Nucleic Acid Based Assay Involving A Hybridization Step With A Nucleic Acid Probe, Involving A Single Nucleotide Polymorphism (snp), Involving Pharmacogenetics, Involving Genotyping, Involving Haplotyping, Or Involving Detection Of Dna Methylation Gene Expression Patents (Class 435/6.11)
  • Patent number: 12018314
    Abstract: The present disclosure relates to methods to deconvolute a mixture sample of genetic material from different origins or sources. The disclosed methods can be used in various applications, including, the non-invasive determination of a fetal genome, a fetal -ome (e.g. exome). or other targeted fetal locus from cell-free nucleic acids in maternal plasma or other body fluids; the determination of cancer-associated mutations from cell-free nucleic acids in a body fluid sample that contains a mixture of nucleic acids from normal cells and tumor cells; and quantification of donor cell contamination using a body fluid from a transplantation recipient to monitor and/or predict the outcome of a transplantation procedure.
    Type: Grant
    Filed: July 4, 2016
    Date of Patent: June 25, 2024
    Assignee: Arima Genomics, Inc.
    Inventors: Siddarth Selvaraj, Nathaniel Heintzman, Christian Edgar Laing
  • Patent number: 12016682
    Abstract: The present disclosure provides devices, methods and systems for analyte detection and/or monitoring, e.g., the continuous and/or semi-continuous monitoring of in-vivo analyte concentrations in real time. Generally, the devices, methods and systems of the present disclosure make use of signaling probes; methods or devices for reducing interferent-based fouling of the signaling probes, non-specific binding to the signaling probes and/or occlusion of a sensor surface; and methods and/or devices for providing an adjusted signal based on a detected signal from the signaling probes. Compositions including conformation switching signaling probes are also provided.
    Type: Grant
    Filed: January 29, 2014
    Date of Patent: June 25, 2024
    Assignee: The Regents of the University of California
    Inventors: Brian Scott Ferguson, Hyongsok Tom Soh, David A. Hoggarth
  • Patent number: 12006520
    Abstract: Engineered nucleases are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. This disclosure provides in vitro selection methods that interrogate 1011 DNA sequences for their ability to be cleaved by active nucleases, e.g., ZFNs and TALENs. The method revealed hundreds of thousands of DNA sequences that can be cleaved in vitro by two ZFNs, CCR5-224 and VF2468, which target the endogenous human CCR5 and VEGF-A genes, respectively. Analysis of the identified sites in cultured human cells revealed CCR5-224-induced mutagenesis at nine off-target loci. This disclosure provides an energy compensation model of ZFN specificity in which excess binding energy contributes to off-target ZFN cleavage. It was also observed that TALENs can achieve cleavage specificity similar to or higher than that observed in ZFNs.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: June 11, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: David R. Liu, John Paul Guilinger, Vikram Pattanayak
  • Patent number: 12006533
    Abstract: Cross-contamination of a test sample used to determine cancer is identified using gene sequencing data. Each test sample includes a number of test sequences that may include a single nucleotide polymorphism (SNP) that can be indicative of cancer. The test sequences are be filtered to remove or negate at least some of the SNPs from the test sequences. Negating the test sequences allows more test sequences to be simultaneously analyzed to determine cross-contamination. Cross-contamination is determined by modeling the variant allele frequency for the test sequences as a function of minor allele frequency, contamination level, and background noise. In some cases, the variant allele frequency is based on a probability function including the minor allele frequency. Cross-contamination of the test sample is determined if the determined contamination level is above a threshold and statistically significant.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: June 11, 2024
    Assignee: GRAIL, LLC
    Inventors: Onur Sakarya, Catalin Barbacioru
  • Patent number: 11993813
    Abstract: A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.
    Type: Grant
    Filed: July 6, 2018
    Date of Patent: May 28, 2024
    Assignee: MGI TECH CO., LTD.
    Inventors: Erkai Liu, Wenwei Zhang, Ao Chen, Chongjun Xu
  • Patent number: 11993807
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: May 28, 2024
    Assignee: Nautilus Subsidiary, Inc.
    Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
  • Patent number: 11981957
    Abstract: Biomarkers relevant to neurons in the brain, in particular at single cell levels, are identified by using olfactory neurons as the best surrogates from subjects so as to establish diagnosis, prognosis, and treatment of brain conditions.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: May 14, 2024
    Assignee: The Johns Hopkins University
    Inventors: Akira Sawa, Koko Ishizuka, YeeWen Candace Wu, Youjin Chung, Nao J. Gamo
  • Patent number: 11981958
    Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using DNA templated ligation. For example, provided herein are methods for detecting an gDNA analyte in a biological sample where (i) the first RTL probe and second RTL probe hybridize to adjacent sequences on the gDNA analyte, (ii) enzyme-mediate cleavage of a 5? FLAP results in release of the 5? FLAP, and (iii) the sequence of the 5? FLAP is determined and used to detect the gDNA analyte in the biological sample.
    Type: Grant
    Filed: August 19, 2021
    Date of Patent: May 14, 2024
    Assignee: 10x Genomics, Inc.
    Inventor: Christina Galonska
  • Patent number: 11970694
    Abstract: Provided are linkers suitable for preparing a conjugate of a nucleic acid and a peptide as a translation product thereof in a reconstituted cell-free translation system in genotype-phenotype mapping (display methods), said linkers comprising a single-stranded structure region having a side chain base pairing with the base at the 3?-end of an mRNA at one end and a peptidyl acceptor region containing an amino acid attached to an oligo RNA consisting of a nucleotide sequence of ACCA via an ester bond at the other end, characterized in that the ester bond is formed by using an artificial RNA catalyst. Also provided are display methods using [mRNA]-[linker]-[peptide] conjugates assembled via such linkers.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: April 30, 2024
    Assignee: PeptiDream Inc.
    Inventors: Kenji Kashiwagi, Patrick Reid
  • Patent number: 11963974
    Abstract: The present invention provides a novel antisense oligonucleotide and a composition for preventing or treating glycogen storage disease type Ia. The present invention provides an antisense oligonucleotide which hybridizes with a pre-mRNA sequence derived from a region including at least one of a base at position 42911000, a base at position 42911004, and a base at position 42911005 in a base sequence of human chromosome 17 of GRCh38/hg38 and has activity to inhibit aberrant splicing of pre-mRNA of c.648G>T variant G6PC.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: April 23, 2024
    Assignees: NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT, HIROSHIMA UNIVERSITY, DAIICHI SANKYO COMPANY, LIMITED
    Inventors: Go Tajima, Satoshi Okada, Miyuki Tsumura
  • Patent number: 11965853
    Abstract: An electrochemical sensor incorporates a ferrocenophane which is a compound with at least one bridging group covalently attached to and connecting the two cyclopentadiene rings associated with the same iron atom. This bridging group maybe tetramethylene. As compared to an equivalent sensor with ferrocene, the tolerance of elevated temperature is improved and so is the working life at ambient temperature.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: April 23, 2024
    Assignee: Schlumberger Technology Corporation
    Inventors: Lynne Crawford, Andrew Meredith, Nathan Lawrence, Timothy Jones
  • Patent number: 11958878
    Abstract: The present invention establishes a molecular therapy for glycogen storage disease type Ia. The present invention provides an oligonucleotide of 15-30 bases comprising a nucleotide sequence complementary to die cDNA of G6PC gene with c.648G>T mutation, wherein the oligonucleotide comprises a sequence complementary to a region comprising any site between the 82nd to the 92nd nucleotide from the 5? end of exon 5 of the G6PC gene with c.648C>T mutation, a pharmacologically acceptable salt or solvate thereof. Also provided is a pharmaceutical drug comprising the oligonucleotide, a pharmacologically acceptable salt or solvate thereof (e.g., therapeutic drug for glycogen storage disease type Ia).
