mm
	Flesh thickness at mid-point	4.8 mm	6 mm
	Average number of fruits per plant	5	10
	% large fruits	81% (weight range:	50% (weight range
		50-130 g)	140-199 g)
	% medium fruits	17% (weight range	30% (weight range
		20-49 g)	80-139 g)
	% small fruits	2% (weight range 9-19 g)	20% (weight range
			20-79 g)
	Average fruit weight	83.6 gm	100 gm
	Shape in longitudinal section	Trapezoidal	Square
		(Delta, Piperade)
	Shape in cross section (at level of placenta)	Circular	Quadrangular
		(Cherry Sweet, Doux
		très long des Landes)
	Sinuation of pericarp at basal part	Weak	Very weak
		(Donat)
	Sinuation of pericarp excluding basal part	Weak	Very weak
		(Clovis, Sonar)
	Texture of surface	Slightly wrinkled	Smooth or very
		(Doux très long des	slightly wrinkled
		Landes)
	Color (at maturity)	Red	Red
		(Fehér, Lamuyo)
	Intensity of color (at maturity)	Medium	Dark
	Mature fruit color	Red	Red
	Mature fruit color (RHS color chart value)	44A	46A
	Glossiness	Medium/moderate	Medium/moderate
		(Carré doux extra
		hatif, Lamuyo, Sonar)
	Stalk cavity	Present	Absent
		(Bingor, Lamuyo)
	Depth of stalk cavity	Medium
		(Lamuyo, Magister)
	Pedicel length	39.2 mm	20 mm
	Pedicel thickness	7.9 mm	6 mm
	Pedicel shape	Curved	Curved
	Pedicel cavity	Present	Absent
	Measurements (depth of pedicel cavity)	15.7 mm
	Stalk length	Medium	Medium
		(Fehér, Sonar)
	Stalk thickness	Medium	Medium
		(Doux italien,
		Surpas)
	Base shape	Cupped	Cupped
	Shape of apex	Moderately depressed	Very depressed
		(Quadrato a'Asti
		rosso)
	Shape	Bell	Bell
		(Yolo Wonder L.)	(Yolo Wonder L.)
	Fruit set	Scattered	Scattered
	Depth of interloculary grooves	Medium	Medium
		(Clovis, Lamuyo,
		Marconi)
	Number of Locules	Predominantly three	Predominantly
		(Century)	four and more
	Fruits with two locules	16.70%	0%
	Fruits with three locules	53.30%	40%
	Fruits with four locules	30%	60%
	Average number of locules	3	4
	Thickness of flesh	Medium	Thick
		(Fehér, Lamuyo)
	Calyx aspect	Non-enveloping/	Non-enveloping/
		saucer-shaped	saucer-shaped
		(Lamuyo, Sonar)
	Pungency	Sweet	Sweet
	Capsaicin in placenta	Absent (Sonar)	Absent
	Flavor	Mild pepper flavor	Moderate
	Glossiness	Moderate	Shiny
	Anthocyanin coloration	Absent	Absent
		(Lamuyo)
7.	Seed
	Seed cavity length	77.8 mm	43 mm
	Seed cavity diameter	42.1 mm	52 mm
	Placenta length	20.3 mm	22 mm
	Number of seeds per fruit	41	100
	Grams per 1000 seeds	4.5 gm	7.5 gm
	Seed color	Yellow	Yellow
*These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

