Nucleic Acid Based Assay Involving A Hybridization Step With A Nucleic Acid Probe, Involving A Single Nucleotide Polymorphism (snp), Involving Pharmacogenetics, Involving Genotyping, Involving Haplotyping, Or Involving Detection Of Dna Methylation Gene Expression Patents (Class 435/6.11)
  • Patent number: 10689685
    Abstract: The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting human papillomaviruses, human beta globin sequences and human papillomaviruses and human beta globin sequences in a test sample.
    Type: Grant
    Filed: October 25, 2017
    Date of Patent: June 23, 2020
    Assignee: ABBOTT MOLECULAR INC.
    Inventors: Klara Abravaya, Brian J. Erickson, Shihai X. Huang, Wai-Bing X. Mak, John A. Salituro, Ning Tang
  • Patent number: 10689687
    Abstract: Methods of assaying cells of a cellular sample for the presence of a target nucleic acid are provided. Aspects of the methods include evaluating a cellular sample that has been contacted with a nuclease inhibitor for the presence of a target nucleic acid. Also provided are devices and kits that find use in practicing the methods described herein.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: June 23, 2020
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Garry P. Nolan, Yury Goltsev, Quan Nguyen, Yunqing Ma, Chunfai Lai
  • Patent number: 10689694
    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: June 23, 2020
    Assignee: Life Technologies Corporation
    Inventors: Caifu Chen, Ruoying Tan
  • Patent number: 10683557
    Abstract: It provides a method for predicting the risk of an adverse pregnancy or neonatal outcome for a pregnant subject by detecting the elevated level of bacteria from one or more selected bacterial taxa (e.g., genera or species). A kit useful for such a method is also provided. In addition, it provides a method for determining the risk of having advanced cervical dilation and/or premature cervical shortening based on differentially abundant bacterial taxa.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: June 16, 2020
    Assignee: The Chinese University of Hong Kong
    Inventors: Stephen Siu-Chung Chim, Chee-Yin Cheung, Wan-Chee Cheung, Meng Meng, Tak-Yeung Leung, Keun-Young Lee
  • Patent number: 10683545
    Abstract: The invention relates to the identification of microRNAs associated with acne and to the use thereof.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: June 16, 2020
    Assignee: GALDERMA RESEARCH & DEVELOPMENT
    Inventors: Carine Mounier, Sophie Deret
  • Patent number: 10684301
    Abstract: The invention relates to a sample processing system and method for processing biological samples, comprising a sample processing device having: a receiving plate, which is arranged substantially horizontally in a plane; a first and second working arm, which can move relative to the receiving plate and extend substantially parallel to each other in a second direction (Y) over the receiving plate; at least one pipetting device mounted on the first working arm, which is movable in the second direction (Y) and in a third direction (Z) orthogonal in relation to the first and second direction (X, Y); at least one gripping device, mounted on the second working arm, with grippers that can be rotated around a gripper axis of rotation (GA) parallel to the third direction (Z); and a control device for controlling the pipetting device and the gripping device.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: June 16, 2020
    Assignee: Hamilton Bonaduz AG
    Inventor: Nando Giovanoli
  • Patent number: 10683534
    Abstract: Ligation assays in liquid phase for detecting nucleic acid sequences.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: June 16, 2020
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Anthony Stevens, Bruce Seligmann, Joanne M. Yeakley, Joel McComb
  • Patent number: 10683550
    Abstract: The present disclosure provides methods of detecting a fusion gene of HNF4G and RSPO2 in a nucleic acid-containing sample, and a primer set, a probe set and a kit for detecting the fusion gene are also provided. Animal models for a human disease positive for the fusion gene are also provided herein. In addition, the present disclosure relates to the methods for assessing and identifying an agent effective on the fusion gene of HNF4G and RSPO2 or a human disease positive for a fusion gene of HNF4G and RSPO2 and thereby treating said disease are also provided.
