NY-ESO-1-CONTAINING ARTIFICIAL ADJUVANT VECTOR CELL FOR USE IN TREATMENT OF CANCER
An object of the present invention is to provide clinically applicable aAVC-NY-ESO-1 cells stably expressing NY-ESO-1 in order to use aAVC-NY-ESO-1 cells in treating patients having a NY-ESO-1-expressing cancer. The present invention provides, for example, a human-derived cell comprising a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
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This application is a Divisional of U.S. application Ser. No. 17/781,616, which is the U.S. National Stage of PCT/JP2020/044586, filed Dec. 1, 2020, which claims priority to JP 2019-217704, filed Dec. 2, 2019.
SEQUENCE LISTINGThe instant application contains a Sequence Listing which has been submitted in XML format via Patent Center and is hereby incorporated by reference in its entirety. Said XML copy, created on May 9, 2023, is named sequence.XML and is 17,547 bytes.
TECHNICAL FIELDThe present invention relates to a NY-ESO-1-containing artificial adjuvant vector cell (aAVC-NY-ESO-1 cell) for use in treating a cancer.
BACKGROUND ARTNew York esophageal squamous cell carcinoma 1 (NY-ESO-1), one of the cancer/testis antigens, is found to be expressed in a wide range of tumors such as prostate cancer and bladder cancer. On the other hand, its expression is not seen in normal cells of human adults except for the testis. Therefore, this antigen is considered as a suitable target molecule for cancer therapy, and drugs including vaccines are under development (Non Patent Literatures 1 to 3).
A vaccine G305, which is a mixture of the NY-ESO-1 protein and a glucopyranosyl lipid (GLA) adjuvant, is under clinical trial targeting patients having a NY-ESO-1-expressing cancer, and the induction of NY-ESO-1-specific T cells after vaccination has been confirmed (Non Patent Literature 4).
Fujii et al. have prepared an artificial adjuvant vector cell (aAVC) by modifying human-derived cells to exogenously express CD1d and a cancer antigen, and further pulsing the cells with α-galactosylceramide (α-GalCer) (Patent Literatures 1 to 3). This aAVC activates natural killer T (NKT) cells via its CD1d/α-GalCer complex, and the activated NKT cells produce cytokines such as interferon γ (IFN-γ), which in turn activates natural killer (NK) cells or the like. In mouse models, the administration of aAVC has been shown to have a NK cell-dependent antitumor effect (Patent Literatures 1 to 3). aAVC administered to a mouse is immediately killed by activated NKT cells in vivo, and the fragments of the aAVC are taken up into dendritic cells. The dendritic cells taking up the aAVC fragments present a fragment of the cancer antigen with major histocompatibility complex (MHC) on the cell surface, and induce cancer antigen-specific T cells. In mouse models, the administration of aAVC has been shown to have an antitumor effect via the induction of cancer antigen-specific T cells (Patent Literatures 1 to 3 and Non Patent Literatures 5 and 6). Thus, aAVC has been shown to be capable of strongly inducing two immune mechanisms, i.e., innate immunity activation shown by NK cell activation mediated by NKT cell activation and induction of adaptive immunity shown by induction of cancer antigen-specific T cells.
CITATION LIST Patent Literature[Patent Literature 1] International Publication No. WO 2007/097370
[Patent Literature 2] International Publication No. WO 2010/061930
[Patent Literature 3] International Publication No. WO 2013/018778
An object of the present invention is to provide aAVC stably expressing NY-ESO-1 or a fragment thereof.
Solution to ProblemThe present inventors have conducted considerable diligent studies on the preparation of aAVC expressing NY-ESO-1 as a cancer antigen, and as a result, successfully obtained aAVC stably expressing NY-ESO-1. Specifically, the present inventors have found a problem of constitutively expressed NY-ESO-1, which is reduction in expression level and positive rate with increase in the number of culture days, and solved the problem by diligent studies. Specifically, the present inventors have found that cells inducibly expressing NY-ESO-1 with an inducible promoter (particularly, a drug inducible promoter) show a higher expression level of NY-ESO-1 than in the case of constitutively expressing NY-ESO-1, and successfully obtained aAVC with stably and highly expressing NY-ESO-1 under the control of an inducible promoter (Example 2-2). Specifically, the present invention provides aAVC that can be used for treating a cancer, a method for producing the aAVC, a pharmaceutical composition comprising the aAVC, a method for treating a cancer with the aAVC, etc.
Specifically, the present invention may include the following aspects as a medically or industrially useful substance or method.
[1] A human-derived cell, comprising a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
[2] The cell according to [1], wherein the NY-ESO-1 is human NY-ESO-1.
[3] The cell according to [1] or [2], wherein the CD1d is human CD1d.
[4] The cell according to any one of [1] to [3], wherein the inducible promoter is a drug inducible promoter.
[5] The cell according to [4], wherein the drug inducible promoter is a tetracycline inducible promoter.
[6] The cell according to [5], wherein the tetracycline inducible promoter is TRE3G promoter.
[7] The cell according to any one of [1] to [6], wherein the human-derived cell is a normal cell.
[8] The cell according to [7], wherein the human-derived cell is a cell derived from a human embryonic kidney cell 293 (HEK293) cell.
[9] The cell according to any one of [1] to [8], expressing CD1d and NY-ESO-1 or a fragment thereof.
[10] The cell according to [9], wherein an expression level of the NY-ESO-1 or the fragment thereof is 245 ng to 45000 ng per 106 cells.
[11] The cell according to [9] or [10], wherein a CD1d ligand is loaded on the surface of the cell.
[12] The cell according to [11], wherein the CD1d ligand is α-GalCer.
[13] A pharmaceutical composition comprising a cell according to [11] or [12].
[14] The pharmaceutical composition according to [13] for use in treating a cancer.
[15] A method for treating a cancer, comprising the step of administering a cell according to [11] or [12] to a subject.
[16] The cell according to [11] or [12] for treating a cancer.
[17] Use of a cell according to [11] or [12] for producing a pharmaceutical composition for use in treating a cancer.
[18] A method for producing a cell for use in treating a cancer, comprising the step of culturing a cell according to any one of [1] to [8] in the presence of an inducer of the inducible promoter, thereby inducing the expression of NY-ESO-1 or a fragment thereof.
[19] The method according to [18], further comprising the step of loading α-GalCer onto the surface of the cell.
[20] A method for producing a cell for use in treating a cancer, comprising the step of:
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- introducing a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter; or
- introducing a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell endogenously expressing CD1d, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
[21] The method according to [20], further comprising the step of culturing the cell obtained in the step in the presence of an inducer of the inducible promoter, thereby inducing the expression of NY-ESO-1 or a fragment thereof.
[22] The method according to [21], further comprising the step of loading α-GalCer onto the surface of the cell.
[23] A method for treating a cancer, comprising the step of administering a cell according to [11] or [12] and an immune checkpoint inhibitor to a subject.
[24] The method according to [23], wherein the immune checkpoint inhibitor is a PD-1 immune checkpoint inhibitor or a CTLA-4 immune checkpoint inhibitor.
[25] A method for producing a pharmaceutical composition for use in treating a cancer, comprising the steps of: - thawing a frozen product comprising a cloned human-derived cell comprising a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter;
- propagating the human cell contained in the frozen product to induce the expression of the NY-ESO-1 or the fragment thereof; and
- loading α-GalCer on the surface of the cell.
The aAVC-NY-ESO-1 cell of the present invention and the pharmaceutical composition of the present invention can be used in treating a cancer.
Hereinafter, the present invention will be described in detail. However, the present invention is not limited by detailed description below. Scientific terms and technical terms used in relation to the present invention have meanings generally understood by those skilled in the art, unless otherwise specified herein.
Artificial Adjuvant Vector CellIn the present specification, the terms “artificial adjuvant vector cell” and “aAVC” have the same meaning and are used interchangeably. The terms mean a cell comprising a polynucleotide encoding CD1d polypeptide (hereinafter, also referred to as “CD1d”) and polynucleotide(s) encoding one or two or more arbitrary cancer antigens or fragments thereof.
In the present specification, the phrase “comprising a polynucleotide” refers to a state in which a cell endogenously has the polypeptide, or the polynucleotide has been introduced to a cell by an exogenous method. In this context, the term “endogenous” or “endogenously” may be used in a meaning usually used by those skilled in the art in such a way that an isolated cell comprises a particular gene or polynucleotide or expresses a protein. The terms “exogenous” or “exogenously” are used interchangeably so as to refer to introduction of an artificially prepared gene or polynucleotide into a cell of interest by an operation such as gene manipulation or gene introduction, and artificial expression of a protein encoded by the gene or the polynucleotide artificially introduced into the cell of interest.
aAVC-NY-ESO-1 Cell of Present InventionThe present invention provides
-
- a human-derived cell comprising
- a polynucleotide encoding CD1d, and
- a polynucleotide encoding NY-ESO-1 or a fragment thereof, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter
(in the present specification, also referred to as an “aAVC-NY-ESO-1 cell”).
The human-derived cell used in the present invention is a normal cell or a cell derived from a cancer tissue, and may endogenously express CD1d.
A cell derived from a human, such as a cell isolated and/or purified from an arbitrary human tissue, a cell derived from a particular cell species, an established cell line derived from a human tissue, or a cell differentiated from a stem cell can be used as the normal cell. The normal cell may have an ability to proliferate. A cell isolated and/or purified from a cancer tissue of a patient, or an established cell line derived from a human cancer tissue can be used as the cell derived from a cancer tissue. In this context, the term “isolation” means separation from a living tissue. The term “purification” means separation of a cell derived from a human from one or more of other components contained in a tissue from which the cell is derived. The isolation and purification of cells can be performed by methods known in the art. In one embodiment, the human-derived cell used in the present invention is a normal cell.
The cell isolated and/or purified from an arbitrary human tissue can be a cell derived from an arbitrary human tissue of stomach, small intestine, large intestine, lung, pancreas, kidney, liver, thymus gland, spleen, prostate, ovary, uterus, bone marrow, skin, muscle, peripheral blood, or the like. The cell isolated and/or purified from an arbitrary human tissue may have an ability to proliferate. In one embodiment, the human-derived cell used in the present invention is a non-hemocyte cell.