    Type: Grant
    Filed: March 5, 2019
    Date of Patent: April 16, 2024
    Assignees: Daiichi Sankyo Company, Limited, Kobe Gakuin Educational Foundation
    Inventors: Makoto Koizumi, Yoshiyuki Onishi, Takeshi Masuda, Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kentaro Ito, Shinnosuke Tsuji, Masafumi Matsuo
  • Patent number: 11952622
    Abstract: Methods for analyzing DNA-containing samples are provided. The methods can comprise isolating a single genomic equivalent of DNA from the DNA-containing sample to provide a single isolated DNA molecule. The single isolated DNA molecule can be subjected to amplification conditions in the presence of one or more sets of unique molecularly tagged primers to provide one or more amplicons. Any spurious allelic sequences generated during the amplification process are tagged with an identical molecular tag. The methods can also include a step of determining the sequence of the one or more amplicons, in which the majority sequence for each code is selected as the sequence of the single original encapsulated target. The DNA-containing sample can be a forensic sample (e.g., mixed contributor sample), a fetal genetic screening sample, or a biological cell.
    Type: Grant
    Filed: July 15, 2014
    Date of Patent: April 9, 2024
    Assignee: The Johns Hopkins University
    Inventors: Andrew B. Feldman, Jeffrey S. Lin, David Weitz, Assaf Rotem
  • Patent number: 11951121
    Abstract: The present invention relates to small interfering RNA (siRNA) molecules against the HTT gene, adeno-associated viral (AAV) vectors encoding siRNA molecules and methods for treating Huntington's Disease (HD) using the siRNA molecules and AAV vectors.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: April 9, 2024
    Assignee: VOYAGER THERAPEUTICS, INC.
    Inventors: Dinah Wen-Yee Sah, Jinzhao Hou, Pengcheng Zhou, Xin Wang, Jochen Deckert, Markus Hossbach
  • Patent number: 11952617
    Abstract: Compositions and methods for quantitative detection of target nucleic acids, such as miRNAs are disclosed. The methods are especially advantageous for single-color multiplex detection of two or more targets simultaneously (e.g., in the same reaction). The methods can involve optional reverse transcription followed by amplification performed with universal primers, fluorophore-labeled detection probes, and quencher oligonucleotides for quenching fluorescence of any detection probe not bound to a target molecule. The methods employ differential stability of detection probe-quencher oligonucleotide complexes, and by extension, differential fluorescence at various temperatures to distinguish between different target molecules.
    Type: Grant
    Filed: March 17, 2023
    Date of Patent: April 9, 2024
    Assignee: KASA BIO, L.L.C.
    Inventors: Kiran Madanahally Divakar, Shashi Bala
  • Patent number: 11946925
    Abstract: In a nanopore sensor, a nanopore disposed in a support structure has a nanopore diameter and nanopore resistance, RPore. A fluidic passage, disposed in fluidic connection between a first fluidic reservoir and the nanopore, has a cross-sectional extent, along at least a portion of the fluidic passage length, that is greater than the diameter of the nanopore and that is less than the fluidic passage length. The fluidic passage has a fluidic passage resistance, RFP, of at least about 10% of the nanopore resistance, RPore, and no more than about 10 times the nanopore resistance, RPore. The nanopore is disposed in fluidic connection between the fluidic passage and a second fluidic reservoir. At least one electrical transduction element is disposed at the fluidic passage and electrically connected to produce an indication of electrical potential local to the fluidic passage.
    Type: Grant
    Filed: April 12, 2022
    Date of Patent: April 2, 2024
    Assignee: President and Fellows of Harvard College
    Inventor: Ping Xie
  • Patent number: 11946043
    Abstract: The present disclosure provides methods for selectively tagging 5-methylcytosine in a DNA sample and using this approach for genome-wide profiling of 5-methylcytosine in a low input DNA sample such as circulating cell-free DNA.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: April 2, 2024
    Assignee: Ludwig Institute for Cancer Research Ltd
    Inventors: Chunxiao Song, Paulina Siejka
  • Patent number: 11938197
    Abstract: Polynucleotides and vectors can be used for the expression of a transgene in cells, such as liver cells. The expression of the transgene from the polynucleotides and vectors can be useful in gene therapy. Various methods can be used for expressing the transgene from the polynucleotides and vectors in liver cells.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: March 26, 2024
    Assignees: THE SYDNEY CHILDREN'S HOSPITALS NETWORK (RANDWICK AND WESTMEAD (INCORPORATING THE ROYAL ALEXANDRA HOSPITAL FOR CHILDREN), CHILDREN'S MEDICAL RESEARCH INSTITUTE
    Inventors: Ian Alexander, Sharon Cunningham
  • Patent number: 11939624
    Abstract: A method of barcoding is provided. The method comprises performing a ligation assay on target nucleic acid molecules that are in or on cells or cell organelles to produce ligation products and adding cell-origination barcodes onto the ligation products or complements thereof by a split-pool barcoding process.