    Type: Grant
    Filed: April 3, 2015
    Date of Patent: June 16, 2020
    Assignee: CROWN BIOSCIENCE, INC. (TAICANG)
    Inventors: Jie Cai, Henry Li
  • Patent number: 10683555
    Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as pancreatic and colorectal cancer.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: June 16, 2020
    Assignees: Mayo Foundation for Medical Education and Research, Exact Sciences Development Company, LLC
    Inventors: David A. Ahlquist, John B. Kisiel, William R. Taylor, Tracy C. Yab, Douglas W. Mahoney, Graham P. Lidgard, Hatim T. Allawi
  • Patent number: 10684199
    Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: June 16, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Kyle Schleifer, Kristin Briana Bernick, Adrienne Mccampbell, Nicholas M Sampas, Victor Lim
  • Patent number: 10676739
    Abstract: The present invention relates to oligonucleotide inhibitors of the TSC22D4 activity or expression and their uses for the prevention, treatment, and/or regulation of insulin resistance, metabolic syndrome and/or diabetes and/or for improving insulin sensitivity in a mammal.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: June 9, 2020
    Assignee: DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS
    Inventors: Stephan Herzig, Mauricio Berriel Diaz, Tobias Schafmeier
  • Patent number: 10679729
    Abstract: Novel haplotype cluster Markov models are used to phase genomic samples. After the models are built, they rapidly and accurately phase new samples without requiring that the new samples be used to re-build the models. The models set transition probabilities such that the probability for an appearance of any allele within any haplotype is a non-zero number. Furthermore, the most unlikely pairs of haplotypes are discarded from each model at each level until c of the likelihood mass at each level is discarded. The models are also constructed such that contributing windows of SNPs partially overlap so that phasing decisions near one of the extreme ends of any model is are not significantly determinative of the phase. Additionally, the models are configured such that two or more nodes can be merged during the building/updating procedure to consolidate haplotype clusters having similar distributions.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: June 9, 2020
    Assignee: Ancestry.com DNA, LLC
    Inventors: Catherine Ann Ball, Keith D. Noto, Kenneth G. Chahine, Mathew J. Barber, Yong Wang
  • Patent number: 10676667
    Abstract: To provide a quantum dot and manufacturing method of the dot particularly capable of reducing organic residues adhering to the quantum dot surface and of suppressing the black discoloration occurrence of a layer including the quantum dot positioned immediately above a light emitting device, and a compact, sheet member, wavelength conversion member and light emitting apparatus with high luminous efficiency using the quantum dot, a quantum dot of the present invention has a core portion including a semiconductor particle, and a shell portion with which the surface of the core portion is coated, and is characterized in that a weight reduction up to 490° C. is within 75% in a TG-DTA profile. Further, the quantum dot of the invention is characterized in that oleylamine (OLA) is not observed in GC-MS qualitative analysis at 350° C.
    Type: Grant
    Filed: September 6, 2017
    Date of Patent: June 9, 2020
    Assignee: NS MATERIALS INC.
    Inventors: Akiharu Miyanaga, Eiichi Kanaumi, Yoshikazu Nageno
  • Patent number: 10668068
    Abstract: Embodiments of the invention find application in the field of cancer therapy. Receptor protein kinases (RPTKs) transmit extracellular signals across the plasma membrane to cytosolic proteins, stimulating formation of complexes that regulate key cellular functions. Over half of the known tyrosine kinases are implicated in human cancers and are therefore highly promising drug targets.
    Type: Grant
    Filed: October 7, 2015
    Date of Patent: June 2, 2020
    Assignee: The Research Foundation For The State University Of New York
    Inventors: Xianhui Wang, Douglas S. Conklin
  • Patent number: 10669585
    Abstract: Disclosed is a method to achieve digital quantification of DNA (i.e., counting differences between identical sequences) using direct shotgun sequencing followed by mapping to the chromosome of origin and enumeration of fragments per chromosome. The preferred method uses massively parallel sequencing, which can produce tens of millions of short sequence tags in a single run and enabling a sampling that can be statistically evaluated. By counting the number of sequence tags mapped to a predefined window in each chromosome, the over- or under-representation of any chromosome in maternal plasma DNA contributed by an aneuploid fetus can be detected. This method does not require the differentiation of fetal versus maternal DNA. The median count of autosomal values is used as a normalization constant to account for differences in total number of sequence tags is used for comparison between samples and between chromosomes.