TABLE 3
Physiological and Morphological Characteristics of Line SBY99-1238
			Early Cal
	CHARACTERISTIC	SBY99-1238	Wonder
1.	Species	C. annuum	C. annuum
2.	Maturity (in region of best adaptability)
	Number of days from transplanting until	57	67
	mature green stage
	Number of days from transplanting until	127	85
	mature red or yellow stage
	Number of days from direct seeding until	83	104
	mature green stage
	Number of days from direct seeding until	157	122
	mature red or yellow stage
3	Plant
	Habit	Compact	Compact
	Attitude	Upright/erect (De	Upright/erect
		Cayenne, Doux très
		long des Landes,
		Piquant d'Algérie)
	Plant height	31 cm	40.9 cm
	Plant width	34 cm	47.1 cm
	Length of stem from cotyledon to first	12 cm	10.7 cm
	flower
	Length of the third internode (from soil	68 mm	54 mm
	surface)
	Length of stem	Medium (Belsir,	Medium
		Lamuyo)
	Shortened internode (in upper part)	Present (Fehér,	Absent
		Kalocsai 601,
		Kalocsai 702)
	For varieties with shortened internodes:	One to three (Fehér)
	number of internodes between the first
	flower and the shortened internodes
	For varieties without shortened internodes:		Medium
	length of internode (on primary side shoots)
	Stem: hairiness of nodes	Absent or very weak	Absent or very
		(Arlequin)	weak
	Height	Medium (HRF)	Medium
	Basal branches	Few (2-3)	Few
	Branch flexibility	Rigid (Yolo Wonder)	Rigid
	Stem strength (breakage resistance)	Weak	Intermediate
	Seedling: anthocyanin coloration of	Absent (Albaregia,	Moderate
	hypocotyl	Albena)
	Anthocyanin coloration of stem	Moderate	Absent
	Anthocyanin coloration of nodes	Moderate	Weak
	Stem: intensity of anthocyanin coloration of	Medium (Clovis,	Medium
	nodes	Lamuyo, Sonar)
	Anthocyanin coloration of leaf	Absent	Absent
	Anthocyanin coloration of pedicel	Weak	Absent
	Anthocyanin coloration of calyx	Absent	Absent
	Beginning of flowering (1st flower on 2nd	Early (Carré doux	Early
	flowering node)	extra hâtif, Cupido,
		Fehér, Flaviano, Lito,
		Trophy)
	Time of maturity	Very late (Cancun,	Medium
		California wonder)
4.	Leaf
	Length of blade	Long (Cupido,	Medium
		Dolmy, Encore,
		Mazurka, Monte)
	Width of blade	Broad (California	Medium
		wonder, Golden
		calwonder, Seifor,
		Solario)
	Leaf width	53.2 mm	60 mm
	Leaf length	97.8 mm	113.3 mm
	Petiole length	37 mm	46 mm
	Leaf color	Medium green	Light green
	Leaf color (RHS color chart value)	146A	147A
	Intensity of green color	Medium (Doux très	Light
		long des Landes,
		Merit)
	Mature leaf shape	Broad elliptic	Ovate
		(Solario)
	Leaf and stem pubescence	Light	Absent
	Undulation of margin	Weak (Doux très	Absent
		long des Landes)
	Blistering	Weak (Pusztagold)	Weak
	Profile in cross section	Strongly concave	Moderately
		(Slávy)	concave
	Glossiness	Very weak (Diavolo)	Medium
	Peduncle attitude	Semi-drooping	Erect
		(Blondy)
5.	Flower
	Number of flowers per leaf axil	1	1
	Number of calyx lobes	6	6
	Number of petals	6	6
	Diameter	22.4 mm	25.1 mm
	Corolla color	White	White
	Corolla throat markings	Yellow	Yellow
	Anther color	Yellow	Purple
	Style length	Same as stamen	Same as stamen
	Self-incompatability	Absent	Absent
	Anthocyanin coloration in anther	Absent (Danza)	Present
6.	Fruit
	Group	(Bell (Yolo Wonder	(Bell (Yolo
		L.)	Wonder L.)
	Color (before maturity)	Green (California	Green
		wonder, Lamuyo)
	Intensity of color (before maturity)	Medium	Medium
	Immature fruit color	Medium green	Medium green
	Immature fruit color (RHS color chart	144A	137A
	value)
	Attitude/position	Dropping/pendent	Drooping/pendent
		(De Cayenne,
		Lamuyo)
	Length	Medium (Fehér,	Medium
		Lamuyo)
	Diameter	Broad (Clovis,	Broad
		Lamuyo)
	Ratio length/diameter	medium (Adra,	Medium
		Cherry Sweet,
		Daniel, Delphin,
		Edino)
	Calyx diameter	30.4 mm	32 mm
	Fruit length	96.8 mm	80 mm
	Fruit diameter at calyx attachment	69.9 mm	70 mm
	Fruit diameter at mid-point	65.5 mm	80 mm
	Flesh thickness at mid-point	8.2 mm	6 mm
	Average number of fruits per plant	8	10
	% large fruits	46.50% (weight	50% (weight
		range: 150-180 g)	range: 140-199 g)
	% medium fruits	42% (weight range:	30% (weight
		50-149 g)	range: 80-139 g)
	% small fruits	11.50% (weight	20% (weight
		range: 10-49 g)	range: 20-79 g)
	Measurements (average fruit weight)	110.4 gm	100 gm
	Shape in longitudinal section	Trapezoidal (Delta,	Square
		Piperade)
	Shape in cross section (at level of placenta)	Quadrangular	Quadrangular
	Sinuation of pericarp at basal part	Weak (Donat)	Very weak
	Sinuation of pericarp excluding basal part	Weak (Clovis, Sonar)	Very weak
	Texture of surface	Slightly wrinkled	Smooth or very
		(Doux très long des	slightly wrinkled
		Landes)
	Color (at maturity)	Orange (Ariane)	Red
	Intensity of color (at maturity)	Light	Dark
	Mature fruit color	Orange	Red
	Mature fruit color (RHS color chart value)	N30D	46A
	Glossiness	Medium/moderate	Medium/moderate
		(Carré doux extra
		hâtif, Lamuyo, Sonar)
	Stalk cavity	Present (Bingor,	Absent
		Lamuyo)
	Depth of stalk cavity	Very deep (Cancun,
		Cubor, Pablor, Shy
		Beauty)
	Pedicel length	50.7 mm	20 mm
	Pedicel thickness	9 mm	6 mm
	Pedicel shape	Curved	Curved
	Pedicel cavity	Present	Absent
	Measurements (depth of pedicel cavity)	23.2 mm
	Stalk length	Medium (Fehér,	Medium
		Sonar)
	Stalk thickness	Thick (Lamuyo,	Medium
		Trophy, Palio)
	Base shape	Cupped	Cupped
	Shape of apex	Rounded (Cherry	Very depressed
		Sweet)
	Shape	Bell (Yolo Wonder	Bell (Yolo Wonder
		L.)	L.)
	Fruit set	Scattered	Scattered
	Depth of interloculary grooves	Deep (Majister,	Medium
		Surpas)
	Number of Locules	Predominantly four	Predominantly
		and more (Palio, PAZ	four and more
		szentesi)
	Fruits with three locules	23.30%	40%
	Fruits with four locules	60%	60%
	Fruits with five or more locules	16.70%	0%
	Average number of locules	4	4
	Thickness of flesh	Thick (Andevalo,	Thick
		Bingor, Daniel,
		Topgirl)
	Calyx: aspect	Non-	Non-
		enveloping/saucer-	enveloping/saucer-
		shaped (Lamuyo,	shaped
		Sonar)
	Pungency	Sweet	Sweet
	Capsaicin in placenta	Absent (Sonar)	Absent
	Flavor	Mild pepper flavor	Moderate
	Glossiness	Moderate	Shiny
	Anthocyanin coloration	Absent (Lamuyo)	Absent
7.	Seed
	Seed cavity length	107.2 mm	43 mm
	Seed cavity diameter	59.1 mm	52 mm
	Placenta length	19.2 mm	22 mm
	Number of seeds per fruit	111	100
	Grams per 1000 seeds	7 gm	7.5 gm
	Seed color	Yellow	Yellow
*These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