    Type: Grant
    Filed: October 12, 2023
    Date of Patent: March 26, 2024
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11932903
    Abstract: A kit for split-pool barcoding is provided. The kit comprises: a binding agent that binds to a target molecule that is in or on cells or cell organelles and at least two sets of assayable polymer subunit (APS) oligonucleotides. In the kit each set comprises at least 10 unique APS oligonucleotides, the APS oligonucleotides in a set each comprise a sequence that distinguishes the APS oligonucleotides from one another, and the APS oligonucleotides from different sets are configured to link together in an ordered fashion to form all or part of a cell or organelle origination barcode.
    Type: Grant
    Filed: August 11, 2023
    Date of Patent: March 19, 2024
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11932902
    Abstract: A method for adding cell origination barcodes onto beads is provided. The method comprises: splitting a pool of beads into a plurality of reaction volumes, appending pre-made oligonucleotides onto the beads in the reaction volumes, wherein at least some of the reaction volumes each receive an oligonucleotide that contains a sequence that is different from the other oligonucleotides added to the reaction volumes, pooling the beads and repeating the splitting, appending and pooling steps one or more times to produce a pool of beads that comprise the cell origination barcodes. In the one or more repeats the oligonucleotides that are appended are added to previously appended oligonucleotides to form the cell origination barcodes.
    Type: Grant
    Filed: August 11, 2023
    Date of Patent: March 19, 2024
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11931713
    Abstract: A data storage medium is disclosed comprising a solid support matrix including an optional stabilising reagent or reagents in a dry form, for use as a support for artificially synthesised oligonucleotide sequences encoded with data. Preferably the matrix is fibrous (for example cellulose, or glass, fibres) formed into a support of sufficient strength to hold the oligonucleotide sequences. The stabilising reagents are preferably a combination of a weak base, and a chelating agent, optionally, uric acid or a urate salt, and optionally an anionic surfactant.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: March 19, 2024
    Assignee: Global Life Sciences Solutions Operations UK Ltd
    Inventors: Jeffrey Kenneth Horton, Peter James Tatnell, Robert Stone
  • Patent number: 11935628
    Abstract: Determining relative relationships of people who share a common ancestor within at least a threshold number of generations includes: receiving recombinable deoxyribonucleic acid (DNA) sequence information of a first user and recombinable DNA sequence information of a plurality of users; processing, using one or more computer processors, the recombinable DNA sequence information of the plurality of users in parallel; determining, based at least in part on a result of processing the recombinable DNA information of the plurality of users in parallel, a predicted degree of relationship between the first user and a user among the plurality of users, the predicted degree of relative relationship corresponding to a number of generations within which the first user and the second user share a common ancestor.
    Type: Grant
    Filed: November 2, 2022
    Date of Patent: March 19, 2024
    Assignee: 23andMe, Inc.
    Inventors: Lawrence Hon, Serge Saxonov, Brian Thomas Naughton, Joanna Louise Mountain, Anne Wojcicki, Linda Avey
  • Patent number: 11926864
    Abstract: A method of barcoding is provided. The method comprises: providing a population of fixed cells or cell organelles in a first reaction volume, hybridizing oligonucleotide probes to target molecules that are in or on the cells or cell organelles in the first reaction volume, splitting the population of cells or cell organelles into a plurality of second reactions volumes, wherein at least some of the second reaction volumes receive a single fixed cell or cell organelle from the population of fixed cells or fixed cell organelles, and adding cell-specific nucleic acid barcodes onto: the oligonucleotide probes, ligation products comprising the oligonucleotide probes, or complements of the oligonucleotide probes or ligation products, in the plurality of second reaction volumes.