    Type: Grant
    Filed: June 28, 2016
    Date of Patent: June 2, 2020
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Hei-Mun Christina Fan, Stephen R. Quake
  • Patent number: 10670526
    Abstract: An improved DNA sequencing system comprising a DNA sample holder residing on a stacked BSI global shutter image sensor illuminated by a pulsed laser for fluorescent illumination detection. The pulsed laser has on and off periods wherein during the laser on period a Fluorophore tag attached to a DNA sample is excited to produce fluorescence emission while the imaging system captures no illumination and during the off period the global shutter imaging system captures persistent fluorescent emission from the DNA sample and reads out an imaging signal.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: June 2, 2020
    Assignee: SmartSens Technology (cayman) Co., Limited
    Inventors: Chen Xu, Yaowu Mo, Zexu Shao, Zhengmin Zhang, Weijian Ma
  • Patent number: 10669571
    Abstract: Provided herein are methods and compositions for depleting targeted nucleic acid sequences from a sample, enriching for sequences of interest from a sample, and/or partitioning of sequences from a sample. The methods and compositions are applicable to biological, clinical, forensic, and environmental samples.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 2, 2020
    Assignee: Arc Bio, LLC
    Inventors: Meredith L. Carpenter, Carlos D. Bustamante, Stephane B. Gourguechon
  • Patent number: 10670591
    Abstract: The invention, depending on aspect and embodiment, relates to capture probe controls, and capture and signal probe configurations and combinations of configurations that can facilitate accurate and efficient multiplex analyte detection, especially in electrochemical detection schemes.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: June 2, 2020
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Michael Leonard Wood, Aviva Ruth Jacobs
  • Patent number: 10665325
    Abstract: A method, computer program product, and system for identifying a surface area size of a biosensing structure, for use in a bionsensor device, based on a plurality of nucleotides structures under test. A first set of properties are determined comprising: reaction coordinate values, and potential of mean force (PMF) values, for the plurality of nucleotide structures based on a first set of testing conditions comprising a first surface area material, a first surface area pattern, and a first surface area size. A second set of properties is determined comprising reaction coordinate values, and PMF values, for the plurality of nucleotide structures based on a second set of testing conditions comprising a second surface area material, a second surface area pattern, a second surface area size, or a combination thereof and a target population of nucleotide structures among the plurality of nucleotide structures are identified.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: May 26, 2020
    Assignee: International Business Machines Corporation
    Inventors: Robert L. Bruce, Payel Das, HsinYu Tsai, Sufi Zafar
  • Patent number: 10654038
    Abstract: A nucleic acid analysis apparatus with isothermal based amplification includes a chamber, a fluid delivery unit, a thermal unit, a rotational driven unit and at least one optical unit. The chamber includes a cartridge mounted therein. The fluid delivery unit is connected with the chamber and adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction. The thermal unit is disposed in the chamber and adapted to provide a predefined temperature for nucleic acid amplification. The rotational driven unit is connected with the chamber and capable of rotating the cartridge with a predefined program. The at least one optical unit is disposed on the chamber and includes plural optical components for detection.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: May 19, 2020
    Assignee: DELTA ELECTRONICS INT'L (SINGAPORE) PTE LTD
    Inventors: Qian Liang, Bo Ma, Shuwen An, Weishi Zhang
  • Patent number: 10655179
    Abstract: The present invention provides novel mutations of the CFTR gene related to cystic fibrosis or to conditions associated with cystic fibrosis. The mutations include duplication of exons including duplication of exons 6b through 10. Methods of identifying if an individual contains the exons 6b through 10 duplication are provided as well as nucleic acid fragments that contain the junction site of the duplicated segment. The detection of additional mutations in the CFTR gene are also provided.