The cell derived from a particular cell species is a cell derived from a particular cell species of a human tissue (e.g., epithelial cells, endothelial cells, epidermal cells, stromal cells, fibroblasts, adipose tissues, mammary gland cells, mesangial cells, pancreatic β cells, nerve cells, glial cells, exocrine epithelial cells, endocrine cells, skeletal muscle cells, smooth muscle cells, myocardial cells, osteoblasts, embryonic cells, or immune cells (e.g., dendritic cells, macrophages, and B cells)).
Such an established cell line derived from a human tissue can be prepared by use of a method known to those skilled in the art, or is commercially available. Examples of cell lines include human embryonic kidney cell 293 (HEK293) cells (J. Gen. Virol.; 1977; 36: 59-74), WI-38 cells, SC-01MFP cells, and MRC-5 cells, and cells derived from these cells. In one embodiment, the human-derived cell used in the present invention is a cell derived from a HEK293 cell. In one embodiment, the cell derived from a HEK293 cell is a FreeStyle™ 293-F cell.
The cell differentiated from a stem cell is a cell differentiated from a human-derived induced pluripotent stem cell (iPS cell) or embryonic stem cell (ES cell). iPS cells or ES cells and cells derived from these cells can be prepared by use of methods known to those skilled in the art.
The cell derived from a cancer tissue can be obtained by isolation and/or purification from a cancer tissue of a patient by a method known to those skilled in the art. Alternatively, a human cancer tissue-derived established cell line generally available from an institute such as American Type Culture Collection (ATCC®) by those skilled in the art may be used.
2. NY-ESO-1The aAVC-NY-ESO-1 cell of the present invention comprises a polynucleotide encoding New York esophageal squamous cell carcinoma 1 (NY-ESO-1) or a fragment thereof as a cancer antigen. NY-ESO-1, a protein also called cancer/testis antigen 1 (CTAG1), is found to be expressed in various cancers, but is known to be expressed only in a testis tissue among adult normal tissues.
The NY-ESO-1 used in the present invention may be naturally occurring NY-ESO-1, or may be a modified form thereof as long as the modified form induces NY-ESO-1-specific T cells when administered as aAVC. In one embodiment, the NY-ESO-1 used in the present invention is NY-ESO-1 derived from a mammal (e.g., humans, monkeys, mice, rats, dogs, and chimpanzees). In one embodiment, the NY-ESO-1 is human NY-ESO-1. In one embodiment, the human NY-ESO-1 is a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 2. In one embodiment, the human NY-ESO-1 is a polypeptide consisting of an amino acid sequence having at least 90% or more, 95% or more, 96% or more, 97% or more, 98% or more, or 99% or more identity to a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 2. In one embodiment, the human NY-ESO-1 is a polypeptide consisting of an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO: 2 by the deletion, substitution, insertion, and/or addition of 1 or several, preferably 1 to 10, more preferably 1 to 7, still more preferably 1 to 5, 1 to 3, or 1 or 2 amino acids. The NY-ESO-1 used in the present invention may be a fragment of NY-ESO-1 as long as the fragment induces NY-ESO-1-specific T cells when administered as aAVC. In one embodiment, the fragment of NY-ESO-1 is a fragment of human NY-ESO-1. In one embodiment, the fragment of human NY-ESO-1 is a polypeptide having 20% or more, 30% or more, 40% or more, 50% or more, 55% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, or 95% or more of the length of the amino acid sequence set forth in SEQ ID NO: 2. The induction of NY-ESO-1-specific T cells by administration as aAVC can be evaluated by a method described in, for example, Example 1-2 mentioned later. The polynucleotide encoding NY-ESO-1 or a fragment thereof, used in the present invention can be prepared by designing a nucleotide sequence from the amino acid sequence of the NY-ESO-1 or the fragment thereof used by use of a method known in the art. In one embodiment, the polynucleotide encoding NY-ESO-1 is a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 1.
In the present specification, the term “identity” means a value of Identity obtained from parameters provided as defaults using EMBOSS Needle (Nucleic Acids Res.; 2015; 43: W580-W584). The parameters described above are as follows:
-
- Gap Open Penalty=10
- Gap Extend Penalty=0.5
- Matrix=EBLOSUM62
- End Gap Penalty=false
The aAVC-NY-ESO-1 cell of the present invention comprises a polynucleotide encoding CD1d. CD1d is an MHC class I-like glycoprotein expressed on cell surface. The CD1d used in the present invention may be naturally occurring CD1d or a modified form thereof as long as the CD1d has a function of CD1d when expressed by the human-derived cell. Examples of the function of CD1d include the ability to bind to a CD1d ligand (e.g., α-GalCer). The ability of CD1d to bind to a CD1d ligand may be easily evaluated by those skilled in the art using a method known in the art. Alternatively, the function of CD1d may be evaluated by using the ability of aAVC to activate human NKT cells as an index. This ability to activate human NKT cells can be evaluated in an in vitro evaluation system described in, for example,
In one embodiment, the CD1d used in the present invention is CD1d derived from a mammal (e.g., humans, monkeys, mice, rats, dogs, and chimpanzees). In one embodiment, the CD1d is human CD1d. In one embodiment, the human CD1d is a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 4. In one embodiment, the human CD1d is a polypeptide that consists of an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO: 4 with the deletion, substitution, insertion, and/or addition of 1 to 10, 1 to 7, 1 to 5, 1 to 3, or 1 or 2 amino acids, and has a function of CD1d. In one embodiment, the human CD1d is a polypeptide that consists of an amino acid sequence having at least 90% or more, 95% or more, 96% or more, 97% or more, 98% or more, or 99% or more identity to a polypeptide shown in SEQ ID NO: 4, and has a function of CD1d.
The polynucleotide encoding CD1d, used in the present invention may be an endogenous polynucleotide of the human-derived cell used, or an exogenously introduced polynucleotide. In the case of exogenously introducing the polynucleotide encoding CD1d to the human-derived cell, the polynucleotide can be prepared by designing a nucleotide sequence from the amino acid sequence of the CD1d used by use of a method known in the art. In one embodiment, the polynucleotide encoding CD1d is a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 3.
4. PromoterIn the aAVC-NY-ESO-1 cell of the present invention, the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In the present specification, the phrase “operably linked” means a state in which a promoter is linked to a polynucleotide in order to control the expression of the polypeptide in host cells by the promoter. In the aAVC-NY-ESO-1 cell of the present invention, in the case of exogenously introducing the polynucleotide encoding CD1d to the human-derived cell, the polynucleotide may be operably linked to a promoter. In this case, any of a promoter constitutively promoting expression and an inducible promoter can be used as the promoter for the expression of CD1d.
The term “inducible promoter” is a promoter inducibly promoting expression, and refers to a promoter that can induce the expression of a polynucleotide operably linked to the promoter in the presence of an inducer that drives the promoter (in the present specification, also referred to as an “inducer of the inducible promoter” or simply an “inducer”). Examples of the inducible promoter include heat inducible promoters (e.g., heat shock promoter) and drug inducible promoters. In one embodiment, the inducible promoter is a drug inducible promoter. In the present specification, the term “drug inducible promoter” means a promoter that regulates the expression of a polynucleotide operably linked to the promoter through an inducer drug. Examples of the drug inducible promoter include cumate operator sequence, λ operator sequence (e.g., 12×λOp), and an inducible promoter of a tetracycline gene expression induction system (hereinafter, referred to as a “tetracycline inducible promoter”). The cumate operator sequence is inactive in the presence of CymR repressor, but is dissociated from the CymR repressor in the presence of an inducer cumate to induce the expression of a polynucleotide operably linked to the promoter. The λ operator sequence induces the expression of a polynucleotide operably linked to the promoter in the presence of an inducer, such as coumermycin, which dimerizes an activator (λRep-GyrB-AD) having the ability to activate transcription through dimerization. The tetracycline inducible promoter induces the expression of a polynucleotide operably linked to the promoter in the presence of an inducer tetracycline or a derivative thereof (e.g., doxycycline) and a reverse tetracycline-controlled transactivator (rtTA) (e.g., Tet-On 3G). Examples of the tetracycline inducible promoter include TRE3G promoter. In one embodiment, the drug inducible promoter is a tetracycline inducible promoter. In one embodiment, the tetracycline inducible promoter is TRE3G promoter.
Examples of the promoter constitutively promoting expression include promoters derived from viruses such as CMV (cytomegalovirus), RSV (respiratory syncytial virus), and SV40 (simian virus 40), actin promoter, and EF (elongation factor) 1α promoter.
In the aAVC-NY-ESO-1 cell of the present invention, the expression of NY-ESO-1 or a fragment thereof can be induced by culturing the cell in the presence of the inducer of the inducible promoter. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention expresses NY-ESO-1 or a fragment thereof. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention expresses CD1d and NY-ESO-1 or a fragment thereof. In one embodiment, the lower limit of the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention may be more than 88 ng, 90 ng or more, 100 ng or more, 150 ng or more, 200 ng or more, 245 ng or more, 250 ng or more, 300 ng or more, 400 ng or more, 500 ng or more, 600 ng or more, 700 ng or more, 800 ng or more, 900 ng or more, 1000 ng or more, 1500 ng or more, 2000 ng or more, 2500 ng or more, 3000 ng or more, 4000 ng or more, 5000 ng or more, 6000 ng or more, 7000 ng or more, 8000 ng or more, 9000 ng or more, or 10000 ng or more, and the upper limit thereof may be 30000 ng or less, 35000 ng or less, 40000 ng or less, 43890 ng or less, 45000 ng or less, 50000 ng or less, 55000 ng or less, or 60000 ng or less, per 106 cells. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 89 ng to 50000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 89 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 89 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 89 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 89 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 106 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 106 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 106 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 106 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 245 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 245 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 245 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 245 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 1000 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 1000 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 1000 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell of the present invention is 1000 ng to 35000 ng.