    Type: Grant
    Filed: October 12, 2023
    Date of Patent: March 12, 2024
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11920149
    Abstract: The present invention relates to nucleic acid regulatory elements that are able to enhance diaphragm-specific expression of genes, in particular expression in diaphragm as such, or in combination with expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly diaphragm-directed gene therapy, and for vaccination purposes.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: March 5, 2024
    Assignee: VRIJE UNIVERSITEIT BRUSSEL
    Inventors: Lay Khim Chuah, Thierry Vandendriessche, Warut Tulalamba
  • Patent number: 11913076
    Abstract: The present disclosure provides gene expression profiles that are associated with prostate cancer, including certain gene expression profiles that differentiate between subjects of African and Caucasian descent and other gene expression profiles that are common to subjects of both African and Caucasian descent. The gene expression profiles can be measured at the nucleic acid or protein level and used to stratify prostate cancer based on ethnicity. The gene expression profiles can also be used to identify a subject for prostate cancer treatment. Also provided are kits for diagnosing and prognosing prostate cancer and an array comprising probes for detecting the unique gene expression profiles associated with prostate cancer in subjects of African and/or Caucasian descent.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: February 27, 2024
    Assignee: THE HENRY M. JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE, INC.
    Inventors: Shiv K. Srivastava, Gyorgy Petrovics, Indu Kohaar
  • Patent number: 11906523
    Abstract: Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: February 20, 2024
    Assignee: VENTANA MEDICAL SYSTEMS, INC.
    Inventors: Nelson Alexander, William Day, Jerome W. Kosmeder, II, Mark Lefever, Larry Morrison, Anne M. Pedata, Stacey Stanislaw
  • Patent number: 11896945
    Abstract: High surface area coatings are applied to solid substrates to increase the surface area available for solid-phase synthesis of polymers. The high surface area coatings use three-dimensional space to provide more area for functional groups to bind polymers than an untreated solid substrate. The polymers may be oligonucleotides, polypeptides, or another type of polymer. The solid substrate is a rigid supportive layer made from a material such as glass, a silicon material, a metal material, and plastic. The coating may be thin films, hydrogels, microparticles. The coating may be made from a metal oxide, a high-? dielectric, a low-? dielectric, an etched metal, a carbon material, or an organic polymer. The functional groups may be hydroxyl groups, amine groups, thiolate groups, alkenes, n-alkenes, alkalines, N-Hydroxysuccinimide (NHS)-activated esters, polyaniline, aminosilane groups, silanized oxides, oligothiophenes, and diazonium compounds.
    Type: Grant
    Filed: October 9, 2019
    Date of Patent: February 13, 2024
    Assignee: MICROSOFT TECHNOLOGY LICENSING, LLC
    Inventors: Bichlien Hoang Nguyen, Karin Strauss, Hsing-Yeh Parker
  • Patent number: 11892445
    Abstract: Disclosed herein are devices, systems, and methods for controlling a translocation speed of a molecule through a nanopore. In some embodiments, a speed-control device comprises at least one fluid-retaining surface, a fluid region, a field-responsive fluid coupled to the fluid-retaining surface and situated in the fluid region. In some embodiments, a system comprises the nanopore, the speed-control device, and a field generator for generating a magnetic or electric field across the fluid region. The viscosity of the field-responsive fluid is dependent on a magnitude of the magnetic or electric field across the fluid region and can be controlled by changing a magnitude of the magnetic or electric field across the fluid region.
    Type: Grant
    Filed: December 8, 2021
    Date of Patent: February 6, 2024
    Assignee: Western Digital Technologies, Inc.
    Inventors: Daniel Bedau, Justin P. Kinney
  • Patent number: 11885723
    Abstract: A single dissolving compound forms plural azeotropes, which can be azeotropically vaporized off at various stages of the treatment process, thus maintaining predictable concentrations of the chemicals present. The treatment process can be performed in the absence of formalin or related compounds which can interfere with the preservation of genetic material. A process for preserving a specimen includes using a dissolving compound that can form a plural number of azeotropes, at least one azeotrope being formed between one or more components of the dissolving compound and specimen-supplied water, and at least one azeotrope being formed between different components of the dissolving compound; successively and azeotropically vaporizing off formed azeotropes; and impregnating the specimen with a support medium.
    Type: Grant
    Filed: February 24, 2020
    Date of Patent: January 30, 2024
    Inventor: Steven Wheeler
  • Patent number: 11887699
    Abstract: A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.