    Type: Grant
    Filed: January 8, 2018
    Date of Patent: May 19, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Feras Hantash
  • Patent number: 10655128
    Abstract: Aspects of the invention provide single stranded oligonucleotides for activating or enhancing expression of MECP2. Further aspects provide compositions and kits comprising single stranded oligonucleotides for activating or enhancing expression of MECP2. Methods for modulating expression of MECP2 using the single stranded oligonucleotides are also provided. Further aspects of the invention provide methods for selecting a candidate oligonucleotide for activating or enhancing expression of MECP2.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: May 19, 2020
    Assignees: Translate Bio MA, Inc., The General Hospital Corporation
    Inventors: Arthur M. Krieg, Romesh Subramanian, James McSwiggen, Jeannie T. Lee
  • Patent number: 10656159
    Abstract: The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: May 19, 2020
    Assignee: HUNAN SKYWORLD BIOTECHNOLOGIES CO
    Inventors: Xiujuan Hao, Isaac A Angres
  • Patent number: 10648035
    Abstract: The present invention relates to the field of gastric cancer. More specifically, the present invention provides methods and compositions for diagnosing and treating gastric cancer. In a specific embodiment, a method for diagnosing gastric cancer or a likelihood thereof in a patient comprises the steps of (a) providing a sample from the patient; (b) measuring the methylation levels of one or more biomarkers in the sample collected from the patient; and (c) predicting gastric cancer in the patient if the biomarkers are hypermethylated. In a more specific embodiment, the one or more biomarkers comprises tight junction protein claudin-1 1 (CLD-N11), the transcription factor BarH-like homeobox (BARX1), basonuclin1 (BNC1), Coagulation factor C homolog (COCH), filamin C gamma (FLNC), cytoglobin B (CYGB), glutamine-fructose-6-phospahe-transaminase-2 (GFPT2), heat-shock-70 kDa protein-6 (HSPA6), snail homolog 1 (SNAL1), skin calmodulin-related factor (SCARF), and tumor protein p53 binding protein 2 (TP53BP2).
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: May 12, 2020
    Assignee: The Johns Hopkins University
    Inventors: Rachana Agarwal, Stephen J. Meltzer
  • Patent number: 10648031
    Abstract: The present invention is directed to novel methods and kits to be employed for preparing adapter-ligated amplicons or a sequencing library of a target DNA, respectively.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: May 12, 2020
    Assignee: QIAGEN GmbH
    Inventors: Nan Fang, Dirk Loeffert
  • Patent number: 10647981
    Abstract: Methods of forming a library of polynucleotide templates for DNA sequencing are disclosed. The methods include distributing a nucleic acid sample into different droplets in a set of emulsion droplets. The droplets into which the polynucleotide species are distributed include primers which binds to the polynucleotide species and are extended by a polymerase contained within the droplet. A set of extended primers are created simultaneously in a large number of droplets. An enriched set of extended primers are distributed into a second set of emulsion droplets containing a second primer which is extended by a polymerase contained within the second set of droplets.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: May 12, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: John A. Luckey
  • Patent number: 10648941
    Abstract: In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and a binding probe molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the binding probe. In various examples, the binding probe is an antibody, the Fab domain of an antibody, a protein, a nucleic acid oligomer hybridization probe, or an aptamer. The circuit may further comprise molecular arms used to wire the binding probe to the electrodes. In various embodiments, the circuit functions as a sensor wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as targets interact with the binding probe. In various embodiments, the circuit provides a means to measure the presence, absence, or concentration of an analyte in a solution.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: May 12, 2020
    Assignee: Roswell Biotechnologies, Inc.