5. CD1d LigandIn one embodiment, in the aAVC-NY-ESO-1 cell of the present invention, a CD1d ligand may be loaded on the cell surface. In the present specification, the phrase “CD1d ligand has been loaded on the cell surface” refers to a state in which the CD1d ligand is bound to the surface of the aAVC cell. The loading of the CD1d ligand onto cell surface may be performed by pulsing cells expressing CD1d with the CD1d ligand (e.g., α-GalCer), as described in the section <Method for preparing aAVC-NY-ESO-1 cell of present invention>. In the present specification, the phrase “pulsing with the CD1d ligand” refers to contact of cells with the CD1d ligand in a culture medium. In aAVC pulsed with the CD1d ligand, the CD1d ligand is loaded on all or at least a part of the CD1d molecules.
Examples of the CD1d ligand used in the present invention include α-galactosylceramide (α-GalCer), α-C-galactosylceramide (α-C-GalCer), 7DW8-5, and isoglobotrihexosylceramide (iGb3). In one embodiment, the CD1d ligand is α-GalCer. α-GalCer, which has a chemical name of (2S,3S,4R)-1-O-(α-D-galactosyl)-N-hexacosanoyl-2-amino-1,3,4-octadecanetriol, is a substance registered under CAS RN: 158021-47-7 and set forth in a molecular formula: C50H99NO9 (molecular weight: 858.34). The α-GalCer may be synthesized according to a technique known in the art, or a commercially available product (e.g., α-Galactosylceramide (Funakoshi Co., Ltd., Cat. KRN7000)) may be used.
Method for Preparing aAVC-NY-ESO-1 Cell of Present InventionThe aAVC-NY-ESO-1 cell of the present invention can be prepared by introducing a polynucleotide encoding NY-ESO-1 or a fragment thereof, and, if necessary, a polynucleotide encoding CD1d, to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. The method for preparing the aAVC-NY-ESO-1 cell of the present invention may further comprise the steps of: culturing the aAVC-NY-ESO-1 cell; cloning the cell; and loading a CD1d ligand onto the cell surface.
1. Introduction of Polynucleotide to Human-Derived CellThe aAVC-NY-ESO-1 cell of the present invention can be prepared by introducing a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter (hereinafter, the polynucleotides to be introduced are also referred to as “polynucleotides of interest”). Alternatively, in the case of using a cell endogenously expressing CD1d as the human-derived cell, the aAVC-NY-ESO-1 cell of the present invention can be prepared by introducing a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell endogenously expressing CD1d, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
The polynucleotide encoding CD1d and the polynucleotide encoding NY-ESO-1 or a fragment thereof can be designed and prepared by use of a standard molecular biological and/or chemical method, for example, by obtaining nucleotide sequences encoding amino acid sequences from NCBI RefSeq ID or GenBank Accession numbers. For example, these polynucleotides can be synthesized by use of a phosphoramidite method based on their nucleotide sequences or can be prepared by combining DNA fragments obtained through polymerase chain reaction (PCR) from cDNA libraries.
The introduction of the polynucleotides of interest to the human-derived cell can be performed by use of any method of a non-viral vector system or a viral vector system, such as electroporation, lipofection, or microinjection. In the case of using a method of a non-viral vector system, the polynucleotides of interest may be in a form such as a plasmid vector or cDNA or in a mRNA form.
The plasmid vector or the viral vector can be used as an expression vector for the exogenous expression of CD1d or NY-ESO-1 or a fragment thereof in the human-derived cell. The expression vector is not particularly limited as long as the expression vector permits expression of the polypeptide of interest. The expression vector can be prepared by use of a method known to those skilled in the art. For the expression vector, for example, pcDNA series (Thermo Fisher Scientific Inc.), pALTER®-MAX (Promega Corp.), or pHEK293 Ultra Expression Vector (Takara Bio Inc.) can be used as the plasmid vector, and, for example, lentivirus, adenovirus, retrovirus, or adeno-associated virus can be used as the viral vector. For example, in the case of using lentivirus in the introduction of the polynucleotides of interest to the human-derived cell, the lentivirus can be prepared using pLVSIN-CMV/EF1α vector (Takara Bio Inc.) or pLenti vector (Thermo Fisher Scientific Inc.). When the human-derived cell is caused to exogenously express CD1d and NY-ESO-1 or a fragment thereof, the polynucleotide encoding CD1d and the polynucleotide encoding NY-ESO-1 or a fragment thereof may be introduced to the human-derived cell using one expression vector, or may be introduced thereto using separate expression vectors. The expression vector may also contain a gene capable of serving as a marker (e.g., a drug resistance gene, a gene encoding a reporter enzyme, or a gene encoding a fluorescent protein) for confirming the expression of the gene or the polynucleotide of interest. The expression vector may further contain a start codon and a stop codon, an enhancer sequence, an untranslated region, a splice junction, a polyadenylation site, or a replicable unit, etc.
In one embodiment, the method for preparing the aAVC-NY-ESO-1 cell of the present invention comprises the step of introducing an expression vector comprising a polynucleotide encoding CD1d and an expression vector comprising a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment, the method for preparing the aAVC-NY-ESO-1 cell of the present invention comprises the step of introducing an expression vector comprising a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment, the method for preparing the aAVC-NY-ESO-1 cell of the present invention comprises the step of introducing an expression vector comprising a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell endogenously expressing CD1d, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment as to these methods, the expression vector is a plasmid vector or a viral vector. In one embodiment, the expression vector is a viral vector. In one embodiment, the viral vector is a lentivirus vector.
In one embodiment, the aAVC-NY-ESO-1 cell of the present invention is a cell prepared by introducing an expression vector comprising a polynucleotide encoding CD1d and an expression vector comprising a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention is a cell prepared by introducing an expression vector comprising a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention is a cell prepared by introducing an expression vector comprising a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell endogenously expressing CD1d, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment as to these cells, the expression vector is a plasmid vector or a viral vector. In one embodiment, the expression vector is a viral vector. In one embodiment, the viral vector is a lentivirus vector. In one embodiment, the prepared aAVC-NY-ESO-1 cell has the polynucleotide encoding CD1d and the polynucleotide encoding NY-ESO-1 or a fragment thereof extrachromosomally or on genomic DNA of the cell nucleus, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter. In one embodiment, the prepared cell has the polynucleotide encoding CD1d and the polynucleotide encoding NY-ESO-1 or a fragment thereof on genomic DNA of the cell nucleus, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
2. Culture of aAVC-NY-ESO-1 CellThe aAVC-NY-ESO-1 cell prepared by the method described above can be further caused to proliferate by culture. The cell culture for the maintenance or proliferation of the aAVC-NY-ESO-1 cell is performed by a method known in the art. For example, MEM medium (Science; 1952; 122: 501), DMEM medium (Virology; 1959; 8: 396-397), RPMI1640 medium (J. Am. Med. Assoc.; 1967; 199: 519-524), 199 medium (Proc. Soc. Exp. Biol. Med.; 1950; 73: 1-8), FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific Inc., Cat. 12338022), CD 293 Medium (Thermo Fisher Scientific Inc., Cat. 11913019), or Expi293™ Expression Medium (Thermo Fisher Scientific Inc., Cat. A1435101) can be used as a basal medium. The culture medium can contain, for example, serum (e.g., fetal bovine serum), a serum replacement (e.g., KnockOut Serum Replacement: KSR), a fatty acid or a lipid, an amino acid, vitamin, a growth factor, a cytokine, an antioxidant, 2-mercaptoethanol, pyruvic acid, a buffer, an inorganic salt, or an antibiotic. In one embodiment, the medium for use in culture is a serum-free medium or a chemically defined medium.
The culture conditions (e.g., culture conditions such as culture time, temperature, medium pH, and CO2 concentration) can be appropriately selected by those skilled in the art. The medium pH is preferably approximately 6 to 8. The culture temperature is not particularly limited and is, for example, approximately 30 to 40° C., preferably approximately 37° C. The CO2 concentration is approximately 1 to 10%, preferably approximately 5%. The culture time is not particularly limited, and the culture is performed for approximately 15 to 336 hours. If necessary, aeration or stirring may be performed.
At the time of culture of the aAVC-NY-ESO-1 cell, the expression of NY-ESO-1 or a fragment thereof may be induced by contacting the aAVC-NY-ESO-1 cells with an inducer of the inducible promoter. In one embodiment, the method for preparing the aAVC-NY-ESO-1 cell comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of an inducer of the inducible promoter, thereby inducing the expression of NY-ESO-1 or a fragment thereof. In one embodiment, the inducible promoter is a drug inducible promoter, and the preparation method comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of an inducer of the drug inducible promoter. In the case of using a drug inducible promoter, the expression of a gene or a polynucleotide operably linked to the drug inducible promoter may be induced by culturing the cell in a medium supplemented with an inducer drug such as tetracycline, doxycycline, cumate, or coumermycin. This step can be performed in accordance with a gene induction method using a general gene induction system. In one embodiment, the drug inducible promoter is a tetracycline inducible promoter, and the preparation method comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of a tetracycline or a derivative thereof and rtTA. In one embodiment, the tetracycline inducible promoter is TRE3G promoter, and the preparation method comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of tetracycline or a derivative thereof and Tet-On 3G. The expression levels of the CD1d and the NY-ESO-1 or the fragment thereof in the obtained aAVC-NY-ESO-1 cell can be measured by a method known to those skilled in the art, such as Western blot, ELISA, or flow cytometry using an anti-CD1d antibody or an anti-NY-ESO-1 antibody.
3. Cloning of aAVC-NY-ESO-1 CellIn the case of introducing a polynucleotide to cells, there coexist cells to which the polynucleotide of interest has been introduced and cells to which the polynucleotide of interest has not been introduced. Further, in the case of integrating a polynucleotide of interest onto genomic DNA of a cell nucleus, the integration location also differs depending on cells. Thus, those cells may be a heterogeneous population. In the present specification, the heterogeneous population of cells is referred to as a “cell pool”. Single cells to which the polynucleotide encoding CD1d and the polynucleotide encoding NY-ESO-1 or a fragment thereof have been introduced, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter (hereinafter, referred to as “cloned cells”) can be obtained by a method for obtaining cells having a single genotype from the cell pool (in the present specification, referred to as “cloning”) and caused to proliferate, thereby obtaining a cell population having a single genotype (hereinafter, referred to as a “population of cloned cells”). The cloning of the aAVC-NY-ESO-1 cell can be carried out by use of a method well known to those skilled in the art. For example, a limiting dilution method, a single-cell sorting method, or a colony pickup method can be used. In one embodiment, the method for cloning the aAVC-NY-ESO-1 cell may employ one method or a combination of two or more methods selected from a limiting dilution method, a single-cell sorting method, and a colony pickup method. In one embodiment, the method for cloning the aAVC-NY-ESO-1 cell is a limiting dilution method.
