DNA encoding a protease-activated receptor 3

Abstract

Disclosed are cDNAs and genomic DNAs encoding protease-activated receptor 3 (PAR3) from mouse and human, and the recombinant polypeptides expressed from such cDNAs. The recombinant receptor polypeptides, receptor fragments and analogs expressed on the surface of cells are used in methods of screening candidate compounds for their ability to act as agonists or antagonists to the effects of interaction between thrombin and PAR3. Agonists are used as therapeutics to treat wounds, thrombosis, atherosclerosis, restenosis, inflammation, and other thrombin-activated disorders. Antagonists are used as therapeutics to control blood coagulation and thereby treating heart attack and stroke. Antagonists mediate inflammatory and proliferative responses to injury as occur in normal wound healing and variety of diseases including atherosclerosis, restenosis, pulmonary inflammation (ARDS) and glomerulosclerosis. Antibodies specific for a protease-activated receptor 3 (or receptor fragment or analog) and their use as a therapeutic are also disclosed.

Claims

1. Substantially pure DNA having the nucleotide sequence selected from the group consisting of SEQ ID NO:1, or degenerate variants thereof, and encoding the amino acid sequence of SEQ ID NO:3; SEQ ID NO:2, or degenerate variants thereof encoding an amino acid sequence comprising the amino acid sequence of SEQ ID NO:3; SEQ ID NO:4, or degenerate variants thereof encoding the amino acid sequence of SEQ ID NO:6; and SEQ ID NO:5, or degenerate variants thereof encoding an amino acid sequence comprising the amino acid sequence of SEQ ID NO:6.

2. A vector comprising the DNA of claim 1.

3. A cell comprising the vector of claim 2.

4. A substantially pure DNA sequence complementary to a DNA sequence selected from the group consisting of SEQ ID NO:1, or degenerate variants thereof, and encoding the amino acid sequence of SEQ ID NO:3; SEQ ID NO:2, or degenerate variants thereof encoding an amino acid sequence comprising the amino acid sequence of SEQ ID NO:3; SEQ ID NO:4, or degenerate variants thereof encoding the amino acid sequence of SEQ ID NO:6; and SEQ ID NO:5, or degenerate variants thereof encoding an amino acid sequence comprising the amino acid sequence of SEQ ID NO:6.