    Inventors: Barry L. Merriman, Venkatesh Alagarswamy Govindaraj, Paul Mola, Tim Geiser, Gina Costa
  • Patent number: 10648011
    Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: May 12, 2020
    Assignees: Ikeda Food Research Co., ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
  • Patent number: 10648037
    Abstract: The present invention discloses a method for non-invasively detecting EGFR gene mutations in subjects, comprising the following steps: designing primers according to EGFR gene exons; extracting plasma DNAs in subjects; connecting the extracted plasma DNAs with tagging linkers; PCR pre-amplifying the tagging linkers connected plasma DNAs; cyclising the pre-amplified DNAs to obtain cyclised DNAs; PCR amplifying the cyclised DNAs using the designed primers; and high throughput sequencing the PCR amplified product and analyzing the EGFR gene mutations. The present invention also discloses a corresponding kit.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: May 12, 2020
    Assignee: Berry Genomics Co., Ltd.
    Inventors: Lina Wang, Lanlan Ru, Tiancheng Li, Yaxi Zhang, Jianguang Zhang
  • Patent number: 10640770
    Abstract: The present invention relates to RNAi agents, e.g., double-stranded RNAi agents, targeting the hepatitis D virus (HDV) genome, and methods of using such RNAi agents to inhibit expression of one or more HBV genes and methods of treating subjects having an HDV infection and/or HDV-associated disorder.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: May 5, 2020
    Assignee: Alnylam Pharmaceuticals, Inc.
    Inventors: Gregory Hinkle, Laura Sepp-Lorenzino, Vasant Jadhav, Martin Maier, Muthiah Manoharan, Stuart Milstein, Svetlana Shulga Morskaya, Kallanthottathil G. Rajeev, Huilei Xu
  • Patent number: 10640811
    Abstract: Reagents and methods for the analysis of nucleic acids (e.g. genomic DNA) of circulating microparticles (i.e. microparticles originating from blood) are provided. The methods comprise linking at least two fragments of a target nucleic acid of a circulating microparticle to produce a set of at least two linked fragments of the target nucleic acid. In the methods, fragments of a target nucleic acid may be linked by techniques such as barcoding, partitioning, ligation and/or separate sequencing. The sequencing of a set of linked fragments provides a set of informatically linked sequence reads corresponding to the sequences of fragments from a single microparticle.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: May 5, 2020
    Assignee: CS Genetics Limited
    Inventor: Lucas Brandon Edelman
  • Patent number: 10638734
    Abstract: The present invention relates generally to mammalian multipotent adult stem cells (MASC), and more specifically to methods for obtaining, maintaining and differentiating MASC to cells of multiple tissue types. Uses of MASC in the therapeutic treatment of disease are also provided.
    Type: Grant
    Filed: October 19, 2010
    Date of Patent: May 5, 2020
    Assignees: ABT Holding Company, Regents of the University of Minnesota
    Inventors: Leo T. Furcht, Catherine M. Verfaillie, Morayma Reyes
  • Patent number: 10643740
    Abstract: Displaying a comparison of genetic data is disclosed, including receiving an indication of a first individual, receiving an indication of a second individual, retrieving the genotypic information for the first individual and the second individual, comparing the genotypic information of the first individual and the second individual, displaying an indication of the comparison of the genotypic information of the first individual and the second individual graphically. A first graphical symbol is used to display an indication of the genome regions for which the first individual and the second individual are identical. A second graphical symbol is used to display an indication of the genome regions for which the first individual and the second individual are half identical.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: May 5, 2020
    Assignee: 23andMe, Inc.