For each clone of aAVC-NY-ESO-1 cells obtained by the above method, by contacting a part of the cells of the clone with an inducer of an inducible promoter and measuring the expression level of CD1d and NY-ESO-1 or a fragment thereof in each clone, a population of cloned aAVC-NY-ESO-1 cells that show the desired expression levels for CD1d and NY-ESO-1 or fragments thereof in the presence of the inducer can be selected. The selected population of cloned cells may be preserved in a frozen state (also referred to as “frozen product”) and used as a research cell bank (RCB). The cells of RCB may be cultured for proliferation, then frozen, and used as a master cell bank (MCB). The cells of MCB may be cultured for proliferation, then frozen, and used as a working cell bank (WCB). The cells of WCB may be cultured and used as a pharmaceutical raw material. Each cell bank may contain a cryopreserving agent. Each cell bank may be dispensed to a plurality of containers (e.g., 2 to 10000 containers, for example, 10 to 1000 containers, for example, 100 to 500 containers) and stored in a frozen state. The thawing, culture, and contact with the inducer, of the cloned aAVC-NY-ESO-1 cells, and the measurement of the expression levels of the CD1d and the NY-ESO-1 or the fragment thereof can each be performed by a method known to those skilled in the art. The culture, the contact with the inducer, and the measurement of the expression levels can be performed by use of, for example, a method described in “2. Culture of aAVC-NY-ESO-1 cell”. In one embodiment, the culture and contact with the inducer, of the cloned aAVC-NY-ESO-1 cells, and the measurement of the expression levels of the CD1d and the NY-ESO-1 or the fragment thereof can be performed after thawing of RCB and WCB.
In one embodiment, the lower limit of the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is more than 88 ng, 90 ng or more, 100 ng or more, 150 ng or more, 200 ng or more, 245 ng or more, 250 ng or more, 300 ng or more, 400 ng or more, 500 ng or more, 600 ng or more, 700 ng or more, 800 ng or more, 900 ng or more, 1000 ng or more, 1500 ng or more, 2000 ng or more, 2500 ng or more, 3000 ng or more, 4000 ng or more, 5000 ng or more, 6000 ng or more, 7000 ng or more, 8000 ng or more, 9000 ng or more, or 10000 ng or more, and the upper limit thereof is 30000 ng or less, 35000 ng or less, 40000 ng or less, 43890 ng or less, 45000 ng or less, 50000 ng or less, 55000 ng or less, or 60000 ng or less as the upper limit value, per 106 cells. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 89 ng to 50000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 89 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 89 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 89 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 89 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 106 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 106 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 106 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 106 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 245 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 245 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 245 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 245 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 1000 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 1000 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 1000 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the resulting aAVC-NY-ESO-1 cell is 1000 ng to 35000 ng.
4. Loading of CD1d Ligand onto aAVC-NY-ESO-1 CellAn aAVC-NY-ESO-1 cell with a CD1d ligand loaded thereon can be prepared by pulsing the aAVC-NY-ESO-1 cell with the CD1d ligand. The conditions for pulsing with the CD1d ligand (e.g., the timing of adding the CD1d ligand to a cell culture medium, the concentration of the CD1d ligand in the culture medium and culture time) can be appropriately adjusted by those skilled in the art in consideration of the cell used and culture conditions. The concentration of the CD1d ligand to be added to the culture medium for the aAVC-NY-ESO-1 cell is not particularly limited and can be appropriately selected within the range of, for example, 1 ng/mL to 10000 ng/mL.
In one embodiment, the CD1d ligand is α-GalCer. In one embodiment, the loading of the CD1d ligand onto the aAVC-NY-ESO-1 cell may be performed before contact of the cell with the inducer. In one embodiment, the loading of the CD1d ligand onto the aAVC-NY-ESO-1 cell may be performed after contact of the cell with the inducer. In one embodiment, the loading of the CD1d ligand onto the aAVC-NY-ESO-1 cell may be performed at the same time with contact of the cell with the inducer. In these embodiments, the aAVC-NY-ESO-1 cell is preferably a cloned cell.
In the thus-prepared aAVC-NY-ESO-1 cell expressing CD1d and NY-ESO-1 or a fragment thereof and with CD1d ligand loaded thereon, the cell proliferation may be arrested by an artificial method. The method for arresting the proliferation of the cell is not particularly limited. For example, a method of arresting cell proliferation by exposure to radiation of a radioactive ray (e.g., X-ray), or a method of adding a drug such as mitomycin C may be used. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention may be a cell expressing CD1d and NY-ESO-1 or a fragment thereof with its proliferation arrested. In one embodiment, the aAVC-NY-ESO-1 cell of the present invention may be a cell expressing CD1d and NY-ESO-1 or a fragment thereof with a CD1d ligand loaded thereon and its proliferation arrested.
Pharmaceutical Composition, Etc. of Present InventionThe present invention also provides a pharmaceutical composition comprising the aAVC-NY-ESO-1 cell of the present invention. In the pharmaceutical composition, the aAVC-NY-ESO-1 cell expresses CD1d and NY-ESO-1 or a fragment thereof, and has a CD1d ligand loaded on the surface of the cell. In one embodiment, the pharmaceutical composition is a pharmaceutical composition for use in treating a cancer. In one embodiment, the CD1d ligand is α-GalCer. The pharmaceutical composition can be prepared by a commonly used method using an excipient commonly used in the art, i.e., an excipient for a drug, a carrier for a drug, or the like. For the formulation of the pharmaceutical composition, an excipient, a carrier, an additive, or the like appropriate for its dosage form can be used within a pharmaceutically acceptable range. Examples of the dosage form of the pharmaceutical composition include parenteral agents such as injections and agents for drip infusions. In one embodiment, the pharmaceutical composition of the present invention may comprise a frozen product of the aAVC-NY-ESO-1 cell. In one embodiment, the pharmaceutical composition of the present invention may comprise a frozen product of the aAVC-NY-ESO-1 cell and a cryopreserving agent. In one embodiment, the pharmaceutical composition of the present invention may comprise a suspension of the aAVC-NY-ESO-1 cell. In one embodiment, the pharmaceutical composition of the present invention may comprise a suspension of the aAVC-NY-ESO-1 cell and a cryopreserving agent. The present invention also provides use of the aAVC-NY-ESO-1 cell of the present invention for manufacturing a pharmaceutical composition for use in treating a cancer, wherein the cell expresses CD1d and NY-ESO-1 or a fragment thereof, and has a CD1d ligand loaded on the surface of the cell. In one embodiment, the CD1d ligand is α-GalCer. The present invention also provides the aAVC-NY-ESO-1 cell of the present invention for treating a cancer, wherein the cell expresses CD1d and NY-ESO-1 or a fragment thereof, and has a CD1d ligand loaded on the surface of the cell. In one embodiment, the CD1d ligand is α-GalCer.
The present invention also provides a method for treating a cancer, comprising the step of administering the aAVC-NY-ESO-1 cell of the present invention to a subject. In the method, the aAVC-NY-ESO-1 cell expresses CD1d and NY-ESO-1 or a fragment thereof, and has a CD1d ligand loaded on the surface of the cell. In one embodiment, the CD1d ligand is α-GalCer. In the present specification, the term “subject” is a mammal (e.g., humans, monkeys, mice, rats, dogs, and chimpanzees). In one embodiment, the subject is a human. In the present specification, the term “treatment” is used in a meaning including therapeutic treatment and prophylactic treatment. In the case of administering the aAVC-NY-ESO-1 cell to a subject, the aAVC-NY-ESO-1 cell can be administered in the form of a pharmaceutical composition comprising this cell and a pharmaceutically acceptable excipient to the subject. The dose of the aAVC-NY-ESO-1 cell to the subject and the number of doses thereof can be appropriately adjusted according to the type, position, and severity of the cancer, the age, body weight and condition of the subject to be treated, etc. For example, 1×103 cells/kg to 1×109 cells/kg per dose to the subject can be used as the dose of the aAVC-NY-ESO-1 cell. As for a method for administering the aAVC-NY-ESO-1 cell to the subject, the cell can be administered by, for example, intravenous, intratumoral, intradermal, subcutaneous, intramuscular, intraperitoneal, or intra-arterial injection or drip infusion. The treatment method of the present invention can be used in combination with an additional cancer therapy method. Examples of the additional cancer therapy method include surgery, radiotherapy, hematopoietic stem cell transplantation, and therapy with other anticancer agents.
Examples of the cancer to be targeted by treating the present invention include, but are not particularly limited to: blood cancers such as acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin's lymphoma, non-Hodgkin's lymphoma, B cell lymphoma, multiple myeloma, and T cell lymphoma; solid cancers such as myelodysplastic syndrome, adenocarcinoma, squamous cell carcinoma, adenosquamous carcinoma, anaplastic cancer, large-cell cancer, non-small cell lung cancer, small-cell lung cancer, mesothelioma, skin cancer, breast cancer, prostate cancer, bladder cancer, vaginal cancer, neck cancer, head and neck cancer, uterine cancer, cervical cancer, liver cancer, gallbladder cancer, bile duct cancer, kidney cancer, pancreatic cancer, lung cancer, colon cancer, large intestinal cancer, rectum cancer, small intestinal cancer, stomach cancer, esophageal cancer, testis cancer, ovary cancer, and brain tumor; cancers of bone tissues, cartilage tissues, adipose tissues, muscle tissues, vascular tissues and hematopoietic tissues; sarcomas such as chondrosarcoma, Ewing's sarcoma, malignant hemangioendothelioma, malignant schwannoma, osteosarcoma, and soft tissue sarcoma; and blastomas such as hepatoblastoma, medulloblastoma, nephroblastoma, neuroblastoma, pancreatoblastoma, pleuropulmonary blastoma, and retinoblastoma. In one embodiment, the cancer to be targeted by treating the present invention is a NY-ESO-1-positive cancer.