C. Breeding Pepper Plants

One aspect of the current invention concerns methods for producing seed of pepper hybrid PS09930066 involving crossing pepper lines SLR113-1074 and SBY99-1238. Alternatively, in other embodiments of the invention, hybrid PS09930066, line SLR113-1074, or line SBY99-1238 may be crossed with itself or with any second plant. Such methods can be used for propagation of hybrid PS09930066 and/or the pepper lines SLR113-1074 and SBY99-1238, or can be used to produce plants that are derived from hybrid PS09930066 and/or the pepper lines SLR113-1074 and SBY99-1238. Plants derived from hybrid PS09930066 and/or the pepper lines SLR113-1074 and SBY99-1238 may be used, in certain embodiments, for the development of new pepper varieties.

The development of new varieties using one or more starting varieties is well known in the art. In accordance with the invention, novel varieties may be created by crossing hybrid PS09930066 followed by multiple generations of breeding according to such well known methods. New varieties may be created by crossing with any second plant. In selecting such a second plant to cross for the purpose of developing novel lines, it may be desired to choose those plants which either themselves exhibit one or more selected desirable characteristics or which exhibit the desired characteristic(s) when in hybrid combination. Once initial crosses have been made, inbreeding and selection take place to produce new varieties. For development of a uniform line, often five or more generations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way of double-haploids. This technique allows the creation of true breeding lines without the need for multiple generations of selfing and selection. In this manner true breeding lines can be produced in as little as one generation. Haploid embryos may be produced from microspores, pollen, anther cultures, or ovary cultures. The haploid embryos may then be doubled autonomously, or by chemical treatments (e.g. colchicine treatment). Alternatively, haploid embryos may be grown into haploid plants and treated to induce chromosome doubling. In either case, fertile homozygous plants are obtained. In accordance with the invention, any of such techniques may be used in connection with a plant of the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossing transfers a specific desirable trait from one inbred or non-inbred source to an inbred that lacks that trait. This can be accomplished, for example, by first crossing a superior inbred (A) (recurrent parent) to a donor inbred (non-recurrent parent), which carries the appropriate locus or loci for the trait in question. The progeny of this cross are then mated back to the superior recurrent parent (A) followed by selection in the resultant progeny for the desired trait to be transferred from the non-recurrent parent. After five or more backcross generations with selection for the desired trait, the progeny have the characteristic being transferred, but are like the superior parent for most or almost all other loci. The last backcross generation would be selfed to give pure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for the development of new lines based on the elite nature of the genetic background of the plants. In selecting a second plant to cross with PS09930066 and/or pepper lines SLR113-1074 and SBY99-1238 for the purpose of developing novel pepper lines, it will typically be preferred to choose those plants which either themselves exhibit one or more selected desirable characteristics or which exhibit the desired characteristic(s) when in hybrid combination. Examples of desirable traits may include, in specific embodiments, high seed yield, high seed germination, seedling vigor, high fruit yield, disease tolerance or resistance, and adaptability for soil and climate conditions. Consumer-driven traits, such as a fruit shape, color, texture, and taste are other examples of traits that may be incorporated into new lines of pepper plants developed by this invention.