    Type: Grant
    Filed: September 16, 2022
    Date of Patent: January 30, 2024
    Assignee: Life Technologies Corporation
    Inventor: Cheng-Zong Bai
  • Patent number: 11874278
    Abstract: Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors. The invention further relates to the application of these polymers in assay methods.
    Type: Grant
    Filed: April 21, 2022
    Date of Patent: January 16, 2024
    Assignee: SIRIGEN II LIMITED
    Inventors: Brent S. Gaylord, Glenn P. Bartholomew, Russell A. Baldocchi, Janice W. Hong, William H. Huisman, Yongchao Liang, Trung Nguyen, Lan T. Tran, Jean M. Wheeler, Adrian Charles Vernon Palmer, Frank Peter Uckert
  • Patent number: 11866786
    Abstract: Methods and kits for identification of lung cancer in a subject based on alterations in DNA methylation at selected genomic loci are provided.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: January 9, 2024
    Assignee: NUCLEIX LTD.
    Inventors: Danny Frumkin, Adam Wasserstrom
  • Patent number: 11869024
    Abstract: A disclosed example includes accessing computer-generated impression records, the computer-generated impression records based on network communications received at a server of a database proprietor from client devices, the computer-generated impression records indicative of accesses to media at the client devices; accessing self-reported demographic data and behavioral data from a database of the database proprietor, the self-reported demographic data and the behavioral data corresponding to user accounts registered with the database proprietor and associated with the client devices; comparing the self-reported demographic data with a probability distribution of higher-accuracy demographic data; determining different adjustments for corresponding ones of self-reported demographics of the self-reported demographic data based on the comparison; adjusting the corresponding ones of the self-reported demographics based on corresponding ones of the different adjustments to generate adjusted user demographic data; a
    Type: Grant
    Filed: December 28, 2022
    Date of Patent: January 9, 2024
    Assignee: The Nielsen Company (US), LLC
    Inventors: Albert R. Perez, Josh Gaunt
  • Patent number: 11857968
    Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.
    Type: Grant
    Filed: June 24, 2021
    Date of Patent: January 2, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Lung-Hsin Hung, Robert Meltzer, Lucas Frenz
  • Patent number: 11851717
    Abstract: A method of diagnosing severe sepsis prior to definitive clinical diagnosis. A pattern of gene expression that correlates strongly with a future diagnosis of severe sepsis and organ failure was identified in patients who had their blood drawn at first clinical presentation. The methods comprise identifying a pattern of two or more polynucleotides, whereby the altered expression of these polynucleotides correlates with prospective and actual sepsis. Also methods of identifying agents for treating sepsis based on the characteristics of this gene expression pattern are provided.
    Type: Grant
    Filed: February 19, 2019
    Date of Patent: December 26, 2023
    Inventor: Robert E. W. Hancock
  • Patent number: 11854669
    Abstract: The present invention relates to method for storing and transmitting information by employing a nucleic acid construct. The nucleic acid construct can include a lock region; a translation key region that corresponds to the identity of a key; and a message region including a nucleic sequence that corresponds to an encrypted message.
    Type: Grant
    Filed: July 16, 2019
    Date of Patent: December 26, 2023
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: George Bachand, Marlene Bachand, Andrew Gomez
  • Patent number: 11840725
    Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: December 12, 2023
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, Mitchell Ray O′Connell, Alexandra East-Seletsky, Spencer Charles Knight, James Harrison Doudna Cate
  • Patent number: 11837340
    Abstract: Methods, apparatuses, and systems having an electronic medical records system which intelligently selects medical findings or related information and maps them to associated genes. An electronic medical records system intelligently selects genes and maps them to associated medical findings or other related information.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: December 5, 2023
    Assignee: MEDICOMP SYSTEMS, INC.
    Inventor: Peter S Goltra
  • Patent number: 11835462
    Abstract: This disclosure relates to compositions and methods for analyzing a tissue section from a biological sample.
    Type: Grant
    Filed: February 10, 2021
    Date of Patent: December 5, 2023
    Assignee: 10x Genomics, Inc.