In the pharmaceutical composition, the use, the cell, and the cancer treatment method of the present invention, the lower limit of the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell may be, for example, more than 88 ng, 90 ng or more, 100 ng or more, 150 ng or more, 200 ng or more, 245 ng or more, 250 ng or more, 300 ng or more, 400 ng or more, 500 ng or more, 600 ng or more, 700 ng or more, 800 ng or more, 900 ng or more, 1000 ng or more, 1500 ng or more, 2000 ng or more, 2500 ng or more, 3000 ng or more, 4000 ng or more, 5000 ng or more, 6000 ng or more, 7000 ng or more, 8000 ng or more, 9000 ng or more, or 10000 ng or more, and the upper limit thereof may be 30000 ng or less, 35000 ng or less, 40000 ng or less, 43890 ng or less, 45000 ng or less, 50000 ng or less, 55000 ng or less, or 60000 ng or less as the upper limit value, per 106 cells. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is more than 88 ng, 90 ng or more, 100 ng or more, 150 ng or more, 200 ng or more, 245 ng or more, 250 ng or more, 300 ng or more, 400 ng or more, 500 ng or more, 600 ng or more, 700 ng or more, 800 ng or more, 900 ng or more, 1000 ng or more, 1500 ng or more, 2000 ng or more, 2500 ng or more, 3000 ng or more, 4000 ng or more, 5000 ng or more, 6000 ng or more, 7000 ng or more, 8000 ng or more, 9000 ng or more, or 10000 ng or more as the lower limit value, and 30000 ng or less, 35000 ng or less, 40000 ng or less, 43890 ng or less, 45000 ng or less, 50000 ng or less, 55000 ng or less, or 60000 ng or less as the upper limit value, per 106 cells. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 89 ng to 50000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 89 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 89 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 89 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 89 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 106 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 106 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 106 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 106 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 245 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 245 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 245 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 245 ng to 35000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 1000 ng to 45000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 1000 ng to 43890 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 1000 ng to 40000 ng. In one embodiment, the expression level of the NY-ESO-1 or the fragment thereof in the aAVC-NY-ESO-1 cell is 1000 ng to 35000 ng.
Method for Producing Cell or Pharmaceutical Composition for Use in Treatment of CancerThe present invention also provides a method for producing a cell or a pharmaceutical composition for use in treating a cancer, comprising the step of contacting the aAVC-NY-ESO-1 cell of the present invention with an inducer of the inducible promoter to induce the expression of NY-ESO-1 or a fragment thereof. In one embodiment, the production method comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of an inducer of the inducible promoter, thereby inducing the expression of NY-ESO-1 or a fragment thereof. In one embodiment, the production method further comprises the step of loading α-GalCer onto the cell surface. The loading of the CD1d ligand onto the cell surface may be performed before, after, or at the same time with contact of the aAVC-NY-ESO-1 cell with the inducer. The present invention also provides use of the aAVC-NY-ESO-1 cell of the present invention in the manufacture of a pharmaceutical composition for use in treating a cancer.
The present invention also provides a method for producing a cell or a pharmaceutical composition for use in treating a cancer, comprising the step of:
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- introducing a polynucleotide encoding CD1d and a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter; or
- introducing a polynucleotide encoding NY-ESO-1 or a fragment thereof to a human-derived cell endogenously expressing CD1d, wherein the polynucleotide encoding NY-ESO-1 or a fragment thereof is operably linked to an inducible promoter.
In one embodiment, the production method further comprises the step of contacting the cell obtained in the step (aAVC-NY-ESO-1 cell) with an inducer of the inducible promoter to induce the expression of NY-ESO-1 or a fragment thereof. In one embodiment, the production method comprises the step of culturing the aAVC-NY-ESO-1 cell in the presence of an inducer of the inducible promoter, thereby inducing the expression of NY-ESO-1 or a fragment thereof. In one embodiment, the production method further comprises the step of loading α-GalCer onto the cell surface. The loading of the CD1d ligand onto the cell surface may be performed before, after, or at the same time with contact of the aAVC-NY-ESO-1 cell with the inducer.
Specific embodiments regarding the cell and the steps, etc. for use in the production method described in this section are as described in the preceding sections <aAVC-NY-ESO-1 cell of present invention> and <Method for preparing aAVC-NY-ESO-1 cell of present invention>.
Combined Use of aAVC-NY-ESO-1 Cell of Present Invention and Immune Checkpoint InhibitorThe present invention also provides a method for treating a cancer, comprising the step of administering the aAVC-NY-ESO-1 cell of the present invention and an immune checkpoint inhibitor to a subject. The present invention also provides a pharmaceutical composition for treating a cancer, comprising the aAVC-NY-ESO-1 cell of the present invention, wherein the pharmaceutical composition is used in combination with an immune checkpoint inhibitor. The present invention also provides the aAVC-NY-ESO-1 cell of the present invention for treating a cancer, wherein the aAVC-NY-ESO-1 cell of the present invention is used in combination with an immune checkpoint inhibitor. The present invention also provides use of the aAVC-NY-ESO-1 cell of the present invention for producing a pharmaceutical composition for use in treating a cancer, wherein the pharmaceutical composition is used in combination with an immune checkpoint inhibitor. In the method, the pharmaceutical composition, the cell or the use, the aAVC-NY-ESO-1 cell of the present invention expresses CD1d and NY-ESO-1 or a fragment thereof, and a CD1d ligand is loaded on the surface of the cell.
In the present specification, the “immune checkpoint inhibitor” means a medicament that stimulates the immunity by canceling the suppression of immune cells caused by an immune checkpoint molecule. Examples of the immune checkpoint molecule include programmed cell death 1 (PD-1), cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), T-cell immunoglobulin domain and mucin domain-3 (TIM-3), lymphocyte activation gene 3 (LAG-3), and V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA). The immune checkpoint to be formed by such a molecule is called PD-1 immune checkpoint, CTLA-4 immune checkpoint, TIM-3 immune checkpoint, LAG-3 immune checkpoint, and VISTA immune checkpoint. The immune checkpoint inhibitor is capable of inhibiting the functions of the immune checkpoint, for example, by binding to an immune checkpoint molecule or a ligand thereof. For example, the PD-1 immune checkpoint inhibitor can inhibit the PD-1 immune checkpoint by inhibiting the binding of PD-1 to PD-L1 or PD-L2. The CTLA-4 immune checkpoint inhibitor can inhibit the CTLA-4 immune checkpoint by inhibiting the binding of CTLA-4 to CD80 or CD86. The TIM-3 immune checkpoint inhibitor can inhibit the TIM-3 immune checkpoint by inhibiting the binding of TIM-3 to galectin-9. The LAG-3 immune checkpoint inhibitor can inhibit the LAG-3 immune checkpoint by inhibiting the binding of LAG-3 to an MHC class II molecule. The VISTA immune checkpoint inhibitor can inhibit the VISTA immune checkpoint by inhibiting the binding of VISTA to VSIG-3/IGSF11. The immune checkpoint inhibitor refers to a substance that can inhibit one or more of immune checkpoints such as PD-1 immune checkpoint, CTLA-4 immune checkpoint, TIM-3 immune checkpoint, LAG3 immune checkpoint, and VISTA immune checkpoint. Examples thereof include antibodies, antigen binding proteins, and low molecular compounds. Some antibodies bind to either a receptor or a ligand. Examples of the antibody inhibiting the PD-1 immune checkpoint include anti-PD-1 antibodies, anti-PD-L1 antibodies, and anti-PD-L2 antibodies. For example, nivolumab, pembrolizumab, MK-3475, AMP-224, pidilizumab, AMP-514, ANB-011, BGB-A317, REGN-2810, HR301210, PF-06801591, JS-001, IBI-308, CBT-501, avelumab, atezolizumab, BMS-936559, LY-3300054, JNJ-61610588, and durvalumab may be used. Examples of the antibody inhibiting the CTLA-4 immune checkpoint include anti-CTLA-4 antibodies, anti-CD80 antibodies, and anti-CD86 antibodies. For example, ipilimumab and tremelimumab may be used. Examples of the antibody inhibiting the TIM-3 immune checkpoint include anti-TIM-3 antibodies and anti-galectin-9 antibodies. For example, MGB453 may be used. Examples of the antibody inhibiting the VISTA immune checkpoint include an antibody selected from the group consisting of anti-VISTA antibodies and anti-VSIG-3/IGSF11 antibodies. For example, JNJ-61610588 may be used. Alternatively, AUPM-170 may be used as the low-molecular compound.
Combined use of the aAVC-NY-ESO-1 cell of the present invention and the immune checkpoint inhibitor may enhance the antitumor effect of the aAVC-NY-ESO-1 cell. In one embodiment, the immune checkpoint inhibitor that may be used in combination with the aAVC-NY-ESO-1 cell of the present invention is a PD-1 immune checkpoint inhibitor. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is an anti-PD-1 antibody, an anti-PD-L1 antibody, or an anti-PD-L2 antibody. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is nivolumab, pembrolizumab, avelumab, atezolizumab, or durvalumab. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is nivolumab or pembrolizumab. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is pembrolizumab. In another embodiment, the immune checkpoint inhibitor that may be used in combination with the aAVC-NY-ESO-1 cell of the present invention is a CTLA-4 immune checkpoint inhibitor. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is an anti-CTLA-4 antibody, an anti-CD80 antibody, or an anti-CD86 antibody. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is ipilimumab or tremelimumab. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is nivolumab or pembrolizumab. In one embodiment, the immune checkpoint inhibitor for combined use with the aAVC-NY-ESO-1 cell of the present invention is ipilimumab.