    Inventors: Linda Avey, Oleksiy Khomenko, Brian Thomas Naughton, Serge Saxonov, Anne Wojcicki, Alexander Wong
  • Patent number: 10640809
    Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: May 5, 2020
    Assignee: EPICENTRE TECHNOLOGIES CORPORATION
    Inventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
  • Patent number: 10633680
    Abstract: This disclosure describes recombinant cells and methods for microbial biosynthesis of ent-atiserenoic acid. Thus, in one aspect, this disclosure describes a recombinant cell genetically modified to exhibit increased biosynthesis of ent-atiserenoic acid compared to a comparable control cell. In some cases, the recombinant cell can include a host cell modified to include at least one heterologous polynucleotide encoding at least one enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. In some cases, the recombinant cell can include a host cell and at least one heterologous enzyme in a biosynthetic pathway that produces ent-atiserenoic acid.
    Type: Grant
    Filed: March 22, 2017
    Date of Patent: April 28, 2020
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Michael Joseph Smanski, Szu-Yi Hsu
  • Patent number: 10633442
    Abstract: Methods for identifying compounds that positively regulate connexin 43 hemichannels.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: April 28, 2020
    Assignee: Board of Regents of the University of Texas System
    Inventors: Jean X. Jiang, Manuel A. Riquelme, Sumin Gu
  • Patent number: 10632170
    Abstract: Methods for treating, preventing or delaying onset of tumor formation and other forms of cancer are disclosed. The methods involve administration of a complement inhibitor to inhibit C5a receptor signaling in the tumor microenvironment.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: April 28, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: John D. Lambris, Maciej M. Markiewski
  • Patent number: 10634658
    Abstract: In one aspect, methods are described herein for the selective detection and quantitative analysis of biological molecule compositions. A method described herein comprises providing a mixture comprising biological molecules, such as DNA, RNA or proteins, complexed with a translocating agent, such as another DNA or protein, and non-complexed biological molecules. The mixture is contacted with a membrane comprising at least one nanopore and an electric field is applied across the nanopore to selectively translocate the biological molecules complexed with the translocating agent through the at least one nanopore. Concentration of the complexed biological molecules is determined based on the translocation rate of said molecules.
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: April 28, 2020
    Assignee: WAKE FOREST UNIVERSITY HEALTH SCIENCES
    Inventor: Adam R. Hall
  • Patent number: 10634597
    Abstract: This present application relates generally to a testing device for selecting a surfactant during oilfield subterranean operation technologies. More specifically, the application relates to providing a testing device for selecting a surfactant depending on the interaction of the surfactant with a combination of variables, including the formation hydrocarbon, source water and formation mineralogy.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: April 28, 2020
    Assignee: Halliburton Energy Services, Inc.
    Inventors: Scott Anthony Klasner, Kurt William Hoeman, Denise Nicole Benoit
  • Patent number: 10630678
    Abstract: Techniques for biochemcally-enabled security/authentication mechanisms are described herein. In an example embodiment, a security system receives a biological sample from a key. The biological sample includes a set of deoxyribonucleic acid (DNA) oligos that represent a code assigned to the key. The set of DNA oligos is sequenced to obtain a set of read sequences. The set of read sequences is then filtered to identify a set of filtered sequences. The set of filtered sequences is matched to sets of expected sequences, where the sets of expected sequences are assigned to respective keys issued for the security system. Access to a resource is then granted or denied based on whether the set of filtered sequences matches with any set from the sets of expected sequences.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: April 21, 2020
    Inventor: Bahram Ghaffarzadeh Kermani
  • Patent number: 10626433
    Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: April 21, 2020
    Assignees: Ikeda Food Research Co., Ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
  • Patent number: 10617759
    Abstract: The invention provides oligonucleotide conjugates including a photolabile crosslinker attached to a cargo moiety, e.g., a therapeutic or diagnostic agent. The invention further provides reagents useful in the preparation of such conjugates and methods of their use.
    Type: Grant
    Filed: March 1, 2017
    Date of Patent: April 14, 2020
    Assignee: Trustees of Boston University
    Inventors: Xue Han, Richie E. Kohman, Susie S. Cha
  • Patent number: 10619160
    Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a RRM2 gene. The invention also relates to a pharmaceutical composition comprising the dsRNA or nucleic acid molecules or vectors encoding the same together with a pharmaceutically acceptable carrier; methods for treating diseases caused by the expression of a RRM2 gene using said pharmaceutical composition; and methods for inhibiting the expression of RRM2 in a cell.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: April 14, 2020
    Assignee: Arrowhead Pharmaceuticals, Inc.