D. Performance Characteristics

As described above, pepper hybrid PS09930066 and pepper lines SLR113-1074 and SBY99-1238 exhibit desirable agronomic traits. The performance characteristics of hybrid PS09930066 were the subject of an objective analysis of the performance traits relative to other varieties. The results of the analysis are presented below.

TABLE 4
Performance Characteristics For Hybrid PS09930066
			Red
Variety	PS09930066	Maccabi	Lion
Bacterial leaf spot resistance races	yes	no	no
1, 2, 3, 7, 8 caused by Xanthomonas
campestris pv. vesicatoria
Tobamo virus (P0) resistance	yes	no	no
Tomato spotted wilt virus (P0)	yes	no	no
resistance
Potato virus Y (PVY 1.2) resistance	yes	no	no
Pepper mottle virus resistance	yes	no	no
Fruit size	large	medium	large
Fruit wall thickness	thick	medium	medium
Maturity	late	medium	early

TABLE 5
Performance Characteristics for Pepper Line SLR113-1074
		Color	Susceptibility to	Susceptibility
		(RHS	Tomato spotted	to Tobamo
	Shape	value)	wilt virus (P0)	virus (P0)
SLR113-	trapezoidal	light green	resistant	resistant
1074		(146A)
Early Cal	square	green	susceptible	susceptible
Wonder		(137A)

TABLE 6
Performance Characteristics for Pepper Line SBY99-1238
				Susceptibility to
			Susceptibility to	bacterial leaf spot (BS;
			Pepper mottle virus	Bs2 gene, races 0-3, 7, 8)
			(PepMoV) and	caused by Xanthomonas	Susceptibility to	Susceptibility to
		Fruit	Potato virus Y	campestris pv.	Tobamo virus	Phytopthora
	Anthocyanin	Color	(PVY 1.2)	vesicatoria)	(P0)	capsici
SBY99-	Anthers: yellow	yellow	resistant	resistant	resistant	resistant
1238	(absent)
	Fruit: absent
Early Cal	Anthers: slightly	red	susceptible	susceptible	susceptible	susceptible
Wonder	purple (present)
	Fruit: moderate

E. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein are provided modified to include at least a first desired heritable trait. Such plants may, in one embodiment, be developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a variety are recovered in addition to a genetic locus transferred into the plant via the backcrossing technique. The term single locus converted plant as used herein refers to those pepper plants which are developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a variety are recovered in addition to the single locus transferred into the variety via the backcrossing technique. By essentially all of the morphological and physiological characteristics, it is meant that the characteristics of a plant are recovered that are otherwise present when compared in the same environment, other than an occasional variant trait that might arise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improve or introduce a characteristic into the present variety. The parental pepper plant which contributes the locus for the desired characteristic is termed the nonrecurrent or donor parent. This terminology refers to the fact that the nonrecurrent parent is used one time in the backcross protocol and therefore does not recur. The parental pepper plant to which the locus or loci from the nonrecurrent parent are transferred is known as the recurrent parent as it is used for several rounds in the backcrossing protocol.

In a typical backcross protocol, the original variety of interest (recurrent parent) is crossed to a second variety (nonrecurrent parent) that carries the single locus of interest to be transferred. The resulting progeny from this cross are then crossed again to the recurrent parent and the process is repeated until a pepper plant is obtained wherein essentially all of the morphological and physiological characteristics of the recurrent parent are recovered in the converted plant, in addition to the single transferred locus from the nonrecurrent parent.

The selection of a suitable recurrent parent is an important step for a successful backcrossing procedure. The goal of a backcross protocol is to alter or substitute a single trait or characteristic in the original variety. To accomplish this, a single locus of the recurrent variety is modified or substituted with the desired locus from the nonrecurrent parent, while retaining essentially all of the rest of the desired genetic, and therefore the desired physiological and morphological constitution of the original variety. The choice of the particular nonrecurrent parent will depend on the purpose of the backcross; one of the major purposes is to add some commercially desirable trait to the plant. The exact backcrossing protocol will depend on the characteristic or trait being altered and the genetic distance between the recurrent and nonrecurrent parents. Although backcrossing methods are simplified when the characteristic being transferred is a dominant allele, a recessive allele, or an additive allele (between recessive and dominant), may also be transferred. In this instance it may be necessary to introduce a test of the progeny to determine if the desired characteristic has been successfully transferred.