For example, in the method for treating a cancer, comprising administering the aAVC-NY-ESO-1 cell of the present invention and an immune checkpoint inhibitor to a subject, in one embodiment, the immune checkpoint inhibitor to be administered to a subject is a PD-1 immune checkpoint inhibitor or a CTLA-4 immune checkpoint inhibitor. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is a PD-1 immune checkpoint inhibitor. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is an anti-PD-1 antibody, an anti-PD-L1 antibody, or an anti-PD-L2 antibody. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is nivolumab, pembrolizumab, avelumab, atezolizumab, or durvalumab. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is nivolumab or pembrolizumab. In one embodiment, the immune checkpoint inhibitor that is used in combination with the aAVC-NY-ESO-1 cell of the present invention is pembrolizumab. In another embodiment, the immune checkpoint inhibitor that can be used in combination with the aAVC-NY-ESO-1 cell of the present invention is a CTLA-4 immune checkpoint inhibitor. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is an anti-CTLA-4 antibody, an anti-CD80 antibody, or an anti-CD86 antibody. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is ipilimumab or tremelimumab. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is nivolumab or pembrolizumab. In one embodiment, the immune checkpoint inhibitor to be administered to a subject is ipilimumab.
The aAVC-NY-ESO-1 cell of the present invention and the immune checkpoint inhibitor may be administered concurrently, successively, continuously, or in an overlapped manner. Either of the aAVC-NY-ESO-1 cell of the present invention or the immune checkpoint inhibitor may be administered first. In one embodiment, the administration of the aAVC-NY-ESO-1 cell of the present invention can be started prior to the administration of the immune checkpoint inhibitor. In one embodiment, the administration of the immune checkpoint inhibitor can be started prior to the administration of the aAVC-NY-ESO-1 cell of the present invention. The aAVC-NY-ESO-1 cell of the present invention and the immune checkpoint inhibitor may each independently be administered once or a plurality of times. In one embodiment, an additional anticancer agent such as a chemotherapeutic agent may be further used in combination with monotherapy or concomitant therapy of the aAVC-NY-ESO-1 cell of the present invention and the immune checkpoint inhibitor.
Particular Examples to be referred to will be provided herein for the further understanding of the present invention. However, these examples are intended for mere illustration and do not limit the present invention.
EXAMPLES Example 1: Antitumor Effect of Mouse-Type aAVC(3T3)-NY-ESO-1 Cell Example 1-1: Preparation of Mouse-Type aAVC(3T3)-NY-ESO-1In order to confirm the effect as a cancer vaccine of cancer antigen NY-ESO-1-loaded aAVC in an in vivo evaluation system, mRNAs of the NY-ESO-1 gene and the CD1d gene were introduced to mouse NIH/3T3 cells (ATCC, Cat. CRL-1658) to prepare mouse-type aAVC (also referred to as aAVC(3T3)-NY-ESO-1).
The NY-ESO-1 gene (SEQ ID NO: 1) was prepared by artificial gene synthesis based on the amino acid sequence of human NY-ESO-1 set forth in SEQ ID NO: 2. The NY-ESO-1 gene was amplified by PCR using primers having an added sequence complementary to 15 nucleotides including the HindIII or EcoRI recognition sequence of pGEM®-4Z plasmid (Promega Corp., Cat. P2161). The amplified NY-ESO-1 gene was inserted to the HindIII-EcoRI site of pGEM®-4Z using In-Fusion® HD Cloning Kit (Takara Bio Inc., Cat. 639648). The obtained plasmid was designated as pGEM-4Z-NY-ESO-1 plasmid. The mouse CD1d gene (gene synthesized by artificial gene synthesis based on the amino acid sequence of UniProt: P11609-1 (SEQ ID NO: 6)) consisting of the nucleotide sequence set forth in SEQ ID NO: 5 was inserted to the HindIII-BamHI site of pGEM®-4Z plasmid. The obtained plasmid was designated as pGEM-4Z-mCD1d plasmid. The pGEM-4Z-NY-ESO-1 plasmid and the pGEM-4Z-mCD1d plasmid were cleaved with EcoRI and BamHI, respectively, to linearize these plasmids. NY-ESO-1 mRNA and CD1d mRNA were prepared using the resulting linearized plasmids as templates and using mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (Thermo Fisher Scientific Inc., Cat. AMB13455). NIH/3T3 cells cultured for 2 days in Dulbecco's Modified Eagle medium (Thermo Fisher Scientific Inc., Cat. 10569) containing 10% fetal bovine serum supplemented with 500 ng/mL α-GalCer were harvested. NY-ESO-1 mRNA and CD1d mRNA were added to the cell suspension, followed by electroporation (poring pulse: voltage: 150 V, pulse duration: 8 ms, pulse interval: 50 ms, the number of times: 2, decay rate: 10%, polarity: +, transfer pulse: voltage: 20 V, pulse duration: 50 ms, pulse interval: 50 ms, the number of times: ±5, decay rate: 40%, polarity: +/−) using NEPA21 electroporator (Nepa Gene Co., Ltd.). The cells were harvested and irradiated with 30 Gy of X-ray using an X-ray irradiation apparatus MBR-1520R-3 (Hitachi Power Solutions Co., Ltd.). The cells obtained by the method described above were designated as aAVC(3T3)-NY-ESO-1.
Example 1-2: Ability of aAVC(3T3)-NY-ESO-1 to Induce NY-ESO-1-Specific T Cell in Mouse5×105 cells of aAVC(3T3)-NY-ESO-1 suspended in BICANATE Injection (Otsuka Pharmaceutical Co., Ltd.) were administered to the tail vein of each 6-week-old female C57BL/6J mouse (Charles River Laboratories Japan, Inc.) (n=5). 200 μL of BICANATE Injection was administered to a control group. 7 days after administration, spleen cells were recovered from the mouse, and inoculated at 4×105 cells/well to a plate of mouse IFN-γ single-color enzymatic ELISPOT assay kit (Cellular Technology Limited, Cat. mIFNgp-2M/5). In order to stimulate NY-ESO-1-specific T cells, NY-ESO-1 peptides (PepTivator NY-ESO-1—premium grade, human (Miltenyi Biotec, Cat. 130-095-381)) as overlapping peptides covering the full-length NY-ESO-1 protein sequence or PAP peptide (PepTivator PAP—research grade, human (Miltenyi Biotec, Cat. 130-096-767)) as antigen non-specific peptides were further added thereto, and the cells were cultured at 37° C. for 1 day under 5% CO2 conditions. The number of IFN-γ-producing cells was measured by counting the number of spots on the well bottom. In the mice given aAVC(3T3)-NY-ESO-1, the number of cells producing IFN-γ was increased by NY-ESO-1 peptide stimulation (
5×105 cells of aAVC(3T3)-NY-ESO-1 suspended in BICANATE Injection (Otsuka Pharmaceutical Co., Ltd.) were administered to the tail vein of each 8-week-old female C57BL/6J mouse (Charles River Laboratories Japan, Inc.) (n=10 for each group). 200 μL of BICANATE Injection was administered to a control group. 14 days after administration, 3×105 cells of B16-F10 melanoma cells (ATCC, Cat. CRL-6475) suspended in D-PBS(−) (FUJIFILM Wako Pure Chemical Corp., Cat. 045-29795), or 3×105 cells of B16-F10 melanoma cells expressing the NY-ESO-1 protein (which had been prepared by the introduction of the NY-ESO-1 gene; hereinafter, referred to as B16-NY-ESO-1 or B16-NY-ESO-1 cells) suspended in D-PBS(−) were subcutaneously inoculated to the mouse. The day of inoculation of the cancer cells was set to day 0. Change in tumor volume was measured for 20 days. The administration of aAVC(3T3)-NY-ESO-1 significantly suppressed the enlargement of B16-NY-ESO-1 tumor, whereas this administration did not suppress the enlargement of B16-F10 tumor expressing no NY-ESO-1 (
3×105 cells of B16-NY-ESO-1 cells suspended in D-PBS(−) were subcutaneously inoculated to each 8-week-old C57BL/6J female mouse (Charles River Laboratories Japan, Inc.). 7 days after cell inoculation, the mice were divided into 5 groups each consisting of 10 mice on the basis of their tumor volumes. 5×102 cells/mouse, 5×103 cells/mouse, 5×104 cells/mouse, or 5×105 cells/mouse of aAVC(3T3)-NY-ESO-1 suspended in a vehicle BICANATE Injection were administered to the mice via the tail vein in each group. 200 μL of BICANATE Injection was administered to a control group. The day of administration of the aAVC(3T3)-NY-ESO-1 was set to day 0. Change in tumor volume was measured for 10 days. Since two individuals in the 5×105 cells/mouse administration group died after administration of aAVC(3T3)-NY-ESO-1, the number of the mice was eight for this group on day 0 or later. The progression of B16-NY-ESO-1 tumor was significantly suppressed in the 5×103 cells/mouse or more administration groups (
From the results of Example 1, the mouse-type aAVC(3T3)-NY-ESO-1 cells were confirmed to exhibit a NY-ESO-1-specific antitumor effect by inducing NY-ESO-1-specific T cells in the mouse in vivo evaluation system. In humans as well, human-type aAVC-NY-ESO-1 cells can be expected to show an antitumor effect by inducing natural immunity and also to show an antitumor effect based on acquired immunity by inducing antigen-specific acquired immunity. Therefore, studies on the preparation of human-type aAVC-NY-ESO-1 cells were made, as described below, for the purpose of developing a therapeutic drug showing a NY-ESO-1-specific antitumor effect on human NY-ESO-1-expressing cancer.
Example 2: Preparation of Human-Type aAVC-NY-ESO-1 CellHuman-type aAVC-NY-ESO-1 cells were prepared by the following method: aAVC cells expressing NY-ESO-1, CD1d, and Tet3G were established by preparing lentiviruses using plasmids loading NY-ESO-1, CD1d, and Tet3G genes, respectively, and introducing these genes to FreeStyle 293-F cells (Thermo Fisher Scientific Inc., Cat. R79007) using the lentiviruses.
