Glucosltransferase gene GTFE from amycolatopsis orientalis

- Eli Lilly and Company

The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode the enzyme, transformed heterologous host cells for expressing the enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode the enzyme.

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Claims

1. A substantially pure glycosyltransferase protein from Amycolatopsis orientalis having the amino acid sequence:

2. A substantially pure protein, as in claim 1 wherein at amino acid position 335 of SEQ ID NO.2 Ser is substituted for Pro.

Referenced Cited
Other references
  • S. K. Chung, et al. "Biosynthetic Studies f Aridicin Antibiotics: Microbial Transformations and Glycosylations by Protoplasts." Journal of Antibiotics 39(5):652-659 (May 1986). M. J. Zmijewski, Jr., and B. Briggs. "Biosynthesis of vancomycin: identification of TDP-glucose: aglycosyl-vancomycin glucosyltransferase from Amycolatopsis orientalis." FEMS Microbiology Letters 5: 129-134 (1989). M. J. Zmijewski, Jr., and J. T. Fayerman. Genetic and Biochemistry of Antibiotic Production Ed. L.C. Vining and C. Stuttard. Butterworth Heinemann, Boston. Chapter 18: "Glycopeptide Antibiotics." pp. 71-83 (1995).
Patent History
Patent number: 5932464
Type: Grant
Filed: Jul 21, 1998
Date of Patent: Aug 3, 1999
Assignee: Eli Lilly and Company (Indianapolis, IN)
Inventors: Richard H. Baltz (Indianapolis, IN), Patricia J. Solenberg (Indianapolis, IN), Patti J. Treadway (Greenwood, IN)
Primary Examiner: Robert A. Wax
Assistant Examiner: Einar Stole
Attorney: Thomas D. Webster
Application Number: 9/120,053
Classifications
Current U.S. Class: Transferase Other Than Ribonuclease (2.) (435/193)
International Classification: C12N 910;