In one embodiment, progeny pepper plants of a backcross in which a plant described herein is the recurrent parent comprise (i) the desired trait from the non-recurrent parent and (ii) all of the physiological and morphological characteristics of pepper the recurrent parent as determined at the 5% significance level when grown in the same environmental conditions.

New varieties can also be developed from more than two parents. The technique, known as modified backcrossing, uses different recurrent parents during the backcrossing. Modified backcrossing may be used to replace the original recurrent parent with a variety having certain more desirable characteristics or multiple parents may be used to obtain different desirable characteristics from each.

Many single locus traits have been identified that are not regularly selected for in the development of a new inbred but that can be improved by backcrossing techniques. Single locus traits may or may not be transgenic; examples of these traits include, but are not limited to, herbicide resistance, resistance to bacterial, fungal, or viral disease, insect resistance, modified fatty acid or carbohydrate metabolism, and altered nutritional quality. These comprise genes generally inherited through the nucleus.

Direct selection may be applied where the single locus acts as a dominant trait. For this selection process, the progeny of the initial cross are assayed for viral resistance and/or the presence of the corresponding gene prior to the backcrossing. Selection eliminates any plants that do not have the desired gene and resistance trait, and only those plants that have the trait are used in the subsequent backcross. This process is then repeated for all additional backcross generations.

Selection of pepper plants for breeding is not necessarily dependent on the phenotype of a plant and instead can be based on genetic investigations. For example, one can utilize a suitable genetic marker which is closely genetically linked to a trait of interest. One of these markers can be used to identify the presence or absence of a trait in the offspring of a particular cross, and can be used in selection of progeny for continued breeding. This technique is commonly referred to as marker assisted selection. Any other type of genetic marker or other assay which is able to identify the relative presence or absence of a trait of interest in a plant can also be useful for breeding purposes. Procedures for marker assisted selection are well known in the art. Such methods will be of particular utility in the case of recessive traits and variable phenotypes, or where conventional assays may be more expensive, time consuming or otherwise disadvantageous. Types of genetic markers which could be used in accordance with the invention include, but are not necessarily limited to, Simple Sequence Length Polymorphisms (SSLPs) (Williams et al., 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858, specifically incorporated herein by reference in its entirety), and Single Nucleotide Polymorphisms (SNPs) (Wang et al., 1998).

F. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well as directly into a plant, are those which are introduced by genetic transformation techniques. Genetic transformation may therefore be used to insert a selected transgene into a plant of the invention or may, alternatively, be used for the preparation of transgenes which can be introduced by backcrossing. Methods for the transformation of plants that are well known to those of skill in the art and applicable to many crop species include, but are not limited to, electroporation, microprojectile bombardment, Agrobacterium-mediated transformation and direct DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ either friable tissues, such as a suspension culture of cells or embryogenic callus or alternatively one may transform immature embryos or other organized tissue directly. In this technique, one would partially degrade the cell walls of the chosen cells by exposing them to pectin-degrading enzymes (pectolyases) or mechanically wound tissues in a controlled manner.

An efficient method for delivering transforming DNA segments to plant cells is microprojectile bombardment. In this method, particles are coated with nucleic acids and delivered into cells by a propelling force. Exemplary particles include those comprised of tungsten, platinum, and preferably, gold. For the bombardment, cells in suspension are concentrated on filters or solid culture medium. Alternatively, immature embryos or other target cells may be arranged on solid culture medium. The cells to be bombarded are positioned at an appropriate distance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plant cells by acceleration is the Biolistics Particle Delivery System, which can be used to propel particles coated with DNA or cells through a screen, such as a stainless steel or Nytex screen, onto a surface covered with target cells. The screen disperses the particles so that they are not delivered to the recipient cells in large aggregates. Microprojectile bombardment techniques are widely applicable, and may be used to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system for introducing gene loci into plant cells. An advantage of the technique is that DNA can be introduced into whole plant tissues, thereby bypassing the need for regeneration of an intact plant from a protoplast. Modern Agrobacterium transformation vectors are capable of replication in E. coli as well as Agrobacterium, allowing for convenient manipulations (Klee et al., 1985). Moreover, recent technological advances in vectors for Agrobacterium-mediated gene transfer have improved the arrangement of genes and restriction sites in the vectors to facilitate the construction of vectors capable of expressing various polypeptide coding genes. The vectors described have convenient multi-linker regions flanked by a promoter and a polyadenylation site for direct expression of inserted polypeptide coding genes. Additionally, Agrobacterium containing both armed and disarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation is efficient, it is the method of choice because of the facile and defined nature of the gene locus transfer. The use of Agrobacterium-mediated plant integrating vectors to introduce DNA into plant cells is well known in the art (Fraley et al., 1985; U.S. Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methods based on calcium phosphate precipitation, polyethylene glycol treatment, electroporation, and combinations of these treatments (see, e.g., Potrykus et al., 1985; Omirulleh et al., 1993; Fromm et al., 1986; Uchimiya et al., 1986; Marcotte et al., 1988). Transformation of plants and expression of foreign genetic elements is exemplified in Choi et al. (1994), and Ellul et al. (2003).