Example 2-1: Lentivirus Preparation (1) Construction of Plasmid for Lentivirus PreparationA lentivirus plasmid pLVsyn-CMV (SEQ ID NO: 7) was prepared. NY-ESO-1 gene having a 5′-terminally added EcoRI recognition sequence and a 3′-terminally added BamHI recognition sequence in NY-ESO-1 gene (SEQ ID NO: 1) was prepared by artificial gene synthesis. The NY-ESO-1 gene excised with restriction enzymes EcoRI and BamHI was inserted into the EcoRI-BamHI site of pLVsyn-CMV plasmid. The obtained plasmid was designated as pLVsyn-CMV-NY-ESO-1 plasmid. TRE3G promoter excised with restriction enzymes ClaI and EcoRI from TRE3G plasmid prepared by artificial gene synthesis service based on the sequence of pTRE3G (Takara Bio Inc., Cat. 631173) was inserted to pLVsyn-CMV-NY-ESO-1 plasmid from which CMV promoter was removed with restriction enzymes ClaI and EcoRI. The obtained plasmid was designated as pLVsyn-TRE3G-NY-ESO-1 plasmid.
The CD1d gene (SEQ ID NO: 3) was designed on the basis of the amino acid sequence of human CD1d set forth in SEQ ID NO: 4, and prepared by artificial gene synthesis by adding an XhoI recognition sequence 5′-terminally and a NotI recognition sequence 3′-terminally to the gene. The CD1d gene excised with restriction enzymes XhoI and NotI was inserted into the XhoI-NotI site of pLVsyn-CMV plasmid. The obtained plasmid was designated as pLVsyn-CMV-CD1d plasmid.
The Tet-On 3G gene was prepared by artificial gene synthesis by adding an XhoI recognition sequence 5′-terminally and a NotI recognition sequence 3′-terminally to the Tet-On 3G gene sequence of pCMV-Tet3G plasmid (Takara Bio Inc., Cat. 631335). The Tet-On 3G gene excised with restriction enzymes XhoI and NotI was inserted into the XhoI-NotI site of pLVsyn-CMV plasmid. The obtained plasmid was designated as pLVsyn-CMV-Tet3G plasmid.
(2) Lentivirus PreparationLentiviruses with NY-ESO-1, CD1d or Tet3G gene loaded thereon were prepared using the pLVsyn-CMV-NY-ESO-1 plasmid, the pLVsyn-TRE3G-NY-ESO-1 plasmid, the pLVsyn-CMV-CD1d plasmid, and the pLVsyn-CMV-Tet3G prepared in (1). The obtained lentiviruses were designated as CMV-NY-ESO-1-loaded lentivirus, TRE3G-NY-ESO-1-loaded lentivirus, CD1d-loaded lentivirus and Tet3G-loaded lentivirus, respectively.
6 μg of the pLVsyn-CMV-NY-ESO-1 plasmid, the pLVsyn-TRE3G-NY-ESO-1 plasmid, the pLVsyn-CMV-CD1d plasmid, or the pLVsyn-CMV-Tet3G plasmid and 18 μL of ViraPower Lentiviral Packaging Mix (Thermo Fisher Scientific Inc., Cat. K497500) were mixed, and 3 mL of OptiPRO™ SFM (Thermo Fisher Scientific Inc., Cat. 12309019) was added thereto and mixed (A). 288 μL of Lipofectamine 2000 CD Transfection Reagent (Thermo Fischer Scientific Inc., Cat. 12566-014) and 12 mL of OptiPro™ SFM were mixed and left standing for 5 minutes (B). The mixture (A) and 3 mL of the mixture (B) were mixed and left standing at room temperature for 20 minutes. Then, gene introduction was performed by adding 3 mL/plate of the mixed solution of (A) and (B) to Lenti-X™ 293T cells (Takara Bio Inc., Cat. 632180) inoculated at 5×106 cells/plate to Falcon® 100 mm TC-treated Cell Culture Dish (Corning Inc., Cat. 353003) on the previous day. The Lenti-X™ 293T cells were cultured at 37° C. under 5% CO2 conditions in DMEM medium (Thermo Fisher Scientific Inc., Cat. 10569010) containing 10% fetal bovine serum (SAFC Biosciences, Cat. 12007C (γ ray-irradiated product)) and 0.1% gentamycin (Thermo Fisher Scientific Inc., Cat. 15750060). After the gene introduction, the cells were cultured for 2 days under the same conditions as above. A culture supernatant containing each gene-loaded lentivirus was recovered. The culture supernatant was centrifuged at 840×g at 4° C. for 10 minutes. The supernatant was filtered through a 0.45 μm filter (Merck KGaA, Cat. SLHV033RS), mixed with PEG-it™ Virus Precipitation Solution (5×) (System Biosciences, LLC, Cat. LV810A-1) in an amount of ¼ of the amount of the supernatant, and left standing overnight at 4° C. The mixture was centrifuged at 1500×g at 4° C. for 30 minutes for removal of a supernatant, and centrifuged again at 1500×g at 4° C. for 5 minutes to completely remove the supernatant. The pellets were suspended in 500 μL of DPBS (Thermo Fisher Scientific Inc., Cat. 14190144) to obtain CMV-NY-ESO-1-loaded lentivirus, TRE3G-NY-ESO-1-loaded lentivirus, CD1d-loaded lentivirus, and Tet3G-loaded lentivirus.
Example 2-2: Preparation and Cloning of Lentivirus-Infected CellFreeStyle 293-F cells were infected with each lentivirus prepared in Example 2-1. A cell population infected with the CMV-NY-ESO-1-loaded lentivirus and the CD1d-loaded lentivirus was designated as FreeStyle 293F_CMV_NY-ESO-1_CD1d cell pool. A cell population infected with the TRE3G-NY-ESO-1-loaded lentivirus, the CD1d-loaded lentivirus, and the Tet3G-loaded lentivirus was designated as FreeStyle 293F_Tet-on_NY-ESO-1_CD1d cell pool. The cloning of cells from these cell pools was carried out to obtain a plurality of clones derived from each cell pool.
(1) Preparation of FreeStyle 293F_CMV_NY-ESO-1_CD1d Cell PoolFreeStyle 293-F cells were infected with the CD1d-loaded lentivirus and the CMV-NY-ESO-1-loaded lentivirus to obtain FreeStyle 293F_CMV_NY-ESO-1 cells. Two different methods, a method of infecting the cells with the CD1d-loaded lentivirus and the CMV-NY-ESO-1-loaded lentivirus at the same time (cell pool A preparation method) or a method of infecting the cells with the CD1d-loaded lentivirus and the CMV-NY-ESO-1-loaded lentivirus sequentially (cell pool C preparation method), were used as infection methods. The FreeStyle 293-F cells were cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific Inc., Cat. 12338018) containing 0.1% gentamycin.
The cell pool A preparation was performed by the following method: FreeStyle 293-F cells were adjusted to a concentration of 1×106 cells/mL, and inoculated in an amount of 1 mL/well to Falcon® 12-Well Flat Bottom Multiwell Cell Culture Plate with Lid (Corning Inc., Cat. 353043; hereinafter, referred to as a 12-well plate). 50 μL each or 100 μL each of the CD1d-loaded lentivirus and the CMV-NY-ESO-1-loaded lentivirus obtained in Example 2-1 was added to each well. After centrifugation at 540×g at room temperature for 30 minutes, the cells were gently suspended by pipetting, and shake-cultured. After culturing for several days, the cells thus were passaged from the 12-well plate to Corning® Polycarbonate 125 mL Erlenmeyer Flask with Vent Cap (Corning Inc., Cat. 431143; hereinafter, referred to as a 125 mL Erlenmeyer flask), and further passaged at appropriate intervals to obtain cell pool A.
The cell pool C preparation was performed by the following method: FreeStyle 293-F cells were adjusted to a concentration of 1×106 cells/mL, and inoculated in an amount of 1 mL/well to Falcon® 12-Well Flat Bottom Multiwell Cell Culture Plate with Lid. 100 μL of the CD1d-loaded lentivirus obtained in Example 2-1 was added to each well. After centrifugation at 540×g at room temperature for 30 minutes, the cells were gently suspended by pipetting, and shake-cultured. After culturing for several days, the cells thus were passaged from the 12-well plate to a 125 mL flask, and further passaged at appropriate intervals to obtain FreeStyle 293F_CD1d cell pool (referred to as cell pool B). The cell pool B was passaged again to a 12-well plate. 50 μL or 200 μL of the CMV-NY-ESO-1-loaded lentivirus was added to each well. The second lentivirus infection was performed by the same procedures as in the first one. After culturing for several days, the cells thus were passaged from the 12-well plate to a 125 mL Erlenmeyer flask, and further passaged at appropriate intervals to obtain cell pool C. The cell pool A and the cell pool C were used in FreeStyle 293F_CMV_NY-ESO-1_CD1d cell pool.
(2) Preparation of FreeStyle 293F_Tet-on_NY-ESO-1_CD1d Cell PoolThe cell pool B obtained during the course of the cell pool C preparation method of (1) was inoculated again to a 12-well plate. 40 μL each or 200 μL each of the TRE3G-NY-ESO-1-loaded lentivirus and the Tet3G-loaded lentivirus obtained in Example 2-1 was added to each well. Lentivirus infection was performed in accordance with the procedures described in (1). One day later, the cells were introduced from the 12-well plate to a 125 mL Erlenmeyer flask, and further passaged at appropriate intervals to obtain FreeStyle 293F_Tet-on_NY-ESO-1_CD1d cell pool.
(3) NY-ESO-1 Expression Stability and Culture Characteristics in Cell PoolTime courses of NY-ESO-1 expression levels after lentivirus infection (
As shown as to the expression level in
As shown as to the survival rate in
Single-cell cloning was performed by the limiting dilution method from the FreeStyle 293F_CMV_NY-ESO-1_CD1d cell pool and the FreeStyle 293F_Tet-on_NY-ESO-1_CD1d cell pool prepared in (1) and (2). Clones stably expressing all the genes were selected. The cells were inoculated at 1 cell/well to a 96-well plate and passaged according to cell proliferation so that the cells were caused to proliferate. The expression levels of the NY-ESO-1 protein and the CD1d protein in the proliferated cells were measured by ELISA for NY-ESO-1 and flow cytometry for CD1d to select clones stably expressing the NY-ESO-1 and CD1d proteins. The expression of NY-ESO-1 in the clones derived from the FreeStyle 293F_Tet-on_NY-ESO-1_CD1d cell pool was induced via Tet-On System by the addition of doxycycline with a final concentration of 100 ng/mL to the medium, and evaluated. The measurement by ELISA was performed in accordance with a general measurement method of sandwich ELISA using Anti-NY-ESO-1 Antibody as an antibody for immobilization, Anti-CTAG1B antibody as a primary antibody, and Rabbit IgG Horseradish Peroxidase-conjugated Antibody as a secondary antibody. The measurement by flow cytometry was performed in accordance with a general measurement method using APC Mouse Anti-Human CD1d antibody (BD Biosciences, Cat. 563505) and FACSVerse™ (BD Biosciences).
