New human translocation associated protein

The present invention provides a human translocation associated protein (Gp25L-H) and polynucleotides which identify and encode Gp25L-H. The invention also provides expression vectors, host cells, antibodies and antagonists. The invention also provides methods for the prevention and treatment of diseases associated with expression of Gp25L-H, as well as diagnostic assays.

Skip to:  ·  Claims  ·  References Cited  · Patent History  ·  Patent History

Claims

1. An is isolated and purified polynucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:1.

2. An isolated and purified polynucleotide sequence consisting of SEQ ID NO:2.

3. An isolated and purified polynucleotide sequence which is complementary to the polynucleotide sequence of claim 1.

4. An expression vector containing the polynucleotide sequence of claim 1.

5. A host cell containing the expression vector of claim 4.

6. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:1, the method comprising the steps of:

a) culturing the host cell of claim 5 under conditions suitable for the expression of the polypeptide; and
b) recovering the polypeptide from the host cell culture.

7. A method for detection of a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:1 in a biological sample containing nucleic acid material, the method comprising the steps of:

a) hybridizing the polynucleotide of claim 3 to the nucleic acid material of the biological sample, thereby forming a hybridization complex;
b) washing the hybridization complex under conditions of at least 0.1.times. saline sodium citrate and 0.5% sodium dodecyl sulfate; and
c) detecting the hybridization complex, wherein the presence of the hybridization complex correlates with the presence of the polynucleotide in the biological sample.
Referenced Cited
Other references
  • Palade, G. "Intracellular aspects of the process of protein synthesis." Science (1975) 189(4200):347-358. Parlati, F. et al., "Saccharomyces cerevisiae CNE1 encodes an endoplasmic reticulum (ER) membrane protein with sequence similarity to calnexin and calreticulin and functions as a constituent of the ER quality control apparatus." J. Biol. Chem. (1995) 270(1): 244-253. Wada, I. et al., "SSR.alpha. and Associated Calnexin Are Major Calcium Binding Proteins of the Endoplasmic Reticulum Membrane." J. Biol. Chem. (1991) 266(29) 19599-19610 (GI 848). Holthuis J. C. M. et al., "Translocon-associated protein TRAP.delta. and a novel TRAP-like protein are coordinately expressed with pro-opiomelanocortin in Xenopus intermediate pituitary." Biochem. J. (1995) 312: 205-213 (GI 1063411). Dominguez, M. (Direct Submission), GenBank Sequence Database (Accession X90872), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894. (GI 996057) (1995). Domingues, M. (Direct Submission), GenBank Sequence Database (Accession X90872), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894. (GI 996056) (1995). Walter, P. et al., "Preparation of microsomal membranes for cotranslational protein translocation." Methods Enzymol. (1983) 96:84-93. Bergeron, J. J. et al., "Calnexin: a membrane-bound chaperone of the endoplasmic reticulum." Trends Biochem. Sci. (1994) 19(3) :124-128.
Patent History
Patent number: 5831052
Type: Grant
Filed: May 7, 1997
Date of Patent: Nov 3, 1998
Assignee: Incyte Pharmaceuticals, Inc. (Palo Alto, CA)
Inventors: Jennifer L. Hillman (San Jose, CA), Neil C. Corley (Mountain View, CA), Surya K. Goli (Sunnyvale, CA)
Primary Examiner: Sheela Huff
Attorney: Lucy J. Sheela Mohan-Peterson Incyte Pharmaceuticals, Inc. Billings
Application Number: 8/852,809
Classifications
Current U.S. Class: 536/231; 435/6; 435/691; 435/693; 435/711; 435/3201; Collagen Or Gelatin (435/273); 435/2523; 536/235; Proteins, I.e., More Than 100 Amino Acid Residues (530/350)
International Classification: C07H 2102; C07H 2104;