    Inventors: John Frederick Boylan, Birgit Bramlage, Markus Hossbach, John Reidhaar-Olson
  • Patent number: 10619197
    Abstract: The invention provides a method for lyophilizing integrated composition of pyrophosphorolysis activated polymerization (PAP) in an aqueous solution. It also provides lyophilized integrated PAP composition. Except for nucleic acid template, the integrated composition contains all components. For manipulation, simply add nucleic acid template in an aqueous solution to start amplification. In addition to the easy manipulation, the lyophilized integrated composition can be stored for prolonged period at ambiguous temperature.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: April 14, 2020
    Inventors: Shaofeng Ding, Qiang Liu
  • Patent number: 10620228
    Abstract: It is intended to provide a kit or a device for the detection of lung cancer and a method for detecting lung cancer. The present invention provides a kit or a device for the detection of lung cancer, comprising a nucleic acid capable of specifically binding to a miRNA in a sample from a subject, and a method for detecting lung cancer, comprising measuring the miRNA in vitro.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: April 14, 2020
    Assignees: TORAY INDUSTRIES, INC., NATIONAL CANCER CENTER
    Inventors: Hiroko Sudo, Hitoshi Nobumasa, Satoko Kozono, Satoshi Kondou, Junpei Kawauchi, Atsushi Ochiai, Motohiro Kojima
  • Patent number: 10612087
    Abstract: Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of monovalent cations are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: April 7, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Andrei Fedorov, John Lyle, Keith Bjornson, Jeremiah Hanes
  • Patent number: 10616219
    Abstract: Disclosed herein are a number of example embodiments for data management and analysis in connection with life science operations such as flow cytometry. For example, disclosed herein are (1) a networked link between an acquisition computer and a computer performing analysis on the acquired data, (2) modular experiment templates that can be divided into individual components for future use in multiple experiments, and (3) an automated pipeline of experiment elements.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: April 7, 2020
    Assignee: FlowJo, LLC
    Inventors: Michael D. Stadnisky, Jay Almarode
  • Patent number: 10612096
    Abstract: The invention provides compositions and methods for determining the fraction of fetal nucleic acids in a maternal sample comprising a mixture of fetal and maternal nucleic acids. The fraction of fetal nucleic acids can be used in determining the presence or absence of fetal aneuploidy.
    Type: Grant
    Filed: October 20, 2016
    Date of Patent: April 7, 2020
    Assignee: VERINATA HEALTH, INC.
    Inventors: Richard P. Rava, Yue-Jen Chuu, Manjula Chinnappa, David A. Comstock, Gabrielle Heilek, Michael Hunkapiller
  • Patent number: 10603662
    Abstract: Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
    Type: Grant
    Filed: September 7, 2018
    Date of Patent: March 31, 2020
    Assignee: Brandeis University
    Inventors: Seth Fraden, Galder Cristobal-Azkarate
  • Patent number: RE47983
    Abstract: Provided herein are compositions and methods for identifying or quantitating one or more analytes in sample. The composition can comprise an affinity molecule reversibly conjugated to a label moiety via a double-stranded nucleic acid linker or via an adaptor molecule. The affinity molecule and the label moiety can be linked to different strands of the double-stranded nucleic acid linker. Compositions can be used in any biological assays for detection, identification and/or quantification of target molecules or analytes, including multiplex staining for molecular profiling of individual cells or cellular populations. For example, the compositions can be adapted for use in immunofluorescence, fluorescence in situ hybridization, immunohistochemistry, western blot, and the like.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: May 12, 2020
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Xiaohu Gao, Pavel Zrazhevskiy