    Inventor: Felice Alessio Bava
  • Patent number: 11834702
    Abstract: A method for determining a mutation in genomic DNA is described. The method is characterized in that the mutation analysis is performed with genomic DNA, in which at least a part of the cytosines contained therein has previously been converted into uracil or another base with a base pairing behavior or molecular weight distinguishable from that of cytosine.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: December 5, 2023
    Inventor: Dimo Dietrich
  • Patent number: 11827919
    Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: November 28, 2023
    Assignee: The Regents of the University of California
    Inventors: Jennifer A Doudna, Mitchell Ray O'Connell, Alexandra East-Seletsky, Spencer Charles Knight, James Harrison Doudna Cate
  • Patent number: 11786914
    Abstract: A magnetic separation filter has an unsupported magnetically soft material layer having a plurality of pores, and, optionally, a passivation layer disposed on the magnetically soft material layer. The magnetic separation filter may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: October 17, 2023
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: David Issadore, Venkata Yelleswarapu, Jina Ko
  • Patent number: 11788135
    Abstract: As described below, disclosed herein are methods of analyzing DNA methylation in cell-free DNA (cfDNA) and genomic DNA (gDNA) from sequencing data.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: October 17, 2023
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Yaping Liu, Manolis Kellis, Viktor Adalsteinsson, Zhizhuo Zhang
  • Patent number: 11792016
    Abstract: Disclosed examples include accessing a search term from a client device; accessing a first identifier, the first identifier corresponding to a first database proprietor, the first identifier to access first user information corresponding to a user of the client device; accessing a second identifier, the second identifier corresponding to a second database proprietor, the second identifier to access second user information corresponding to the user of the client device; providing the search term, the first identifier, and the second identifier in a message; and transmitting the message to a server.
    Type: Grant
    Filed: September 19, 2022
    Date of Patent: October 17, 2023
    Assignee: The Nielsen Company (US), LLC
    Inventors: John R. Burbank, Madhusudhan Reddy Alla
  • Patent number: 11763916
    Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for using machine learning models for plant biotechnology. One of the methods includes obtaining a network input comprising an image depicting a plurality of plant cells or regions of plant tissue; processing the network input using a machine learning model to obtain an identification of one or more particular biotechnologically-modifiable plant cells or one or more particular biotechnologically-modifiable regions of the plant tissue; excising or delineating the one or more identified plant cells or the one or more identified regions of the plant tissue; and delivering exogenous material to the excised or delineated plant cells or regions of plant tissue.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: September 19, 2023
    Assignee: X Development LLC
    Inventor: Bradley Michael Zamft
  • Patent number: 11753681
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the detection of nucleic acids and proteins. Also provided are methods, devices, systems, and compositions for improved detection and quantification of target molecules using encoded probes. In certain aspects, the methods, devices, and systems provided herein are useful in multiplexed digital assays. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of compartmentalized volumes in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: September 12, 2023
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: Daniel T. Chiu, Jiangbo Yu, Jason E. Kreutz, Jiasi Wang
  • Patent number: 11753677
    Abstract: The present disclosure relates to methods and kits for generating single cell barcodes and imparting them to the constituent molecules within a single cell. Additionally, methods to overlay sample barcode and spatial barcode information onto the single cell barcodes are also described. Generation of single cell barcodes is achieved by labeling the genomic DNA of a cell/nucleus with a small handful, preferably just a one or two cellular barcode probes (CBP) that can be amplified and propagated to label the constituent molecules within the cell. The disclosure finds utility in applications such as characterization of cellular heterogeneity, comprehensive profiling of tissue composition, characterization of adherent cells, discovery of new cell subtypes and functions of individual cells in the context of its microenvironment, and others.
    Type: Grant
    Filed: November 9, 2022
    Date of Patent: September 12, 2023
    Assignee: Encodia, Inc.
    Inventors: Mark S. Chee, Haibiao Gong, Kevin L. Gunderson
  • Patent number: 11753674
    Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.
    Type: Grant
    Filed: July 31, 2020
    Date of Patent: September 12, 2023
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Mark S. Chee, David A. Routenberg
  • Patent number: 11753667
    Abstract: The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double stand DNA useful in a variety of applications including protein expression.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: September 12, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Cheng-Hsien Wu, Lloyd Smith