A number of promoters have utility for plant gene expression for any gene of interest including but not limited to selectable markers, scoreable markers, genes for pest tolerance, disease resistance, nutritional enhancements and any other gene of agronomic interest. Examples of constitutive promoters useful for plant gene expression include, but are not limited to, the cauliflower mosaic virus (CaMV) P-35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odel et al., 1985), including in monocots (see, e.g., Dekeyser et al., 1990; Terada and Shimamoto, 1990); a tandemly duplicated version of the CaMV 35S promoter, the enhanced 35S promoter (P-e35S); the nopaline synthase promoter (An et al., 1988); the octopine synthase promoter (Fromm et al., 1989); and the figwort mosaic virus (P-FMV) promoter as described in U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter (P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem; the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform virus promoter; a commelina yellow mottle virus promoter; and other plant DNA virus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response to environmental, hormonal, chemical, and/or developmental signals can also be used for expression of an operably linked gene in plant cells, including promoters regulated by (1) heat (Callis et al., 1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., 1989; maize rbcS promoter, Schaffner and Sheen, 1991; or chlorophyll a/b-binding protein promoter, Simpson et al., 1985), (3) hormones, such as abscisic acid (Marcotte et al., 1989), (4) wounding (e.g., wunl, Siebertz et al., 1989); or (5) chemicals such as methyl jasmonate, salicylic acid, or Safener. It may also be advantageous to employ organ-specific promoters (e.g., Roshal et al., 1987; Schernthaner et al., 1988; Bustos et al., 1989).

Exemplary nucleic acids which may be introduced to plants of this invention include, for example, DNA sequences or genes from another species, or even genes or sequences which originate with or are present in the same species, but are incorporated into recipient cells by genetic engineering methods rather than classical reproduction or breeding techniques. However, the term “exogenous” is also intended to refer to genes that are not normally present in the cell being transformed, or perhaps simply not present in the form, structure, etc., as found in the transforming DNA segment or gene, or genes which are normally present and that one desires to express in a manner that differs from the natural expression pattern, e.g., to over-express. Thus, the term “exogenous” gene or DNA is intended to refer to any gene or DNA segment that is introduced into a recipient cell, regardless of whether a similar gene may already be present in such a cell. The type of DNA included in the exogenous DNA can include DNA which is already present in the plant cell, DNA from another plant, DNA from a different organism, or a DNA generated externally, such as a DNA sequence containing an antisense message of a gene, or a DNA sequence encoding a synthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and could potentially be introduced into a pepper plant according to the invention. Non-limiting examples of particular genes and corresponding phenotypes one may choose to introduce into a pepper plant include one or more genes for insect tolerance, such as a Bacillus thuringiensis (B.t.) gene, pest tolerance such as genes for fungal disease control, herbicide tolerance such as genes conferring glyphosate tolerance, and genes for quality improvements such as yield, nutritional enhancements, environmental or stress tolerances, or any desirable changes in plant physiology, growth, development, morphology or plant product(s). For example, structural genes would include any gene that confers insect tolerance including but not limited to a Bacillus insect control protein gene as described in WO 99/31248, herein incorporated by reference in its entirety, U.S. Pat. No. 5,689,052, herein incorporated by reference in its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, herein incorporated by reference in their entirety. In another embodiment, the structural gene can confer tolerance to the herbicide glyphosate as conferred by genes including, but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat. No. 5,633,435, herein incorporated by reference in its entirety, or glyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No. 5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes by encoding a non-translatable RNA molecule that causes the targeted inhibition of expression of an endogenous gene, for example via antisense- or cosuppression-mediated mechanisms (see, for example, Bird et al., 1991). The RNA could also be a catalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desired endogenous mRNA product (see for example, Gibson and Shillito, 1997). Thus, any gene which produces a protein or mRNA which expresses a phenotype or morphology change of interest is useful for the practice of the present invention.

G. Definitions

In the description and tables herein, a number of terms are used. In order to provide a clear and consistent understanding of the specification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all of which alleles relate to one trait or characteristic. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybrid progeny, for example a first generation hybrid (F₁), back to one of the parents of the hybrid progeny. Backcrossing can be used to introduce one or more single locus conversions from one genetic background into another.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes from different plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation of the organs with a cytoplasmic or nuclear genetic factor or a chemical agent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions. F₁ Hybrid: The first generation progeny of the cross of two nonisogenic plants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets of chromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend to segregate together more often than expected by chance if their transmission was independent.