(5) NY-ESO-1 Expression Level of Obtained CloneThe expression level of NY-ESO-1 was measured as to the FreeStyle 293F_CMV_NY-ESO-1_CD1d cell pool-derived or FreeStyle 293F_Tet-on_NY-ESO-1_CD1d cell pool-derived clones obtained in (4). The measurement was carried out by use of the ELISA method described in (4) (
As shown in
3×105 cells of B16-NY-ESO-1 cells suspended in D-PBS(−) were subcutaneously inoculated to each 9-week-old C57BL/6J female mouse (Charles River Laboratories Japan, Inc.). 7 days after cell inoculation, the mice were divided into 4 groups each consisting of 10 mice on the basis of their tumor volumes. 0.1 mg of an anti-PD-1 antibody (InVivoMAb anti-mouse PD-1 (CD279), Bio X Cell, Cat. BE0146) or an isotype antibody (InVivoMAb rat IgG2a isotype control, anti-trinitrophenol, Bio X Cell, Cat. BE0089) was intraperitoneally administered to the mice. The antibody was administered a total of 5 times twice a week at 3- to 4-day intervals. 3 days after the start of antibody administration, 5×104 cells of aAVC(3T3)-NY-ESO-1 suspended in a vehicle BICANATE Injection were administered to the tail vein of the mouse. 200 μL of BICANATE Injection was administered to a control group. The day of administration of the aAVC(3T3)-NY-ESO-1 was set to day 0. Change in tumor volume was measured up to day 11. The administration of aAVC(3T3)-NY-ESO-1+ isotype antibody and the administration of the anti-PD-1 antibody suppressed the progression of B16-NY-ESO-1 tumor. The combined administration of aAVC(3T3)-NY-ESO-1 and the anti-PD-1 antibody more markedly suppressed the progression of B16-NY-ESO-1 tumor than the administration of aAVC(3T3)-NY-ESO-1+isotype antibody and the administration of the anti-PD-1 antibody (
7×105 cells of NY-ESO-1 protein-expressing CT26 colorectal cancer cells (ATCC, Cat. CRL-2638) (hereinafter, referred to as CT26-NY-ESO-1 cells) prepared by the introduction of the NY-ESO-1 gene, suspended in D-PBS(−) were subcutaneously inoculated to each 7-week-old BALB/c female mouse (Charles River Laboratories Japan, Inc.). 14 days after cell inoculation, the mice were divided into 4 groups each consisting of 8 mice on the basis of their tumor volumes. 0.1 mg of an anti-PD-1 antibody was intraperitoneally administered to the mouse. No antibody was administered to an antibody non-administration group. The antibody was administered a total of 4 times twice a week at 3- to 4-day intervals. On the start day of antibody administration, 5×105 cells of aAVC(3T3)-NY-ESO-1 suspended in a vehicle BICANATE Injection were administered to the tail vein of the mouse. 200 μL of BICANATE Injection was administered to a control group. The day of administration of the aAVC(3T3)-NY-ESO-1 was set to day 0. Change in tumor volume was measured up to day 14. The administration of aAVC(3T3)-NY-ESO-1 alone and the administration of the anti-PD-1 antibody alone suppressed the progression of CT26-NY-ESO-1 tumor, whereas the combined use of aAVC(3T3)-NY-ESO-1 administration and the anti-PD-1 antibody more markedly suppressed the progression of CT26-NY-ESO-1 than the administration of each agent alone (
7×105 cells of CT26-NY-ESO-1 cells suspended in D-PBS(−) were subcutaneously inoculated to each 7-week-old BALB/c female mouse (Charles River Laboratories Japan, Inc.). 7 days after cell inoculation, the mice were divided into 4 groups each consisting of 10 mice on the basis of their tumor volumes. 7 days after grouping, 0.15 mg of an anti-CTLA-4 antibody (InVivoMAb anti-mouse CTLA-4 (CD152), Bio X Cell, Cat. BE0164) was intraperitoneally administered to the mouse. No antibody was administered to an antibody non-administration group. The antibody was administered a total of 5 times twice a week at 3- to 4-day intervals. On the start day of antibody administration, 5×105 cells of aAVC(3T3)-NY-ESO-1 suspended in a vehicle BICANATE Injection were administered to the tail vein of the mouse. One individual in the combined use group of AVC(3T3)-NY-ESO-1 cell+anti-CTLA-4 antibody died after administration of aAVC(3T3)-NY-ESO-1. 200 μL of BICANATE Injection was administered to a control group. The day of inoculation of the cancer cells was set to day 0. Change in tumor volume was measured for 32 days. The administration of aAVC(3T3)-NY-ESO-1 alone and the administration of the anti-CTLA-4 antibody alone suppressed the progression of CT26-NY-ESO-1 tumor, whereas the combined use of aAVC(3T3)-NY-ESO-1 and the anti-CTLA-4 antibody more markedly suppressed the progression of CT26-NY-ESO-1 than the administration of each agent alone (
The aAVC-NY-ESO-1 cell of the present invention can be expected to be useful in treating a cancer.
FREE TEXT OF SEQUENCE LISTINGThe nucleotide sequence set forth in SEQ ID NO: 1 of the sequence listing is a nucleotide sequence encoding human NY-ESO-1 protein, and the amino acid sequence set forth in SEQ ID NO: 2 of the sequence listing is an amino acid sequence encoded by the sequence of SEQ ID NO: 1. The nucleotide sequence set forth in SEQ ID NO: 3 of the sequence listing is a nucleotide sequence encoding human CD1d protein, and the amino acid sequence set forth in SEQ ID NO: 4 of the sequence listing is an amino acid sequence encoded by the sequence of SEQ ID NO: 3. The nucleotide sequence set forth in SEQ ID NO: 5 of the sequence listing is a nucleotide sequence encoding mouse CD1d protein, and the amino acid sequence set forth in SEQ ID NO: 6 of the sequence listing is an amino acid sequence encoded by the sequence of SEQ ID NO: 5. The nucleotide sequence set forth in SEQ ID NO: 7 of the sequence listing is the nucleotide sequence of pLVsyn-CMV. These sequences are as described in the description of “Artificial Sequence” in the numeric caption <223> of the sequence listing given below.
Claims
1. A cell, comprising:
- a polynucleotide encoding human CD1d and a polynucleotide encoding human NY-ESO-1 or a fragment thereof,
- wherein the polynucleotide encoding human NY-ESO-1 or a fragment thereof is operably linked to a drug inducible promoter,
- wherein the cell is cultured in vitro in the presence of a drug to express human CD1d and human NY-ESO-1 or a fragment thereof
- wherein the cell expresses human NY-ESO-1 or a fragment thereof at an expression level of 245 ng to 45000 ng per 106 cells,
- wherein a CD1d ligand is loaded on the surface of the cell,
- wherein the CD1d ligand is α-GalCer, and
- wherein the cell is derived from a human embryonic kidney cell.
2. The cell according to claim 1, wherein the drug inducible promoter is a tetracycline inducible promoter.
3. The cell according to claim 2, wherein the tetracycline inducible promoter is a TRE3G promoter.
4. The cell according to claim 1, wherein the cell is cultured in the presence of a tetracycline.
5. The cell according to claim 2, wherein the cell is cultured in the presence of a tetracycline.
6. The cell according to claim 1, wherein the human embryonic kidney cell is a human embryonic kidney cell 293 (HEK293) cell.
7. A pharmaceutical composition comprising a cell according to claim 1.
8. A pharmaceutical composition comprising a cell according to claim 2.
9. A method for producing a cell according to claim 1, comprising culturing the cell in the presence of a drug, thereby inducing the expression of human NY-ESO-1 or a fragment thereof.
10. The method according to claim 9, further comprising loading α-GalCer onto the surface of the cell.
11. The method according to claim 9, wherein the drug inducible promoter is a tetracycline inducible promoter.
12. The method according to claim 11, wherein the cell is cultured in the presence of a tetracycline.
13. The method according to claim 9, wherein the human embryonic kidney cell is a human embryonic kidney cell 293 (HEK293) cell.
14. A method for producing a cell, comprising:
- introducing a polynucleotide encoding human CD1d and a polynucleotide encoding human NY-ESO-1 or a fragment thereof to a cell derived from a human embryonic kidney cell, wherein the polynucleotide encoding human NY-ESO-1 or a fragment thereof is operably linked to drug inducible promoter; or
- introducing a polynucleotide encoding human NY-ESO-1 or a fragment thereof to a cell derived from a human embryonic kidney cell endogenously expressing human CD1d, wherein the polynucleotide encoding human NY-ESO-1 or a fragment thereof is operably linked to a drug inducible promoter.
15. The method according to claim 14, further comprising culturing the cell obtained in the presence of drug, thereby inducing the expression of human NY-ESO-1 or a fragment thereof.
16. The method according to claim 15, further comprising loading α-GalCer onto the surface of the cell.
17. The method according to claim 14, wherein the drug inducible promoter is a tetracycline inducible promoter.
18. The method according to claim 17, wherein the cell is cultured in the presence of a tetracycline.
19. The method according to claim 14, wherein the human embryonic kidney cell is a human embryonic kidney cell 293 (HEK293) cell.
Type: Application
Filed: May 15, 2023
Publication Date: Oct 19, 2023
Applicants: ASTELLAS PHARMA INC. (Chuo-ku, Tokyo), RIKEN (Saitama,)
Inventors: Ayaka AKIBA (Tokyo), Tatsuya OKUDAIRA (Tokyo), Yasuhide MASUHARA (Tokyo), Keisuke OHSUMI (Tokyo), Shinichiro FUJII (Saitama)
Application Number: 18/317,358