Marker: A readily detectable phenotype, preferably inherited in codominant fashion (both alleles at a locus in a diploid heterozygote are readily detectable), with no environmental variance component, i.e., heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, which characteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer to genetic loci that control to some degree numerically representable traits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” are used interchangeably to describe plants that show no symptoms to a specified biotic pest, pathogen, abiotic influence or environmental condition. These terms are also used to describe plants showing some symptoms but that are still able to produce marketable product with an acceptable yield. Some plants that are referred to as resistant or tolerant are only so in the sense that they may still produce a crop, even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chart is a standardized reference which allows accurate identification of any color. A color's designation on the chart describes its hue, brightness and saturation. A color is precisely named by the RHS color chart by identifying the group name, sheet number and letter, e.g., Yellow-Orange Group 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigma of the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a pepper variety are recovered in addition to the characteristics of the single locus transferred into the variety via the backcrossing technique and/or by genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does not show a statistically significant difference (e.g., p=0.05) from the mean.

Tissue Culture: A composition comprising isolated cells of the same or a different type or a collection of such cells organized into parts of a plant.

Transgene: A genetic locus comprising a sequence which has been introduced into the genome of a pepper plant by transformation.

H. Deposit Information

A deposit of pepper hybrid PS09930066 and inbred parent lines SLR113-1074 and SBY99-1238, disclosed above and recited in the claims, has been made with the American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va. 20110-2209. The date of the deposits was Jan. 26, 2010. The accession numbers for those deposited seeds of pepper hybrid PS09930066 and inbred parent lines SLR113-1074 and SBY99-1238 are ATCC Accession No. PTA-10613, ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively. Upon issuance of a patent, all restrictions upon the deposits will be removed, and the deposits are intended to meet all of the requirements of 37 C.F.R. §1.801-1.809. The deposits will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if necessary during that period.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the invention, as limited only by the scope of the appended claims.

All references cited herein are hereby expressly incorporated herein by reference.

REFERENCES

The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference:

• U.S. Pat. No. 5,378,619
• U.S. Pat. No. 5,463,175
• U.S. Pat. No. 5,500,365
• U.S. Pat. No. 5,563,055
• U.S. Pat. No. 5,633,435
• U.S. Pat. No. 5,689,052
• U.S. Pat. No. 5,880,275
• An et al., Plant Physiol., 88:547, 1988.
• Bird et al., Biotech. Gen. Engin. Rev., 9:207, 1991.
• Bustos et al., Plant Cell, 1:839, 1989.
• Callis et al., Plant Physiol., 88:965, 1988.
• Choi et al., Plant Cell Rep., 13: 344-348, 1994.
• Dekeyser et al., Plant Cell, 2:591, 1990.
• Ellul et al., Theor. Appl. Genet., 107:462-469, 2003.
• EP 534 858
• Fraley et al., Bio/Technology, 3:629-635, 1985.
• Fromm et al., Nature, 312:791-793, 1986.
• Fromm et al., Plant Cell, 1:977, 1989.
• Gibson and Shillito, Mol. Biotech., 7:125, 1997
• Klee et al., Bio-Technology, 3(7):637-642, 1985.
• Kuhlemeier et al., Plant Cell, 1:471, 1989.
• Marcotte et al., Nature, 335:454, 1988.
• Marcotte et al., Plant Cell, 1:969, 1989.
• Odel et al., Nature, 313:810, 1985.
• Omirulleh et al., Plant Mol. Biol., 21(3):415-428, 1993.
• Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985.
• Roshal et al., EMBO J., 6:1155, 1987.
• Schaffner and Sheen, Plant Cell, 3:997, 1991.
• Schernthaner et al., EMBO J., 7:1249, 1988.
• Siebertz et al., Plant Cell, 1:961, 1989.
• Simpson et al., EMBO J., 4:2723, 1985.
• Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990.
• Uchimiya et al., Mol. Gen. Genet., 204:204, 1986.
• Wang et al., Science, 280:1077-1082, 1998.
• Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990.
• WO 99/31248

Claims

1. A pepper plant comprising at least a first set of the chromosomes of pepper line SLR113-1074 or pepper line SBY99-1238, a sample of seed of said lines having been deposited under ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively.

2. A seed comprising at least a first set of the chromosomes of pepper line SLR113-1074 or pepper line SBY99-1238, a sample of seed of said lines having been deposited under ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively.

3. The plant of claim 1, which is inbred.

4. The plant of claim 1, which is hybrid.

5. The plant of claim 4, wherein the hybrid plant is pepper hybrid PS09930066, a sample of seed of said hybrid PS09930066 having been deposited under ATCC Accession No. PTA-10613.

6. The plant of claim 1, wherein the plant is a plant of line SLR113-1074 or line SBY99-1238.

7. A plant part of the plant of claim 1.

8. The plant part of claim 7, further defined as a leaf, a ovule, pollen, a fruit, or a cell.

9. A pepper plant having all the physiological and morphological characteristics of the pepper plant of claim 5.

10. A pepper plant having all the physiological and morphological characteristics of the pepper plant of claim 6.

11. A tissue culture of regenerable cells of the plant of claim 1.

12. The tissue culture according to claim 11, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.

13. A pepper plant regenerated from the tissue culture of claim 12.

14. A method of vegetatively propagating the plant of claim 1 comprising the steps of:

(a) collecting tissue capable of being propagated from a plant according to claim 1;

(b) cultivating said tissue to obtain proliferated shoots; and

(c) rooting said proliferated shoots to obtain rooted plantlets.

15. The method of claim 14, further comprising growing at least a first plant from said rooted plantlets.

16. A method of introducing a desired trait into a pepper line comprising:

(a) crossing a plant of line SLR113-1074 or SBY99-1238 with a second pepper plant that comprises a desired trait to produce F1 progeny, a sample of seed of said lines having been deposited under ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively;

(b) selecting an F1 progeny that comprises the desired trait;

(c) backcrossing the selected F1 progeny with a plant of line SLR113-1074 or SBY99-1238 to produce backcross progeny;

(d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of pepper line SLR113-1074 or SBY99-1238; and

(e) repeating steps (c) and (d) three or more times to produce selected fourth or higher backcross progeny that comprise the desired trait.

17. A pepper plant produced by the method of claim 16.

18. A method of producing a plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of line PS09930066, line SLR113-1074 or line SBY99-1238, a sample of seed of said hybrid and lines having been deposited under ATCC Accession No. PTA-10613, ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively.

19. A plant produced by the method of claim 18.

20. A method of determining the genotype of the plant of claim 1 comprising obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms.

21. The method of claim 20, further comprising the step of storing the results of detecting the plurality of polymorphisms on a computer readable medium.

22. The plant of claim 1, comprising a transgene.

23. The plant of claim 22, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

24. The plant of claim 1, comprising a single locus conversion.

25. The plant of claim 24, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

26. A method for producing a seed of a plant derived from at least one of hybrid PS09930066, line SLR113-1074 or line SBY99-1238 comprising the steps of:

(a) crossing a pepper plant of hybrid line PS09930066, line SLR113-1074 or line SBY99-1238 with itself or a second pepper plant; a sample of seed of said hybrid and lines having been deposited under ATCC Accession No. PTA-10613, ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively; and

(b) allowing seed of a hybrid PS09930066, line SLR113-1074 or line SBY99-1238-derived pepper plant to form.

27. The method of claim 26, further comprising the steps of:

(c) selfing a plant grown from said hybrid PS09930066, SLR113-1074 or SBY99-1238-derived pepper seed to yield additional hybrid PS09930066, line SLR113-1074 or line SBY99-1238-derived pepper seed;

(d) growing said additional hybrid PS09930066, line SLR113-1074 or line SBY99-1238-derived pepper seed of step (c) to yield additional hybrid PS09930066, line SLR113-1074 or line SBY99-1238-derived pepper plants; and

(e) repeating the crossing and growing steps of (c) and (d) to generate at least a first further hybrid PS09930066, line SLR113-1074 or line SBY99-1238-derived pepper plant.

28. The method of claim 26, wherein the second pepper plant is of an inbred pepper line.

29. The method of claim 26, comprising crossing line SLR113-1074 with line SBY99-1238, a sample of seed of said lines having been deposited under ATCC Accession No. PTA-10617, and ATCC Accession No. PTA-10616, respectively.

30. The method of claim 27, further comprising:

(f) crossing the further hybrid PS09930066, SLR113-1074 or SBY99-1238-derived pepper plant with a second pepper plant to produce seed of a hybrid progeny plant.

31. A hybrid seed produced by the method of claim 29.

32. A plant produced by growing the seed of claim 29.

33. A plant part of the plant of claim 32.

34. The plant part of claim 33, further defined as a leaf, a flower, a fruit, an ovule, pollen, or a cell.

35. A method of producing a pepper seed comprising crossing the plant of claim 1 with itself or a second pepper plant and allowing seed to form.

36. A method of producing a pepper fruit comprising:

(a) obtaining a plant according to claim 1, wherein the plant has been cultivated to maturity; and

(b) collecting a pepper from the plant.